Rw5G050200

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001707
Physical Location & Seq
Reverse (-)
86784328 .. 86788898
4571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050200.1

Sequence Viewer

Length: 2268 bp
ATGGAAGGAGGGAGAACTGAGCTAGAGTTCGATGAGTTTGAGAAGCTCCTTGGTGAGATACCGAATGCCACTTCACATTCTGAGGAATCTGGCACCAAGGCTGTATCCTTGAATGGAAGTTTGTCTCCCATTTATGTCAAGTCCTCTTACAAAGGAGGGCCTCTGGGTAAAAAACTCAATAACAATAATGGGAGGCTGGATGAGGGAAAGAACTCGGTGAAGAAAATTCAAAAATCGCCTGCAAAACGGGTTCAACCAGCAGAGGTATCAAATCTGCCTGATGATCAATCCTTAACATCTGCATTTGCAGGACTCAGCTTTGATGATGGCCAAATCCCAAACACCTTGAAGCCACAGCTTTCGAGTATGGATACTCCTCTTATGGTTGTTCCACCTTTCCAAGCAGCAACTGGCATATCTTGTGGTTTTTACAAGTTTGATTTCACAAACATAGGCCAAGAAAGTTCAAACATGTCAAAATTCAGTTCAGAAGAGCTTAAGAAACCTCAAGTTCCTATTGGAAATTTGTCATCAGCTGTTCCTGTAGCTCATGCTGTACAAGGTTTTCAGTTCCTCTCAAATGTGCCTGCTCCTGGTTTGCAGTTTCCTCTAACATCTGAACAGCAGCAATTCTTTTTGGATGCACAATCTCGTTTTCCATACCTACATTCACAACAACATCAAGTTAGTTGGAGGAATATCCAAGAGGAACAATATTTTAGGATGCAGCAGCGGTATGTGTACTTGCAGCAACTTCATGATCAGCGGTTGGAAGCTCCAAATATGGTTCAAGACAGAGGCGACATTGCTAGCAGACTAACTTGTCCGAACCCCAGGCACCCCCATTTTGAGGTGCCAATTTCTCACCGACTGGAGCAATCTAATCAAGAACAAATTTGGAACAATTATGTGATTCCTAGGGGTCCAAACCAATCAAGTACTCCTGCACTATCCTACACAGATTTTAAAAGGATTCATGTTCTTGACAAAGTGGGAAAACAGAATGTTCCTGAGAAGATTCTAACACGTTCACATGGAATGAACACAATCAAAGCTGTGAAATTTGGTGCTGTTGGAGCAGCAGATGAATCACTTGGTCATGTTAGTCCAAATGGAAAAGTTGTCTCAAATGGTCATCTCCGGCATAACTTATCTACCCCAAATGCCGGTAACTTTCAATTGGATAATGTGAGCACATGGGGTACGTTCCCTGGTATAATGCATCTTAAGAGTACAGATTTGAAGGCACTGCCTCAGAAGTATAATGATACAAAGCCATTGCCTCAGAAATATAGCTCAACGGATGAAATAACTGGCAGAATTTATCTCATGGCTAAGGATCAGCATGGCTGCCGGTTCTTACAAAGAAAATTTTCTGAAGGAGCACGAAAAGATGTTGAGAATATATTTCTTGAAATCATAGATCACATTGTTGAGCTCATGACAGATCCTTTTGGGAATTACCTCATCCAGAAGCTTCTTGAAGTGTGTGATGATGACCAGCAAATGCAAATTCTTCATTCAATCACCAAAAAATCAGGAGAACTTGTTAATATTTCCTGTGATATGCATGGGACAAGGGCTGTTCAAAAGGTTATTGAAACCCTCAAAACTCCAGAGCAGTTTTCCATGGTTGTTTCCTCACTAAAGCCTGGGATAGTGAACTTGATAAAGAACACAAATGGCAACCACGTTGCGCAGCGCTGCTTGCAGTATTTAACACCTGAATATCGTGAGTTTCTTTTTGAAGCTGCAACTAGTAATTGTGTTGAGCTTGCAACCGACCGTCATGGCTGTTGTGTGCTTCAAAAATGTCTTAGTCATTCTGATGGTGAGCAAAGAGACCGATTGATCTGCAAGATCACTTCCAATGCACTAATCCTTTCCCAAGATCCATTTGGGAACTATGTCGTGCAATTCGTCTTTGAGCTTCAGCTTCCGTGGGCGACAGTAGACATTCTTGAGCAGTTGGAGGGTAATTATGGGGATTTGTCTGTGCAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGCCGCGCTCGCATTATTCAGGAACTGATAGGGAATATGCGATTGGACCAAATCATGCAAGACCCTTATGGAAATTATGTTATCCAAGCAGCACTCAGTCAATCAAAGGGAACACTTCATTCTAAACTGGTAGAAGCAATAAAACCTCATATGCCAGTTCTCCGGACCAGTCCCTATGGGAAGAAAATCCTCTCTACCAATATTTTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

755

Amino Acids

84.68

Weight (kDa)

7.91

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 440 - 474 9.3e-09 Pumilio-family RNA binding repeat
PUF PF00806 476 - 509 8.1e-12 Pumilio-family RNA binding repeat
PUF PF00806 588 - 622 1.6e-15 Pumilio-family RNA binding repeat
PUF PF00806 661 - 694 1.7e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2033
AasI GACNNNNNNGTC 1 cut(s) 822
Acc16I TGCGCA 1 cut(s) 1698
Acc36I ACCTGC 1 cut(s) 2033
AccB1I GGYRCC 3 cut(s) 92, 837, 853
AccI GTMKAC 1 cut(s) 1953
AccII CGCG 1 cut(s) 2058
AccIII TCCGGA 1 cut(s) 2214
AciI CCGC 3 cut(s) 733, 766, 2056
AclWI GGATC 3 cut(s) 1347, 1442, 1886
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 8 cut(s) 225, 479, 523, 894, 1061, 1320, 1370, 1512
AcuI CTGAAG 2 cut(s) 1398, 1916
AfaI GTAC 5 cut(s) 558, 743, 940, 1204, 1234
AfeI AGCGCT 1 cut(s) 1703
AfiI CCNNNNNNNGG 3 cut(s) 593, 850, 1166
AflII CTTAAG 2 cut(s) 497, 1226
AflIII ACRYGT 2 cut(s) 471, 1025
AhlI ACTAGT 1 cut(s) 1757
AjnI CCWGG 4 cut(s) 592, 833, 1210, 1651
AjuI GAANNNNNNNTTGG 2 cut(s) 2078, 2110
Alw21I GWGCWC 3 cut(s) 1196, 1387, 1440
Alw26I GTCTC 3 cut(s) 129, 1129, 1836
AlwI GGATC 3 cut(s) 1347, 1442, 1886
AlwNI CAGNNNCTG 2 cut(s) 410, 2077
Aor13HI TCCGGA 1 cut(s) 2214
Aor51HI AGCGCT 1 cut(s) 1703
AoxI GGCC 3 cut(s) 158, 328, 454
ApoI RAATTY 8 cut(s) 225, 479, 523, 894, 1061, 1320, 1370, 1512
Asp700I GAANNNNTTC 1 cut(s) 1372
AspA2I CCTAGG 1 cut(s) 917
AspLEI GCGC 4 cut(s) 1699, 1704, 2055, 2060
AspS9I GGNCC 4 cut(s) 158, 923, 2098, 2217
AsuHPI GGTGA 6 cut(s) 65, 229, 857, 1519, 1844, 2057
AsuNHI GCTAGC 1 cut(s) 809
AvaII GGWCC 3 cut(s) 923, 2098, 2217
AvrII CCTAGG 1 cut(s) 917
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 3 cut(s) 92, 837, 853
BanII GRGCYC 1 cut(s) 1440
Bbv12I GWGCWC 3 cut(s) 1196, 1387, 1440
BccI CCATC 2 cut(s) 320, 1823
BcgI CGANNNNNNTGC 2 cut(s) 1836, 1870
BciT130I CCWGG 4 cut(s) 594, 835, 1212, 1653
BciVI GTATCC 2 cut(s) 115, 364
BclI TGATCA 2 cut(s) 283, 760
BcoDI GTCTC 3 cut(s) 129, 1129, 1836
BcuI ACTAGT 1 cut(s) 1757
BfaI CTAG 4 cut(s) 23, 810, 918, 1758
BfmI CTRYAG 1 cut(s) 543
BfoI RGCGCY 2 cut(s) 1705, 2056
BfrI CTTAAG 2 cut(s) 497, 1226
BfuAI ACCTGC 1 cut(s) 2033
BfuI GTATCC 2 cut(s) 115, 364
BlnI CCTAGG 1 cut(s) 917
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 4 cut(s) 594, 835, 1212, 1653
Bme18I GGWCC 3 cut(s) 923, 2098, 2217
BmgT120I GGNCC 4 cut(s) 158, 923, 2098, 2217
BmiI GGNNCC 4 cut(s) 94, 839, 855, 924
BmrFI CCNGG 4 cut(s) 594, 835, 1212, 1653
BmsI GCATC 3 cut(s) 631, 714, 1231
BmtI GCTAGC 1 cut(s) 813
BpmI CTGGAG 2 cut(s) 893, 1599
Bpu10I CCTNAGC 1 cut(s) 1335
BpuEI CTTGAG 2 cut(s) 492, 1982
BsaI GGTCTC 1 cut(s) 1836
BsaJI CCNNGG 8 cut(s) 49, 96, 833, 917, 1210, 1629, 1652, 1940
BsaWI WCCGGW 1 cut(s) 2214
BsaXI ACNNNNNCTCC 2 cut(s) 1533, 1563
Bsc4I CCNNNNNNNGG 3 cut(s) 593, 850, 1166
Bse118I RCCGGY 2 cut(s) 1166, 1353
Bse1I ACTGG 6 cut(s) 415, 876, 1318, 2184, 2207, 2220
Bse3DI GCAATG 2 cut(s) 804, 1277
BseAI TCCGGA 1 cut(s) 2214
BseBI CCWGG 4 cut(s) 594, 835, 1212, 1653
BseDI CCNNGG 8 cut(s) 49, 96, 833, 917, 1210, 1629, 1652, 1940
BseGI GGATG 5 cut(s) 205, 646, 729, 1309, 1467
BseLI CCNNNNNNNGG 3 cut(s) 593, 850, 1166
BseMI GCAATG 2 cut(s) 804, 1277
BseMII CTCAG 7 cut(s) 9, 72, 328, 1002, 1268, 1298, 2161
BseNI ACTGG 6 cut(s) 415, 876, 1318, 2184, 2207, 2220
BseRI GAGGAG 1 cut(s) 366
BsgI GTGCAG 2 cut(s) 930, 2018
Bsh1236I CGCG 1 cut(s) 2058
Bsh1285I CGRYCG 1 cut(s) 1786
BshFI GGCC 3 cut(s) 160, 330, 456
BshNI GGYRCC 3 cut(s) 92, 837, 853
BsiEI CGRYCG 1 cut(s) 1786
BsiHKAI GWGCWC 3 cut(s) 1196, 1387, 1440
BsiSI CCGG 4 cut(s) 1141, 1167, 1354, 2215
BslFI GGGAC 2 cut(s) 1588, 2208
BslI CCNNNNNNNGG 3 cut(s) 593, 850, 1166
BsmAI GTCTC 3 cut(s) 129, 1129, 1836
BsmFI GGGAC 2 cut(s) 1588, 2208
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 3 cut(s) 160, 330, 456
Bso31I GGTCTC 1 cut(s) 1836
Bsp1286I GDGCHC 3 cut(s) 1196, 1387, 1440
Bsp13I TCCGGA 1 cut(s) 2214
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
Bsp19I CCATGG 1 cut(s) 1629
BspACI CCGC 3 cut(s) 733, 766, 2056
BspANI GGCC 3 cut(s) 160, 330, 456
BspCNI CTCAG 7 cut(s) 10, 73, 327, 1003, 1267, 1297, 2160
BspEI TCCGGA 1 cut(s) 2214
BspFNI CGCG 1 cut(s) 2058
BspHI TCATGA 2 cut(s) 757, 1440
BspLI GGNNCC 4 cut(s) 94, 839, 855, 924
BspMI ACCTGC 1 cut(s) 2033
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 3 cut(s) 1347, 1442, 1886
BspQI GCTCTTC 2 cut(s) 486, 2043
BspT107I GGYRCC 3 cut(s) 92, 837, 853
BspTI CTTAAG 2 cut(s) 497, 1226
BspTNI GGTCTC 1 cut(s) 1836
BsrDI GCAATG 2 cut(s) 804, 1277
BsrFI RCCGGY 2 cut(s) 1166, 1353
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 6 cut(s) 415, 876, 1318, 2184, 2207, 2220
BssAI RCCGGY 2 cut(s) 1166, 1353
BssECI CCNNGG 8 cut(s) 49, 96, 833, 917, 1210, 1629, 1652, 1940
BssMI GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
BssT1I CCWWGG 4 cut(s) 49, 96, 917, 1629
Bst2UI CCWGG 4 cut(s) 594, 835, 1212, 1653
Bst4CI ACNGT 2 cut(s) 1787, 1951
Bst6I CTCTTC 2 cut(s) 486, 2043
BstAFI CTTAAG 2 cut(s) 497, 1226
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 6 cut(s) 240, 588, 811, 1709, 1776, 2062
BstDEI CTNAG 9 cut(s) 18, 81, 314, 1011, 1254, 1284, 1335, 1817, 2147
BstDSI CCRYGG 2 cut(s) 1629, 1940
BstF5I GGATG 5 cut(s) 205, 646, 729, 1309, 1467
BstFNI CGCG 1 cut(s) 2058
BstH2I RGCGCY 2 cut(s) 1705, 2056
BstHHI GCGC 4 cut(s) 1699, 1704, 2055, 2060
BstKTI GATC 8 cut(s) 286, 763, 1342, 1426, 1450, 1854, 1863, 1894
BstMAI GTCTC 3 cut(s) 129, 1129, 1836
BstMBI GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
BstMCI CGRYCG 1 cut(s) 1786
BstMWI GCNNNNNNNGC 3 cut(s) 1076, 1708, 2061
BstNI CCWGG 4 cut(s) 594, 835, 1212, 1653
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 4 cut(s) 592, 833, 1210, 1651
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 2058
BstX2I RGATCY 2 cut(s) 1447, 1891
BstYI RGATCY 2 cut(s) 1447, 1891
BsuI GTATCC 2 cut(s) 115, 364
BsuRI GGCC 3 cut(s) 160, 330, 456
BtgI CCRYGG 2 cut(s) 1629, 1940
BtsCI GGATG 5 cut(s) 205, 646, 729, 1309, 1467
BtsI GCAGTG 1 cut(s) 1247
BtsIMutI CAGTG 1 cut(s) 1247
BveI ACCTGC 1 cut(s) 2033
Cac8I GCNNGC 6 cut(s) 240, 588, 811, 1709, 1776, 2062
CaiI CAGNNNCTG 2 cut(s) 410, 2077
CciI TCATGA 2 cut(s) 757, 1440
CfoI GCGC 4 cut(s) 1699, 1704, 2055, 2060
Cfr10I RCCGGY 2 cut(s) 1166, 1353
Cfr13I GGNCC 4 cut(s) 158, 923, 2098, 2217
Csp6I GTAC 5 cut(s) 557, 742, 939, 1203, 1233
CviQI GTAC 5 cut(s) 557, 742, 939, 1203, 1233
DdeI CTNAG 9 cut(s) 18, 81, 314, 1011, 1254, 1284, 1335, 1817, 2147
DpnI GATC 8 cut(s) 285, 762, 1341, 1425, 1449, 1853, 1862, 1893
DpnII GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
DraI TTTAAA 1 cut(s) 967
DrdI GACNNNNNNGTC 1 cut(s) 822
DseDI GACNNNNNNGTC 1 cut(s) 822
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 486, 2043
EarI CTCTTC 2 cut(s) 486, 2043
Ecl136II GAGCTC 1 cut(s) 1438
Eco130I CCWWGG 4 cut(s) 49, 96, 917, 1629
Eco24I GRGCYC 1 cut(s) 1440
Eco31I GGTCTC 1 cut(s) 1836
Eco47I GGWCC 3 cut(s) 923, 2098, 2217
Eco47III AGCGCT 1 cut(s) 1703
Eco53kI GAGCTC 1 cut(s) 1438
Eco57I CTGAAG 2 cut(s) 1398, 1916
EcoICRI GAGCTC 1 cut(s) 1438
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 4 cut(s) 592, 833, 1210, 1651
EcoT14I CCWWGG 4 cut(s) 49, 96, 917, 1629
EcoT22I ATGCAT 2 cut(s) 1224, 1572
EcoT38I GRGCYC 1 cut(s) 1440
ErhI CCWWGG 4 cut(s) 49, 96, 917, 1629
FaqI GGGAC 2 cut(s) 1588, 2208
FauNDI CATATG 1 cut(s) 2202
FbaI TGATCA 2 cut(s) 283, 760
FblI GTMKAC 1 cut(s) 1953
FokI GGATG 5 cut(s) 212, 653, 736, 1316, 1454
FriOI GRGCYC 1 cut(s) 1440
FspBI CTAG 4 cut(s) 23, 810, 918, 1758
FspI TGCGCA 1 cut(s) 1698
GlaI GCGC 4 cut(s) 1698, 1703, 2054, 2059
GsuI CTGGAG 2 cut(s) 893, 1599
HaeII RGCGCY 2 cut(s) 1705, 2056
HaeIII GGCC 3 cut(s) 160, 330, 456
HapII CCGG 4 cut(s) 1141, 1167, 1354, 2215
HhaI GCGC 4 cut(s) 1699, 1704, 2055, 2060
Hin6I GCGC 4 cut(s) 1697, 1702, 2053, 2058
HinP1I GCGC 4 cut(s) 1697, 1702, 2053, 2058
HindIII AAGCTT 1 cut(s) 1475
HinfI GANTC 6 cut(s) 86, 312, 913, 973, 1018, 1088
HpaII CCGG 4 cut(s) 1141, 1167, 1354, 2215
HphI GGTGA 6 cut(s) 65, 229, 857, 1519, 1844, 2057
Hpy166II GTNNAC 4 cut(s) 742, 1031, 1663, 1954
Hpy188I TCNGA 8 cut(s) 82, 490, 619, 828, 1257, 1287, 1378, 1828
Hpy8I GTNNAC 4 cut(s) 742, 1031, 1663, 1954
HpyAV CCTTC 2 cut(s) 1237, 1373
HpyCH4III ACNGT 2 cut(s) 1787, 1951
HpyCH4IV ACGT 3 cut(s) 1027, 1205, 1692
HpyF10VI GCNNNNNNNGC 3 cut(s) 1076, 1708, 2061
HpyF3I CTNAG 9 cut(s) 18, 81, 314, 1011, 1254, 1284, 1335, 1817, 2147
HpySE526I ACGT 3 cut(s) 1027, 1205, 1692
HspAI GCGC 4 cut(s) 1697, 1702, 2053, 2058
Kpn2I TCCGGA 1 cut(s) 2214
Ksp22I TGATCA 2 cut(s) 283, 760
Kzo9I GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
LguI GCTCTTC 2 cut(s) 486, 2043
LmnI GCTCC 6 cut(s) 51, 595, 781, 874, 1076, 1382
LweI GCATC 3 cut(s) 631, 714, 1231
MaeI CTAG 4 cut(s) 23, 810, 918, 1758
MaeII ACGT 3 cut(s) 1027, 1205, 1692
MaeIII GTNAC 1 cut(s) 1169
MalI GATC 8 cut(s) 285, 762, 1341, 1425, 1449, 1853, 1862, 1893
MboI GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
MboII GAAGA 6 cut(s) 232, 503, 1027, 1508, 2060, 2245
MfeI CAATTG 1 cut(s) 1178
MflI RGATCY 2 cut(s) 1447, 1891
MhlI GDGCHC 3 cut(s) 1196, 1387, 1440
MlsI TGGCCA 1 cut(s) 330
MluNI TGGCCA 1 cut(s) 330
MlyI GAGTC 1 cut(s) 306
MmeI TCCRAC 4 cut(s) 671, 750, 1054, 1950
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 2 cut(s) 1224, 1572
MroI TCCGGA 1 cut(s) 2214
MroXI GAANNNNTTC 1 cut(s) 1372
MscI TGGCCA 1 cut(s) 330
MseI TTAA 6 cut(s) 293, 498, 966, 1227, 1551, 1718
MslI CAYNNNNRTG 1 cut(s) 1827
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 3 cut(s) 536, 733, 766
MspCI CTTAAG 2 cut(s) 497, 1226
MspI CCGG 4 cut(s) 1141, 1167, 1354, 2215
MspR9I CCNGG 4 cut(s) 594, 835, 1212, 1653
MteI GCGCNGCGC 2 cut(s) 1700, 2056
MunI CAATTG 1 cut(s) 1178
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 4 cut(s) 594, 835, 1212, 1653
MvnI CGCG 1 cut(s) 2058
MwoI GCNNNNNNNGC 3 cut(s) 1076, 1708, 2061
NcoI CCATGG 1 cut(s) 1629
NdeI CATATG 1 cut(s) 2202
NdeII GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
NheI GCTAGC 1 cut(s) 809
NlaIV GGNNCC 4 cut(s) 94, 839, 855, 924
NsbI TGCGCA 1 cut(s) 1698
NsiI ATGCAT 2 cut(s) 1224, 1572
NspI RCATGY 1 cut(s) 475
PagI TCATGA 2 cut(s) 757, 1440
PaqCI CACCTGC 1 cut(s) 2033
PciI ACATGT 1 cut(s) 471
PciSI GCTCTTC 2 cut(s) 486, 2043
PcsI WCGNNNNNNNCGW 1 cut(s) 1917
PctI GAATGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 1372
PfeI GAWTC 5 cut(s) 86, 913, 973, 1018, 1088
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
PscI ACATGT 1 cut(s) 471
Psp124BI GAGCTC 1 cut(s) 1440
Psp6I CCWGG 4 cut(s) 592, 833, 1210, 1651
PspGI CCWGG 4 cut(s) 592, 833, 1210, 1651
PspN4I GGNNCC 4 cut(s) 94, 839, 855, 924
PspPI GGNCC 4 cut(s) 158, 923, 2098, 2217
PstNI CAGNNNCTG 2 cut(s) 410, 2077
PsuI RGATCY 2 cut(s) 1447, 1891
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 5 cut(s) 558, 743, 940, 1204, 1234
RsaNI GTAC 5 cut(s) 557, 742, 939, 1203, 1233
RseI CAYNNNNRTG 1 cut(s) 1827
SacI GAGCTC 1 cut(s) 1440
SapI GCTCTTC 2 cut(s) 486, 2043
SaqAI TTAA 6 cut(s) 293, 498, 966, 1227, 1551, 1718
Sau3AI GATC 8 cut(s) 283, 760, 1339, 1423, 1447, 1851, 1860, 1891
Sau96I GGNCC 4 cut(s) 158, 923, 2098, 2217
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 1 cut(s) 306
ScrFI CCNGG 4 cut(s) 594, 835, 1212, 1653
SduI GDGCHC 3 cut(s) 1196, 1387, 1440
SfaNI GCATC 3 cut(s) 631, 714, 1231
SfcI CTRYAG 1 cut(s) 543
SinI GGWCC 3 cut(s) 923, 2098, 2217
SmiMI CAYNNNNRTG 1 cut(s) 1827
SmlI CTYRAG 4 cut(s) 497, 507, 1226, 1961
SmoI CTYRAG 4 cut(s) 497, 507, 1226, 1961
SpeI ACTAGT 1 cut(s) 1757
SsiI CCGC 3 cut(s) 733, 766, 2056
SspI AATATT 3 cut(s) 716, 1555, 2254
SspMI CTAG 4 cut(s) 23, 810, 918, 1758
SstI GAGCTC 1 cut(s) 1440
StyD4I CCNGG 4 cut(s) 592, 833, 1210, 1651
StyI CCWWGG 4 cut(s) 49, 96, 917, 1629
TaaI ACNGT 2 cut(s) 1787, 1951
TaiI ACGT 3 cut(s) 1030, 1208, 1695
TaqI TCGA 2 cut(s) 30, 362
TaqII GACCGA 1 cut(s) 1860
TatI WGTACW 4 cut(s) 556, 741, 938, 1232
TauI GCSGC 1 cut(s) 2058
TfiI GAWTC 5 cut(s) 86, 913, 973, 1018, 1088
Tru1I TTAA 6 cut(s) 293, 498, 966, 1227, 1551, 1718
Tru9I TTAA 6 cut(s) 293, 498, 966, 1227, 1551, 1718
TscAI CASTG 1 cut(s) 1254
TspDTI ATGAA 7 cut(s) 746, 965, 1055, 1101, 1320, 1508, 2159
TspGWI ACGGA 2 cut(s) 1316, 1929
TspRI CASTG 1 cut(s) 1254
Vha464I CTTAAG 2 cut(s) 497, 1226
VpaK11BI GGWCC 3 cut(s) 923, 2098, 2217
XapI RAATTY 8 cut(s) 225, 479, 523, 894, 1061, 1320, 1370, 1512
XceI RCATGY 1 cut(s) 475
XcmI CCANNNNNNNNNTGG 2 cut(s) 407, 1895
XmaJI CCTAGG 1 cut(s) 917
XmiI GTMKAC 1 cut(s) 1953
XmnI GAANNNNTTC 1 cut(s) 1372
XspI CTAG 4 cut(s) 23, 810, 918, 1758
ZrmI AGTACT 1 cut(s) 940
Zsp2I ATGCAT 2 cut(s) 1224, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.