RLG00000017119

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001208
Physical Location & Seq
Reverse (-)
12979489 .. 12980532
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017119

Sequence Viewer

Length: 720 bp
ATGGCTGAATCAATCGATGCCGTCTCCGTTGACATGGAGAAGATCTATCTCGGCGGAAAGGAACATTTTATTCGAACTGGCTGTGGTTTTGTGTCCGTTATAGTCTGTGGGGACCGAGATAAGCCTGCATTTATCACAGCACCATCTTGGACCGAATGGTTTTATAATAAGGTGATATCAAATATGCTATATTTCTATGGGATGTGTGGTTTGCTAAAGGAGCGTTTGCTTCAGCGTTACTTTAGTAAGGAGGTTCGTGGTAGTGCTGAAGTTCCGGAGTCGGATATAGTTAAAGCATGCAGAAAGTTGTTGGAAGAGCGGCATAGCTCAAATGTTTTGAGATTTCTTAAAGCAATTAATCGGAGACCTGACATAACTGAAGGCTTGAAGTCACTAAGGTGTCGCACTCTCATTTTTGTTGGGGATAGCTCTCCTTTCCACCGTGAGGCTCTCCACATGATCTCAAAGTTGGATAGAAGAAATAGTGCCTTAGTAGAGGTCCAGGCTTGTGGATCGATGGTGACAGAGGAGCAGCCACATGCAATGTTGATACCCATGGAGTACTTCTTCATGGGGTATGGTTTTTACAGACCGTGTCATTTCAGAGAGAGCCCTAGGAGCCCGCTCATCCCATCTTGCATCTCCCCGGAGCTTCTCTCCCCTGAAAGCATGGGTCTGAAGCTAAAACCAATCAAGACCCATGTGTCGCTCGGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

240

Amino Acids

27.25

Weight (kDa)

8.75

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 46 - 197 1.4e-49 Ndr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 165, 718
AasI GACNNNNNNGTC 1 cut(s) 703
AccBSI CCGCTC 2 cut(s) 319, 625
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 3 cut(s) 54, 319, 623
AclWI GGATC 1 cut(s) 520
AcuI CTGAAG 4 cut(s) 215, 288, 399, 698
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 1 cut(s) 445
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 501
AleI CACNNNNGTG 1 cut(s) 397
AluBI AGCT 4 cut(s) 327, 429, 652, 682
AluI AGCT 4 cut(s) 327, 429, 652, 682
Alw26I GTCTC 2 cut(s) 28, 358
AlwI GGATC 1 cut(s) 520
Aor13HI TCCGGA 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 532
AseI ATTAAT 1 cut(s) 357
AspA2I CCTAGG 1 cut(s) 614
AspS9I GGNCC 3 cut(s) 112, 150, 499
AsuC2I CCSGG 1 cut(s) 647
AsuHPI GGTGA 2 cut(s) 184, 532
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 3 cut(s) 112, 150, 499
AvrII CCTAGG 1 cut(s) 614
BanII GRGCYC 2 cut(s) 614, 623
BbvI GCAGC 1 cut(s) 544
BccI CCATC 3 cut(s) 151, 511, 640
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 1 cut(s) 503
BcnI CCSGG 1 cut(s) 647
BcoDI GTCTC 2 cut(s) 28, 358
BfaI CTAG 1 cut(s) 615
BglII AGATCT 1 cut(s) 42
BisI GCNGC 2 cut(s) 320, 533
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 2 cut(s) 321, 534
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 2 cut(s) 503, 647
Bme18I GGWCC 3 cut(s) 112, 150, 499
BmgT120I GGNCC 3 cut(s) 112, 150, 499
BmiI GGNNCC 2 cut(s) 113, 620
BmrFI CCNGG 2 cut(s) 503, 647
BmsI GCATC 2 cut(s) 7, 648
BplI GAGNNNNNCTC 2 cut(s) 641, 673
Bpu14I TTCGAA 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 647
Bsa29I ATCGAT 2 cut(s) 15, 515
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 555, 614, 645
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 445
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 274
BseBI CCWGG 1 cut(s) 503
BseCI ATCGAT 2 cut(s) 15, 515
BseDI CCNNGG 3 cut(s) 555, 614, 645
BseGI GGATG 2 cut(s) 207, 627
BseLI CCNNNNNNNGG 1 cut(s) 445
BseMI GCAATG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 1 cut(s) 544
BshVI ATCGAT 2 cut(s) 15, 515
BsiSI CCGG 2 cut(s) 275, 647
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 445
BsmAI GTCTC 2 cut(s) 28, 358
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 358
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 614, 623
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 3 cut(s) 42, 459, 512
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 3 cut(s) 54, 319, 623
BspDI ATCGAT 2 cut(s) 15, 515
BspEI TCCGGA 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 113, 620
BspPI GGATC 1 cut(s) 520
BspQI GCTCTTC 1 cut(s) 309
BspT104I TTCGAA 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 358
BsrBI CCGCTC 2 cut(s) 319, 625
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 3 cut(s) 555, 614, 645
BssMI GATC 3 cut(s) 42, 459, 512
BssT1I CCWWGG 2 cut(s) 555, 614
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 443, 594
Bst6I CTCTTC 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 3 cut(s) 126, 298, 623
BstDEI CTNAG 2 cut(s) 395, 490
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 207, 627
BstKTI GATC 3 cut(s) 45, 462, 515
BstMAI GTCTC 2 cut(s) 28, 358
BstMBI GATC 3 cut(s) 42, 459, 512
BstMWI GCNNNNNNNGC 2 cut(s) 220, 618
BstNI CCWGG 1 cut(s) 503
BstNSI RCATGY 2 cut(s) 300, 542
BstSCI CCNGG 2 cut(s) 501, 645
BstV1I GCAGC 1 cut(s) 544
BstX2I RGATCY 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 1 cut(s) 42
Bsu15I ATCGAT 2 cut(s) 15, 515
BsuTUI ATCGAT 2 cut(s) 15, 515
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 2 cut(s) 207, 627
Cac8I GCNNGC 3 cut(s) 126, 298, 623
Cfr13I GGNCC 3 cut(s) 112, 150, 499
ClaI ATCGAT 2 cut(s) 15, 515
Csp6I GTAC 1 cut(s) 562
CviAII CATG 8 cut(s) 34, 297, 457, 539, 556, 571, 670, 701
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 2 cut(s) 395, 490
DpnI GATC 3 cut(s) 44, 461, 514
DpnII GATC 3 cut(s) 42, 459, 512
DrdI GACNNNNNNGTC 1 cut(s) 703
DseDI GACNNNNNNGTC 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 1 cut(s) 69
Eco130I CCWWGG 2 cut(s) 555, 614
Eco24I GRGCYC 2 cut(s) 614, 623
Eco31I GGTCTC 1 cut(s) 358
Eco32I GATATC 1 cut(s) 177
Eco47I GGWCC 3 cut(s) 112, 150, 499
Eco57I CTGAAG 4 cut(s) 215, 288, 399, 698
EcoRII CCWGG 1 cut(s) 501
EcoRV GATATC 1 cut(s) 177
EcoT14I CCWWGG 2 cut(s) 555, 614
EcoT38I GRGCYC 2 cut(s) 614, 623
ErhI CCWWGG 2 cut(s) 555, 614
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 8 cut(s) 37, 300, 460, 542, 559, 574, 673, 704
FaqI GGGAC 1 cut(s) 125
FatI CATG 8 cut(s) 33, 296, 456, 538, 555, 570, 669, 700
FauI CCCGC 1 cut(s) 630
Fnu4HI GCNGC 2 cut(s) 320, 533
FokI GGATG 2 cut(s) 214, 614
FriOI GRGCYC 2 cut(s) 614, 623
Fsp4HI GCNGC 2 cut(s) 320, 533
FspBI CTAG 1 cut(s) 615
GluI GCNGC 2 cut(s) 320, 533
HapII CCGG 2 cut(s) 275, 647
Hin1II CATG 8 cut(s) 37, 300, 460, 542, 559, 574, 673, 704
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 2 cut(s) 8, 278
HpaII CCGG 2 cut(s) 275, 647
HphI GGTGA 2 cut(s) 184, 532
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 5 cut(s) 283, 363, 605, 678, 713
Hpy188III TCNNGA 2 cut(s) 275, 694
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 374
HpyCH4III ACNGT 2 cut(s) 443, 594
HpyCH4V TGCA 4 cut(s) 128, 300, 542, 639
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 618
HpyF3I CTNAG 2 cut(s) 395, 490
Hsp92II CATG 8 cut(s) 37, 300, 460, 542, 559, 574, 673, 704
Kpn2I TCCGGA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 42, 459, 512
LguI GCTCTTC 1 cut(s) 309
LmnI GCTCC 4 cut(s) 220, 529, 618, 649
LpnPI CCDG 8 cut(s) 63, 138, 288, 381, 488, 515, 660, 675
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 2 cut(s) 7, 648
MaeI CTAG 1 cut(s) 615
MaeIII GTNAC 3 cut(s) 236, 390, 520
MalI GATC 3 cut(s) 44, 461, 514
MbiI CCGCTC 2 cut(s) 319, 625
MboI GATC 3 cut(s) 42, 459, 512
MboII GAAGA 4 cut(s) 52, 326, 489, 559
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 2 cut(s) 614, 623
MluCI AATT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 3 cut(s) 261, 291, 450
MnlI CCTC 4 cut(s) 244, 439, 490, 520
MroI TCCGGA 1 cut(s) 274
MseI TTAA 3 cut(s) 291, 348, 357
MslI CAYNNNNRTG 1 cut(s) 397
MspI CCGG 2 cut(s) 275, 647
MspR9I CCNGG 2 cut(s) 503, 647
MvaI CCWGG 1 cut(s) 503
MwoI GCNNNNNNNGC 2 cut(s) 220, 618
NciI CCSGG 1 cut(s) 647
NcoI CCATGG 1 cut(s) 555
NdeII GATC 3 cut(s) 42, 459, 512
NlaIII CATG 8 cut(s) 37, 300, 460, 542, 559, 574, 673, 704
NlaIV GGNNCC 2 cut(s) 113, 620
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 390, 520
NspI RCATGY 2 cut(s) 300, 542
NspV TTCGAA 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 397
PaeI GCATGC 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 594
PkrI GCNGC 2 cut(s) 321, 534
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PshBI ATTAAT 1 cut(s) 357
PsiI TTATAA 2 cut(s) 165, 718
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 2 cut(s) 113, 620
PspPI GGNCC 3 cut(s) 112, 150, 499
PsuI RGATCY 1 cut(s) 42
PsyI GACNNNGTC 1 cut(s) 594
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 397
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 3 cut(s) 291, 348, 357
SatI GCNGC 2 cut(s) 320, 533
Sau3AI GATC 3 cut(s) 42, 459, 512
Sau96I GGNCC 3 cut(s) 112, 150, 499
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 503, 647
SduI GDGCHC 2 cut(s) 614, 623
SetI ASST 9 cut(s) 174, 255, 329, 370, 401, 431, 501, 654, 684
SfaNI GCATC 2 cut(s) 7, 648
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 3 cut(s) 112, 150, 499
SmiMI CAYNNNNRTG 1 cut(s) 397
SphI GCATGC 1 cut(s) 300
Sse9I AATT 1 cut(s) 354
SsiI CCGC 3 cut(s) 54, 319, 623
SspMI CTAG 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 501, 645
StyI CCWWGG 2 cut(s) 555, 614
TaaI ACNGT 2 cut(s) 443, 594
TaqI TCGA 3 cut(s) 15, 73, 515
TaqII GACCGA 2 cut(s) 129, 167
TasI AATT 1 cut(s) 354
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 291, 348, 357
Tru9I TTAA 3 cut(s) 291, 348, 357
TseFI GTSAC 2 cut(s) 390, 520
TseI GCWGC 1 cut(s) 532
Tsp45I GTSAC 2 cut(s) 390, 520
TspDTI ATGAA 1 cut(s) 559
TspGWI ACGGA 2 cut(s) 16, 85
Tth111I GACNNNGTC 1 cut(s) 594
VpaK11BI GGWCC 3 cut(s) 112, 150, 499
VspI ATTAAT 1 cut(s) 357
XceI RCATGY 2 cut(s) 300, 542
XmaJI CCTAGG 1 cut(s) 614
XspI CTAG 1 cut(s) 615
ZrmI AGTACT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.