Rh3AG144600

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001707
Physical Location & Seq
Forward (+)
12800868 .. 12804012
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG144600.1

Sequence Viewer

Length: 507 bp
ATGGTGAGGGACGACGAGGTTGTCGATGTTGTGGAGAAGGAGGACGTGGAGCGGATCAAGGGGTACCCGAAGTTGGGGGTAGGGTCGGTTGGCCCGACGGGTCGTGGAAGTCATTCTGATGGTGAGCAAAGAGACCGATTGATCTGCAAGATCACTTCCAATGCACTAATCCTTTCCCAAGATCCATTTGGGAACTATGTCGTGCAATTCGTCTTTGAGCTTCAGCTTCCGTGGGCGACAGTAGACATTCTTGAGCAGTTGGAGGGTAATTATGGGGATTTGTCTGTGGAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGCCGTGCTCGCATTATTCAGGAACTGATAGGGAATATGCGATTGGACCAAATCATGCAAGACCCTTATGGCAATTATGTTATCCAAGCAGCACTCAGTCAATCAAAGCGGCCATTTTATGAGCGGTGGAAGATGCTTGAGAAAGAGGATTTCGTAAGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF zf-CCCH

Protein Analysis

168

Amino Acids

19.27

Weight (kDa)

4.96

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 91 - 124 1.9e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 323
AasI GACNNNNNNGTC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 323
Acc65I GGTACC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 63
AccBSI CCGCTC 2 cut(s) 52, 466
AccI GTMKAC 1 cut(s) 243
AciI CCGC 3 cut(s) 52, 451, 466
AclWI GGATC 2 cut(s) 62, 176
AcoI YGGCCR 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 206
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 2 cut(s) 73, 74
AgsI TTSAA 1 cut(s) 314
AjiI CACGTC 1 cut(s) 46
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 2 cut(s) 220, 226
AluI AGCT 2 cut(s) 220, 226
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 2 cut(s) 62, 176
AlwNI CAGNNNCTG 1 cut(s) 367
AoxI GGCC 2 cut(s) 91, 452
ApeKI GCWGC 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 112
Asp718I GGTACC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 2 cut(s) 92, 388
AsuHPI GGTGA 3 cut(s) 16, 134, 347
AvaII GGWCC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 1 cut(s) 443
BccI CCATC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 330
BcgI CGANNNNNNTGC 2 cut(s) 126, 160
BcoDI GTCTC 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 323
BisI GCNGC 2 cut(s) 432, 452
BlsI GCNGC 2 cut(s) 433, 453
Bme18I GGWCC 1 cut(s) 388
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 92, 388
BmiI GGNNCC 1 cut(s) 65
BmsI GCATC 1 cut(s) 465
BpuEI CTTGAG 2 cut(s) 272, 500
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 74
BseDI CCNNGG 1 cut(s) 230
BseLI CCNNNNNNNGG 2 cut(s) 73, 74
BseMII CTCAG 1 cut(s) 451
BseXI GCAGC 1 cut(s) 443
BshFI GGCC 2 cut(s) 93, 454
BshNI GGYRCC 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 352
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 73, 74
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 2 cut(s) 93, 454
Bso31I GGTCTC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 4 cut(s) 54, 141, 150, 181
BspACI CCGC 3 cut(s) 52, 451, 466
BspANI GGCC 2 cut(s) 93, 454
BspCNI CTCAG 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 65
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 2 cut(s) 62, 176
BspQI GCTCTTC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 63
BspTNI GGTCTC 1 cut(s) 126
BsrBI CCGCTC 2 cut(s) 52, 466
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 4 cut(s) 54, 141, 150, 181
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 1 cut(s) 437
BstDSI CCRYGG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 4 cut(s) 57, 144, 153, 184
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 4 cut(s) 54, 141, 150, 181
BstMWI GCNNNNNNNGC 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 2 cut(s) 93, 454
BtgI CCRYGG 1 cut(s) 230
BtrI CACGTC 1 cut(s) 46
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 1 cut(s) 352
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 2 cut(s) 92, 388
Csp6I GTAC 1 cut(s) 64
CviAII CATG 1 cut(s) 397
CviJI RGCY 5 cut(s) 93, 220, 226, 321, 454
CviKI_1 RGCY 5 cut(s) 93, 220, 226, 321, 454
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 1 cut(s) 437
DpnI GATC 4 cut(s) 56, 143, 152, 183
DpnII GATC 4 cut(s) 54, 141, 150, 181
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 206
FaeI CATG 1 cut(s) 400
FaiI YATR 8 cut(s) 198, 273, 297, 380, 398, 411, 420, 462
FaqI GGGAC 1 cut(s) 23
FatI CATG 1 cut(s) 396
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 432, 452
Fsp4HI GCNGC 2 cut(s) 432, 452
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 432, 452
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 2 cut(s) 93, 454
HhaI GCGC 1 cut(s) 345
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HphI GGTGA 3 cut(s) 16, 134, 347
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 2 cut(s) 251, 362
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 2 cut(s) 17, 100
HpyAV CCTTC 1 cut(s) 31
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 147, 164, 205, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 351
HpyF3I CTNAG 1 cut(s) 437
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 1 cut(s) 343
KpnI GGTACC 1 cut(s) 67
Kzo9I GATC 4 cut(s) 54, 141, 150, 181
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 3 cut(s) 318, 335, 347
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 1 cut(s) 465
MaeII ACGT 1 cut(s) 45
MalI GATC 4 cut(s) 56, 143, 152, 183
MbiI CCGCTC 2 cut(s) 52, 466
MboI GATC 4 cut(s) 54, 141, 150, 181
MboII GAAGA 2 cut(s) 350, 484
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 3 cut(s) 206, 268, 415
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 4 cut(s) 10, 34, 256, 481
MroXI GAANNNNTTC 1 cut(s) 112
MslI CAYNNNNRTG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 351
NdeII GATC 4 cut(s) 54, 141, 150, 181
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 65
PaqCI CACCTGC 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 333
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 92, 207
PdmI GAANNNNTTC 1 cut(s) 112
PkrI GCNGC 2 cut(s) 433, 453
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 2 cut(s) 92, 388
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 1 cut(s) 181
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 333
SatI GCNGC 2 cut(s) 432, 452
Sau3AI GATC 4 cut(s) 54, 141, 150, 181
Sau96I GGNCC 2 cut(s) 92, 388
SduI GDGCHC 1 cut(s) 352
SetI ASST 5 cut(s) 21, 48, 222, 228, 337
SfaNI GCATC 1 cut(s) 465
SinI GGWCC 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 2 cut(s) 251, 479
SmoI CTYRAG 2 cut(s) 251, 479
Sse9I AATT 3 cut(s) 206, 268, 415
SsiI CCGC 3 cut(s) 52, 451, 466
TaaI ACNGT 1 cut(s) 241
TaiI ACGT 1 cut(s) 48
TaqI TCGA 1 cut(s) 24
TaqII GACCGA 1 cut(s) 150
TasI AATT 3 cut(s) 206, 268, 415
TauI GCSGC 1 cut(s) 454
TseI GCWGC 1 cut(s) 431
TspGWI ACGGA 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 388
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.