AT5G56750

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001208
Physical Location & Seq
Forward (+)
22957629 .. 22960916
3288 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56750.1

Sequence Viewer

Length: 1041 bp
ATGACGGACTCTTACGGCGCCGTCTCCGTCGACGTTGGAACAATCTATCTTGGTGGCAAGGAGCATCGTGTTAAAACTGCTAGTGGTGTTGTGTCTGTCATTGTCTATGGAGACAGAGAAAAGCCAGCATTGATTACTTATCCTGATTTAGCCCTAAACCATATGTCATGCTTCCAAGGGTTATTCTTCTGTCCTGAAGCAGCTTCATTGCTGCTTCATAACTTCTGCATTTACCATATCAGTCCACCTGGGCATGAGTTAGGAGCTGCTCCAATTTGTCCTAATGATTCAGTCCCTTCTGCTGAAAACTTGGCAGATCAGATCCTTGAAGTTCTCAACTTTTTCGGCCTTGGTGTTGTGATGTGTATGGGAGTGACTGCAGGTGCTTATATCCTCACCTTATTCGCGATGAAACATAGAGAACGGGTTCTTGGTTTGATTCTTGTCTCACCGTTATGCAAGGCGCCGTCTTGGTCTGAATGGTTTTACAACAAGGTTATCACAAACTTGTTATATTATTACGGAATGTGTGGAGTGGTAAAGGAATTTTTGCTTCAGAGATATTTTAGTAAGGAAGTCCGTGGTAACGTTGAGATTCCAGAGTCAGATATAGCACAAGCTTGCAGAAGACTGCTTGATGAAAGACAAGGCATAAACGTTTTGCGATTTCTTGACGCCATTGACCGGAGACCTGACATCTCAAGTGGATTGAAGAAACTAAAATGCAGGACACTTATCTTCATCGGAGATCAATCTCCTTTCTACTCAGAAGCTGTTCACATGGCAGCAACTTTGGATAGAGGATACTGTGCTTTGGTTGAGGTTCAGGCTTGTGGTTCAATGGTAACAGAGGAGCAACCACATGCAATGTTGATCCCAATGGAATATTTCTTGATGGGTTACGGATTATACAGACCATCTCTTTTCTCCGAGAGCCCTAGAAGTCCACTTAGCCCTTCTTGTATTTCACCTGAGCTTCTCTCTCCTGAAAGCATGGGATTAAAGCTTAAGCCTATCAAAACCAGAATTTCGGCGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0008150 GO:0009914 GO:0010817 GO:0032879 GO:0044424 GO:0044464 GO:0050789 GO:0051049 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

346

Amino Acids

38.3

Weight (kDa)

6.26

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 305 4e-111 Ndr family
Abhydrolase_1 PF00561 63 - 182 2.6e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 371
Acc36I ACCTGC 1 cut(s) 371
AccB1I GGYRCC 2 cut(s) 17, 463
AccI GTMKAC 1 cut(s) 30
AccII CGCG 1 cut(s) 407
AciI CCGC 1 cut(s) 1034
AclI AACGTT 2 cut(s) 588, 657
AclWI GGATC 2 cut(s) 316, 868
AcsI RAATTY 2 cut(s) 545, 1026
AcuI CTGAAG 2 cut(s) 216, 539
AcyI GRCGYC 3 cut(s) 18, 464, 675
AfiI CCNNNNNNNGG 1 cut(s) 684
AflII CTTAAG 1 cut(s) 1007
AgsI TTSAA 3 cut(s) 329, 712, 840
AjnI CCWGG 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 6 cut(s) 203, 266, 620, 773, 976, 1006
AluI AGCT 6 cut(s) 203, 266, 620, 773, 976, 1006
Alw26I GTCTC 4 cut(s) 28, 105, 451, 682
AlwI GGATC 2 cut(s) 316, 868
AlwNI CAGNNNCTG 1 cut(s) 773
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 4 cut(s) 200, 211, 266, 785
ApoI RAATTY 2 cut(s) 545, 1026
Asp700I GAANNNNTTC 2 cut(s) 426, 774
AspLEI GCGC 2 cut(s) 20, 466
AsuHPI GGTGA 3 cut(s) 388, 441, 960
BanI GGYRCC 2 cut(s) 17, 463
BanII GRGCYC 1 cut(s) 938
BbsI GAAGAC 1 cut(s) 634
BbvI GCAGC 4 cut(s) 198, 212, 253, 797
BccI CCATC 2 cut(s) 889, 925
BceAI ACGGC 3 cut(s) 5, 31, 451
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 797
BcoDI GTCTC 4 cut(s) 28, 105, 451, 682
BfaI CTAG 2 cut(s) 81, 939
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 2 cut(s) 21, 467
BfrI CTTAAG 1 cut(s) 1007
BfuAI ACCTGC 1 cut(s) 371
BfuI GTATCC 1 cut(s) 797
BisI GCNGC 5 cut(s) 201, 212, 267, 786, 1035
BlsI GCNGC 5 cut(s) 202, 213, 268, 787, 1036
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 2 cut(s) 19, 465
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 634
BplI GAGNNNNNCTC 2 cut(s) 965, 997
Bpu10I CCTNAGC 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 685
BsaHI GRCGYC 3 cut(s) 18, 464, 675
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 4 cut(s) 175, 248, 349, 580
BsaWI WCCGGW 1 cut(s) 684
Bsc4I CCNNNNNNNGG 1 cut(s) 684
Bse3DI GCAATG 2 cut(s) 206, 873
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 4 cut(s) 175, 248, 349, 580
BseLI CCNNNNNNNGG 1 cut(s) 684
BseMI GCAATG 2 cut(s) 206, 873
BseMII CTCAG 2 cut(s) 780, 963
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 4 cut(s) 198, 212, 253, 797
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 1 cut(s) 348
BshNI GGYRCC 2 cut(s) 17, 463
BsiSI CCGG 1 cut(s) 685
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 684
BsmAI GTCTC 4 cut(s) 28, 105, 451, 682
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 682
Bsp1286I GDGCHC 1 cut(s) 938
Bsp143I GATC 4 cut(s) 316, 321, 748, 873
Bsp68I TCGCGA 1 cut(s) 407
BspACI CCGC 1 cut(s) 1034
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 2 cut(s) 779, 964
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 2 cut(s) 19, 465
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 316, 868
BspT107I GGYRCC 2 cut(s) 17, 463
BspTI CTTAAG 1 cut(s) 1007
BspTNI GGTCTC 1 cut(s) 682
BsrDI GCAATG 2 cut(s) 206, 873
BssECI CCNNGG 4 cut(s) 175, 248, 349, 580
BssMI GATC 4 cut(s) 316, 321, 748, 873
BssNI GRCGYC 3 cut(s) 18, 464, 675
BssT1I CCWWGG 2 cut(s) 175, 349
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 453, 809
BstACI GRCGYC 3 cut(s) 18, 464, 675
BstAFI CTTAAG 1 cut(s) 1007
BstC8I GCNNGC 2 cut(s) 126, 622
BstDEI CTNAG 3 cut(s) 766, 950, 972
BstDSI CCRYGG 1 cut(s) 580
BstFNI CGCG 1 cut(s) 407
BstH2I RGCGCY 2 cut(s) 21, 467
BstHHI GCGC 2 cut(s) 20, 466
BstKTI GATC 4 cut(s) 319, 324, 751, 876
BstMAI GTCTC 4 cut(s) 28, 105, 451, 682
BstMBI GATC 4 cut(s) 316, 321, 748, 873
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 4 cut(s) 198, 212, 253, 797
BstV2I GAAGAC 1 cut(s) 634
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 580
BtgZI GCGATG 1 cut(s) 422
BtuMI TCGCGA 1 cut(s) 407
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 126, 622
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 2 cut(s) 20, 466
CseI GACGC 1 cut(s) 683
CviAII CATG 5 cut(s) 168, 254, 781, 863, 994
DdeI CTNAG 3 cut(s) 766, 950, 972
DinI GGCGCC 2 cut(s) 19, 465
DpnI GATC 4 cut(s) 318, 323, 750, 875
DpnII GATC 4 cut(s) 316, 321, 748, 873
Eco130I CCWWGG 2 cut(s) 175, 349
Eco24I GRGCYC 1 cut(s) 938
Eco31I GGTCTC 1 cut(s) 682
Eco57I CTGAAG 2 cut(s) 216, 539
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 175, 349
EcoT38I GRGCYC 1 cut(s) 938
EgeI GGCGCC 2 cut(s) 19, 465
EheI GGCGCC 2 cut(s) 19, 465
ErhI CCWWGG 2 cut(s) 175, 349
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 5 cut(s) 171, 257, 784, 866, 997
FaqI GGGAC 1 cut(s) 278
FatI CATG 5 cut(s) 167, 253, 780, 862, 993
FauNDI CATATG 1 cut(s) 162
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 5 cut(s) 201, 212, 267, 786, 1035
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 5 cut(s) 201, 212, 267, 786, 1035
FspBI CTAG 2 cut(s) 81, 939
GlaI GCGC 2 cut(s) 19, 465
GluI GCNGC 5 cut(s) 201, 212, 267, 786, 1035
HaeII RGCGCY 2 cut(s) 21, 467
HaeIII GGCC 1 cut(s) 348
HapII CCGG 1 cut(s) 685
HgaI GACGC 1 cut(s) 683
HhaI GCGC 2 cut(s) 20, 466
Hin1I GRCGYC 3 cut(s) 18, 464, 675
Hin1II CATG 5 cut(s) 171, 257, 784, 866, 997
Hin6I GCGC 2 cut(s) 18, 464
HinP1I GCGC 2 cut(s) 18, 464
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 2 cut(s) 618, 1004
HinfI GANTC 5 cut(s) 8, 287, 439, 595, 602
HpaII CCGG 1 cut(s) 685
HphI GGTGA 3 cut(s) 388, 441, 960
Hpy166II GTNNAC 4 cut(s) 31, 245, 778, 947
Hpy188I TCNGA 7 cut(s) 321, 478, 558, 607, 746, 769, 931
Hpy188III TCNNGA 7 cut(s) 143, 194, 406, 599, 671, 892, 986
Hpy8I GTNNAC 4 cut(s) 31, 245, 778, 947
Hpy99I CGWCG 2 cut(s) 32, 35
HpyAV CCTTC 2 cut(s) 306, 966
HpyCH4III ACNGT 2 cut(s) 453, 809
HpyCH4IV ACGT 3 cut(s) 33, 588, 657
HpyCH4V TGCA 6 cut(s) 228, 380, 459, 624, 726, 866
HpyF3I CTNAG 3 cut(s) 766, 950, 972
HpySE526I ACGT 3 cut(s) 33, 588, 657
Hsp92I GRCGYC 3 cut(s) 18, 464, 675
Hsp92II CATG 5 cut(s) 171, 257, 784, 866, 997
HspAI GCGC 2 cut(s) 18, 464
KasI GGCGCC 2 cut(s) 17, 463
Kzo9I GATC 4 cut(s) 316, 321, 748, 873
LmnI GCTCC 4 cut(s) 61, 263, 274, 853
Lsp1109I GCAGC 4 cut(s) 198, 212, 253, 797
LweI GCATC 1 cut(s) 73
MaeI CTAG 2 cut(s) 81, 939
MaeII ACGT 3 cut(s) 33, 588, 657
MaeIII GTNAC 4 cut(s) 373, 584, 844, 899
MalI GATC 4 cut(s) 318, 323, 750, 875
MboI GATC 4 cut(s) 316, 321, 748, 873
MboII GAAGA 4 cut(s) 178, 639, 724, 730
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 1 cut(s) 938
MluCI AATT 3 cut(s) 273, 545, 1026
Mly113I GGCGCC 2 cut(s) 18, 464
MlyI GAGTC 2 cut(s) 2, 611
MmeI TCCRAC 1 cut(s) 16
MnlI CCTC 4 cut(s) 404, 794, 814, 844
MroXI GAANNNNTTC 2 cut(s) 426, 774
MseI TTAA 4 cut(s) 72, 1001, 1008, 1039
MslI CAYNNNNRTG 1 cut(s) 454
MspCI CTTAAG 1 cut(s) 1007
MspI CCGG 1 cut(s) 685
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 407
NarI GGCGCC 2 cut(s) 18, 464
NdeI CATATG 1 cut(s) 162
NdeII GATC 4 cut(s) 316, 321, 748, 873
NlaIII CATG 5 cut(s) 171, 257, 784, 866, 997
NlaIV GGNNCC 2 cut(s) 19, 465
NmuCI GTSAC 1 cut(s) 373
NruI TCGCGA 1 cut(s) 407
NspI RCATGY 1 cut(s) 866
PaqCI CACCTGC 1 cut(s) 371
PdmI GAANNNNTTC 2 cut(s) 426, 774
PfeI GAWTC 3 cut(s) 287, 439, 595
PkrI GCNGC 5 cut(s) 202, 213, 268, 787, 1036
PleI GAGTC 2 cut(s) 2, 610
PluTI GGCGCC 2 cut(s) 21, 467
PpsI GAGTC 2 cut(s) 2, 610
Psp1406I AACGTT 2 cut(s) 588, 657
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 19, 465
PstI CTGCAG 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 321
RruI TCGCGA 1 cut(s) 407
RseI CAYNNNNRTG 1 cut(s) 454
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 4 cut(s) 72, 1001, 1008, 1039
SatI GCNGC 5 cut(s) 201, 212, 267, 786, 1035
Sau3AI GATC 4 cut(s) 316, 321, 748, 873
SchI GAGTC 2 cut(s) 2, 611
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 938
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 2 cut(s) 19, 465
SgrDI CGTCGACG 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 454
SmlI CTYRAG 2 cut(s) 700, 1007
SmoI CTYRAG 2 cut(s) 700, 1007
Sse9I AATT 3 cut(s) 273, 545, 1026
SsiI CCGC 1 cut(s) 1034
SspDI GGCGCC 2 cut(s) 17, 463
SspI AATATT 1 cut(s) 887
SspMI CTAG 2 cut(s) 81, 939
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 2 cut(s) 175, 349
TaaI ACNGT 2 cut(s) 453, 809
TaiI ACGT 3 cut(s) 36, 591, 660
TaqI TCGA 1 cut(s) 30
TasI AATT 3 cut(s) 273, 545, 1026
TauI GCSGC 1 cut(s) 1037
TfiI GAWTC 3 cut(s) 287, 439, 595
Tru1I TTAA 4 cut(s) 72, 1001, 1008, 1039
Tru9I TTAA 4 cut(s) 72, 1001, 1008, 1039
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 4 cut(s) 200, 211, 266, 785
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 5 cut(s) 195, 206, 425, 654, 730
TspGWI ACGGA 5 cut(s) 16, 20, 537, 569, 918
Vha464I CTTAAG 1 cut(s) 1007
XapI RAATTY 2 cut(s) 545, 1026
XceI RCATGY 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 2 cut(s) 426, 774
XspI CTAG 2 cut(s) 81, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.