Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001707

Physical Location & Seq

Genomic Coordinates Forward (+)

242767 .. 247670

Gene Structure 4904 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1002800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGGAAGATGGAAGAACTGAACTAGAGTTTGATGAATTTGAAAAGCTTCTGGGTGAGATACCAAATGCTACTTCTGGGAACACACATTCTGAGGAATCTGGAACAAAGAATGCGTCATTGTCTCCCATCTGTGTGAACTCCTGCAAAGGACCATTGAGTGACAAACTCCATAACAATGGGAGGCTGGATGAGGGGAAGAACTCGGCAAAGAAAATTCAGAAATCACCTGTGAAAAGGGTTCAACGAGAGGTAACAAATCTGCCCGATGATCAATCCTTAACGTCTGCACTCGCAGGACTGAGCTTTGATGGCGCAGTGAATATGGAAGCTGGGAGTCATGTCGAAAATTGTAAATCCTTGCAGAACTACTTTTCAATGGATGGACAAATCCCAAACAGCTTAAAGATGCAGGTTTCAAGCATGGATACGCCGGTCATGGTTGTTCCCTCTTTCCAAGCACCAAATAATATACCTTGTGGATTTTATGAGTTTGATGTGACAAACTCAGGCCAAGAAAGTTCAAACATGTCAAAGTTCAATTCTGAAGAGCTTAAGAAACCGCAGGCTGCCTATTCACAACCTGTTGAAAATTTATCTCCTGGTGTTCCTCTAGCTCATGCAGTGGAAGGTTTTCAGTTCCTCTCTAATGTGCCTGCTCCTGGTCTGCAGTTTCCTCTAACTTCTGATCATCAGCAGCAATTCTTTCTTGATGCACAATCTCGTATTCCTTACCAACATTCGCAACAGAATCAAATTAGCTGGAGAAACATGGAAGAGGAGCAATACCATAGGATGCAGGAGCAATATGTATACTTGCAGCAGGTTCATGATCAGCGGTTGGAAGCTCAGCATCTGGTTCAAGGGAATGGGATTATCGCAAGCAGGCTCACGCGTCCAAACCCAAGGCACCCCCATTTTGAGGTGCCAATCTCTCATCGGCTTGAACAATCTAATCAAGAACGAATATGGAACAATTATGTGGTTCCCAGGGGTCCACACGAATCGAATCCTGCTCTTTCGTACACAGATTTTAACAGGGTTCAAGTTCTTGACAAGTTGGGAAAGCAGAGTTTTCCTGAGAAGATTCTAACAAGATCACAGGGAATGAACACACTAAAAGCTGTGAAGTTTGGTGCTGTTGGAACAGATGAATCACTTACTCATATTAGCCAGAATGGCAAAGTTGTCTCGAATGGTCATTTCCGGCATGCCTTATCTACTCGAAATACCGGGTGCTTTCAATTGGATAGTTTGAACACATGGGGTATGTTCCCTAGTTTAATGCATCTTAAGAATACAGATATGAAGGTGCTACCTCAGAAATATACTGATCTGAAGACATTGCCTCAGAAATATAACTCCATGGATGAAATTACTGGAAGAATTTATCTAATGGCTAAGGATCAGCATGGTTGCCGCTTCTTACAAAGAAAGTTTTCTGAAGGCACCCCAAAAGATGTCGAAAATATTTTCTTTGAGATCATTGATCACATTGTTGAGCTCATGACAGACCCTTTTGGGAATTACCTCATCCAGAAGCTGCTTGAAGTGTGTGATGAGGATCAGCAAATGCAAATTCTTCATTCAATCACTAAAAAGCCAGGGGAACTTGTTAGGATTTCATGTGATATGCATGGGACTCGAGCTGTTCAAAAGGTTATTGAAACCCTCAAAACTCCAGAGCAGTTTTCCATGGTTGTTTCATCGCTAAAGCCAGGGATAGTGATGTTGATAAAGAACACAAATGGAAATCACGTTGCACAGCGCTGCTTACAGTATTTAACACGTGAATATCGTGAGTTTCTTTTCGTAGCTGCAACTACTAACTGTGTAGAGCTTGCAACTGACCGCCATGGTTGTTGTGTGCTACAAAAATGCCTTAGCCATTCGGATGCTGAACAAAGAAACCGATTAATCTGCAAGATTACTTCAAATGCACTAATCCTTTCTCAAGATCCATTTGGGAACTATGTTGTACAATTTGTCTTTGAACTTCAGCTTCCGTGGGCAACTTTAGATATTCTTGACCAGTTGGAGGGTAATTATGGGGATTTGTCTGTGCAGAAGTATAGCAGTAATGTGGTTGAGAAAAGCCTGAAATATGCATGTGAAGAGCGGCGCATACGCATTATCCAGGAGCTGATAGAAAATCCGCGCTTGGATCAAGTCATGCAAGACCCTTATGGAAATTATGTTGTCCAAGCAGCGCTTAGTCAGTCAAAAGTAAGGAATCCTTCATTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUF

Protein Analysis

748

Amino Acids

84.68

Weight (kDa)

6.2

Isoelectric Point (pI)

39.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	461 - 495	3.6e-10	Pumilio-family RNA binding repeat
PUF	PF00806	497 - 530	6.4e-12	Pumilio-family RNA binding repeat
PUF	PF00806	609 - 643	8.6e-15	Pumilio-family RNA binding repeat
PUF	PF00806	682 - 715	6.5e-06	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001707)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56510 AT5G56510 AT5G56510 AT5G56510 AT5G56510 AT5G56510
fragaria_vesca	FvH4_3g45790 FvH4_3g45790 FvH4_3g45790 FvH4_3g45790
malus_domestica	MD03G1003000.v1.1 MD11G1002800.v1.1
prunus_persica	Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1 Prupe.6G002600_v2.0.a1
pyrus_communis	pycom03g00290 pycom03g00300 pycom11g00260
rosa_chinensis	RchiOBHm_Chr3g0468621 RchiOBHm_Chr5g0083871
rosa_laevigata	RLG00000037060
rosa_multiflora	Rmu_sc0001152.1_g000020 Rmu_sc0012873.1_g000001 Rmu_sc0014979.1_g000001 Rmu_sc0024007.1_g000007 Rmu_sc0026864.1_g000002
rosa_roxburghii	Rroxscaffold_1G00000720 Rroxscaffold_1G00026610 Rroxscaffold_3G00221790 Rroxscaffold_6G00406920 Rroxscaffold_6G00406930 Rroxscaffold_7G00203860
rosa_rugosa	Rorug05G0486900.1 Rorug05G0487000
rosa_samantha	Rh1AG075100 Rh1CG090400 Rh3AG144600 Rh5AG539900 Rh5BG565100 Rh5CG586900
rosa_wichuraiana	Rw2G018590 Rw5G048100 Rw5G050200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	400, 811
AccB1I	GGYRCC	3 cut(s)	906, 922, 1445
AccBSI	CCGCTC	1 cut(s)	2116
AccI	GTMKAC	1 cut(s)	810
AccII	CGCG	2 cut(s)	892, 2155
AciI	CCGC	6 cut(s)	560, 835, 1417, 1849, 2116, 2153
AclWI	GGATC	4 cut(s)	1410, 1569, 1949, 2169
AcsI	RAATTY	5 cut(s)	35, 213, 589, 1383, 1575
AcuI	CTGAAG	4 cut(s)	564, 1355, 1461, 1979
AcvI	CACGTG	1 cut(s)	1787
AfaI	GTAC	2 cut(s)	1022, 1977
AfeI	AGCGCT	2 cut(s)	1766, 2208
AfiI	CCNNNNNNNGG	3 cut(s)	659, 919, 2035
AflII	CTTAAG	2 cut(s)	551, 1289
AflIII	ACRYGT	3 cut(s)	525, 890, 1784
AjnI	CCWGG	6 cut(s)	598, 658, 986, 1600, 1714, 2133
AjuI	GAANNNNNNNTTGG	2 cut(s)	55, 87
Alw21I	GWGCWC	1 cut(s)	1503
Alw26I	GTCTC	2 cut(s)	126, 1192
AlwI	GGATC	4 cut(s)	1410, 1569, 1949, 2169
AlwNI	CAGNNNCTG	3 cut(s)	853, 1540, 2140
Ama87I	CYCGRG	1 cut(s)	1641
Aor51HI	AGCGCT	2 cut(s)	1766, 2208
AoxI	GGCC	1 cut(s)	508
ApeKI	GCWGC	7 cut(s)	566, 694, 817, 1540, 1767, 1814, 2204
ApoI	RAATTY	5 cut(s)	35, 213, 589, 1383, 1575
ArsI	GACNNNNNNTTYG	2 cut(s)	98, 130
AseI	ATTAAT	1 cut(s)	1913
Asp700I	GAANNNNTTC	3 cut(s)	45, 630, 1435
AspLEI	GCGC	5 cut(s)	314, 1767, 2121, 2157, 2209
AspS9I	GGNCC	2 cut(s)	149, 992
AsuC2I	CCSGG	1 cut(s)	1231
AsuHPI	GGTGA	2 cut(s)	65, 216
AvaI	CYCGRG	1 cut(s)	1641
AvaII	GGWCC	2 cut(s)	149, 992
BaeI	ACNNNNGTAYC	2 cut(s)	417, 450
BanI	GGYRCC	3 cut(s)	906, 922, 1445
BanII	GRGCYC	1 cut(s)	1503
BbrPI	CACGTG	1 cut(s)	1787
BbsI	GAAGAC	1 cut(s)	1343
Bbv12I	GWGCWC	1 cut(s)	1503
BbvI	GCAGC	7 cut(s)	553, 706, 829, 1527, 1754, 1801, 2216
BccI	CCATC	4 cut(s)	2, 134, 302, 374
BcgI	CGANNNNNNTGC	4 cut(s)	1622, 1656, 1899, 1933
BciT130I	CCWGG	6 cut(s)	600, 660, 988, 1602, 1716, 2135
BciVI	GTATCC	1 cut(s)	418
BclI	TGATCA	4 cut(s)	268, 685, 829, 1486
BcnI	CCSGG	1 cut(s)	1231
BcoDI	GTCTC	2 cut(s)	126, 1192
BfaI	CTAG	3 cut(s)	23, 611, 1275
BfmI	CTRYAG	1 cut(s)	665
BfoI	RGCGCY	2 cut(s)	1768, 2210
BfrI	CTTAAG	2 cut(s)	551, 1289
BfuAI	ACCTGC	2 cut(s)	400, 811
BfuI	GTATCC	1 cut(s)	418
BglI	GCCNNNNNGGC	1 cut(s)	1176
BisI	GCNGC	9 cut(s)	567, 695, 818, 1417, 1541, 1768, 1815, 2117, 2205
BlpI	GCTNAGC	1 cut(s)	846
BlsI	GCNGC	9 cut(s)	568, 696, 819, 1418, 1542, 1769, 1816, 2118, 2206
Bme1390I	CCNGG	7 cut(s)	600, 660, 988, 1231, 1602, 1716, 2135
Bme18I	GGWCC	2 cut(s)	149, 992
BmeT110I	CYCGRG	1 cut(s)	1641
BmgT120I	GGNCC	2 cut(s)	149, 992
BmiI	GGNNCC	5 cut(s)	908, 924, 984, 993, 1447
BmrFI	CCNGG	7 cut(s)	600, 660, 988, 1231, 1602, 1716, 2135
BmsI	GCATC	6 cut(s)	396, 700, 783, 859, 1294, 1882
BpiI	GAAGAC	1 cut(s)	1343
BpmI	CTGGAG	2 cut(s)	781, 1662
Bpu10I	CCTNAGC	2 cut(s)	1398, 1880
Bpu1102I	GCTNAGC	1 cut(s)	846
BpuEI	CTTGAG	1 cut(s)	1935
BpuMI	CCSGG	1 cut(s)	1231
BsaAI	YACGTR	1 cut(s)	1787
BsaBI	GATNNNNATC	1 cut(s)	1560
BsaJI	CCNNGG	9 cut(s)	902, 986, 987, 1362, 1601, 1692, 1715, 1852, 2003
Bsc4I	CCNNNNNNNGG	3 cut(s)	659, 919, 2035
Bse118I	RCCGGY	1 cut(s)	430
Bse1I	ACTGG	2 cut(s)	1381, 2029
Bse3DI	GCAATG	1 cut(s)	1340
Bse8I	GATNNNNATC	1 cut(s)	1560
BseBI	CCWGG	6 cut(s)	600, 660, 988, 1602, 1716, 2135
BseDI	CCNNGG	9 cut(s)	902, 986, 987, 1362, 1601, 1692, 1715, 1852, 2003
BseGI	GGATG	6 cut(s)	193, 385, 798, 1372, 1530, 1897
BseJI	GATNNNNATC	1 cut(s)	1560
BseLI	CCNNNNNNNGG	3 cut(s)	659, 919, 2035
BseMI	GCAATG	1 cut(s)	1340
BseMII	CTCAG	7 cut(s)	81, 290, 519, 860, 1068, 1331, 1361
BseNI	ACTGG	2 cut(s)	1381, 2029
BseRI	GAGGAG	1 cut(s)	791
BseXI	GCAGC	7 cut(s)	553, 706, 829, 1527, 1754, 1801, 2216
BseYI	CCCAGC	1 cut(s)	329
BsgI	GTGCAG	2 cut(s)	270, 2081
Bsh1236I	CGCG	2 cut(s)	892, 2155
BshFI	GGCC	1 cut(s)	510
BshNI	GGYRCC	3 cut(s)	906, 922, 1445
BsiHKAI	GWGCWC	1 cut(s)	1503
BsiHKCI	CYCGRG	1 cut(s)	1641
BsiSI	CCGG	3 cut(s)	431, 1204, 1230
BslFI	GGGAC	1 cut(s)	1651
BslI	CCNNNNNNNGG	3 cut(s)	659, 919, 2035
BsmAI	GTCTC	2 cut(s)	126, 1192
BsmFI	GGGAC	1 cut(s)	1651
BsmI	GAATGC	1 cut(s)	115
BsnI	GGCC	1 cut(s)	510
BsoBI	CYCGRG	1 cut(s)	1641
Bsp1286I	GDGCHC	1 cut(s)	1503
Bsp1407I	TGTACA	1 cut(s)	1975
Bsp1720I	GCTNAGC	1 cut(s)	846
Bsp19I	CCATGG	3 cut(s)	1362, 1692, 1852
BspACI	CCGC	6 cut(s)	560, 835, 1417, 1849, 2116, 2153
BspANI	GGCC	1 cut(s)	510
BspCNI	CTCAG	7 cut(s)	82, 291, 518, 859, 1069, 1330, 1360
BspFNI	CGCG	2 cut(s)	892, 2155
BspHI	TCATGA	2 cut(s)	826, 1503
BspLI	GGNNCC	5 cut(s)	908, 924, 984, 993, 1447
BspMAI	CTGCAG	1 cut(s)	669
BspMI	ACCTGC	2 cut(s)	400, 811
BspPI	GGATC	4 cut(s)	1410, 1569, 1949, 2169
BspQI	GCTCTTC	2 cut(s)	540, 2106
BspT107I	GGYRCC	3 cut(s)	906, 922, 1445
BspTI	CTTAAG	2 cut(s)	551, 1289
BsrBI	CCGCTC	1 cut(s)	2116
BsrDI	GCAATG	1 cut(s)	1340
BsrFI	RCCGGY	1 cut(s)	430
BsrGI	TGTACA	1 cut(s)	1975
BsrI	ACTGG	2 cut(s)	1381, 2029
BssAI	RCCGGY	1 cut(s)	430
BssECI	CCNNGG	9 cut(s)	902, 986, 987, 1362, 1601, 1692, 1715, 1852, 2003
BssNAI	GTATAC	1 cut(s)	811
BssT1I	CCWWGG	4 cut(s)	902, 1362, 1692, 1852
Bst1107I	GTATAC	1 cut(s)	811
Bst2UI	CCWGG	6 cut(s)	600, 660, 988, 1602, 1716, 2135
Bst4CI	ACNGT	2 cut(s)	1776, 1829
Bst6I	CTCTTC	3 cut(s)	540, 768, 2106
BstAFI	CTTAAG	2 cut(s)	551, 1289
BstAUI	TGTACA	1 cut(s)	1975
BstBAI	YACGTR	1 cut(s)	1787
BstC8I	GCNNGC	6 cut(s)	564, 654, 880, 884, 1209, 1839
BstDSI	CCRYGG	4 cut(s)	1362, 1692, 1852, 2003
BstF5I	GGATG	6 cut(s)	193, 385, 798, 1372, 1530, 1897
BstFNI	CGCG	2 cut(s)	892, 2155
BstH2I	RGCGCY	2 cut(s)	1768, 2210
BstHHI	GCGC	5 cut(s)	314, 1767, 2121, 2157, 2209
BstMAI	GTCTC	2 cut(s)	126, 1192
BstMWI	GCNNNNNNNGC	2 cut(s)	309, 1176
BstNI	CCWGG	6 cut(s)	600, 660, 988, 1602, 1716, 2135
BstNSI	RCATGY	3 cut(s)	529, 1211, 2109
BstSCI	CCNGG	7 cut(s)	598, 658, 986, 1229, 1600, 1714, 2133
BstSFI	CTRYAG	1 cut(s)	665
BstUI	CGCG	2 cut(s)	892, 2155
BstV1I	GCAGC	7 cut(s)	553, 706, 829, 1527, 1754, 1801, 2216
BstV2I	GAAGAC	1 cut(s)	1343
BstX2I	RGATCY	1 cut(s)	1954
BstXI	CCANNNNNNTGG	1 cut(s)	176
BstYI	RGATCY	1 cut(s)	1954
BstZ17I	GTATAC	1 cut(s)	811
BsuI	GTATCC	1 cut(s)	418
BsuRI	GGCC	1 cut(s)	510
BtgI	CCRYGG	4 cut(s)	1362, 1692, 1852, 2003
BtgZI	GCGATG	1 cut(s)	1689
BtsCI	GGATG	6 cut(s)	193, 385, 798, 1372, 1530, 1897
BtsI	GCAGTG	2 cut(s)	321, 627
BtsIMutI	CAGTG	2 cut(s)	321, 627
BveI	ACCTGC	2 cut(s)	400, 811
Cac8I	GCNNGC	6 cut(s)	564, 654, 880, 884, 1209, 1839
CaiI	CAGNNNCTG	3 cut(s)	853, 1540, 2140
CciI	TCATGA	2 cut(s)	826, 1503
CfoI	GCGC	5 cut(s)	314, 1767, 2121, 2157, 2209
Cfr10I	RCCGGY	1 cut(s)	430
Cfr13I	GGNCC	2 cut(s)	149, 992
CseI	GACGC	2 cut(s)	102, 881
Csp6I	GTAC	2 cut(s)	1021, 1976
CviQI	GTAC	2 cut(s)	1021, 1976
Eam1104I	CTCTTC	3 cut(s)	540, 768, 2106
EarI	CTCTTC	3 cut(s)	540, 768, 2106
Ecl136II	GAGCTC	1 cut(s)	1501
Eco130I	CCWWGG	4 cut(s)	902, 1362, 1692, 1852
Eco24I	GRGCYC	1 cut(s)	1503
Eco47I	GGWCC	2 cut(s)	149, 992
Eco47III	AGCGCT	2 cut(s)	1766, 2208
Eco53kI	GAGCTC	1 cut(s)	1501
Eco57I	CTGAAG	4 cut(s)	564, 1355, 1461, 1979
Eco72I	CACGTG	1 cut(s)	1787
Eco88I	CYCGRG	1 cut(s)	1641
EcoICRI	GAGCTC	1 cut(s)	1501
EcoRII	CCWGG	6 cut(s)	598, 658, 986, 1600, 1714, 2133
EcoT14I	CCWWGG	4 cut(s)	902, 1362, 1692, 1852
EcoT22I	ATGCAT	3 cut(s)	1287, 1635, 2107
EcoT38I	GRGCYC	1 cut(s)	1503
ErhI	CCWWGG	4 cut(s)	902, 1362, 1692, 1852
FalI	AAGNNNNNCTT	3 cut(s)	2193, 2225, 2218
FaqI	GGGAC	1 cut(s)	1651
FbaI	TGATCA	4 cut(s)	268, 685, 829, 1486
FblI	GTMKAC	1 cut(s)	810
Fnu4HI	GCNGC	9 cut(s)	567, 695, 818, 1417, 1541, 1768, 1815, 2117, 2205
FokI	GGATG	6 cut(s)	200, 392, 805, 1379, 1517, 1904
FriOI	GRGCYC	1 cut(s)	1503
Fsp4HI	GCNGC	9 cut(s)	567, 695, 818, 1417, 1541, 1768, 1815, 2117, 2205
FspBI	CTAG	3 cut(s)	23, 611, 1275
GlaI	GCGC	5 cut(s)	313, 1766, 2120, 2156, 2208
GluI	GCNGC	9 cut(s)	567, 695, 818, 1417, 1541, 1768, 1815, 2117, 2205
GsaI	CCCAGC	1 cut(s)	333
GsuI	CTGGAG	2 cut(s)	781, 1662
HaeII	RGCGCY	2 cut(s)	1768, 2210
HaeIII	GGCC	1 cut(s)	510
HapII	CCGG	3 cut(s)	431, 1204, 1230
HgaI	GACGC	2 cut(s)	102, 881
HhaI	GCGC	5 cut(s)	314, 1767, 2121, 2157, 2209
Hin6I	GCGC	5 cut(s)	312, 1765, 2119, 2155, 2207
HinP1I	GCGC	5 cut(s)	312, 1765, 2119, 2155, 2207
HindIII	AAGCTT	1 cut(s)	44
HinfI	GANTC	9 cut(s)	95, 334, 748, 1001, 1006, 1084, 1151, 1639, 2230
HpaII	CCGG	3 cut(s)	431, 1204, 1230
HphI	GGTGA	2 cut(s)	65, 216
Hpy166II	GTNNAC	4 cut(s)	136, 811, 995, 1023
Hpy188I	TCNGA	9 cut(s)	91, 219, 544, 685, 1320, 1335, 1350, 1441, 1891
Hpy8I	GTNNAC	4 cut(s)	136, 811, 995, 1023
HpyAV	CCTTC	4 cut(s)	620, 1300, 1436, 2244
HpyCH4III	ACNGT	2 cut(s)	1776, 1829
HpyCH4IV	ACGT	3 cut(s)	281, 1755, 1786
HpyF10VI	GCNNNNNNNGC	2 cut(s)	309, 1176
HpySE526I	ACGT	3 cut(s)	281, 1755, 1786
HspAI	GCGC	5 cut(s)	312, 1765, 2119, 2155, 2207
Ksp22I	TGATCA	4 cut(s)	268, 685, 829, 1486
LguI	GCTCTTC	2 cut(s)	540, 2106
LmnI	GCTCC	4 cut(s)	661, 778, 799, 2137
Lsp1109I	GCAGC	7 cut(s)	553, 706, 829, 1527, 1754, 1801, 2216
LweI	GCATC	6 cut(s)	396, 700, 783, 859, 1294, 1882
MaeI	CTAG	3 cut(s)	23, 611, 1275
MaeII	ACGT	3 cut(s)	281, 1755, 1786
MaeIII	GTNAC	3 cut(s)	158, 250, 496
MbiI	CCGCTC	1 cut(s)	2116
MfeI	CAATTG	1 cut(s)	1241
MflI	RGATCY	1 cut(s)	1954
MhlI	GDGCHC	1 cut(s)	1503
MluI	ACGCGT	1 cut(s)	890
MlyI	GAGTC	2 cut(s)	343, 1633
MmeI	TCCRAC	3 cut(s)	819, 1120, 2013
Mph1103I	ATGCAT	3 cut(s)	1287, 1635, 2107
MroXI	GAANNNNTTC	3 cut(s)	45, 630, 1435
MseI	TTAA	8 cut(s)	278, 401, 552, 1032, 1280, 1290, 1781, 1913
MslI	CAYNNNNRTG	3 cut(s)	131, 174, 1890
MspA1I	CMGCKG	1 cut(s)	835
MspCI	CTTAAG	2 cut(s)	551, 1289
MspI	CCGG	3 cut(s)	431, 1204, 1230
MspR9I	CCNGG	7 cut(s)	600, 660, 988, 1231, 1602, 1716, 2135
MunI	CAATTG	1 cut(s)	1241
Mva1269I	GAATGC	1 cut(s)	115
MvaI	CCWGG	6 cut(s)	600, 660, 988, 1602, 1716, 2135
MvnI	CGCG	2 cut(s)	892, 2155
MwoI	GCNNNNNNNGC	2 cut(s)	309, 1176
NciI	CCSGG	1 cut(s)	1231
NcoI	CCATGG	3 cut(s)	1362, 1692, 1852
NlaIV	GGNNCC	5 cut(s)	908, 924, 984, 993, 1447
NmeAIII	GCCGAG	1 cut(s)	182
NmuCI	GTSAC	2 cut(s)	158, 496
NsiI	ATGCAT	3 cut(s)	1287, 1635, 2107
NspI	RCATGY	3 cut(s)	529, 1211, 2109
PaeI	GCATGC	1 cut(s)	1211
PaeR7I	CTCGAG	1 cut(s)	1641
PagI	TCATGA	2 cut(s)	826, 1503
PasI	CCCWGGG	1 cut(s)	987
PciI	ACATGT	1 cut(s)	525
PciSI	GCTCTTC	2 cut(s)	540, 2106
PcsI	WCGNNNNNNNCGW	1 cut(s)	1792
PctI	GAATGC	1 cut(s)	115
PdmI	GAANNNNTTC	3 cut(s)	45, 630, 1435
PfeI	GAWTC	7 cut(s)	95, 748, 1001, 1006, 1084, 1151, 2230
PfoI	TCCNGGA	1 cut(s)	2133
PkrI	GCNGC	9 cut(s)	568, 696, 819, 1418, 1542, 1769, 1816, 2118, 2206
PleI	GAGTC	2 cut(s)	342, 1633
PmaCI	CACGTG	1 cut(s)	1787
PmlI	CACGTG	1 cut(s)	1787
PpsI	GAGTC	2 cut(s)	342, 1633
Ppu21I	YACGTR	1 cut(s)	1787
PscI	ACATGT	1 cut(s)	525
PshBI	ATTAAT	1 cut(s)	1913
Psp124BI	GAGCTC	1 cut(s)	1503
Psp6I	CCWGG	6 cut(s)	598, 658, 986, 1600, 1714, 2133
PspCI	CACGTG	1 cut(s)	1787
PspFI	CCCAGC	1 cut(s)	329
PspGI	CCWGG	6 cut(s)	598, 658, 986, 1600, 1714, 2133
PspN4I	GGNNCC	5 cut(s)	908, 924, 984, 993, 1447
PspPI	GGNCC	2 cut(s)	149, 992
PspXI	VCTCGAGB	1 cut(s)	1641
PstI	CTGCAG	1 cut(s)	669
PstNI	CAGNNNCTG	3 cut(s)	853, 1540, 2140
PsuI	RGATCY	1 cut(s)	1954
RsaI	GTAC	2 cut(s)	1022, 1977
RsaNI	GTAC	2 cut(s)	1021, 1976
RseI	CAYNNNNRTG	3 cut(s)	131, 174, 1890
SacI	GAGCTC	1 cut(s)	1503
SapI	GCTCTTC	2 cut(s)	540, 2106
SaqAI	TTAA	8 cut(s)	278, 401, 552, 1032, 1280, 1290, 1781, 1913
SatI	GCNGC	9 cut(s)	567, 695, 818, 1417, 1541, 1768, 1815, 2117, 2205
Sau96I	GGNCC	2 cut(s)	149, 992
SchI	GAGTC	2 cut(s)	343, 1633
ScrFI	CCNGG	7 cut(s)	600, 660, 988, 1231, 1602, 1716, 2135
SduI	GDGCHC	1 cut(s)	1503
SfaNI	GCATC	6 cut(s)	396, 700, 783, 859, 1294, 1882
SfcI	CTRYAG	1 cut(s)	665
Sfr274I	CTCGAG	1 cut(s)	1641
SinI	GGWCC	2 cut(s)	149, 992
SlaI	CTCGAG	1 cut(s)	1641
SmiMI	CAYNNNNRTG	3 cut(s)	131, 174, 1890
SmlI	CTYRAG	4 cut(s)	551, 1289, 1641, 1950
SmoI	CTYRAG	4 cut(s)	551, 1289, 1641, 1950
SphI	GCATGC	1 cut(s)	1211
SsiI	CCGC	6 cut(s)	560, 835, 1417, 1849, 2116, 2153
SspI	AATATT	1 cut(s)	1468
SspMI	CTAG	3 cut(s)	23, 611, 1275
SstI	GAGCTC	1 cut(s)	1503
StyD4I	CCNGG	7 cut(s)	598, 658, 986, 1229, 1600, 1714, 2133
StyI	CCWWGG	4 cut(s)	902, 1362, 1692, 1852
TaaI	ACNGT	2 cut(s)	1776, 1829
TaiI	ACGT	3 cut(s)	284, 1758, 1789
TaqI	TCGA	6 cut(s)	342, 1004, 1190, 1222, 1461, 1642
TatI	WGTACW	1 cut(s)	1975
TauI	GCSGC	2 cut(s)	1419, 2119
TfiI	GAWTC	7 cut(s)	95, 748, 1001, 1006, 1084, 1151, 2230
Tru1I	TTAA	8 cut(s)	278, 401, 552, 1032, 1280, 1290, 1781, 1913
Tru9I	TTAA	8 cut(s)	278, 401, 552, 1032, 1280, 1290, 1781, 1913
TscAI	CASTG	2 cut(s)	321, 627
TseFI	GTSAC	2 cut(s)	158, 496
TseI	GCWGC	7 cut(s)	566, 694, 817, 1540, 1767, 1814, 2204
Tsp45I	GTSAC	2 cut(s)	158, 496
TspGWI	ACGGA	1 cut(s)	1992
TspRI	CASTG	2 cut(s)	321, 627
Vha464I	CTTAAG	2 cut(s)	551, 1289
VpaK11BI	GGWCC	2 cut(s)	149, 992
VspI	ATTAAT	1 cut(s)	1913
XapI	RAATTY	5 cut(s)	35, 213, 589, 1383, 1575
XceI	RCATGY	3 cut(s)	529, 1211, 2109
XhoI	CTCGAG	1 cut(s)	1641
XmiI	GTMKAC	1 cut(s)	810
XmnI	GAANNNNTTC	3 cut(s)	45, 630, 1435
XspI	CTAG	3 cut(s)	23, 611, 1275
Zsp2I	ATGCAT	3 cut(s)	1287, 1635, 2107

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.