FvH4_3g45790

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001707
Physical Location & Seq
Reverse (-)
37989038 .. 37994037
5000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45790.t4

Sequence Viewer

Length: 2259 bp
ATGGAAGGAGGAAGAACTGAACTGGAGTTTGATGAATTTGAGAAGCTCCTTGGTGAGATACCGAATGCTACTTCACATTCTGAGGAATCCGCCACCAAGAGTGTGCCCTTGAATGGAACCATGTCTCCCATTTGTGTCAACTCCTCTTACAAAGGAGGGCCTCTGAGTAAAAAACTCCATCACAATAATGGGAGGCTTGATGAGGGAAAGAACTCGGTGAAGAAAATTCAAAAATCGCCTGCAAAACGGGTTCAACCAGCAGAGGTATCAAATCTGCCTGATGACCATTCCTTAACATCTGCATTTGCAGGACTGAGCTTTGATGATGGCCAAATCCCAAACTCCTTCAAGCCACAGCTTCCGAATATGGATACTCTTATGGTTGTTCCACCTTTCCAAGCAGCAACCGGCATACCTTGTGGTTTTTATGAGTTTGATGTGACAAACATTGGCCAAGAAAGTTCAAACATGTCAAAATATTCAGAAGAGCTTAAAAAACCTCAAGTTCCTATGGAAAAATTGTCAGCAGCTGTTCCTCTAGCTCATGCTGTACAAGGTTTTCAGTTCCTCTCCAATGTGCATGCTCCTGGTTTGCAGATTCCTCTAACATCTGAACAACAGCAATTCTTTTTGGATGCACAAACTCGTTTTCCATACCTACATTCACAACAACATCAACTTAGTTGGAGGAATATCCAAGAGGAACAATATTTTAGGATGCAGCAGCGGTATGTGTACTTGCAGCAGCTTCATGATCAGCGGTTGGAAGCTCCAAACATGCTTCAAGACAGTGGCAACATTGGCAGTAGACTAACTTGTCCGAACCCTAGGCACCCCAATTTTGAGGTGCCAATTTCTCACCAACTGGAGCAATCTAATCAAGAAAGAGTTTGGAACAATTATGTGATTCCCAGGGGCCCAAACCAATCAAGTCCTGCACTGTCCTACACAGATTTTAACAGGATTCATGCTCTTGACAAAGTGGGAAAACAGAATGTTCCTGAAAAGATTCTAACAAGATCACATGGAATGAACACAATCAAAGCTGTGAAATTTGGTGCTGTTGGAGCAGCGGATGAATCACTTGGTCATGTTAGTCCTAATGGAAAAGTTGTCTCAAATGGTCATCTCCGGCATAACTTATCTACCCCAAATGCTGGAAACTTTCCATTGGATAATGTGAGCACATGGGGTATGTTCCCTGGTATAATGCATCTTAAGAGTACAGATTTGAAGGCATTGCCTCAGAAGTATAATGATACAAAGGCATTGCCTCAGAAATATAGCTCAATGGATGAAATAAGTGGCAGAGTTTATCTCATGGCTAAGGATCAACATGGCTGCCGGTTCTTACAAAGAAAATTTTCTGAAGGAGCACGAAAAGATGTTGAGAATATATTTGTCGAAATCATAGATCACATTGTTGAGCTGATGACAGATCCTTTTGGGAATTACCTCATCCAGAAGCTTCTTGAAGTGTGTGATGATGAGCAGCAAATGCAAATTCTTCATTCAATCACAAAAAAGTCTGGAGAACTTGTTAATATTTCCTGTGATATGCATGGGACAAGGGCAGTTCAAAAGGTTATTGAAACCCTCAAAACTCCAGAGCAGTTTTCCATGATTGTTTCCTCACTGAAGCCTGGGATTGTGAATTTGATCAAGAACACAAATGGCAACCACGTTGCGCAACGCTGCTTGCAGTATTTAACACCTGAATATCGTGAGTTTCTTTTCGAAGCTGCAACTAGTAATTGTATTGAGCTTGCAACCGACCGTCATGGCTGTTGTGTGCTTCAGAAATGTCTTAGTCATTCTGATGGTGAGCAAAGAGACCGGTTGATCTGCAAGATCACTTCCAATGCACTAATCCTTTCCCAAGATCCATTTGGGAACTATGTTGTGCAATTCGTCTTTGAGCTTCAGCTTCCATGGGCGACAGTAGACATTCTTGAGCAGTTGGAGGGTAATTATAGGGATTTGTCTGTGCAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGTCGCACTCGCATCATTCAGGAACTGATAGAGAACATGCGCTTGGATCAAATCATGCAAGACCCTTATGGCAATTATGTTATCCAAGCAGCACTCAGTCAATCAAAGGGAAGCCTTCATTCTAAACTGGTAGAAGCAATAAAACCTCATGTGCCTGTTCTCCGGAGCAGTCCTTATGGGAAGAAAATCCTCTCTACCAATGTTTTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

753

Amino Acids

84.63

Weight (kDa)

6.96

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 437 - 471 1e-08 Pumilio-family RNA binding repeat
PUF PF00806 473 - 506 2.8e-12 Pumilio-family RNA binding repeat
PUF PF00806 585 - 619 6.9e-15 Pumilio-family RNA binding repeat
PUF PF00806 659 - 691 2.2e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2024
AasI GACNNNNNNGTC 1 cut(s) 816
Acc16I TGCGCA 1 cut(s) 1689
Acc36I ACCTGC 1 cut(s) 2024
AccB1I GGYRCC 2 cut(s) 831, 847
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccI GTMKAC 2 cut(s) 808, 1944
AccIII TCCGGA 1 cut(s) 2205
AciI CCGC 4 cut(s) 90, 727, 760, 1073
AclWI GGATC 4 cut(s) 1338, 1433, 1877, 2097
AcoI YGGCCR 2 cut(s) 328, 451
AcsI RAATTY 6 cut(s) 35, 225, 1052, 1361, 1503, 1654
AcuI CTGAAG 4 cut(s) 1389, 1658, 1781, 1907
AfaI GTAC 3 cut(s) 552, 737, 1225
AfiI CCNNNNNNNGG 2 cut(s) 113, 1157
AflII CTTAAG 1 cut(s) 1217
AflIII ACRYGT 1 cut(s) 468
AgeI ACCGGT 1 cut(s) 1836
AhlI ACTAGT 1 cut(s) 1748
AjnI CCWGG 4 cut(s) 586, 911, 1201, 1642
AjuI GAANNNNNNNTTGG 2 cut(s) 2069, 2101
AloI GAACNNNNNNTCC 2 cut(s) 109, 141
Alw21I GWGCWC 2 cut(s) 1187, 1378
Alw26I GTCTC 3 cut(s) 129, 1120, 1827
AlwI GGATC 4 cut(s) 1338, 1433, 1877, 2097
AlwNI CAGNNNCTG 2 cut(s) 530, 2068
Aor13HI TCCGGA 1 cut(s) 2205
AoxI GGCC 4 cut(s) 158, 328, 451, 916
ApaI GGGCCC 1 cut(s) 920
ApoI RAATTY 6 cut(s) 35, 225, 1052, 1361, 1503, 1654
AsiGI ACCGGT 1 cut(s) 1836
Asp700I GAANNNNTTC 2 cut(s) 1008, 1363
AspA2I CCTAGG 1 cut(s) 827
AspLEI GCGC 2 cut(s) 1690, 2085
AspS9I GGNCC 3 cut(s) 158, 916, 917
AsuHPI GGTGA 5 cut(s) 65, 229, 851, 1835, 2048
AsuII TTCGAA 1 cut(s) 1737
AvrII CCTAGG 1 cut(s) 827
BaeGI GKGCMC 2 cut(s) 108, 920
BalI TGGCCA 2 cut(s) 330, 453
BanI GGYRCC 2 cut(s) 831, 847
BanII GRGCYC 1 cut(s) 920
Bbv12I GWGCWC 2 cut(s) 1187, 1378
BccI CCATC 3 cut(s) 186, 320, 1814
BciT130I CCWGG 4 cut(s) 588, 913, 1203, 1644
BciVI GTATCC 1 cut(s) 364
BclI TGATCA 2 cut(s) 754, 1659
BcoDI GTCTC 3 cut(s) 129, 1120, 1827
BcuI ACTAGT 1 cut(s) 1748
BfaI CTAG 3 cut(s) 539, 828, 1749
BfrI CTTAAG 1 cut(s) 1217
BfuAI ACCTGC 1 cut(s) 2024
BfuI GTATCC 1 cut(s) 364
BlnI CCTAGG 1 cut(s) 827
Bme1390I CCNGG 4 cut(s) 588, 913, 1203, 1644
BmgT120I GGNCC 3 cut(s) 158, 916, 917
BmiI GGNNCC 5 cut(s) 118, 833, 849, 917, 918
BmrFI CCNGG 4 cut(s) 588, 913, 1203, 1644
BmsI GCATC 4 cut(s) 625, 708, 1222, 2064
BplI GAGNNNNNCTC 2 cut(s) 1302, 1334
BpmI CTGGAG 4 cut(s) 44, 887, 1551, 1590
Bpu10I CCTNAGC 1 cut(s) 1326
Bpu14I TTCGAA 1 cut(s) 1737
BpuEI CTTGAG 2 cut(s) 486, 1973
BsaI GGTCTC 1 cut(s) 1827
BsaJI CCNNGG 7 cut(s) 49, 827, 911, 912, 1201, 1643, 1931
BsaWI WCCGGW 2 cut(s) 1836, 2205
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 1524, 1554
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 1157
Bse118I RCCGGY 3 cut(s) 407, 1344, 1836
Bse1I ACTGG 3 cut(s) 27, 870, 2175
Bse3DI GCAATG 2 cut(s) 1238, 1268
BseAI TCCGGA 1 cut(s) 2205
BseBI CCWGG 4 cut(s) 588, 913, 1203, 1644
BseDI CCNNGG 7 cut(s) 49, 827, 911, 912, 1201, 1643, 1931
BseGI GGATG 5 cut(s) 640, 723, 1081, 1300, 1458
BseLI CCNNNNNNNGG 2 cut(s) 113, 1157
BseMI GCAATG 2 cut(s) 1238, 1268
BseMII CTCAG 6 cut(s) 72, 155, 305, 1259, 1289, 2152
BseNI ACTGG 3 cut(s) 27, 870, 2175
BseRI GAGGAG 1 cut(s) 133
BseSI GKGCMC 2 cut(s) 108, 920
BsgI GTGCAG 2 cut(s) 921, 2009
Bsh1285I CGRYCG 1 cut(s) 1777
BshFI GGCC 4 cut(s) 160, 330, 453, 918
BshNI GGYRCC 2 cut(s) 831, 847
BshTI ACCGGT 1 cut(s) 1836
BsiEI CGRYCG 1 cut(s) 1777
BsiHKAI GWGCWC 2 cut(s) 1187, 1378
BsiSI CCGG 5 cut(s) 408, 1132, 1345, 1837, 2206
BslFI GGGAC 1 cut(s) 1579
BslI CCNNNNNNNGG 2 cut(s) 113, 1157
BsmAI GTCTC 3 cut(s) 129, 1120, 1827
BsmFI GGGAC 1 cut(s) 1579
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 4 cut(s) 160, 330, 453, 918
Bso31I GGTCTC 1 cut(s) 1827
Bsp119I TTCGAA 1 cut(s) 1737
Bsp120I GGGCCC 1 cut(s) 916
Bsp1286I GDGCHC 4 cut(s) 108, 920, 1187, 1378
Bsp13I TCCGGA 1 cut(s) 2205
Bsp1407I TGTACA 1 cut(s) 550
Bsp19I CCATGG 1 cut(s) 1931
BspACI CCGC 4 cut(s) 90, 727, 760, 1073
BspANI GGCC 4 cut(s) 160, 330, 453, 918
BspCNI CTCAG 6 cut(s) 73, 156, 306, 1258, 1288, 2151
BspEI TCCGGA 1 cut(s) 2205
BspHI TCATGA 1 cut(s) 751
BspLI GGNNCC 5 cut(s) 118, 833, 849, 917, 918
BspMI ACCTGC 1 cut(s) 2024
BspPI GGATC 4 cut(s) 1338, 1433, 1877, 2097
BspQI GCTCTTC 2 cut(s) 480, 2034
BspT104I TTCGAA 1 cut(s) 1737
BspT107I GGYRCC 2 cut(s) 831, 847
BspTI CTTAAG 1 cut(s) 1217
BspTNI GGTCTC 1 cut(s) 1827
BsrDI GCAATG 2 cut(s) 1238, 1268
BsrFI RCCGGY 3 cut(s) 407, 1344, 1836
BsrGI TGTACA 1 cut(s) 550
BsrI ACTGG 3 cut(s) 27, 870, 2175
BssAI RCCGGY 3 cut(s) 407, 1344, 1836
BssECI CCNNGG 7 cut(s) 49, 827, 911, 912, 1201, 1643, 1931
BssT1I CCWWGG 3 cut(s) 49, 827, 1931
Bst2UI CCWGG 4 cut(s) 588, 913, 1203, 1644
Bst4CI ACNGT 4 cut(s) 791, 942, 1778, 1942
Bst6I CTCTTC 2 cut(s) 480, 2034
BstAFI CTTAAG 1 cut(s) 1217
BstAPI GCANNNNNTGC 1 cut(s) 1498
BstAUI TGTACA 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 1737
BstC8I GCNNGC 4 cut(s) 240, 582, 1700, 1767
BstDEI CTNAG 9 cut(s) 81, 164, 314, 680, 1245, 1275, 1326, 1808, 2138
BstDSI CCRYGG 1 cut(s) 1931
BstF5I GGATG 5 cut(s) 640, 723, 1081, 1300, 1458
BstHHI GCGC 2 cut(s) 1690, 2085
BstMAI GTCTC 3 cut(s) 129, 1120, 1827
BstMCI CGRYCG 1 cut(s) 1777
BstMWI GCNNNNNNNGC 3 cut(s) 801, 1067, 1498
BstNI CCWGG 4 cut(s) 588, 913, 1203, 1644
BstNSI RCATGY 4 cut(s) 472, 584, 781, 2083
BstSCI CCNGG 4 cut(s) 586, 911, 1201, 1642
BstSLI GKGCMC 2 cut(s) 108, 920
BstX2I RGATCY 2 cut(s) 1438, 1882
BstYI RGATCY 2 cut(s) 1438, 1882
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 4 cut(s) 160, 330, 453, 918
BtgI CCRYGG 1 cut(s) 1931
BtsCI GGATG 5 cut(s) 640, 723, 1081, 1300, 1458
BtsIMutI CAGTG 3 cut(s) 796, 938, 1634
BveI ACCTGC 1 cut(s) 2024
Cac8I GCNNGC 4 cut(s) 240, 582, 1700, 1767
CaiI CAGNNNCTG 2 cut(s) 530, 2068
CciI TCATGA 1 cut(s) 751
CfoI GCGC 2 cut(s) 1690, 2085
Cfr10I RCCGGY 3 cut(s) 407, 1344, 1836
Cfr13I GGNCC 3 cut(s) 158, 916, 917
CseI GACGC 1 cut(s) 2033
Csp6I GTAC 3 cut(s) 551, 736, 1224
CspAI ACCGGT 1 cut(s) 1836
CviQI GTAC 3 cut(s) 551, 736, 1224
DdeI CTNAG 9 cut(s) 81, 164, 314, 680, 1245, 1275, 1326, 1808, 2138
DrdI GACNNNNNNGTC 1 cut(s) 816
DseDI GACNNNNNNGTC 1 cut(s) 816
EaeI YGGCCR 2 cut(s) 328, 451
Eam1104I CTCTTC 2 cut(s) 480, 2034
EarI CTCTTC 2 cut(s) 480, 2034
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 3 cut(s) 49, 827, 1931
Eco24I GRGCYC 1 cut(s) 920
Eco31I GGTCTC 1 cut(s) 1827
Eco57I CTGAAG 4 cut(s) 1389, 1658, 1781, 1907
EcoO109I RGGNCCY 2 cut(s) 158, 916
EcoRII CCWGG 4 cut(s) 586, 911, 1201, 1642
EcoT14I CCWWGG 3 cut(s) 49, 827, 1931
EcoT22I ATGCAT 2 cut(s) 1215, 1563
EcoT38I GRGCYC 1 cut(s) 920
ErhI CCWWGG 3 cut(s) 49, 827, 1931
FaqI GGGAC 1 cut(s) 1579
FbaI TGATCA 2 cut(s) 754, 1659
FblI GTMKAC 2 cut(s) 808, 1944
FokI GGATG 5 cut(s) 647, 730, 1088, 1307, 1445
FriOI GRGCYC 1 cut(s) 920
FspBI CTAG 3 cut(s) 539, 828, 1749
FspI TGCGCA 1 cut(s) 1689
GlaI GCGC 2 cut(s) 1689, 2084
GsuI CTGGAG 4 cut(s) 44, 887, 1551, 1590
HaeIII GGCC 4 cut(s) 160, 330, 453, 918
HapII CCGG 5 cut(s) 408, 1132, 1345, 1837, 2206
HgaI GACGC 1 cut(s) 2033
HhaI GCGC 2 cut(s) 1690, 2085
Hin6I GCGC 2 cut(s) 1688, 2083
HinP1I GCGC 2 cut(s) 1688, 2083
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 1466
HinfI GANTC 6 cut(s) 86, 598, 907, 964, 1009, 1079
HpaII CCGG 5 cut(s) 408, 1132, 1345, 1837, 2206
HphI GGTGA 5 cut(s) 65, 229, 851, 1835, 2048
Hpy166II GTNNAC 4 cut(s) 139, 736, 809, 1945
Hpy8I GTNNAC 4 cut(s) 139, 736, 809, 1945
Hpy99I CGWCG 1 cut(s) 2049
HpyAV CCTTC 4 cut(s) 355, 1228, 1364, 2168
HpyCH4III ACNGT 4 cut(s) 791, 942, 1778, 1942
HpyCH4IV ACGT 1 cut(s) 1683
HpyF10VI GCNNNNNNNGC 3 cut(s) 801, 1067, 1498
HpyF3I CTNAG 9 cut(s) 81, 164, 314, 680, 1245, 1275, 1326, 1808, 2138
HpySE526I ACGT 1 cut(s) 1683
HspAI GCGC 2 cut(s) 1688, 2083
Kpn2I TCCGGA 1 cut(s) 2205
Ksp22I TGATCA 2 cut(s) 754, 1659
LguI GCTCTTC 2 cut(s) 480, 2034
LmnI GCTCC 7 cut(s) 51, 589, 775, 868, 1067, 1373, 2208
LweI GCATC 4 cut(s) 625, 708, 1222, 2064
MaeI CTAG 3 cut(s) 539, 828, 1749
MaeII ACGT 1 cut(s) 1683
MaeIII GTNAC 1 cut(s) 439
MboII GAAGA 6 cut(s) 24, 232, 497, 1499, 2051, 2236
MflI RGATCY 2 cut(s) 1438, 1882
MhlI GDGCHC 4 cut(s) 108, 920, 1187, 1378
MlsI TGGCCA 2 cut(s) 330, 453
MluNI TGGCCA 2 cut(s) 330, 453
MmeI TCCRAC 4 cut(s) 665, 744, 1045, 1941
Mox20I TGGCCA 2 cut(s) 330, 453
Mph1103I ATGCAT 2 cut(s) 1215, 1563
MroI TCCGGA 1 cut(s) 2205
MroXI GAANNNNTTC 2 cut(s) 1008, 1363
MscI TGGCCA 2 cut(s) 330, 453
MseI TTAA 6 cut(s) 293, 492, 957, 1218, 1542, 1709
MslI CAYNNNNRTG 2 cut(s) 186, 1818
Msp20I TGGCCA 2 cut(s) 330, 453
MspA1I CMGCKG 4 cut(s) 530, 727, 760, 1073
MspCI CTTAAG 1 cut(s) 1217
MspI CCGG 5 cut(s) 408, 1132, 1345, 1837, 2206
MspR9I CCNGG 4 cut(s) 588, 913, 1203, 1644
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 4 cut(s) 588, 913, 1203, 1644
MwoI GCNNNNNNNGC 3 cut(s) 801, 1067, 1498
NcoI CCATGG 1 cut(s) 1931
NlaIV GGNNCC 5 cut(s) 118, 833, 849, 917, 918
NmuCI GTSAC 1 cut(s) 439
NsbI TGCGCA 1 cut(s) 1689
NsiI ATGCAT 2 cut(s) 1215, 1563
NspI RCATGY 4 cut(s) 472, 584, 781, 2083
NspV TTCGAA 1 cut(s) 1737
PaeI GCATGC 1 cut(s) 584
PagI TCATGA 1 cut(s) 751
PaqCI CACCTGC 1 cut(s) 2024
PasI CCCWGGG 1 cut(s) 912
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 2 cut(s) 480, 2034
PctI GAATGC 1 cut(s) 70
PdmI GAANNNNTTC 2 cut(s) 1008, 1363
PfeI GAWTC 6 cut(s) 86, 598, 907, 964, 1009, 1079
PflMI CCANNNNNTGG 1 cut(s) 1157
PinAI ACCGGT 1 cut(s) 1836
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 4 cut(s) 586, 911, 1201, 1642
PspGI CCWGG 4 cut(s) 586, 911, 1201, 1642
PspN4I GGNNCC 5 cut(s) 118, 833, 849, 917, 918
PspOMI GGGCCC 1 cut(s) 916
PspPI GGNCC 3 cut(s) 158, 916, 917
PstNI CAGNNNCTG 2 cut(s) 530, 2068
PsuI RGATCY 2 cut(s) 1438, 1882
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 552, 737, 1225
RsaNI GTAC 3 cut(s) 551, 736, 1224
RseI CAYNNNNRTG 2 cut(s) 186, 1818
SapI GCTCTTC 2 cut(s) 480, 2034
SaqAI TTAA 6 cut(s) 293, 492, 957, 1218, 1542, 1709
Sau96I GGNCC 3 cut(s) 158, 916, 917
ScrFI CCNGG 4 cut(s) 588, 913, 1203, 1644
SduI GDGCHC 4 cut(s) 108, 920, 1187, 1378
SfaNI GCATC 4 cut(s) 625, 708, 1222, 2064
SfuI TTCGAA 1 cut(s) 1737
SmiMI CAYNNNNRTG 2 cut(s) 186, 1818
SmlI CTYRAG 3 cut(s) 501, 1217, 1952
SmoI CTYRAG 3 cut(s) 501, 1217, 1952
SpeI ACTAGT 1 cut(s) 1748
SphI GCATGC 1 cut(s) 584
SsiI CCGC 4 cut(s) 90, 727, 760, 1073
SspI AATATT 3 cut(s) 479, 710, 1546
SspMI CTAG 3 cut(s) 539, 828, 1749
StyD4I CCNGG 4 cut(s) 586, 911, 1201, 1642
StyI CCWWGG 3 cut(s) 49, 827, 1931
TaaI ACNGT 4 cut(s) 791, 942, 1778, 1942
TaiI ACGT 1 cut(s) 1686
TaqI TCGA 2 cut(s) 1404, 1737
TatI WGTACW 3 cut(s) 550, 735, 1223
TfiI GAWTC 6 cut(s) 86, 598, 907, 964, 1009, 1079
Tru1I TTAA 6 cut(s) 293, 492, 957, 1218, 1542, 1709
Tru9I TTAA 6 cut(s) 293, 492, 957, 1218, 1542, 1709
TscAI CASTG 3 cut(s) 796, 945, 1641
TseFI GTSAC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 8 cut(s) 48, 740, 956, 1046, 1092, 1311, 1499, 2150
TspRI CASTG 3 cut(s) 796, 945, 1641
Van91I CCANNNNNTGG 1 cut(s) 1157
Vha464I CTTAAG 1 cut(s) 1217
XapI RAATTY 6 cut(s) 35, 225, 1052, 1361, 1503, 1654
XceI RCATGY 4 cut(s) 472, 584, 781, 2083
XcmI CCANNNNNNNNNTGG 2 cut(s) 185, 1886
XmaJI CCTAGG 1 cut(s) 827
XmiI GTMKAC 2 cut(s) 808, 1944
XmnI GAANNNNTTC 2 cut(s) 1008, 1363
XspI CTAG 3 cut(s) 539, 828, 1749
Zsp2I ATGCAT 2 cut(s) 1215, 1563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.