Rroxscaffold_3G00221790

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001707
Physical Location & Seq
Forward (+)
4700121 .. 4700754
634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221790.1

Sequence Viewer

Length: 396 bp
ATGAGCCCGTGTTACACCGTTGATTATCGGAACTATGTCGTGCAATTCGTCTTTGAGCTTCGGCTTCCGTGGGCGACGAGAGACATTCTTGAGCGGTTGGAAGGTAATTATGGGGATTTGTCCGTGCAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGCCGTGCTCGCATTATTCGAGAACCGATAGGGAATATGCGATTGGACCAAATCATGCAAGACCCTTATGGCAATTATGTTATCCAAGCAGCACTCGGTCAATCAAAGGGAACACTTCATTCTAAACTGGTAGAAGCAATAAAACCTAATATGCCAGTTCTCCGGACCAGTCCCTATGGGAAGAAAATCCTCTCTACCAATATTTTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

131

Amino Acids

15.08

Weight (kDa)

9.57

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 37 - 68 3.6e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 94
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 1 cut(s) 94
AgsI TTSAA 2 cut(s) 152, 388
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 75
Aor13HI TCCGGA 1 cut(s) 342
ApeKI GCWGC 1 cut(s) 269
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 226, 345
AsuHPI GGTGA 1 cut(s) 185
AvaII GGWCC 2 cut(s) 226, 345
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 75
BfoI RGCGCY 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme18I GGWCC 2 cut(s) 226, 345
BmgT120I GGNCC 2 cut(s) 226, 345
BpuEI CTTGAG 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 342
Bse1I ACTGG 3 cut(s) 312, 335, 348
BseAI TCCGGA 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 68
BseNI ACTGG 3 cut(s) 312, 335, 348
BseXI GCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 1 cut(s) 343
BslFI GGGAC 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 8, 190
Bsp13I TCCGGA 1 cut(s) 342
BspACI CCGC 1 cut(s) 94
BspEI TCCGGA 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 161
BspQI GCTCTTC 1 cut(s) 171
BsrBI CCGCTC 1 cut(s) 94
BsrI ACTGG 3 cut(s) 312, 335, 348
BssECI CCNNGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 190
BstDSI CCRYGG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 68
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 190
CfoI GCGC 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 226, 345
CviAII CATG 1 cut(s) 235
CviJI RGCY 4 cut(s) 6, 58, 64, 159
CviKI_1 RGCY 4 cut(s) 6, 58, 64, 159
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 226, 345
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 1 cut(s) 238
FaiI YATR 9 cut(s) 36, 111, 135, 218, 236, 249, 258, 332, 357
FaqI GGGAC 1 cut(s) 336
FatI CATG 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 1 cut(s) 270
GlaI GCGC 1 cut(s) 182
GluI GCNGC 1 cut(s) 270
HaeII RGCGCY 1 cut(s) 184
HapII CCGG 1 cut(s) 343
HhaI GCGC 1 cut(s) 183
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HpaII CCGG 1 cut(s) 343
HphI GGTGA 1 cut(s) 185
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 3 cut(s) 89, 200, 343
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 3 cut(s) 43, 127, 238
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 1 cut(s) 181
Kpn2I TCCGGA 1 cut(s) 342
LguI GCTCTTC 1 cut(s) 171
LpnPI CCDG 6 cut(s) 156, 173, 293, 348, 356, 361
Lsp1109I GCAGC 1 cut(s) 281
MaeIII GTNAC 1 cut(s) 11
MbiI CCGCTC 1 cut(s) 94
MboII GAAGA 2 cut(s) 188, 373
MhlI GDGCHC 2 cut(s) 8, 190
MluCI AATT 3 cut(s) 44, 106, 253
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 1 cut(s) 380
MroI TCCGGA 1 cut(s) 342
MspI CCGG 1 cut(s) 343
MwoI GCNNNNNNNGC 1 cut(s) 189
NlaIII CATG 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 161
PciSI GCTCTTC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 196
PkrI GCNGC 1 cut(s) 271
PspPI GGNCC 2 cut(s) 226, 345
SapI GCTCTTC 1 cut(s) 171
SatI GCNGC 1 cut(s) 270
Sau96I GGNCC 2 cut(s) 226, 345
SduI GDGCHC 2 cut(s) 8, 190
SetI ASST 4 cut(s) 60, 106, 175, 328
SinI GGWCC 2 cut(s) 226, 345
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 3 cut(s) 44, 106, 253
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 382
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 199
TaqII GACCGA 1 cut(s) 266
TasI AATT 3 cut(s) 44, 106, 253
TseI GCWGC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 2 cut(s) 57, 112
VpaK11BI GGWCC 2 cut(s) 226, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.