Rroxscaffold_2G00148650

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001208
Physical Location & Seq
Forward (+)
86260047 .. 86264527
4481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148650.1

Sequence Viewer

Length: 1044 bp
ATGGCTGAATCAATCGACGCCGTCTCCCTTGATATGGAGAAGATCTATCTGGGTGGAAAGGAACATTTTATTCAAACTGGCTGTGGTTCTGTGTCTGTTATAGTTTATGGAGACCAAGATAAGCCGGCACTTATCACGTATCCTGATTTAGCTCTAAATCATATGTCTTGTTTCCAAGGGTTATTCTTTTGTCCTGAAGCAGCTTCTTTGCTGCTGCACAACTTCTGCATATACCATATTAGTCCTCCTGGTCATGAGTTAGGAGCTGCTTCGATTAGTCTTGATGATCCTGTGCCTTCTGCTGATGACTTGGCAGATCAGATCCTTGAGGTTCTAAACTTTTTCGGGCTTGGTGCAGTTATGTGCATGGGAGTGACAGCGGGTGCTTATATCCTTTCCTTATTTGCTATGAAATATAGGGAACGTGTTCTTGGTTTGATACTTGTATCCCCATTATGCAGAGCACCATCTTGGACTGAATGGTTTTATAATAAGGTGATATCAAACATGCTATATTTCTATGGCATGTGTGGTCTGTTAAAGGAGTGTTTACTTCAGCGTTACTTCAGTAAGGACGTTCGTGGTAGTGCTGAAGTTCCAGAGTCAGATATAGTTCAAGCATGCAGAAAATTATTGGAAGAGAGGCAGAGCTCAAATGTTTGGAGATTTCTTCAAGCAATTAATCGGAGACCTGACATAACTGAAGGATTGAAGTCACTAAGGTGTCGCACTCTCATTTTTGTTGGGGATAGCTCTCCTTTCCACTCTGAGGCTCTCCACATGACCTCAAAGTTGGATAGAAGATATAGTGCCTTAGTAGAGGTCCAGGCTTGTGGATCGATGGTGACAGAGGAGCAGCCACATGCAATGTTGATACCCATGGAGTACTTCTTCATGGGGTATGGTTTGTACAGACCGTGTCATTTCAGCGAGAGCCCAAGGAGCCCACTCAGCCCATCTTGCATCTCCCCTGAGCTTCTCTCCCCGGAAAGCATGGGTCTGAAGCTAAAACCAATCAAGACCCGTGTTTCGCTCGGATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

347

Amino Acids

38.73

Weight (kDa)

5.74

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 305 7.5e-116 Ndr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 1042
AciI CCGC 1 cut(s) 380
AclWI GGATC 3 cut(s) 281, 316, 844
AcuI CTGAAG 6 cut(s) 216, 539, 550, 612, 723, 1022
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 2 cut(s) 887, 911
AfiI CCNNNNNNNGG 2 cut(s) 34, 769
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 4 cut(s) 74, 617, 674, 712
AjnI CCWGG 2 cut(s) 247, 825
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AleI CACNNNNGTG 1 cut(s) 721
AluBI AGCT 7 cut(s) 152, 203, 266, 651, 753, 976, 1006
AluI AGCT 7 cut(s) 152, 203, 266, 651, 753, 976, 1006
Alw21I GWGCWC 2 cut(s) 466, 653
Alw26I GTCTC 3 cut(s) 28, 105, 682
AlwI GGATC 3 cut(s) 281, 316, 844
ApeKI GCWGC 5 cut(s) 200, 211, 214, 266, 856
ArsI GACNNNNNNTTYG 2 cut(s) 1012, 1044
AseI ATTAAT 1 cut(s) 681
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 1 cut(s) 823
AsuC2I CCSGG 1 cut(s) 986
AsuHPI GGTGA 2 cut(s) 508, 856
AvaII GGWCC 1 cut(s) 823
BanII GRGCYC 3 cut(s) 653, 938, 947
Bbv12I GWGCWC 2 cut(s) 466, 653
BbvI GCAGC 5 cut(s) 198, 201, 212, 253, 868
BccI CCATC 3 cut(s) 475, 835, 964
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 2 cut(s) 249, 827
BciVI GTATCC 2 cut(s) 150, 457
BcnI CCSGG 1 cut(s) 986
BcoDI GTCTC 3 cut(s) 28, 105, 682
BfuI GTATCC 2 cut(s) 150, 457
BglII AGATCT 1 cut(s) 42
BisI GCNGC 5 cut(s) 201, 212, 215, 267, 857
BlsI GCNGC 5 cut(s) 202, 213, 216, 268, 858
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 3 cut(s) 249, 827, 986
Bme18I GGWCC 1 cut(s) 823
BmgT120I GGNCC 1 cut(s) 823
BmiI GGNNCC 1 cut(s) 944
BmrFI CCNGG 3 cut(s) 249, 827, 986
BmsI GCATC 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 965, 997
Bpu10I CCTNAGC 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 986
Bsa29I ATCGAT 1 cut(s) 839
BsaAI YACGTR 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 105, 682
BsaJI CCNNGG 4 cut(s) 175, 879, 938, 984
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 769
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 2 cut(s) 249, 827
BseCI ATCGAT 1 cut(s) 839
BseDI CCNNGG 4 cut(s) 175, 879, 938, 984
BseLI CCNNNNNNNGG 2 cut(s) 34, 769
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 3 cut(s) 759, 963, 964
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 5 cut(s) 198, 201, 212, 253, 868
BsgI GTGCAG 2 cut(s) 200, 375
BshVI ATCGAT 1 cut(s) 839
BsiHKAI GWGCWC 2 cut(s) 466, 653
BsiSI CCGG 2 cut(s) 125, 986
BslI CCNNNNNNNGG 2 cut(s) 34, 769
BsmAI GTCTC 3 cut(s) 28, 105, 682
BsmBI CGTCTC 1 cut(s) 28
Bso31I GGTCTC 2 cut(s) 105, 682
Bsp1286I GDGCHC 4 cut(s) 466, 653, 938, 947
Bsp1407I TGTACA 1 cut(s) 909
Bsp143I GATC 5 cut(s) 42, 286, 316, 321, 836
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 1 cut(s) 380
BspCNI CTCAG 3 cut(s) 760, 963, 964
BspDI ATCGAT 1 cut(s) 839
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 944
BspPI GGATC 3 cut(s) 281, 316, 844
BspTNI GGTCTC 2 cut(s) 105, 682
BsrDI GCAATG 1 cut(s) 873
BsrFI RCCGGY 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 909
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 4 cut(s) 175, 879, 938, 984
BssMI GATC 5 cut(s) 42, 286, 316, 321, 836
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 3 cut(s) 175, 879, 938
Bst2UI CCWGG 2 cut(s) 249, 827
Bst4CI ACNGT 1 cut(s) 918
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 18
BstAUI TGTACA 1 cut(s) 909
BstBAI YACGTR 1 cut(s) 138
BstC8I GCNNGC 2 cut(s) 126, 622
BstDEI CTNAG 5 cut(s) 719, 768, 814, 950, 972
BstDSI CCRYGG 1 cut(s) 879
BstKTI GATC 5 cut(s) 45, 289, 319, 324, 839
BstMAI GTCTC 3 cut(s) 28, 105, 682
BstMBI GATC 5 cut(s) 42, 286, 316, 321, 836
BstMWI GCNNNNNNNGC 3 cut(s) 942, 951, 960
BstNI CCWGG 2 cut(s) 249, 827
BstNSI RCATGY 4 cut(s) 511, 529, 624, 866
BstSCI CCNGG 3 cut(s) 247, 825, 984
BstV1I GCAGC 5 cut(s) 198, 201, 212, 253, 868
BstX2I RGATCY 2 cut(s) 42, 321
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 2 cut(s) 42, 321
Bsu15I ATCGAT 1 cut(s) 839
BsuI GTATCC 2 cut(s) 150, 457
BsuTUI ATCGAT 1 cut(s) 839
BtgI CCRYGG 1 cut(s) 879
Cac8I GCNNGC 2 cut(s) 126, 622
CciI TCATGA 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 823
ClaI ATCGAT 1 cut(s) 839
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 886, 910
CviQI GTAC 2 cut(s) 886, 910
DdeI CTNAG 5 cut(s) 719, 768, 814, 950, 972
DpnI GATC 5 cut(s) 44, 288, 318, 323, 838
DpnII GATC 5 cut(s) 42, 286, 316, 321, 836
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Ecl136II GAGCTC 1 cut(s) 651
Eco130I CCWWGG 3 cut(s) 175, 879, 938
Eco24I GRGCYC 3 cut(s) 653, 938, 947
Eco31I GGTCTC 2 cut(s) 105, 682
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 823
Eco53kI GAGCTC 1 cut(s) 651
Eco57I CTGAAG 6 cut(s) 216, 539, 550, 612, 723, 1022
EcoICRI GAGCTC 1 cut(s) 651
EcoRII CCWGG 2 cut(s) 247, 825
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 3 cut(s) 175, 879, 938
EcoT38I GRGCYC 3 cut(s) 653, 938, 947
ErhI CCWWGG 3 cut(s) 175, 879, 938
Esp3I CGTCTC 1 cut(s) 28
FauI CCCGC 1 cut(s) 373
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 5 cut(s) 201, 212, 215, 267, 857
FriOI GRGCYC 3 cut(s) 653, 938, 947
Fsp4HI GCNGC 5 cut(s) 201, 212, 215, 267, 857
GluI GCNGC 5 cut(s) 201, 212, 215, 267, 857
HapII CCGG 2 cut(s) 125, 986
HgaI GACGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 18
HinfI GANTC 2 cut(s) 8, 602
HpaII CCGG 2 cut(s) 125, 986
HphI GGTGA 2 cut(s) 508, 856
Hpy166II GTNNAC 1 cut(s) 551
Hpy188I TCNGA 6 cut(s) 321, 607, 687, 769, 1002, 1037
Hpy188III TCNNGA 6 cut(s) 143, 194, 254, 281, 599, 1018
Hpy8I GTNNAC 1 cut(s) 551
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 306, 698
HpyCH4III ACNGT 1 cut(s) 918
HpyCH4IV ACGT 3 cut(s) 137, 424, 576
HpyCH4V TGCA 8 cut(s) 217, 228, 356, 366, 459, 624, 866, 963
HpyF10VI GCNNNNNNNGC 3 cut(s) 942, 951, 960
HpyF3I CTNAG 5 cut(s) 719, 768, 814, 950, 972
HpySE526I ACGT 3 cut(s) 137, 424, 576
Hsp92I GRCGYC 1 cut(s) 18
KroI GCCGGC 1 cut(s) 124
KroNI GCCGGC 1 cut(s) 126
Kzo9I GATC 5 cut(s) 42, 286, 316, 321, 836
LmnI GCTCC 3 cut(s) 263, 853, 942
Lsp1109I GCAGC 5 cut(s) 198, 201, 212, 253, 868
LweI GCATC 1 cut(s) 972
MaeII ACGT 3 cut(s) 137, 424, 576
MaeIII GTNAC 4 cut(s) 373, 560, 714, 844
MalI GATC 5 cut(s) 44, 288, 318, 323, 838
MboI GATC 5 cut(s) 42, 286, 316, 321, 836
MboII GAAGA 5 cut(s) 52, 650, 662, 813, 883
MflI RGATCY 2 cut(s) 42, 321
MhlI GDGCHC 4 cut(s) 466, 653, 938, 947
MluCI AATT 2 cut(s) 629, 678
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 774
MnlI CCTC 7 cut(s) 255, 322, 636, 763, 796, 814, 844
MroNI GCCGGC 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 2 cut(s) 539, 681
MslI CAYNNNNRTG 2 cut(s) 371, 721
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 2 cut(s) 125, 986
MspR9I CCNGG 3 cut(s) 249, 827, 986
MvaI CCWGG 2 cut(s) 249, 827
MwoI GCNNNNNNNGC 3 cut(s) 942, 951, 960
NaeI GCCGGC 1 cut(s) 126
NciI CCSGG 1 cut(s) 986
NcoI CCATGG 1 cut(s) 879
NdeI CATATG 1 cut(s) 162
NdeII GATC 5 cut(s) 42, 286, 316, 321, 836
NgoMIV GCCGGC 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 944
NmuCI GTSAC 3 cut(s) 373, 714, 844
NspI RCATGY 4 cut(s) 511, 529, 624, 866
OliI CACNNNNGTG 1 cut(s) 721
PaeI GCATGC 1 cut(s) 624
PagI TCATGA 1 cut(s) 253
PdiI GCCGGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 2 cut(s) 20, 918
PkrI GCNGC 5 cut(s) 202, 213, 216, 268, 858
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
Ppu21I YACGTR 1 cut(s) 138
PshBI ATTAAT 1 cut(s) 681
PsiI TTATAA 2 cut(s) 489, 1042
Psp124BI GAGCTC 1 cut(s) 653
Psp6I CCWGG 2 cut(s) 247, 825
PspGI CCWGG 2 cut(s) 247, 825
PspN4I GGNNCC 1 cut(s) 944
PspPI GGNCC 1 cut(s) 823
PsuI RGATCY 2 cut(s) 42, 321
PsyI GACNNNGTC 2 cut(s) 20, 918
RsaI GTAC 2 cut(s) 887, 911
RsaNI GTAC 2 cut(s) 886, 910
RseI CAYNNNNRTG 2 cut(s) 371, 721
SacI GAGCTC 1 cut(s) 653
SaqAI TTAA 2 cut(s) 539, 681
SatI GCNGC 5 cut(s) 201, 212, 215, 267, 857
Sau3AI GATC 5 cut(s) 42, 286, 316, 321, 836
Sau96I GGNCC 1 cut(s) 823
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 249, 827, 986
SduI GDGCHC 4 cut(s) 466, 653, 938, 947
SfaNI GCATC 1 cut(s) 972
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 2 cut(s) 371, 721
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 624
Sse9I AATT 2 cut(s) 629, 678
SsiI CCGC 1 cut(s) 380
SstI GAGCTC 1 cut(s) 653
StyD4I CCNGG 3 cut(s) 247, 825, 984
StyI CCWWGG 3 cut(s) 175, 879, 938
TaaI ACNGT 1 cut(s) 918
TaiI ACGT 3 cut(s) 140, 427, 579
TaqI TCGA 3 cut(s) 15, 272, 839
TasI AATT 2 cut(s) 629, 678
TatI WGTACW 2 cut(s) 885, 909
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 539, 681
Tru9I TTAA 2 cut(s) 539, 681
TseFI GTSAC 3 cut(s) 373, 714, 844
TseI GCWGC 5 cut(s) 200, 211, 214, 266, 856
Tsp45I GTSAC 3 cut(s) 373, 714, 844
TspDTI ATGAA 2 cut(s) 425, 883
Tth111I GACNNNGTC 2 cut(s) 20, 918
VpaK11BI GGWCC 1 cut(s) 823
VspI ATTAAT 1 cut(s) 681
XceI RCATGY 4 cut(s) 511, 529, 624, 866
XmnI GAANNNNTTC 1 cut(s) 426
ZrmI AGTACT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.