pycom02g05910

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001208
Physical Location & Seq
Reverse (-)
3980820 .. 3984316
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05910.7

Sequence Viewer

Length: 1044 bp
ATGGCTGAACCAAACGACGCCGTCTCCCTCGATATGGAGAAGATCGATCTCGGTGGAAAGGAACATTTTATTCGAACTGGCCGTGGTTCTGTATCCGTTATCGTGTATGGTGACCAAGAGAAGCCTTCACTTATCACTTATCCTGATTTAGCTCTAAATCATGTATCTTGTTTCCAAGGGTTATTCTTTTGTCCGGAAGCGGCTTCTTTGCTGCTCCACAACTTCTGCATATATCATATCAGTCCTCCTGGCCATGAGTTAGGAGCTGCTTCAATTTGTCCAGATGATCCTGTGCCTTCAGTTGATGACTTGGCAGATCAGATCCTTGAGGTTCTCAATTTTTTTGGGCTTGGGGCGGTTATGTGCATGGGGGTGACAGCGGGTGCTTATATCCTCTCTCTTTTTGCTATGAAATATAGGGAGCGTGTCCTTGGATTGATACTTGTATCCCCTTTATGCAAAGAACCCTCTTGGACAGAATGGTTTTATAATAAGGTGATGTCGAATATGCTATATTTCTATGGCATGTGTGGCTTGCTCAAAGAGTGTTTGCTTCAGCGCTACTTCAGTAAGGATGTTCGTGGTAGTGTTGAAGTTCCAGAGTCAGATATAGTTCAAGCGTGCAGAAAATTGCTGGAAGAGAGGCAGAGCTTAAACGTTTGGAGATTTCTTCAAGCAATCAATCGGAGACCTGACATAACCGAAGGGTTAAAATCGCTAAGGTGTCGCACGCTTATTTTTGTTGGGGATAGCTCTCCCTTCCATTCTGAGGCTCTCCACATGACCTCAAAGTTGGACAGGAGATATAGTGCCTTAGTAGAGGTCCAGGCTTGTGGATCGATGGTAACAGAGGAGCAGCCACATGCAATGTTGATACCAATGGAATACTTCTTCATGGGGTATGGCCTGTACCGGCCATGCCATTTCAGCGACAGCCCTAGGAGCCCGCTCAGCCCATCTTGCATCTCCCCGGAGCTTCTCTCCCCAGAAAGCATGGGCTTGAAACTAAAACCAATAAAGACCCGTGTTTCACTCAGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

348

Amino Acids

38.92

Weight (kDa)

5.75

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AccBSI CCGCTC 1 cut(s) 949
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 4 cut(s) 200, 356, 380, 947
AclI AACGTT 1 cut(s) 657
AclWI GGATC 3 cut(s) 281, 316, 844
AcoI YGGCCR 3 cut(s) 79, 250, 914
AcuI CTGAAG 3 cut(s) 282, 539, 550
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 1 cut(s) 911
AfeI AGCGCT 1 cut(s) 560
AfiI CCNNNNNNNGG 2 cut(s) 34, 769
AgsI TTSAA 5 cut(s) 273, 593, 617, 674, 1003
AjnI CCWGG 2 cut(s) 247, 825
AluBI AGCT 5 cut(s) 152, 266, 651, 753, 976
AluI AGCT 5 cut(s) 152, 266, 651, 753, 976
Alw26I GTCTC 2 cut(s) 28, 682
AlwI GGATC 3 cut(s) 281, 316, 844
Aor13HI TCCGGA 1 cut(s) 193
Aor51HI AGCGCT 1 cut(s) 560
AoxI GGCC 4 cut(s) 79, 250, 904, 914
ApeKI GCWGC 3 cut(s) 211, 266, 856
AspA2I CCTAGG 1 cut(s) 938
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 1 cut(s) 823
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 3 cut(s) 122, 385, 508
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 823
AvrII CCTAGG 1 cut(s) 938
BalI TGGCCA 1 cut(s) 252
BanII GRGCYC 1 cut(s) 947
BbvI GCAGC 3 cut(s) 198, 253, 868
BccI CCATC 2 cut(s) 835, 964
BceAI ACGGC 2 cut(s) 5, 66
BciT130I CCWGG 2 cut(s) 249, 827
BciVI GTATCC 2 cut(s) 103, 457
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 2 cut(s) 28, 682
BfaI CTAG 1 cut(s) 939
BfoI RGCGCY 1 cut(s) 562
BfuI GTATCC 2 cut(s) 103, 457
BisI GCNGC 4 cut(s) 201, 212, 267, 857
BlnI CCTAGG 1 cut(s) 938
BlpI GCTNAGC 1 cut(s) 950
BlsI GCNGC 4 cut(s) 202, 213, 268, 858
Bme1390I CCNGG 3 cut(s) 249, 827, 971
Bme18I GGWCC 1 cut(s) 823
BmgT120I GGNCC 1 cut(s) 823
BmiI GGNNCC 1 cut(s) 944
BmrFI CCNGG 3 cut(s) 249, 827, 971
BmsI GCATC 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 965, 997
Bpu10I CCTNAGC 1 cut(s) 719
Bpu1102I GCTNAGC 1 cut(s) 950
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 971
Bsa29I ATCGAT 2 cut(s) 45, 839
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 5 cut(s) 82, 175, 430, 938, 969
BsaWI WCCGGW 1 cut(s) 193
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 793, 823
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 769
Bse118I RCCGGY 1 cut(s) 912
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 873
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 2 cut(s) 249, 827
BseCI ATCGAT 2 cut(s) 45, 839
BseDI CCNNGG 5 cut(s) 82, 175, 430, 938, 969
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 2 cut(s) 34, 769
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 2 cut(s) 759, 964
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 198, 253, 868
BsgI GTGCAG 1 cut(s) 643
BshFI GGCC 4 cut(s) 81, 252, 906, 916
BshVI ATCGAT 2 cut(s) 45, 839
BsiSI CCGG 3 cut(s) 194, 913, 971
BslI CCNNNNNNNGG 2 cut(s) 34, 769
BsmAI GTCTC 2 cut(s) 28, 682
BsmBI CGTCTC 1 cut(s) 28
BsnI GGCC 4 cut(s) 81, 252, 906, 916
Bso31I GGTCTC 1 cut(s) 682
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 947
Bsp13I TCCGGA 1 cut(s) 193
Bsp143I GATC 6 cut(s) 42, 46, 286, 316, 321, 836
Bsp1720I GCTNAGC 1 cut(s) 950
BspACI CCGC 4 cut(s) 200, 356, 380, 947
BspANI GGCC 4 cut(s) 81, 252, 906, 916
BspCNI CTCAG 2 cut(s) 760, 963
BspDI ATCGAT 2 cut(s) 45, 839
BspEI TCCGGA 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 944
BspPI GGATC 3 cut(s) 281, 316, 844
BspT104I TTCGAA 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 682
BsrBI CCGCTC 1 cut(s) 949
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 912
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 912
BssECI CCNNGG 5 cut(s) 82, 175, 430, 938, 969
BssMI GATC 6 cut(s) 42, 46, 286, 316, 321, 836
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 175, 430, 938
Bst2UI CCWGG 2 cut(s) 249, 827
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 18
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 4 cut(s) 536, 622, 731, 947
BstDEI CTNAG 5 cut(s) 719, 768, 814, 950, 1034
BstDSI CCRYGG 1 cut(s) 82
BstEII GGTNACC 1 cut(s) 110
BstF5I GGATG 1 cut(s) 580
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 6 cut(s) 45, 49, 289, 319, 324, 839
BstMAI GTCTC 2 cut(s) 28, 682
BstMBI GATC 6 cut(s) 42, 46, 286, 316, 321, 836
BstMWI GCNNNNNNNGC 5 cut(s) 531, 927, 942, 951, 960
BstNI CCWGG 2 cut(s) 249, 827
BstNSI RCATGY 2 cut(s) 529, 866
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 3 cut(s) 247, 825, 969
BstV1I GCAGC 3 cut(s) 198, 253, 868
BstX2I RGATCY 1 cut(s) 321
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 321
Bsu15I ATCGAT 2 cut(s) 45, 839
BsuI GTATCC 2 cut(s) 103, 457
BsuRI GGCC 4 cut(s) 81, 252, 906, 916
BsuTUI ATCGAT 2 cut(s) 45, 839
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 580
Cac8I GCNNGC 4 cut(s) 536, 622, 731, 947
CfoI GCGC 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 912
Cfr13I GGNCC 1 cut(s) 823
ClaI ATCGAT 2 cut(s) 45, 839
CseI GACGC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 910
CviAII CATG 9 cut(s) 161, 254, 367, 526, 781, 863, 895, 918, 994
CviQI GTAC 1 cut(s) 910
DdeI CTNAG 5 cut(s) 719, 768, 814, 950, 1034
DpnI GATC 6 cut(s) 44, 48, 288, 318, 323, 838
DpnII GATC 6 cut(s) 42, 46, 286, 316, 321, 836
EaeI YGGCCR 3 cut(s) 79, 250, 914
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Eco130I CCWWGG 3 cut(s) 175, 430, 938
Eco24I GRGCYC 1 cut(s) 947
Eco31I GGTCTC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 823
Eco47III AGCGCT 1 cut(s) 560
Eco57I CTGAAG 3 cut(s) 282, 539, 550
Eco91I GGTNACC 1 cut(s) 110
EcoO65I GGTNACC 1 cut(s) 110
EcoRII CCWGG 2 cut(s) 247, 825
EcoT14I CCWWGG 3 cut(s) 175, 430, 938
EcoT38I GRGCYC 1 cut(s) 947
ErhI CCWWGG 3 cut(s) 175, 430, 938
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 9 cut(s) 164, 257, 370, 529, 784, 866, 898, 921, 997
FatI CATG 9 cut(s) 160, 253, 366, 525, 780, 862, 894, 917, 993
FauI CCCGC 2 cut(s) 373, 954
Fnu4HI GCNGC 4 cut(s) 201, 212, 267, 857
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 947
Fsp4HI GCNGC 4 cut(s) 201, 212, 267, 857
FspBI CTAG 1 cut(s) 939
GlaI GCGC 1 cut(s) 560
GluI GCNGC 4 cut(s) 201, 212, 267, 857
HaeII RGCGCY 1 cut(s) 562
HaeIII GGCC 4 cut(s) 81, 252, 906, 916
HapII CCGG 3 cut(s) 194, 913, 971
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 561
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 9 cut(s) 164, 257, 370, 529, 784, 866, 898, 921, 997
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HinfI GANTC 1 cut(s) 602
HpaII CCGG 3 cut(s) 194, 913, 971
HphI GGTGA 3 cut(s) 122, 385, 508
Hpy188I TCNGA 4 cut(s) 321, 607, 687, 769
Hpy188III TCNNGA 4 cut(s) 143, 194, 281, 599
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 135, 306, 698, 769
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 6 cut(s) 228, 366, 459, 624, 866, 963
HpyF10VI GCNNNNNNNGC 5 cut(s) 531, 927, 942, 951, 960
HpyF3I CTNAG 5 cut(s) 719, 768, 814, 950, 1034
HpySE526I ACGT 1 cut(s) 657
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 9 cut(s) 164, 257, 370, 529, 784, 866, 898, 921, 997
HspAI GCGC 1 cut(s) 559
Kpn2I TCCGGA 1 cut(s) 193
Kzo9I GATC 6 cut(s) 42, 46, 286, 316, 321, 836
LmnI GCTCC 6 cut(s) 219, 263, 421, 853, 942, 973
Lsp1109I GCAGC 3 cut(s) 198, 253, 868
LweI GCATC 1 cut(s) 972
MaeI CTAG 1 cut(s) 939
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 3 cut(s) 110, 373, 844
MalI GATC 6 cut(s) 44, 48, 288, 318, 323, 838
MbiI CCGCTC 1 cut(s) 949
MboI GATC 6 cut(s) 42, 46, 286, 316, 321, 836
MboII GAAGA 4 cut(s) 52, 650, 662, 883
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 1 cut(s) 947
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 3 cut(s) 273, 337, 629
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 193
MscI TGGCCA 1 cut(s) 252
MseI TTAA 2 cut(s) 653, 710
MslI CAYNNNNRTG 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 3 cut(s) 194, 913, 971
MspR9I CCNGG 3 cut(s) 249, 827, 971
MvaI CCWGG 2 cut(s) 249, 827
MwoI GCNNNNNNNGC 5 cut(s) 531, 927, 942, 951, 960
NciI CCSGG 1 cut(s) 971
NdeII GATC 6 cut(s) 42, 46, 286, 316, 321, 836
NlaIII CATG 9 cut(s) 164, 257, 370, 529, 784, 866, 898, 921, 997
NlaIV GGNNCC 1 cut(s) 944
NmuCI GTSAC 2 cut(s) 110, 373
NspI RCATGY 2 cut(s) 529, 866
NspV TTCGAA 1 cut(s) 73
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 4 cut(s) 202, 213, 268, 858
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PsiI TTATAA 1 cut(s) 489
Psp1406I AACGTT 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 247, 825
PspEI GGTNACC 1 cut(s) 110
PspGI CCWGG 2 cut(s) 247, 825
PspN4I GGNNCC 1 cut(s) 944
PspPI GGNCC 1 cut(s) 823
PsuI RGATCY 1 cut(s) 321
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 1 cut(s) 911
RsaNI GTAC 1 cut(s) 910
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 2 cut(s) 653, 710
SatI GCNGC 4 cut(s) 201, 212, 267, 857
Sau3AI GATC 6 cut(s) 42, 46, 286, 316, 321, 836
Sau96I GGNCC 1 cut(s) 823
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 249, 827, 971
SduI GDGCHC 1 cut(s) 947
SfaNI GCATC 1 cut(s) 972
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 3 cut(s) 273, 337, 629
SsiI CCGC 4 cut(s) 200, 356, 380, 947
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 3 cut(s) 247, 825, 969
StyI CCWWGG 3 cut(s) 175, 430, 938
TaiI ACGT 1 cut(s) 660
TaqI TCGA 5 cut(s) 30, 45, 73, 503, 839
TasI AATT 3 cut(s) 273, 337, 629
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 2 cut(s) 653, 710
Tru9I TTAA 2 cut(s) 653, 710
TseFI GTSAC 2 cut(s) 110, 373
TseI GCWGC 3 cut(s) 211, 266, 856
Tsp45I GTSAC 2 cut(s) 110, 373
TspDTI ATGAA 2 cut(s) 425, 883
TspGWI ACGGA 1 cut(s) 85
Tth111I GACNNNGTC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 823
XceI RCATGY 2 cut(s) 529, 866
XmaJI CCTAGG 1 cut(s) 938
XspI CTAG 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.