Rw2G006930

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001208
Physical Location & Seq
Forward (+)
6675075 .. 6679213
4139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006930.1

Sequence Viewer

Length: 1143 bp
ATGGCTGAATCAATCGACTCCGTCTCCCTTGATATGGAGAAGATCTATCTGGGTGGAAAGGAACATTTTATTCGAACTGGCTGTGGTTCTGTGTCTGTTATAGTTTATGGAGACCAAGATAAGCCGGCACTTATCACATATCCTGATTTAGCTCTAAATCATATGTCTTGTTTCCAAGGGCTATTCTTTTGTCCTGAAGCAGCTTCTTTGCTGCTCCACAACTTCTGCATATACCATATTAGTCCTCCTGGTCATGAGTTAGGAGCTGCTTCGATTAGTCTTGATGATCCTGTGCCTTCTGCTGATGACTTGGCAGATCAGATCCTTGAGGTTCTAAACTTTTTCGGGCTTGGTGCAGTTATGTGCATGGGTGTGACGGCGGGTGCTTATATCCTTTCCCTATTTGCTATGAAATATAGGGAACGCGTTCTTGGTTTGATACTTGTATCCCCATTATGCAGAGCACCATCTTGGACTGAATGGTTTTATAATAAGGTACTTCTTCCTAACCTAGCTGTTATTATTATTCTTTTTAGAAGTTGTGAAGCTCAATTGTTATACTATACTGTACTTATGATTACTTGTAATATGCAGGTGATATCAAACATGCTATATTTCTATGGCATGTGTGGTTTGTTAAAGGAGTGTTTACTTCAGCGTTACTTCAGTAAGGAGGTTCGTGGTAGTGCTGAAGTTCCAGAGTCGGATATAGTTCAAGCATGCAGAAAATTATTGGAAGAGAGGCAGAGCTCAAATGTTTGGAGATTTCTTCAAGCAATTAATCGGAGACCTGACATAACTGAAGGATTGAAGTCACTAAGGTGTCGCACTCTCATTTTTGTTGGGGATAGCTCTCCTTTCCACTCTGAGGCTCTCCACATGACCTCAAAGTTGGATAGAAGATATAGTGCCTTAGTAGAGGTCCAGGCTTGTGGATCGATGGTGACAGAGGAGCAGCCACATGCAATGTTGATACCCATGGAGTACTTCTTCATGGGGTATGGTTTGTACAGACCGTGTCATTTCAGCGAGAGCCCAAGGAGCCCACTCAGCCCATCTTGCATCTCCCCTGAGCTTCTCTCCCCGGAAAGCATGGGTCTGAAGCTAAAACCAATCAAGACCCGTGTTTCGCTCGGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

380

Amino Acids

42.57

Weight (kDa)

5.88

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 167 1.9e-64 Ndr family
Ndr PF03096 195 - 338 1.7e-44 Ndr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 1141
AarI CACCTGC 1 cut(s) 583
Acc36I ACCTGC 1 cut(s) 583
AccII CGCG 1 cut(s) 426
AciI CCGC 1 cut(s) 380
AclWI GGATC 3 cut(s) 281, 316, 943
AcuI CTGAAG 6 cut(s) 216, 638, 649, 711, 822, 1121
AfaI GTAC 4 cut(s) 498, 570, 986, 1010
AfiI CCNNNNNNNGG 2 cut(s) 34, 868
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 3 cut(s) 716, 773, 811
AjnI CCWGG 2 cut(s) 247, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AleI CACNNNNGTG 1 cut(s) 820
AluBI AGCT 9 cut(s) 152, 203, 266, 515, 548, 750, 852, 1075, 1105
AluI AGCT 9 cut(s) 152, 203, 266, 515, 548, 750, 852, 1075, 1105
Alw21I GWGCWC 2 cut(s) 466, 752
Alw26I GTCTC 3 cut(s) 28, 105, 781
AlwI GGATC 3 cut(s) 281, 316, 943
ApeKI GCWGC 4 cut(s) 200, 211, 266, 955
ArsI GACNNNNNNTTYG 2 cut(s) 1111, 1143
AseI ATTAAT 1 cut(s) 780
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 1 cut(s) 922
AsuC2I CCSGG 1 cut(s) 1085
AsuHPI GGTGA 2 cut(s) 607, 955
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 922
BanII GRGCYC 3 cut(s) 752, 1037, 1046
Bbv12I GWGCWC 2 cut(s) 466, 752
BbvI GCAGC 4 cut(s) 198, 212, 253, 967
BccI CCATC 3 cut(s) 475, 934, 1063
BceAI ACGGC 1 cut(s) 393
BciT130I CCWGG 2 cut(s) 249, 926
BciVI GTATCC 1 cut(s) 457
BcnI CCSGG 1 cut(s) 1085
BcoDI GTCTC 3 cut(s) 28, 105, 781
BfaI CTAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 583
BfuI GTATCC 1 cut(s) 457
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 201, 212, 267, 956
BlsI GCNGC 4 cut(s) 202, 213, 268, 957
BmcAI AGTACT 1 cut(s) 986
Bme1390I CCNGG 3 cut(s) 249, 926, 1085
Bme18I GGWCC 1 cut(s) 922
BmgT120I GGNCC 1 cut(s) 922
BmiI GGNNCC 1 cut(s) 1043
BmrFI CCNGG 3 cut(s) 249, 926, 1085
BmsI GCATC 1 cut(s) 1071
BplI GAGNNNNNCTC 2 cut(s) 1064, 1096
Bpu10I CCTNAGC 1 cut(s) 1071
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 1085
Bsa29I ATCGAT 1 cut(s) 938
BsaI GGTCTC 2 cut(s) 105, 781
BsaJI CCNNGG 4 cut(s) 175, 978, 1037, 1083
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 868
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 972
BseBI CCWGG 2 cut(s) 249, 926
BseCI ATCGAT 1 cut(s) 938
BseDI CCNNGG 4 cut(s) 175, 978, 1037, 1083
BseLI CCNNNNNNNGG 2 cut(s) 34, 868
BseMI GCAATG 1 cut(s) 972
BseMII CTCAG 3 cut(s) 858, 1062, 1063
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 965
BseXI GCAGC 4 cut(s) 198, 212, 253, 967
BsgI GTGCAG 1 cut(s) 375
Bsh1236I CGCG 1 cut(s) 426
BshVI ATCGAT 1 cut(s) 938
BsiHKAI GWGCWC 2 cut(s) 466, 752
BsiSI CCGG 2 cut(s) 125, 1085
BslI CCNNNNNNNGG 2 cut(s) 34, 868
BsmAI GTCTC 3 cut(s) 28, 105, 781
BsmBI CGTCTC 1 cut(s) 28
Bso31I GGTCTC 2 cut(s) 105, 781
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 4 cut(s) 466, 752, 1037, 1046
Bsp1407I TGTACA 1 cut(s) 1008
Bsp143I GATC 5 cut(s) 42, 286, 316, 321, 935
Bsp19I CCATGG 1 cut(s) 978
BspACI CCGC 1 cut(s) 380
BspCNI CTCAG 3 cut(s) 859, 1062, 1063
BspDI ATCGAT 1 cut(s) 938
BspFNI CGCG 1 cut(s) 426
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 1043
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 3 cut(s) 281, 316, 943
BspT104I TTCGAA 1 cut(s) 73
BspTNI GGTCTC 2 cut(s) 105, 781
BsrDI GCAATG 1 cut(s) 972
BsrFI RCCGGY 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 1008
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 4 cut(s) 175, 978, 1037, 1083
BssMI GATC 5 cut(s) 42, 286, 316, 321, 935
BssT1I CCWWGG 3 cut(s) 175, 978, 1037
Bst2UI CCWGG 2 cut(s) 249, 926
Bst4CI ACNGT 2 cut(s) 568, 1017
Bst6I CTCTTC 1 cut(s) 732
BstAUI TGTACA 1 cut(s) 1008
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 126, 721
BstDEI CTNAG 5 cut(s) 818, 867, 913, 1049, 1071
BstDSI CCRYGG 1 cut(s) 978
BstFNI CGCG 1 cut(s) 426
BstKTI GATC 5 cut(s) 45, 289, 319, 324, 938
BstMAI GTCTC 3 cut(s) 28, 105, 781
BstMBI GATC 5 cut(s) 42, 286, 316, 321, 935
BstMWI GCNNNNNNNGC 3 cut(s) 1041, 1050, 1059
BstNI CCWGG 2 cut(s) 249, 926
BstNSI RCATGY 4 cut(s) 610, 628, 723, 965
BstSCI CCNGG 3 cut(s) 247, 924, 1083
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 198, 212, 253, 967
BstX2I RGATCY 2 cut(s) 42, 321
BstXI CCANNNNNNTGG 1 cut(s) 932
BstYI RGATCY 2 cut(s) 42, 321
Bsu15I ATCGAT 1 cut(s) 938
BsuI GTATCC 1 cut(s) 457
BsuTUI ATCGAT 1 cut(s) 938
BtgI CCRYGG 1 cut(s) 978
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 2 cut(s) 126, 721
CciI TCATGA 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 922
ClaI ATCGAT 1 cut(s) 938
Csp6I GTAC 4 cut(s) 497, 569, 985, 1009
CviQI GTAC 4 cut(s) 497, 569, 985, 1009
DdeI CTNAG 5 cut(s) 818, 867, 913, 1049, 1071
DpnI GATC 5 cut(s) 44, 288, 318, 323, 937
DpnII GATC 5 cut(s) 42, 286, 316, 321, 935
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Ecl136II GAGCTC 1 cut(s) 750
Eco130I CCWWGG 3 cut(s) 175, 978, 1037
Eco24I GRGCYC 3 cut(s) 752, 1037, 1046
Eco31I GGTCTC 2 cut(s) 105, 781
Eco32I GATATC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 922
Eco53kI GAGCTC 1 cut(s) 750
Eco57I CTGAAG 6 cut(s) 216, 638, 649, 711, 822, 1121
EcoICRI GAGCTC 1 cut(s) 750
EcoRII CCWGG 2 cut(s) 247, 924
EcoRV GATATC 1 cut(s) 600
EcoT14I CCWWGG 3 cut(s) 175, 978, 1037
EcoT38I GRGCYC 3 cut(s) 752, 1037, 1046
ErhI CCWWGG 3 cut(s) 175, 978, 1037
Esp3I CGTCTC 1 cut(s) 28
FauI CCCGC 1 cut(s) 373
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 4 cut(s) 201, 212, 267, 956
FriOI GRGCYC 3 cut(s) 752, 1037, 1046
Fsp4HI GCNGC 4 cut(s) 201, 212, 267, 956
FspBI CTAG 1 cut(s) 512
GluI GCNGC 4 cut(s) 201, 212, 267, 956
HapII CCGG 2 cut(s) 125, 1085
HinfI GANTC 3 cut(s) 8, 17, 701
HpaII CCGG 2 cut(s) 125, 1085
HphI GGTGA 2 cut(s) 607, 955
Hpy166II GTNNAC 1 cut(s) 650
Hpy188I TCNGA 6 cut(s) 321, 706, 786, 868, 1101, 1136
Hpy188III TCNNGA 6 cut(s) 143, 194, 254, 281, 698, 1117
Hpy8I GTNNAC 1 cut(s) 650
HpyAV CCTTC 2 cut(s) 306, 797
HpyCH4III ACNGT 2 cut(s) 568, 1017
HpyCH4V TGCA 8 cut(s) 228, 356, 366, 459, 592, 723, 965, 1062
HpyF10VI GCNNNNNNNGC 3 cut(s) 1041, 1050, 1059
HpyF3I CTNAG 5 cut(s) 818, 867, 913, 1049, 1071
KroI GCCGGC 1 cut(s) 124
KroNI GCCGGC 1 cut(s) 126
Kzo9I GATC 5 cut(s) 42, 286, 316, 321, 935
LmnI GCTCC 4 cut(s) 219, 263, 952, 1041
Lsp1109I GCAGC 4 cut(s) 198, 212, 253, 967
LweI GCATC 1 cut(s) 1071
MaeI CTAG 1 cut(s) 512
MaeIII GTNAC 4 cut(s) 373, 659, 813, 943
MalI GATC 5 cut(s) 44, 288, 318, 323, 937
MboI GATC 5 cut(s) 42, 286, 316, 321, 935
MboII GAAGA 6 cut(s) 52, 494, 749, 761, 912, 982
MfeI CAATTG 1 cut(s) 551
MflI RGATCY 2 cut(s) 42, 321
MhlI GDGCHC 4 cut(s) 466, 752, 1037, 1046
MluCI AATT 3 cut(s) 551, 728, 777
MluI ACGCGT 1 cut(s) 424
MlyI GAGTC 2 cut(s) 11, 710
MmeI TCCRAC 2 cut(s) 684, 873
MnlI CCTC 8 cut(s) 255, 322, 667, 735, 862, 895, 913, 943
MroNI GCCGGC 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 2 cut(s) 638, 780
MslI CAYNNNNRTG 2 cut(s) 371, 820
MspI CCGG 2 cut(s) 125, 1085
MspR9I CCNGG 3 cut(s) 249, 926, 1085
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 2 cut(s) 249, 926
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 1041, 1050, 1059
NaeI GCCGGC 1 cut(s) 126
NciI CCSGG 1 cut(s) 1085
NcoI CCATGG 1 cut(s) 978
NdeI CATATG 1 cut(s) 162
NdeII GATC 5 cut(s) 42, 286, 316, 321, 935
NgoMIV GCCGGC 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 1043
NmuCI GTSAC 3 cut(s) 373, 813, 943
NspI RCATGY 4 cut(s) 610, 628, 723, 965
NspV TTCGAA 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 820
PaeI GCATGC 1 cut(s) 723
PagI TCATGA 1 cut(s) 253
PaqCI CACCTGC 1 cut(s) 583
PdiI GCCGGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 2 cut(s) 20, 1017
PkrI GCNGC 4 cut(s) 202, 213, 268, 957
PleI GAGTC 2 cut(s) 11, 709
PpsI GAGTC 2 cut(s) 11, 709
PshBI ATTAAT 1 cut(s) 780
PsiI TTATAA 2 cut(s) 489, 1141
Psp124BI GAGCTC 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 247, 924
PspGI CCWGG 2 cut(s) 247, 924
PspN4I GGNNCC 1 cut(s) 1043
PspPI GGNCC 1 cut(s) 922
PsuI RGATCY 2 cut(s) 42, 321
PsyI GACNNNGTC 2 cut(s) 20, 1017
RsaI GTAC 4 cut(s) 498, 570, 986, 1010
RsaNI GTAC 4 cut(s) 497, 569, 985, 1009
RseI CAYNNNNRTG 2 cut(s) 371, 820
SacI GAGCTC 1 cut(s) 752
SaqAI TTAA 2 cut(s) 638, 780
SatI GCNGC 4 cut(s) 201, 212, 267, 956
Sau3AI GATC 5 cut(s) 42, 286, 316, 321, 935
Sau96I GGNCC 1 cut(s) 922
ScaI AGTACT 1 cut(s) 986
SchI GAGTC 2 cut(s) 11, 710
ScrFI CCNGG 3 cut(s) 249, 926, 1085
SduI GDGCHC 4 cut(s) 466, 752, 1037, 1046
SfaNI GCATC 1 cut(s) 1071
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 1 cut(s) 922
SmiMI CAYNNNNRTG 2 cut(s) 371, 820
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 723
Sse9I AATT 3 cut(s) 551, 728, 777
SsiI CCGC 1 cut(s) 380
SspMI CTAG 1 cut(s) 512
SstI GAGCTC 1 cut(s) 752
StyD4I CCNGG 3 cut(s) 247, 924, 1083
StyI CCWWGG 3 cut(s) 175, 978, 1037
TaaI ACNGT 2 cut(s) 568, 1017
TaqI TCGA 4 cut(s) 15, 73, 272, 938
TasI AATT 3 cut(s) 551, 728, 777
TatI WGTACW 3 cut(s) 568, 984, 1008
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 638, 780
Tru9I TTAA 2 cut(s) 638, 780
TseFI GTSAC 3 cut(s) 373, 813, 943
TseI GCWGC 4 cut(s) 200, 211, 266, 955
Tsp45I GTSAC 3 cut(s) 373, 813, 943
TspDTI ATGAA 2 cut(s) 425, 982
TspGWI ACGGA 1 cut(s) 10
Tth111I GACNNNGTC 2 cut(s) 20, 1017
VpaK11BI GGWCC 1 cut(s) 922
VspI ATTAAT 1 cut(s) 780
XceI RCATGY 4 cut(s) 610, 628, 723, 965
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 1 cut(s) 512
ZrmI AGTACT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.