Rw2G018590

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001707
Physical Location & Seq
Reverse (-)
24163703 .. 24165135
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018590.1

Sequence Viewer

Length: 639 bp
ATGGTTGTTTCCTCACTAAAGCCTGGGATAGTGAACTTGATAAAGAACACAAATGGCAACCACGTTGCGCAGCGCTGCTTGCAGTATTTAACACCTGAATATCGTGAGTTTCTTTTTGAAGCTGCAACTAGTAATTGTGTTGAGCTTGCAACCGACCGTCATGGCTGTTGTGTGCTTCAAAAATGTCTTAGTCATTCTGATGGTGAGCAAAGAGACCGATTGATCTGCAAGATCACTTCCAATGCACTAATCCTTTCCCAAGATCCATTTGGGAACTATGTCGTGCAATTCGTCTTTGAGCTTCAGCTTCCGTGGGCGACAGTAGACATTCTTGAGCAGTTGGAAGGTAATTATGGGGATTTGTCTGTGCAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGTCGTGCTCGCATTATTCAGGAACTGATAGGGAATATGCGATTGGACCAAATCATGCAAGACCCTTATGGCAATTATGTTATCCAAGCAGCACTCAGTCAATCAAAGGGAACACTTCATTCTAAACTGGTAGAAGTAATAAAACCTCATATGCCAGTTCTCCGGACCAGTCCCTATGGGAAGAAAATCCTCTCTACCAATATTTTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

212

Amino Acids

23.93

Weight (kDa)

8.48

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 10 - 40 2.2e-06 Pumilio-family RNA binding repeat
PUF PF00806 45 - 79 2.9e-16 Pumilio-family RNA binding repeat
PUF PF00806 118 - 151 3.1e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 404
Acc16I TGCGCA 1 cut(s) 69
Acc36I ACCTGC 1 cut(s) 404
AccI GTMKAC 1 cut(s) 324
AccIII TCCGGA 1 cut(s) 585
AclWI GGATC 1 cut(s) 257
AcuI CTGAAG 1 cut(s) 287
AfeI AGCGCT 1 cut(s) 74
AgsI TTSAA 4 cut(s) 119, 179, 395, 631
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 1 cut(s) 22
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 4 cut(s) 122, 145, 301, 307
AluI AGCT 4 cut(s) 122, 145, 301, 307
Alw21I GWGCWC 1 cut(s) 433
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 448
Aor13HI TCCGGA 1 cut(s) 585
Aor51HI AGCGCT 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 70, 75, 122, 512
AspLEI GCGC 2 cut(s) 70, 75
AspS9I GGNCC 2 cut(s) 469, 588
AsuHPI GGTGA 2 cut(s) 215, 428
AvaII GGWCC 2 cut(s) 469, 588
Bbv12I GWGCWC 1 cut(s) 433
BbvI GCAGC 4 cut(s) 62, 82, 109, 524
BccI CCATC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 207
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 4 cut(s) 71, 76, 123, 513
BlsI GCNGC 4 cut(s) 72, 77, 124, 514
Bme1390I CCNGG 1 cut(s) 24
Bme18I GGWCC 2 cut(s) 469, 588
BmgT120I GGNCC 2 cut(s) 469, 588
BmrFI CCNGG 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 353
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 23, 311
BsaWI WCCGGW 1 cut(s) 585
Bse1I ACTGG 3 cut(s) 555, 578, 591
BseAI TCCGGA 1 cut(s) 585
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 2 cut(s) 23, 311
BseMII CTCAG 1 cut(s) 532
BseNI ACTGG 3 cut(s) 555, 578, 591
BseXI GCAGC 4 cut(s) 62, 82, 109, 524
BsgI GTGCAG 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 157
BsiEI CGRYCG 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 433
BsiSI CCGG 1 cut(s) 586
BslFI GGGAC 1 cut(s) 579
BsmAI GTCTC 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 579
Bso31I GGTCTC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 433
Bsp13I TCCGGA 1 cut(s) 585
Bsp143I GATC 3 cut(s) 222, 231, 262
BspCNI CTCAG 1 cut(s) 531
BspEI TCCGGA 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 207
BsrI ACTGG 3 cut(s) 555, 578, 591
BssECI CCNNGG 2 cut(s) 23, 311
BssMI GATC 3 cut(s) 222, 231, 262
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 158, 322
Bst6I CTCTTC 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 80, 147, 433
BstDEI CTNAG 2 cut(s) 188, 518
BstDSI CCRYGG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 70, 75
BstKTI GATC 3 cut(s) 225, 234, 265
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 3 cut(s) 222, 231, 262
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstV1I GCAGC 4 cut(s) 62, 82, 109, 524
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 311
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 3 cut(s) 80, 147, 433
CaiI CAGNNNCTG 1 cut(s) 448
CfoI GCGC 2 cut(s) 70, 75
Cfr13I GGNCC 2 cut(s) 469, 588
CseI GACGC 1 cut(s) 413
CviAII CATG 2 cut(s) 161, 478
CviJI RGCY 7 cut(s) 22, 122, 145, 165, 301, 307, 402
CviKI_1 RGCY 7 cut(s) 22, 122, 145, 165, 301, 307, 402
DdeI CTNAG 2 cut(s) 188, 518
DpnI GATC 3 cut(s) 224, 233, 264
DpnII GATC 3 cut(s) 222, 231, 262
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 469, 588
Eco47III AGCGCT 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 22
FaeI CATG 2 cut(s) 164, 481
FaqI GGGAC 1 cut(s) 579
FatI CATG 2 cut(s) 160, 477
FauNDI CATATG 1 cut(s) 573
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 71, 76, 123, 513
Fsp4HI GCNGC 4 cut(s) 71, 76, 123, 513
FspBI CTAG 1 cut(s) 129
FspI TGCGCA 1 cut(s) 69
GlaI GCGC 2 cut(s) 69, 74
GluI GCNGC 4 cut(s) 71, 76, 123, 513
HaeII RGCGCY 1 cut(s) 76
HapII CCGG 1 cut(s) 586
HgaI GACGC 1 cut(s) 413
HhaI GCGC 2 cut(s) 70, 75
Hin1II CATG 2 cut(s) 164, 481
Hin6I GCGC 2 cut(s) 68, 73
HinP1I GCGC 2 cut(s) 68, 73
HpaII CCGG 1 cut(s) 586
HphI GGTGA 2 cut(s) 215, 428
Hpy166II GTNNAC 2 cut(s) 34, 325
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 4 cut(s) 104, 332, 443, 586
Hpy8I GTNNAC 2 cut(s) 34, 325
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 2 cut(s) 158, 322
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 8 cut(s) 82, 125, 149, 228, 245, 286, 370, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 2 cut(s) 188, 518
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 2 cut(s) 164, 481
HspAI GCGC 2 cut(s) 68, 73
Kpn2I TCCGGA 1 cut(s) 585
Kzo9I GATC 3 cut(s) 222, 231, 262
LguI GCTCTTC 1 cut(s) 414
Lsp1109I GCAGC 4 cut(s) 62, 82, 109, 524
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 63
MalI GATC 3 cut(s) 224, 233, 264
MboI GATC 3 cut(s) 222, 231, 262
MboII GAAGA 2 cut(s) 431, 616
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 433
MluCI AATT 4 cut(s) 133, 287, 349, 496
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 3 cut(s) 22, 579, 623
MroI TCCGGA 1 cut(s) 585
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 1 cut(s) 586
MspR9I CCNGG 1 cut(s) 24
MteI GCGCNGCGC 1 cut(s) 71
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 79
NdeI CATATG 1 cut(s) 573
NdeII GATC 3 cut(s) 222, 231, 262
NlaIII CATG 2 cut(s) 164, 481
NsbI TGCGCA 1 cut(s) 69
PaqCI CACCTGC 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PkrI GCNGC 4 cut(s) 72, 77, 124, 514
Psp6I CCWGG 1 cut(s) 22
PspGI CCWGG 1 cut(s) 22
PspPI GGNCC 2 cut(s) 469, 588
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 4 cut(s) 71, 76, 123, 513
Sau3AI GATC 3 cut(s) 222, 231, 262
Sau96I GGNCC 2 cut(s) 469, 588
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 433
SetI ASST 9 cut(s) 66, 97, 124, 147, 303, 309, 349, 418, 571
SinI GGWCC 2 cut(s) 469, 588
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 4 cut(s) 133, 287, 349, 496
SspI AATATT 1 cut(s) 625
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 158, 322
TaiI ACGT 1 cut(s) 66
TaqII GACCGA 1 cut(s) 231
TasI AATT 4 cut(s) 133, 287, 349, 496
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TseI GCWGC 4 cut(s) 70, 75, 122, 512
TspDTI ATGAA 1 cut(s) 530
TspGWI ACGGA 1 cut(s) 300
VpaK11BI GGWCC 2 cut(s) 469, 588
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.