Rh1CG090400

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001707
Physical Location & Seq
Reverse (-)
19140014 .. 19141670
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG090400.1

Sequence Viewer

Length: 780 bp
ATGAATGATGACCAGCAAATGCAAATTCTTCATTCAATCACCAAAAAATCAGGAGAACTTGTTAATATTTCCTGTGATATGCATGGGACAAGGGCTGTTCAAAAGGTTATTGAAACCCTCAAAACTCCAGAGCAGTTTTCCATGGTTGTTTCCTCACTAAAGCCTGGGATAGTGAACTTGATAAAGAACACAAATGGCAACCACGTTGCGCAGCGCTGCTTGCAGTATTTAACACCTGAATATCGTGAGTTTCTTTTTGAAGCTGCAACTAGTAATTGTGTTGAGCTTGCAACCGACCGTCATGGCTGTTGTGTGCTTCAAAAATGTCTTAGTCATTCTGATGGTGAGCAAAGAGACCGATTGATCTGCAAGATCACTTCCAATGCACTAATCCTTTCCCAAGATCCATTTGGGAACTATGTCGTGCAATTCGTCTTTGAGCTTCAGCTTCCGTGGGCGACAGTAGACATTCTTGAGCAGTTGGAGGGTAATTATGGGGATTTGTCTGTGCAGAAGTATAGCAGTAATGTGGTTGAAAAAAGCCTGAAATACGCAGGTGAAGAGCGCCGTGCTCGCATTATTCAGGAACTGATAGGGAATATGCGATTGGACCAAATCATGCAAGACCCTTATGGCAATTATGTTATCCAAGCAGCACTCAGTCAATCAAAGGGAACACTTCATTCTAAACTGGTAGAAGCAATAAAACCTCATATGCCAGTTCTCCGGACCAGTCCCTATGGGAAGAAAATCCTCTCTACCAATATTTTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

259

Amino Acids

29.22

Weight (kDa)

8.1

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 57 - 87 3e-06 Pumilio-family RNA binding repeat
PUF PF00806 92 - 126 3.9e-16 Pumilio-family RNA binding repeat
PUF PF00806 165 - 198 4.2e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 545
Acc16I TGCGCA 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 465
AccIII TCCGGA 1 cut(s) 726
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 428
AfeI AGCGCT 1 cut(s) 215
AgsI TTSAA 7 cut(s) 36, 101, 113, 260, 320, 536, 772
AhlI ACTAGT 1 cut(s) 269
AjnI CCWGG 1 cut(s) 163
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
AluBI AGCT 4 cut(s) 263, 286, 442, 448
AluI AGCT 4 cut(s) 263, 286, 442, 448
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 1 cut(s) 398
AlwNI CAGNNNCTG 1 cut(s) 589
Aor13HI TCCGGA 1 cut(s) 726
Aor51HI AGCGCT 1 cut(s) 215
ApeKI GCWGC 4 cut(s) 211, 216, 263, 653
ApoI RAATTY 1 cut(s) 24
AspLEI GCGC 3 cut(s) 211, 216, 567
AspS9I GGNCC 2 cut(s) 610, 729
AsuHPI GGTGA 3 cut(s) 31, 356, 569
AvaII GGWCC 2 cut(s) 610, 729
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 4 cut(s) 203, 223, 250, 665
BccI CCATC 1 cut(s) 335
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 348, 382
BciT130I CCWGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 348
BcuI ACTAGT 1 cut(s) 269
BfaI CTAG 1 cut(s) 270
BfoI RGCGCY 2 cut(s) 217, 568
BfuAI ACCTGC 1 cut(s) 545
BisI GCNGC 4 cut(s) 212, 217, 264, 654
BlsI GCNGC 4 cut(s) 213, 218, 265, 655
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 2 cut(s) 610, 729
BmgT120I GGNCC 2 cut(s) 610, 729
BmrFI CCNGG 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 494
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 141, 164, 452
BsaWI WCCGGW 1 cut(s) 726
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bse1I ACTGG 3 cut(s) 696, 719, 732
BseAI TCCGGA 1 cut(s) 726
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 141, 164, 452
BseMII CTCAG 1 cut(s) 673
BseNI ACTGG 3 cut(s) 696, 719, 732
BseXI GCAGC 4 cut(s) 203, 223, 250, 665
BsgI GTGCAG 1 cut(s) 530
Bsh1285I CGRYCG 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 574
BsiSI CCGG 1 cut(s) 727
BslFI GGGAC 2 cut(s) 100, 720
BsmAI GTCTC 1 cut(s) 348
BsmFI GGGAC 2 cut(s) 100, 720
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 574
Bsp13I TCCGGA 1 cut(s) 726
Bsp143I GATC 3 cut(s) 363, 372, 403
Bsp19I CCATGG 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 672
BspEI TCCGGA 1 cut(s) 726
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 398
BspQI GCTCTTC 1 cut(s) 555
BspTNI GGTCTC 1 cut(s) 348
BsrI ACTGG 3 cut(s) 696, 719, 732
BssECI CCNNGG 3 cut(s) 141, 164, 452
BssMI GATC 3 cut(s) 363, 372, 403
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 299, 463
Bst6I CTCTTC 1 cut(s) 555
BstC8I GCNNGC 3 cut(s) 221, 288, 574
BstDEI CTNAG 2 cut(s) 329, 659
BstDSI CCRYGG 2 cut(s) 141, 452
BstH2I RGCGCY 2 cut(s) 217, 568
BstHHI GCGC 3 cut(s) 211, 216, 567
BstKTI GATC 3 cut(s) 366, 375, 406
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 3 cut(s) 363, 372, 403
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 2 cut(s) 220, 573
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 163
BstV1I GCAGC 4 cut(s) 203, 223, 250, 665
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BtgI CCRYGG 2 cut(s) 141, 452
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 3 cut(s) 221, 288, 574
CaiI CAGNNNCTG 1 cut(s) 589
CfoI GCGC 3 cut(s) 211, 216, 567
Cfr13I GGNCC 2 cut(s) 610, 729
CviAII CATG 4 cut(s) 83, 142, 302, 619
CviJI RGCY 8 cut(s) 95, 163, 263, 286, 306, 442, 448, 543
CviKI_1 RGCY 8 cut(s) 95, 163, 263, 286, 306, 442, 448, 543
DdeI CTNAG 2 cut(s) 329, 659
DpnI GATC 3 cut(s) 365, 374, 405
DpnII GATC 3 cut(s) 363, 372, 403
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
Eco130I CCWWGG 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 610, 729
Eco47III AGCGCT 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 4 cut(s) 86, 145, 305, 622
FaqI GGGAC 2 cut(s) 100, 720
FatI CATG 4 cut(s) 82, 141, 301, 618
FauNDI CATATG 1 cut(s) 714
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 212, 217, 264, 654
Fsp4HI GCNGC 4 cut(s) 212, 217, 264, 654
FspBI CTAG 1 cut(s) 270
FspI TGCGCA 1 cut(s) 210
GlaI GCGC 3 cut(s) 210, 215, 566
GluI GCNGC 4 cut(s) 212, 217, 264, 654
GsuI CTGGAG 1 cut(s) 111
HaeII RGCGCY 2 cut(s) 217, 568
HapII CCGG 1 cut(s) 727
HhaI GCGC 3 cut(s) 211, 216, 567
Hin1II CATG 4 cut(s) 86, 145, 305, 622
Hin6I GCGC 3 cut(s) 209, 214, 565
HinP1I GCGC 3 cut(s) 209, 214, 565
HpaII CCGG 1 cut(s) 727
HphI GGTGA 3 cut(s) 31, 356, 569
Hpy166II GTNNAC 2 cut(s) 175, 466
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 6 cut(s) 51, 128, 245, 473, 584, 727
Hpy8I GTNNAC 2 cut(s) 175, 466
HpyCH4III ACNGT 2 cut(s) 299, 463
HpyCH4IV ACGT 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 573
HpyF3I CTNAG 2 cut(s) 329, 659
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 4 cut(s) 86, 145, 305, 622
HspAI GCGC 3 cut(s) 209, 214, 565
Kpn2I TCCGGA 1 cut(s) 726
Kzo9I GATC 3 cut(s) 363, 372, 403
LguI GCTCTTC 1 cut(s) 555
Lsp1109I GCAGC 4 cut(s) 203, 223, 250, 665
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 204
MalI GATC 3 cut(s) 365, 374, 405
MboI GATC 3 cut(s) 363, 372, 403
MboII GAAGA 3 cut(s) 20, 572, 757
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 574
MluCI AATT 5 cut(s) 24, 274, 428, 490, 637
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 5 cut(s) 128, 163, 478, 720, 764
Mph1103I ATGCAT 1 cut(s) 84
MroI TCCGGA 1 cut(s) 726
MseI TTAA 2 cut(s) 63, 230
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 1 cut(s) 727
MspR9I CCNGG 1 cut(s) 165
MteI GCGCNGCGC 1 cut(s) 212
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 220, 573
NcoI CCATGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 714
NdeII GATC 3 cut(s) 363, 372, 403
NlaIII CATG 4 cut(s) 86, 145, 305, 622
NsbI TGCGCA 1 cut(s) 210
NsiI ATGCAT 1 cut(s) 84
PaqCI CACCTGC 1 cut(s) 545
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PkrI GCNGC 4 cut(s) 213, 218, 265, 655
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspPI GGNCC 2 cut(s) 610, 729
PstNI CAGNNNCTG 1 cut(s) 589
PsuI RGATCY 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 339
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 2 cut(s) 63, 230
SatI GCNGC 4 cut(s) 212, 217, 264, 654
Sau3AI GATC 3 cut(s) 363, 372, 403
Sau96I GGNCC 2 cut(s) 610, 729
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 574
SetI ASST 9 cut(s) 108, 207, 238, 265, 288, 444, 450, 559, 712
SinI GGWCC 2 cut(s) 610, 729
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SpeI ACTAGT 1 cut(s) 269
Sse9I AATT 5 cut(s) 24, 274, 428, 490, 637
SspI AATATT 2 cut(s) 67, 766
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 2 cut(s) 299, 463
TaiI ACGT 1 cut(s) 207
TaqII GACCGA 1 cut(s) 372
TasI AATT 5 cut(s) 24, 274, 428, 490, 637
Tru1I TTAA 2 cut(s) 63, 230
Tru9I TTAA 2 cut(s) 63, 230
TseI GCWGC 4 cut(s) 211, 216, 263, 653
TspDTI ATGAA 3 cut(s) 17, 20, 671
TspGWI ACGGA 1 cut(s) 441
VpaK11BI GGWCC 2 cut(s) 610, 729
XapI RAATTY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 1 cut(s) 270
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.