pycom03g00300

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001707
Physical Location & Seq
Forward (+)
258506 .. 260685
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00300.5

Sequence Viewer

Length: 1464 bp
ATGGAAGAGGAGCAATACTATAGGATGCAGCAGCGATATGTGTACTTGCAGCAGCTTCATGATCAGCGGTTGGAAGCTCAGCATCTGGTTCAAGGGAATGGGAATATTGCAAGCAGGCTCACGTGTCCATCCCCAAGGCACCCCCATTTTGAGGTGCCACTTGAACAATCTAATCAAGAACAAATATGGAACAATTATGTGGTTCCCAGGGGTCCACACCAATCAAATCCCTTTCTTTCATACACAGATTGTAATGGCATTCAAGTTCTTGACAAAGTGGGAAAGCAGAGTTTTCCTGAGAAGATTCTAACACGATCACATGGGATGAACACACTTAAAGCTGTGAAGTTCGGTGCTATTGGAACAGATGAATCACTTACGCATGCTAGCCAGAATGGCAAAGCTCTCCCAAACGGTCATTTCCGGCATGCCTTATCTACTCAAAATACCGGATGCTTTCAATTGGATAGTTTGAACACATGGGGTATGTTCCCTAGTTTAATGCATCTTAAGAATACGGATATGAAGGCGCTACCTCAGAAATATACTGATCTGAAGACAGTGCCTCAGAAATATAACTCCATGGATGAAATTACTGGAAGAATTTATCTAATGGCTAAGGACCAGCATGGTTGCCGCTTCTTGCAAAGAAAGTTTTCTGAAGGCACCCAAAAAGATGTCGAAAATATTTTTTCTGAGATCATTGATCACATTGTTGAGCTCATGACAGACCCTTTTGGGAATTACCTCATTCAGAAGCTGCTTGAAGTGTGTGATGAGGATCAGCAAATGCAAATTCTTCATTCAATCACTAAAAAGCCAGGGGAACTTGTTAGGATTTCATGTGATATGCATGGGACTCGGGCTGTTCAAAAGGTTATTGAAACCCTCAAAATTGCAGAGCAGTTTTCCATGGTTGTTTCATCGCTAAAGCCAGGGATAGTGACTTTGATAAAGAACACAAATGGAAATCACGTTGCACAGCGCTGCTTACAGTATTTAACGCCTGAATATCGTGAGTTTCTTTTCGCAGCTGCAACTACTAACTGTGTAGAGCTTGCAACTGACCGCCATGGTTGTTGTGTGCTACAAAAATGCCTTAACCACTCGGATGCTGAACAAAGAAACCGATTAATCTGCGAGATTACTTCAAATGCACTAATCCTTTCTCAAGATCCATTTGGGAACTATGTTGTGCAATTTGTCCTTGAACTTCAGCTTCCGTGGGCAACTGTAGATATTCTTGACCAGTTGGAGGGTAATTATGGGGATTTGTCAGTGCAGAAGTATAGCAGTAATGTGGTTGAGAAAAGCCTGAAATATGCAGATGAAGAGCGTCGCACGCGCATTATTCAGGAGCTGATAGAAAATCCGCGCTTGGATCGAGTCATGCAAGACCCTTATGGAAATTATGTTGTCCAAGCAGCTCTCAGTCAATCAAAGTTGTGGAATCCTTCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

488

Amino Acids

55.77

Weight (kDa)

6.29

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 138, 154, 665
AccII CGCG 2 cut(s) 1345, 1375
AciI CCGC 4 cut(s) 67, 637, 1069, 1373
AclWI GGATC 3 cut(s) 789, 1169, 1389
AcsI RAATTY 2 cut(s) 603, 795
AcuI CTGAAG 3 cut(s) 575, 681, 1199
AcvI CACGTG 1 cut(s) 123
AfaI GTAC 1 cut(s) 44
AfeI AGCGCT 1 cut(s) 986
AfiI CCNNNNNNNGG 2 cut(s) 151, 1255
AflII CTTAAG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 122
AjnI CCWGG 3 cut(s) 206, 820, 934
Alw21I GWGCWC 1 cut(s) 723
AlwI GGATC 3 cut(s) 789, 1169, 1389
AlwNI CAGNNNCTG 3 cut(s) 85, 760, 1360
Ama87I CYCGRG 1 cut(s) 861
Aor51HI AGCGCT 1 cut(s) 986
ApeKI GCWGC 9 cut(s) 28, 31, 49, 52, 760, 987, 1031, 1034, 1424
ApoI RAATTY 2 cut(s) 603, 795
AseI ATTAAT 1 cut(s) 1133
Asp700I GAANNNNTTC 1 cut(s) 655
AspLEI GCGC 4 cut(s) 532, 987, 1347, 1377
AspS9I GGNCC 2 cut(s) 212, 622
AsuNHI GCTAGC 1 cut(s) 386
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 2 cut(s) 212, 622
BanI GGYRCC 3 cut(s) 138, 154, 665
BanII GRGCYC 1 cut(s) 723
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 9 cut(s) 40, 43, 61, 64, 747, 974, 1021, 1043, 1436
BccI CCATC 1 cut(s) 136
BcgI CGANNNNNNTGC 4 cut(s) 842, 876, 1119, 1153
BciT130I CCWGG 3 cut(s) 208, 822, 936
BclI TGATCA 2 cut(s) 61, 706
BfaI CTAG 2 cut(s) 387, 495
BfmI CTRYAG 2 cut(s) 19, 1233
BfoI RGCGCY 2 cut(s) 533, 988
BfrI CTTAAG 1 cut(s) 509
BglI GCCNNNNNGGC 1 cut(s) 396
BlpI GCTNAGC 1 cut(s) 78
Bme1390I CCNGG 3 cut(s) 208, 822, 936
Bme18I GGWCC 2 cut(s) 212, 622
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 2 cut(s) 212, 622
BmiI GGNNCC 5 cut(s) 140, 156, 204, 213, 667
BmrFI CCNGG 3 cut(s) 208, 822, 936
BmsI GCATC 5 cut(s) 15, 91, 443, 514, 1102
BmtI GCTAGC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 618
Bpu1102I GCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 1155
BsaAI YACGTR 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 9 cut(s) 134, 206, 207, 582, 821, 912, 935, 1072, 1223
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 1255
Bse1I ACTGG 2 cut(s) 601, 1249
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 3 cut(s) 208, 822, 936
BseDI CCNNGG 9 cut(s) 134, 206, 207, 582, 821, 912, 935, 1072, 1223
BseGI GGATG 6 cut(s) 30, 128, 330, 458, 592, 1117
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 151, 1255
BseMII CTCAG 6 cut(s) 92, 288, 551, 581, 687, 1444
BseNI ACTGG 2 cut(s) 601, 1249
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 9 cut(s) 40, 43, 61, 64, 747, 974, 1021, 1043, 1436
BsgI GTGCAG 1 cut(s) 1301
Bsh1236I CGCG 2 cut(s) 1345, 1375
BshNI GGYRCC 3 cut(s) 138, 154, 665
BsiHKAI GWGCWC 1 cut(s) 723
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 2 cut(s) 424, 450
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 2 cut(s) 151, 1255
BsmFI GGGAC 1 cut(s) 871
BsmI GAATGC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 723
Bsp143I GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
Bsp1720I GCTNAGC 1 cut(s) 78
Bsp19I CCATGG 3 cut(s) 582, 912, 1072
BspACI CCGC 4 cut(s) 67, 637, 1069, 1373
BspCNI CTCAG 6 cut(s) 91, 289, 550, 580, 688, 1443
BspFNI CGCG 2 cut(s) 1345, 1375
BspHI TCATGA 2 cut(s) 58, 723
BspLI GGNNCC 5 cut(s) 140, 156, 204, 213, 667
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 3 cut(s) 789, 1169, 1389
BspQI GCTCTTC 1 cut(s) 1326
BspT107I GGYRCC 3 cut(s) 138, 154, 665
BspTI CTTAAG 1 cut(s) 509
BsrI ACTGG 2 cut(s) 601, 1249
BssECI CCNNGG 9 cut(s) 134, 206, 207, 582, 821, 912, 935, 1072, 1223
BssMI GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
BssT1I CCWWGG 4 cut(s) 134, 582, 912, 1072
Bst2UI CCWGG 3 cut(s) 208, 822, 936
Bst4CI ACNGT 5 cut(s) 416, 562, 996, 1049, 1234
Bst6I CTCTTC 1 cut(s) 1326
BstAFI CTTAAG 1 cut(s) 509
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 7 cut(s) 112, 116, 384, 388, 429, 1059, 1343
BstDEI CTNAG 7 cut(s) 78, 297, 537, 567, 618, 696, 1430
BstDSI CCRYGG 4 cut(s) 582, 912, 1072, 1223
BstF5I GGATG 6 cut(s) 30, 128, 330, 458, 592, 1117
BstFNI CGCG 2 cut(s) 1345, 1375
BstH2I RGCGCY 2 cut(s) 533, 988
BstHHI GCGC 4 cut(s) 532, 987, 1347, 1377
BstKTI GATC 8 cut(s) 64, 317, 553, 702, 709, 784, 1177, 1384
BstMBI GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
BstMWI GCNNNNNNNGC 2 cut(s) 396, 1342
BstNI CCWGG 3 cut(s) 208, 822, 936
BstNSI RCATGY 2 cut(s) 386, 431
BstSCI CCNGG 3 cut(s) 206, 820, 934
BstSFI CTRYAG 2 cut(s) 19, 1233
BstUI CGCG 2 cut(s) 1345, 1375
BstV1I GCAGC 9 cut(s) 40, 43, 61, 64, 747, 974, 1021, 1043, 1436
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 1 cut(s) 1174
BstYI RGATCY 1 cut(s) 1174
BtgI CCRYGG 4 cut(s) 582, 912, 1072, 1223
BtgZI GCGATG 1 cut(s) 909
BtsCI GGATG 6 cut(s) 30, 128, 330, 458, 592, 1117
BtsIMutI CAGTG 2 cut(s) 567, 1284
Cac8I GCNNGC 7 cut(s) 112, 116, 384, 388, 429, 1059, 1343
CaiI CAGNNNCTG 3 cut(s) 85, 760, 1360
CciI TCATGA 2 cut(s) 58, 723
CfoI GCGC 4 cut(s) 532, 987, 1347, 1377
Cfr13I GGNCC 2 cut(s) 212, 622
CseI GACGC 1 cut(s) 1325
Csp6I GTAC 1 cut(s) 43
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 7 cut(s) 78, 297, 537, 567, 618, 696, 1430
DpnI GATC 8 cut(s) 63, 316, 552, 701, 708, 783, 1176, 1383
DpnII GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
Eam1104I CTCTTC 1 cut(s) 1326
EarI CTCTTC 1 cut(s) 1326
Ecl136II GAGCTC 1 cut(s) 721
Eco130I CCWWGG 4 cut(s) 134, 582, 912, 1072
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 2 cut(s) 212, 622
Eco47III AGCGCT 1 cut(s) 986
Eco53kI GAGCTC 1 cut(s) 721
Eco57I CTGAAG 3 cut(s) 575, 681, 1199
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 861
EcoICRI GAGCTC 1 cut(s) 721
EcoRII CCWGG 3 cut(s) 206, 820, 934
EcoT14I CCWWGG 4 cut(s) 134, 582, 912, 1072
EcoT22I ATGCAT 2 cut(s) 507, 855
EcoT38I GRGCYC 1 cut(s) 723
ErhI CCWWGG 4 cut(s) 134, 582, 912, 1072
FaqI GGGAC 1 cut(s) 871
FbaI TGATCA 2 cut(s) 61, 706
FokI GGATG 6 cut(s) 37, 115, 337, 465, 599, 1124
FriOI GRGCYC 1 cut(s) 723
FspBI CTAG 2 cut(s) 387, 495
GlaI GCGC 4 cut(s) 531, 986, 1346, 1376
HaeII RGCGCY 2 cut(s) 533, 988
HapII CCGG 2 cut(s) 424, 450
HgaI GACGC 1 cut(s) 1325
HhaI GCGC 4 cut(s) 532, 987, 1347, 1377
Hin6I GCGC 4 cut(s) 530, 985, 1345, 1375
HinP1I GCGC 4 cut(s) 530, 985, 1345, 1375
HinfI GANTC 5 cut(s) 304, 371, 859, 1386, 1450
HpaII CCGG 2 cut(s) 424, 450
Hpy166II GTNNAC 2 cut(s) 43, 215
Hpy188I TCNGA 7 cut(s) 540, 555, 570, 661, 697, 756, 1111
Hpy188III TCNNGA 9 cut(s) 59, 176, 269, 296, 724, 1016, 1172, 1244, 1355
Hpy8I GTNNAC 2 cut(s) 43, 215
Hpy99I CGWCG 1 cut(s) 1341
HpyAV CCTTC 3 cut(s) 520, 656, 1464
HpyCH4III ACNGT 5 cut(s) 416, 562, 996, 1049, 1234
HpyCH4IV ACGT 2 cut(s) 122, 975
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 1342
HpyF3I CTNAG 7 cut(s) 78, 297, 537, 567, 618, 696, 1430
HpySE526I ACGT 2 cut(s) 122, 975
HspAI GCGC 4 cut(s) 530, 985, 1345, 1375
Ksp22I TGATCA 2 cut(s) 61, 706
Kzo9I GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
LguI GCTCTTC 1 cut(s) 1326
LmnI GCTCC 2 cut(s) 10, 1357
Lsp1109I GCAGC 9 cut(s) 40, 43, 61, 64, 747, 974, 1021, 1043, 1436
LweI GCATC 5 cut(s) 15, 91, 443, 514, 1102
MaeI CTAG 2 cut(s) 387, 495
MaeII ACGT 2 cut(s) 122, 975
MaeIII GTNAC 1 cut(s) 943
MalI GATC 8 cut(s) 63, 316, 552, 701, 708, 783, 1176, 1383
MboI GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
MboII GAAGA 6 cut(s) 17, 313, 568, 612, 791, 1343
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 1 cut(s) 1174
MhlI GDGCHC 1 cut(s) 723
MlyI GAGTC 2 cut(s) 853, 1395
MmeI TCCRAC 2 cut(s) 51, 1233
MnlI CCTC 7 cut(s) 145, 546, 576, 758, 772, 899, 1249
Mph1103I ATGCAT 2 cut(s) 507, 855
MroXI GAANNNNTTC 1 cut(s) 655
MseI TTAA 6 cut(s) 336, 500, 510, 1001, 1101, 1133
MslI CAYNNNNRTG 1 cut(s) 1110
MspA1I CMGCKG 2 cut(s) 67, 1034
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 2 cut(s) 424, 450
MspR9I CCNGG 3 cut(s) 208, 822, 936
MunI CAATTG 1 cut(s) 461
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 3 cut(s) 208, 822, 936
MvnI CGCG 2 cut(s) 1345, 1375
MwoI GCNNNNNNNGC 2 cut(s) 396, 1342
NcoI CCATGG 3 cut(s) 582, 912, 1072
NdeII GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
NheI GCTAGC 1 cut(s) 386
NlaIV GGNNCC 5 cut(s) 140, 156, 204, 213, 667
NmuCI GTSAC 1 cut(s) 943
NsiI ATGCAT 2 cut(s) 507, 855
NspI RCATGY 2 cut(s) 386, 431
PaeI GCATGC 2 cut(s) 386, 431
PagI TCATGA 2 cut(s) 58, 723
PasI CCCWGGG 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 1326
PctI GAATGC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 655
PfeI GAWTC 3 cut(s) 304, 371, 1450
PleI GAGTC 2 cut(s) 853, 1394
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 2 cut(s) 853, 1394
Ppu21I YACGTR 1 cut(s) 123
PshBI ATTAAT 1 cut(s) 1133
Psp124BI GAGCTC 1 cut(s) 723
Psp6I CCWGG 3 cut(s) 206, 820, 934
PspCI CACGTG 1 cut(s) 123
PspGI CCWGG 3 cut(s) 206, 820, 934
PspN4I GGNNCC 5 cut(s) 140, 156, 204, 213, 667
PspPI GGNCC 2 cut(s) 212, 622
PstNI CAGNNNCTG 3 cut(s) 85, 760, 1360
PsuI RGATCY 1 cut(s) 1174
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 1110
SacI GAGCTC 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 1326
SaqAI TTAA 6 cut(s) 336, 500, 510, 1001, 1101, 1133
Sau3AI GATC 8 cut(s) 61, 314, 550, 699, 706, 781, 1174, 1381
Sau96I GGNCC 2 cut(s) 212, 622
SchI GAGTC 2 cut(s) 853, 1395
ScrFI CCNGG 3 cut(s) 208, 822, 936
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 5 cut(s) 15, 91, 443, 514, 1102
SfcI CTRYAG 2 cut(s) 19, 1233
SinI GGWCC 2 cut(s) 212, 622
SmiMI CAYNNNNRTG 1 cut(s) 1110
SmlI CTYRAG 2 cut(s) 509, 1170
SmoI CTYRAG 2 cut(s) 509, 1170
SphI GCATGC 2 cut(s) 386, 431
SsiI CCGC 4 cut(s) 67, 637, 1069, 1373
SspI AATATT 2 cut(s) 106, 688
SspMI CTAG 2 cut(s) 387, 495
SstI GAGCTC 1 cut(s) 723
StyD4I CCNGG 3 cut(s) 206, 820, 934
StyI CCWWGG 4 cut(s) 134, 582, 912, 1072
TaaI ACNGT 5 cut(s) 416, 562, 996, 1049, 1234
TaiI ACGT 2 cut(s) 125, 978
TaqI TCGA 2 cut(s) 681, 1384
TatI WGTACW 1 cut(s) 42
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 3 cut(s) 304, 371, 1450
Tru1I TTAA 6 cut(s) 336, 500, 510, 1001, 1101, 1133
Tru9I TTAA 6 cut(s) 336, 500, 510, 1001, 1101, 1133
TscAI CASTG 2 cut(s) 567, 1284
TseFI GTSAC 1 cut(s) 943
TseI GCWGC 9 cut(s) 28, 31, 49, 52, 760, 987, 1031, 1034, 1424
Tsp45I GTSAC 1 cut(s) 943
TspGWI ACGGA 2 cut(s) 533, 1212
TspRI CASTG 2 cut(s) 567, 1284
Vha464I CTTAAG 1 cut(s) 509
VpaK11BI GGWCC 2 cut(s) 212, 622
VspI ATTAAT 1 cut(s) 1133
XapI RAATTY 2 cut(s) 603, 795
XceI RCATGY 2 cut(s) 386, 431
XmnI GAANNNNTTC 1 cut(s) 655
XspI CTAG 2 cut(s) 387, 495
Zsp2I ATGCAT 2 cut(s) 507, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.