Rh2DG157000

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001208
Physical Location & Seq
Reverse (-)
13119888 .. 13120931
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG157000.1

Sequence Viewer

Length: 1044 bp
ATGGCTGAATCAATAGACGCCGTCTCCGTTGACATGGAGAAGATCTATCTCGGCGGAAAGGAACATTTTATTCGAACTGGCTGTGGTTCTGTGTCCGTTATAGTCTGTGGGGACCAAGATAAGCCTGCACTTATCACGTATCCTGATTTAGCTCTAAATCATATGTCTTGTTTCCAAGGGTTATTCTCTTGTCCTGAAGCAGCTTCTTTCCTGCTCCACAACTTCTGCATATACCATATTAGTCCTCCTGGTCATGAGTTAGGAGCTGCTTCGATTAGTCTTGATGATCCTGTACCTTCTGCTGACGACTTGGCAGATCAGATCCTTGAAGTTCTAAACTTTTTCGGGCTTGGTGCAGTTATGTGCATGGGAGTGACAGCGGGTGCTTATATCCTTTCCTTATTTGCTACGAAATATAGGAAACGCGTTCTTGGTTTGATACTTGTATCTCCATTATGCAGAGCACCATCTTGGACCGAATGGTTTTATAATAAGGTGATATCAAATATGCTATATTTCTATGGGATGTGTGGTTTGCTAAAGGAGCGTTTGCTTCAGCGTTACTTTAGTAAGGAGGTTCGTGGTAGTGCTGAAGTTCCGGAGTCGGATATAGTTCAAGCATGCAGAAAGTTGTTGGAAGAGAGGCAGAGCTCAAATGTTTGGAGATTTCTTAAAGCAATTAATCGGAGACCTGACATAACTGAAGGCTTGAAGTCACTAAGGTGTCGCACTCTCATTTTTGTTGGGGATAGCTCTCCTTTCCACCGTGAGGCTCTCCACATGATCTCAAAGTTGGATAGAAGAAATAGTGCCTTAGTAGAGGTCCAGGCTTGTGGATCGATGGTGACAGAGGAGCAGCCACATGCAATGTTGATACCCATGGAGTACTTGTTCATGGGGTATGGTTTTTACAGACCGTGTCATTTCAGAGAGAGCCCTAGGAGCCCACTCATCCCAACTTGCATCTCCCCGGAGCTTCTCTCCCAGGAAAGCATGGGTCTGAAGCTAAAACCAATCAAGACCCGTGTGTCGCTCGGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

347

Amino Acids

38.86

Weight (kDa)

7.08

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 305 3.9e-113 Ndr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 1042
AasI GACNNNNNNGTC 1 cut(s) 1027
AccII CGCG 1 cut(s) 426
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 2 cut(s) 54, 380
AclWI GGATC 3 cut(s) 281, 316, 844
AcuI CTGAAG 5 cut(s) 216, 539, 612, 723, 1022
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 2 cut(s) 294, 887
AfiI CCNNNNNNNGG 1 cut(s) 769
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 3 cut(s) 329, 617, 712
AjnI CCWGG 3 cut(s) 247, 825, 984
AleI CACNNNNGTG 1 cut(s) 721
AloI GAACNNNNNNTCC 2 cut(s) 875, 907
AluBI AGCT 7 cut(s) 152, 203, 266, 651, 753, 976, 1006
AluI AGCT 7 cut(s) 152, 203, 266, 651, 753, 976, 1006
Alw21I GWGCWC 2 cut(s) 466, 653
Alw26I GTCTC 2 cut(s) 28, 682
AlwI GGATC 3 cut(s) 281, 316, 844
Aor13HI TCCGGA 1 cut(s) 598
ApeKI GCWGC 3 cut(s) 200, 266, 856
AseI ATTAAT 1 cut(s) 681
AspA2I CCTAGG 1 cut(s) 938
AspS9I GGNCC 3 cut(s) 112, 474, 823
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 2 cut(s) 508, 856
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 3 cut(s) 112, 474, 823
AvrII CCTAGG 1 cut(s) 938
BanII GRGCYC 3 cut(s) 653, 938, 947
Bbv12I GWGCWC 2 cut(s) 466, 653
BbvI GCAGC 3 cut(s) 212, 253, 868
BccI CCATC 2 cut(s) 475, 835
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 3 cut(s) 249, 827, 986
BciVI GTATCC 1 cut(s) 150
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 2 cut(s) 28, 682
BfaI CTAG 1 cut(s) 939
BfuI GTATCC 1 cut(s) 150
BglII AGATCT 1 cut(s) 42
BisI GCNGC 3 cut(s) 201, 267, 857
BlnI CCTAGG 1 cut(s) 938
BlsI GCNGC 3 cut(s) 202, 268, 858
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 4 cut(s) 249, 827, 971, 986
Bme18I GGWCC 3 cut(s) 112, 474, 823
BmgT120I GGNCC 3 cut(s) 112, 474, 823
BmiI GGNNCC 2 cut(s) 113, 944
BmrFI CCNGG 4 cut(s) 249, 827, 971, 986
BmsI GCATC 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 965, 997
Bpu14I TTCGAA 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 971
Bsa29I ATCGAT 1 cut(s) 839
BsaAI YACGTR 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 5 cut(s) 175, 879, 938, 969, 984
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 875, 905
Bsc4I CCNNNNNNNGG 1 cut(s) 769
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 873
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 3 cut(s) 249, 827, 986
BseCI ATCGAT 1 cut(s) 839
BseDI CCNNGG 5 cut(s) 175, 879, 938, 969, 984
BseGI GGATG 2 cut(s) 531, 951
BseLI CCNNNNNNNGG 1 cut(s) 769
BseMI GCAATG 1 cut(s) 873
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 212, 253, 868
BsgI GTGCAG 2 cut(s) 111, 375
Bsh1236I CGCG 1 cut(s) 426
BshVI ATCGAT 1 cut(s) 839
BsiHKAI GWGCWC 2 cut(s) 466, 653
BsiSI CCGG 2 cut(s) 599, 971
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 769
BsmAI GTCTC 2 cut(s) 28, 682
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 682
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 4 cut(s) 466, 653, 938, 947
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 6 cut(s) 42, 286, 316, 321, 783, 836
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 2 cut(s) 54, 380
BspDI ATCGAT 1 cut(s) 839
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 1 cut(s) 426
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 113, 944
BspPI GGATC 3 cut(s) 281, 316, 844
BspT104I TTCGAA 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 873
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 5 cut(s) 175, 879, 938, 969, 984
BssMI GATC 6 cut(s) 42, 286, 316, 321, 783, 836
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 175, 879, 938
Bst2UI CCWGG 3 cut(s) 249, 827, 986
Bst4CI ACNGT 2 cut(s) 767, 918
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 18
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 126, 622
BstDEI CTNAG 2 cut(s) 719, 814
BstDSI CCRYGG 1 cut(s) 879
BstF5I GGATG 2 cut(s) 531, 951
BstFNI CGCG 1 cut(s) 426
BstKTI GATC 6 cut(s) 45, 289, 319, 324, 786, 839
BstMAI GTCTC 2 cut(s) 28, 682
BstMBI GATC 6 cut(s) 42, 286, 316, 321, 783, 836
BstMWI GCNNNNNNNGC 2 cut(s) 544, 942
BstNI CCWGG 3 cut(s) 249, 827, 986
BstNSI RCATGY 2 cut(s) 624, 866
BstSCI CCNGG 4 cut(s) 247, 825, 969, 984
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 3 cut(s) 212, 253, 868
BstX2I RGATCY 2 cut(s) 42, 321
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 2 cut(s) 42, 321
Bsu15I ATCGAT 1 cut(s) 839
BsuI GTATCC 1 cut(s) 150
BsuTUI ATCGAT 1 cut(s) 839
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 2 cut(s) 531, 951
Cac8I GCNNGC 2 cut(s) 126, 622
CciI TCATGA 1 cut(s) 253
Cfr13I GGNCC 3 cut(s) 112, 474, 823
ClaI ATCGAT 1 cut(s) 839
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 293, 886
CviAII CATG 9 cut(s) 34, 254, 367, 621, 781, 863, 880, 895, 994
CviQI GTAC 2 cut(s) 293, 886
DdeI CTNAG 2 cut(s) 719, 814
DpnI GATC 6 cut(s) 44, 288, 318, 323, 785, 838
DpnII GATC 6 cut(s) 42, 286, 316, 321, 783, 836
DrdI GACNNNNNNGTC 1 cut(s) 1027
DseDI GACNNNNNNGTC 1 cut(s) 1027
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 1 cut(s) 651
Eco130I CCWWGG 3 cut(s) 175, 879, 938
Eco24I GRGCYC 3 cut(s) 653, 938, 947
Eco31I GGTCTC 1 cut(s) 682
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 3 cut(s) 112, 474, 823
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 5 cut(s) 216, 539, 612, 723, 1022
EcoICRI GAGCTC 1 cut(s) 651
EcoRII CCWGG 3 cut(s) 247, 825, 984
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 3 cut(s) 175, 879, 938
EcoT38I GRGCYC 3 cut(s) 653, 938, 947
ErhI CCWWGG 3 cut(s) 175, 879, 938
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 9 cut(s) 37, 257, 370, 624, 784, 866, 883, 898, 997
FaqI GGGAC 1 cut(s) 125
FatI CATG 9 cut(s) 33, 253, 366, 620, 780, 862, 879, 894, 993
FauI CCCGC 1 cut(s) 373
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 201, 267, 857
FokI GGATG 2 cut(s) 538, 938
FriOI GRGCYC 3 cut(s) 653, 938, 947
Fsp4HI GCNGC 3 cut(s) 201, 267, 857
FspBI CTAG 1 cut(s) 939
GluI GCNGC 3 cut(s) 201, 267, 857
HapII CCGG 2 cut(s) 599, 971
HgaI GACGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 9 cut(s) 37, 257, 370, 624, 784, 866, 883, 898, 997
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 2 cut(s) 8, 602
HpaII CCGG 2 cut(s) 599, 971
HphI GGTGA 2 cut(s) 508, 856
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 6 cut(s) 321, 607, 687, 929, 1002, 1037
Hpy188III TCNNGA 6 cut(s) 143, 194, 254, 281, 599, 1018
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 306, 698
HpyCH4III ACNGT 2 cut(s) 767, 918
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 8 cut(s) 128, 228, 356, 366, 459, 624, 866, 963
HpyF10VI GCNNNNNNNGC 2 cut(s) 544, 942
HpyF3I CTNAG 2 cut(s) 719, 814
HpySE526I ACGT 1 cut(s) 137
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 9 cut(s) 37, 257, 370, 624, 784, 866, 883, 898, 997
Kpn2I TCCGGA 1 cut(s) 598
Kzo9I GATC 6 cut(s) 42, 286, 316, 321, 783, 836
LmnI GCTCC 6 cut(s) 219, 263, 544, 853, 942, 973
Lsp1109I GCAGC 3 cut(s) 212, 253, 868
LweI GCATC 1 cut(s) 972
MaeI CTAG 1 cut(s) 939
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 4 cut(s) 373, 560, 714, 844
MalI GATC 6 cut(s) 44, 288, 318, 323, 785, 838
MboI GATC 6 cut(s) 42, 286, 316, 321, 783, 836
MboII GAAGA 3 cut(s) 52, 650, 813
MflI RGATCY 2 cut(s) 42, 321
MhlI GDGCHC 4 cut(s) 466, 653, 938, 947
MluCI AATT 1 cut(s) 678
MluI ACGCGT 1 cut(s) 424
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 3 cut(s) 585, 615, 774
MnlI CCTC 6 cut(s) 255, 568, 636, 763, 814, 844
MroI TCCGGA 1 cut(s) 598
MseI TTAA 2 cut(s) 672, 681
MslI CAYNNNNRTG 2 cut(s) 371, 721
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 2 cut(s) 599, 971
MspR9I CCNGG 4 cut(s) 249, 827, 971, 986
MvaI CCWGG 3 cut(s) 249, 827, 986
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 2 cut(s) 544, 942
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 879
NdeI CATATG 1 cut(s) 162
NdeII GATC 6 cut(s) 42, 286, 316, 321, 783, 836
NlaIII CATG 9 cut(s) 37, 257, 370, 624, 784, 866, 883, 898, 997
NlaIV GGNNCC 2 cut(s) 113, 944
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 373, 714, 844
NspI RCATGY 2 cut(s) 624, 866
NspV TTCGAA 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 721
PaeI GCATGC 1 cut(s) 624
PagI TCATGA 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 2 cut(s) 20, 918
PkrI GCNGC 3 cut(s) 202, 268, 858
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
Ppu21I YACGTR 1 cut(s) 138
PshBI ATTAAT 1 cut(s) 681
PsiI TTATAA 2 cut(s) 489, 1042
Psp124BI GAGCTC 1 cut(s) 653
Psp6I CCWGG 3 cut(s) 247, 825, 984
PspGI CCWGG 3 cut(s) 247, 825, 984
PspN4I GGNNCC 2 cut(s) 113, 944
PspPI GGNCC 3 cut(s) 112, 474, 823
PsuI RGATCY 2 cut(s) 42, 321
PsyI GACNNNGTC 2 cut(s) 20, 918
RsaI GTAC 2 cut(s) 294, 887
RsaNI GTAC 2 cut(s) 293, 886
RseI CAYNNNNRTG 2 cut(s) 371, 721
SacI GAGCTC 1 cut(s) 653
SaqAI TTAA 2 cut(s) 672, 681
SatI GCNGC 3 cut(s) 201, 267, 857
Sau3AI GATC 6 cut(s) 42, 286, 316, 321, 783, 836
Sau96I GGNCC 3 cut(s) 112, 474, 823
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 4 cut(s) 249, 827, 971, 986
SduI GDGCHC 4 cut(s) 466, 653, 938, 947
SfaNI GCATC 1 cut(s) 972
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 3 cut(s) 112, 474, 823
SmiMI CAYNNNNRTG 2 cut(s) 371, 721
SphI GCATGC 1 cut(s) 624
Sse9I AATT 1 cut(s) 678
SsiI CCGC 2 cut(s) 54, 380
SspMI CTAG 1 cut(s) 939
SstI GAGCTC 1 cut(s) 653
StyD4I CCNGG 4 cut(s) 247, 825, 969, 984
StyI CCWWGG 3 cut(s) 175, 879, 938
TaaI ACNGT 2 cut(s) 767, 918
TaiI ACGT 1 cut(s) 140
TaqI TCGA 3 cut(s) 73, 272, 839
TaqII GACCGA 1 cut(s) 491
TasI AATT 1 cut(s) 678
TatI WGTACW 1 cut(s) 885
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 672, 681
Tru9I TTAA 2 cut(s) 672, 681
TseFI GTSAC 3 cut(s) 373, 714, 844
TseI GCWGC 3 cut(s) 200, 266, 856
Tsp45I GTSAC 3 cut(s) 373, 714, 844
TspDTI ATGAA 1 cut(s) 883
TspGWI ACGGA 2 cut(s) 16, 85
Tth111I GACNNNGTC 2 cut(s) 20, 918
VpaK11BI GGWCC 3 cut(s) 112, 474, 823
VspI ATTAAT 1 cut(s) 681
XceI RCATGY 2 cut(s) 624, 866
XmaJI CCTAGG 1 cut(s) 938
XspI CTAG 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.