Rorug02G0103300

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001208
Physical Location & Seq
Forward (+)
8821260 .. 8821979
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0103300.1

Sequence Viewer

Length: 720 bp
ATGTCTACGGCTCAAGACTCCACAGTAGTGATTGGATATCCAGCTGGGGCGTATCAAGTCGACACCGGCTACCCTTACCCTCCATCCCCTGCCCGCTCAAACTACGACCAACCCACGAGGTCGGACCAGCGCACTAAGTCCGTCAACCGCGCAGTAGCTATATTACAAGGCGTCACTCTTGGAGTCTTCATCATAGGGATGGCTCTTCTGGCCTTGAGACGGAAAGACCCCGAGTTCAAGCTCGAGTCGGCGACGGTGTCTCGACTCAACGCCACTGGCCCCGAGTTAACGGCCACGTTGAACATCACTCTCCTCATCACCAACCCCGACCACAGATACAGAGTCTCCTTCAAAACCCTCCAGGCCAGGCTCCTCTACCGGGACCACAGCCGTCCCATGCTGCAGGCTGCGACGCCGTTGCCTCCCTTAGTTGTGAACAAGAGGAACCAGACACGTGTCGATATGAAGCTCGCGGCGGTGAGCCAGTGGGTCGGCGGTGACGTGGGAAAGGAAATCTACGACGACAGGAGTCGGGGGTTTTTGAGTTTTCGACTTAGGGTGAGGGCTTGCTCTGGACATGATGTCCACAACTTTGTTACAGGCTGGTGTTTTCGGTTCGACTGTGATCGGGTTTATTTCGGGTTTTACCATAATGAGACGGGCACGTTGATCGAACCAAGAAAATGCGATTTATTGATTGGCCCAAAGTCAAATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

239

Amino Acids

26.85

Weight (kDa)

9.63

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 2 cut(s) 5, 60
AccII CGCG 2 cut(s) 150, 473
AciI CCGC 5 cut(s) 94, 148, 473, 476, 495
AcoI YGGCCR 1 cut(s) 291
AcvI CACGTG 1 cut(s) 455
AcyI GRCGYC 2 cut(s) 171, 413
AfiI CCNNNNNNNGG 2 cut(s) 219, 379
AflIII ACRYGT 2 cut(s) 452, 454
AgsI TTSAA 3 cut(s) 238, 301, 352
AhdI GACNNNNNGTC 1 cut(s) 581
AjiI CACGTC 1 cut(s) 502
AjnI CCWGG 2 cut(s) 360, 365
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 4 cut(s) 44, 158, 241, 469
AluI AGCT 4 cut(s) 44, 158, 241, 469
Alw26I GTCTC 4 cut(s) 211, 264, 349, 650
Ama87I CYCGRG 3 cut(s) 230, 242, 281
AoxI GGCC 5 cut(s) 210, 277, 291, 363, 700
ApeKI GCWGC 2 cut(s) 400, 407
AspLEI GCGC 2 cut(s) 132, 152
AspS9I GGNCC 4 cut(s) 124, 278, 382, 701
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 4 cut(s) 310, 490, 509, 571
AvaI CYCGRG 3 cut(s) 230, 242, 281
AvaII GGWCC 2 cut(s) 124, 382
BaeGI GKGCMC 1 cut(s) 665
BauI CACGAG 1 cut(s) 115
BbrPI CACGTG 1 cut(s) 455
BbsI GAAGAC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 387, 394
BccI CCATC 2 cut(s) 91, 193
BceAI ACGGC 4 cut(s) 24, 306, 375, 400
BcgI CGANNNNNNTGC 4 cut(s) 400, 434, 652, 686
BciT130I CCWGG 2 cut(s) 362, 367
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 4 cut(s) 211, 264, 349, 650
BfmI CTRYAG 1 cut(s) 401
BisI GCNGC 3 cut(s) 401, 408, 474
BlsI GCNGC 3 cut(s) 402, 409, 475
Bme1390I CCNGG 3 cut(s) 362, 367, 380
Bme18I GGWCC 2 cut(s) 124, 382
BmeRI GACNNNNNGTC 1 cut(s) 581
BmeT110I CYCGRG 3 cut(s) 230, 242, 281
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 4 cut(s) 124, 278, 382, 701
BmiI GGNNCC 4 cut(s) 280, 371, 383, 446
BmrFI CCNGG 3 cut(s) 362, 367, 380
BoxI GACNNNNGTC 2 cut(s) 455, 528
BpiI GAAGAC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 235
BpuMI CCSGG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 455
BsaHI GRCGYC 2 cut(s) 171, 413
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 379
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 280, 484
BseBI CCWGG 2 cut(s) 362, 367
BseGI GGATG 2 cut(s) 83, 204
BseLI CCNNNNNNNGG 2 cut(s) 219, 379
BseNI ACTGG 2 cut(s) 280, 484
BseRI GAGGAG 2 cut(s) 302, 362
BseSI GKGCMC 1 cut(s) 665
BseXI GCAGC 2 cut(s) 387, 394
BseYI CCCAGC 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 150, 473
BshFI GGCC 5 cut(s) 212, 279, 293, 365, 702
BsiHKCI CYCGRG 3 cut(s) 230, 242, 281
BsiSI CCGG 2 cut(s) 66, 379
BslFI GGGAC 2 cut(s) 378, 395
BslI CCNNNNNNNGG 2 cut(s) 219, 379
BsmAI GTCTC 4 cut(s) 211, 264, 349, 650
BsmBI CGTCTC 2 cut(s) 211, 650
BsmFI GGGAC 2 cut(s) 378, 395
BsnI GGCC 5 cut(s) 212, 279, 293, 365, 702
BsoBI CYCGRG 3 cut(s) 230, 242, 281
Bsp1286I GDGCHC 1 cut(s) 665
Bsp143I GATC 2 cut(s) 625, 669
BspACI CCGC 5 cut(s) 94, 148, 473, 476, 495
BspANI GGCC 5 cut(s) 212, 279, 293, 365, 702
BspFNI CGCG 2 cut(s) 150, 473
BspLI GGNNCC 4 cut(s) 280, 371, 383, 446
BspMAI CTGCAG 1 cut(s) 405
BspQI GCTCTTC 1 cut(s) 210
BsrBI CCGCTC 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 2 cut(s) 280, 484
BssAI RCCGGY 1 cut(s) 65
BssMI GATC 2 cut(s) 625, 669
BssNI GRCGYC 2 cut(s) 171, 413
BssSI CACGAG 1 cut(s) 115
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 2 cut(s) 362, 367
Bst4CI ACNGT 3 cut(s) 25, 256, 623
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 2 cut(s) 171, 413
BstBAI YACGTR 1 cut(s) 455
BstC8I GCNNGC 4 cut(s) 94, 405, 471, 568
BstDEI CTNAG 3 cut(s) 135, 427, 554
BstF5I GGATG 2 cut(s) 83, 204
BstFNI CGCG 2 cut(s) 150, 473
BstHHI GCGC 2 cut(s) 132, 152
BstKTI GATC 2 cut(s) 628, 672
BstMAI GTCTC 4 cut(s) 211, 264, 349, 650
BstMBI GATC 2 cut(s) 625, 669
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 362, 367
BstPAI GACNNNNGTC 2 cut(s) 455, 528
BstSCI CCNGG 3 cut(s) 360, 365, 378
BstSFI CTRYAG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 665
BstUI CGCG 2 cut(s) 150, 473
BstV1I GCAGC 2 cut(s) 387, 394
BstV2I GAAGAC 1 cut(s) 178
BsuRI GGCC 5 cut(s) 212, 279, 293, 365, 702
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 2 cut(s) 83, 204
BtsIMutI CAGTG 3 cut(s) 273, 491, 715
Cac8I GCNNGC 4 cut(s) 94, 405, 471, 568
CfoI GCGC 2 cut(s) 132, 152
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 4 cut(s) 124, 278, 382, 701
CseI GACGC 2 cut(s) 160, 421
CviAII CATG 2 cut(s) 397, 578
DdeI CTNAG 3 cut(s) 135, 427, 554
DpnI GATC 2 cut(s) 627, 671
DpnII GATC 2 cut(s) 625, 669
DriI GACNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 210
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 124, 382
Eco72I CACGTG 1 cut(s) 455
Eco88I CYCGRG 3 cut(s) 230, 242, 281
EcoRII CCWGG 2 cut(s) 360, 365
EcoRV GATATC 1 cut(s) 38
Esp3I CGTCTC 2 cut(s) 211, 650
FaeI CATG 2 cut(s) 400, 581
FaiI YATR 6 cut(s) 161, 194, 398, 464, 579, 651
FaqI GGGAC 2 cut(s) 378, 395
FatI CATG 2 cut(s) 396, 577
FauI CCCGC 1 cut(s) 101
FblI GTMKAC 2 cut(s) 5, 60
Fnu4HI GCNGC 3 cut(s) 401, 408, 474
FokI GGATG 2 cut(s) 70, 211
Fsp4HI GCNGC 3 cut(s) 401, 408, 474
GlaI GCGC 2 cut(s) 131, 151
GluI GCNGC 3 cut(s) 401, 408, 474
GsaI CCCAGC 1 cut(s) 48
GsuI CTGGAG 1 cut(s) 344
HaeIII GGCC 5 cut(s) 212, 279, 293, 365, 702
HapII CCGG 2 cut(s) 66, 379
HgaI GACGC 2 cut(s) 160, 421
HhaI GCGC 2 cut(s) 132, 152
Hin1I GRCGYC 2 cut(s) 171, 413
Hin1II CATG 2 cut(s) 400, 581
Hin6I GCGC 2 cut(s) 130, 150
HinP1I GCGC 2 cut(s) 130, 150
HincII GTYRAC 3 cut(s) 61, 145, 288
HindII GTYRAC 3 cut(s) 61, 145, 288
HinfI GANTC 6 cut(s) 17, 183, 245, 264, 342, 529
HpaI GTTAAC 1 cut(s) 288
HpaII CCGG 2 cut(s) 66, 379
HphI GGTGA 4 cut(s) 310, 490, 509, 571
Hpy166II GTNNAC 6 cut(s) 6, 61, 145, 288, 436, 586
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 14, 261, 573
Hpy8I GTNNAC 6 cut(s) 6, 61, 145, 288, 436, 586
Hpy99I CGWCG 3 cut(s) 256, 415, 524
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 3 cut(s) 25, 256, 623
HpyCH4IV ACGT 4 cut(s) 296, 454, 501, 665
HpyCH4V TGCA 1 cut(s) 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 3 cut(s) 135, 427, 554
HpySE526I ACGT 4 cut(s) 296, 454, 501, 665
Hsp92I GRCGYC 2 cut(s) 171, 413
Hsp92II CATG 2 cut(s) 400, 581
HspAI GCGC 2 cut(s) 130, 150
KspAI GTTAAC 1 cut(s) 288
Kzo9I GATC 2 cut(s) 625, 669
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 375
Lsp1109I GCAGC 2 cut(s) 387, 394
MaeII ACGT 4 cut(s) 296, 454, 501, 665
MaeIII GTNAC 3 cut(s) 172, 497, 595
MalI GATC 2 cut(s) 627, 671
MbiI CCGCTC 1 cut(s) 96
MboI GATC 2 cut(s) 625, 669
MboII GAAGA 2 cut(s) 178, 197
MhlI GDGCHC 1 cut(s) 665
MlyI GAGTC 6 cut(s) 11, 192, 254, 258, 351, 538
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 8 cut(s) 90, 111, 323, 368, 383, 432, 435, 555
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 2 cut(s) 26, 197
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 2 cut(s) 66, 379
MspR9I CCNGG 3 cut(s) 362, 367, 380
MvaI CCWGG 2 cut(s) 362, 367
MvnI CGCG 2 cut(s) 150, 473
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 1 cut(s) 380
NdeII GATC 2 cut(s) 625, 669
NlaIII CATG 2 cut(s) 400, 581
NlaIV GGNNCC 4 cut(s) 280, 371, 383, 446
NmuCI GTSAC 2 cut(s) 172, 497
OliI CACNNNNGTG 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PflFI GACNNNGTC 1 cut(s) 256
PkrI GCNGC 3 cut(s) 402, 409, 475
PleI GAGTC 6 cut(s) 11, 191, 253, 258, 350, 537
PmaCI CACGTG 1 cut(s) 455
PmlI CACGTG 1 cut(s) 455
PpsI GAGTC 6 cut(s) 11, 191, 253, 258, 350, 537
Ppu21I YACGTR 1 cut(s) 455
PshAI GACNNNNGTC 2 cut(s) 455, 528
Psp6I CCWGG 2 cut(s) 360, 365
PspCI CACGTG 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 2 cut(s) 360, 365
PspN4I GGNNCC 4 cut(s) 280, 371, 383, 446
PspPI GGNCC 4 cut(s) 124, 278, 382, 701
PspXI VCTCGAGB 1 cut(s) 242
PstI CTGCAG 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 44
RseI CAYNNNNRTG 2 cut(s) 26, 197
SalI GTCGAC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 3 cut(s) 401, 408, 474
Sau3AI GATC 2 cut(s) 625, 669
Sau96I GGNCC 4 cut(s) 124, 278, 382, 701
SchI GAGTC 6 cut(s) 11, 192, 254, 258, 351, 538
ScrFI CCNGG 3 cut(s) 362, 367, 380
SduI GDGCHC 1 cut(s) 665
SetI ASST 9 cut(s) 46, 122, 160, 243, 299, 457, 471, 504, 668
SfcI CTRYAG 1 cut(s) 401
Sfr274I CTCGAG 1 cut(s) 242
SinI GGWCC 2 cut(s) 124, 382
SlaI CTCGAG 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 26, 197
SmlI CTYRAG 3 cut(s) 12, 214, 242
SmoI CTYRAG 3 cut(s) 12, 214, 242
SsiI CCGC 5 cut(s) 94, 148, 473, 476, 495
StyD4I CCNGG 3 cut(s) 360, 365, 378
TaaI ACNGT 3 cut(s) 25, 256, 623
TaiI ACGT 4 cut(s) 299, 457, 504, 668
TaqI TCGA 7 cut(s) 60, 243, 262, 459, 550, 618, 672
TauI GCSGC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TscAI CASTG 2 cut(s) 280, 491
TseFI GTSAC 2 cut(s) 172, 497
TseI GCWGC 2 cut(s) 400, 407
Tsp45I GTSAC 2 cut(s) 172, 497
TspDTI ATGAA 2 cut(s) 178, 479
TspGWI ACGGA 2 cut(s) 130, 235
TspRI CASTG 2 cut(s) 280, 491
Tth111I GACNNNGTC 1 cut(s) 256
VpaK11BI GGWCC 2 cut(s) 124, 382
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 2 cut(s) 5, 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.