pycom04g19570

Ndr family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001208
Physical Location & Seq
Forward (+)
21279850 .. 21280766
917 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19570.4

Sequence Viewer

Length: 540 bp
ATGTGTGATATAGTAAAGGAATGCCTTCTGCAGCGTTACTTCAGTAAGGAGCTTAGGTTTGGCATACACGGTGGAGAGTCAGATGTTATAGAAGCTTGCCAAAGGTTATTGGATGAAAGACAAAGTTCGAATGTCTTGCGGTTCCTTCAAGCAACTAATGAGAGGCACGACCTTACCCAGGGCTTGAAGAATTTGCAGTGTAAGACTCTTATTTTTGTGGGTGAAAGAAGTCCATTCCATGCTGAATCCGTCTATATGAATGCCAAAATGAACCGAAGAAGTTGTGCTCTTGTTGAAGTTCAGGCATGTGGCTCGCTGGTAACAGAAGAGCACCCATACGCAATGATAACTCCGATTGAACTCTTCCTCAGGGGTTTTGGGTACTACAGACAATTACATTTTACTTCCTCATCAAGTAACGATTCGAATCCCACCAGCCCCTCAAAAATCAATTCATGCATAGCGCCCGAACTTCTTTCTCCTGAGAGTCTGGGAATCAAGCTCAAACCGATCAAAACACGGGTAGCCATCGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

180

Amino Acids

20.23

Weight (kDa)

7.6

Isoelectric Point (pI)

71.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 139
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 25
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 4 cut(s) 149, 187, 296, 359
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 3 cut(s) 52, 95, 502
AluI AGCT 3 cut(s) 52, 95, 502
Alw21I GWGCWC 2 cut(s) 289, 333
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 190
Asp700I GAANNNNTTC 1 cut(s) 24
AspLEI GCGC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 233
AsuII TTCGAA 2 cut(s) 128, 425
AxyI CCTNAGG 1 cut(s) 368
Bbv12I GWGCWC 2 cut(s) 289, 333
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 536
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 294, 328
BciT130I CCWGG 1 cut(s) 179
BfmI CTRYAG 2 cut(s) 29, 385
BfoI RGCGCY 1 cut(s) 467
BisI GCNGC 1 cut(s) 32
BlsI GCNGC 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 179
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 179
Bpu10I CCTNAGC 1 cut(s) 53
Bpu14I TTCGAA 2 cut(s) 128, 425
BsaBI GATNNNNATC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 177, 178
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse21I CCTNAGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 426
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 177, 178
BseGI GGATG 1 cut(s) 118
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 382, 474
BseXI GCAGC 1 cut(s) 43
BsiHKAI GWGCWC 2 cut(s) 289, 333
BslI CCNNNNNNNGG 1 cut(s) 178
BsmI GAATGC 2 cut(s) 26, 265
Bsp119I TTCGAA 2 cut(s) 128, 425
Bsp1286I GDGCHC 2 cut(s) 289, 333
Bsp143I GATC 1 cut(s) 510
BspACI CCGC 1 cut(s) 139
BspCNI CTCAG 2 cut(s) 381, 475
BspLI GGNNCC 1 cut(s) 143
BspMAI CTGCAG 1 cut(s) 33
BspQI GCTCTTC 1 cut(s) 321
BspT104I TTCGAA 2 cut(s) 128, 425
BsrDI GCAATG 1 cut(s) 348
BssECI CCNNGG 2 cut(s) 177, 178
BssMI GATC 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 2 cut(s) 321, 368
BstBI TTCGAA 2 cut(s) 128, 425
BstC8I GCNNGC 2 cut(s) 97, 314
BstDEI CTNAG 3 cut(s) 53, 368, 483
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 1 cut(s) 513
BstMBI GATC 1 cut(s) 510
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 2 cut(s) 29, 385
BstV1I GCAGC 1 cut(s) 43
Bsu36I CCTNAGG 1 cut(s) 368
BtsCI GGATG 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 203
BtsIMutI CAGTG 1 cut(s) 203
Cac8I GCNNGC 2 cut(s) 97, 314
CfoI GCGC 1 cut(s) 466
Csp6I GTAC 1 cut(s) 382
CviAII CATG 3 cut(s) 239, 306, 456
CviJI RGCY 7 cut(s) 52, 95, 183, 312, 438, 502, 527
CviKI_1 RGCY 7 cut(s) 52, 95, 183, 312, 438, 502, 527
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 3 cut(s) 53, 368, 483
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 321, 368
EarI CTCTTC 2 cut(s) 321, 368
Eco57I CTGAAG 1 cut(s) 25
Eco81I CCTNAGG 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 461
FaeI CATG 3 cut(s) 242, 309, 459
FatI CATG 3 cut(s) 238, 305, 455
Fnu4HI GCNGC 1 cut(s) 32
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 32
GlaI GCGC 1 cut(s) 465
GluI GCNGC 1 cut(s) 32
HaeII RGCGCY 1 cut(s) 467
HhaI GCGC 1 cut(s) 466
Hin1II CATG 3 cut(s) 242, 309, 459
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 7 cut(s) 77, 205, 245, 422, 427, 487, 495
HphI GGTGA 1 cut(s) 233
Hpy188I TCNGA 2 cut(s) 82, 354
Hpy188III TCNNGA 1 cut(s) 482
HpyAV CCTTC 2 cut(s) 35, 155
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 3 cut(s) 31, 196, 459
HpyF3I CTNAG 3 cut(s) 53, 368, 483
Hsp92II CATG 3 cut(s) 242, 309, 459
HspAI GCGC 1 cut(s) 464
Kzo9I GATC 1 cut(s) 510
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 8 cut(s) 164, 191, 287, 302, 355, 448, 476, 495
Lsp1109I GCAGC 1 cut(s) 43
MaeIII GTNAC 3 cut(s) 35, 319, 416
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 4 cut(s) 199, 288, 338, 355
MhlI GDGCHC 2 cut(s) 289, 333
MluCI AATT 3 cut(s) 190, 392, 451
MlyI GAGTC 3 cut(s) 86, 199, 496
MnlI CCTC 4 cut(s) 156, 377, 418, 451
Mph1103I ATGCAT 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 179
Mva1269I GAATGC 2 cut(s) 26, 265
MvaI CCWGG 1 cut(s) 179
NdeII GATC 1 cut(s) 510
NlaIII CATG 3 cut(s) 242, 309, 459
NlaIV GGNNCC 1 cut(s) 143
NsiI ATGCAT 1 cut(s) 461
NspI RCATGY 1 cut(s) 309
NspV TTCGAA 2 cut(s) 128, 425
PasI CCCWGGG 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 321
PctI GAATGC 2 cut(s) 26, 265
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 4 cut(s) 245, 422, 427, 495
PkrI GCNGC 1 cut(s) 33
PleI GAGTC 3 cut(s) 85, 199, 495
PpsI GAGTC 3 cut(s) 85, 199, 495
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 33
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 321
SatI GCNGC 1 cut(s) 32
Sau3AI GATC 1 cut(s) 510
SchI GAGTC 3 cut(s) 86, 199, 496
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 2 cut(s) 289, 333
SetI ASST 6 cut(s) 54, 59, 97, 107, 174, 504
SfcI CTRYAG 2 cut(s) 29, 385
SfuI TTCGAA 2 cut(s) 128, 425
Sse9I AATT 3 cut(s) 190, 392, 451
SsiI CCGC 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 3 cut(s) 128, 425, 531
TasI AATT 3 cut(s) 190, 392, 451
TfiI GAWTC 4 cut(s) 245, 422, 427, 495
TscAI CASTG 1 cut(s) 203
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 129, 272, 284, 444
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 1 cut(s) 190
XceI RCATGY 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.