AT5G63050

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005312
Physical Location & Seq
Forward (+)
25292404 .. 25295020
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63050.1

Sequence Viewer

Length: 1038 bp
ATGGTGTTGGTAACCCATAAATTGCAGGGTTCACATGTCTTGTCTCCTTGGAGTTCGCCAACATGGACCAGAGGCTTGTGTTTAAAGAGACCTGTTACGACAGTGCACTTTGTTGAGAGGAAAGATAAGCATTTACGATTGAAGCAAAAATGTTGTTTTAGTTTAGGGTCCCCTTGCAACGGTGGATTGAGAGCAAAACCTTTCAGAGTTACATCTTTTAAAGGTGGCATCCAAAATAATGAATCAGGAGGAAGTGAGGGTGGGAAGAAAGTTACTAATAACTCTGTCAAACTATCTTACCGTTCAGATGATGACGAAAATAATGTGAATGGCTCCCCAAAGGCACAGAACACTTCGTTTTCATATACCTCGGAAACAGAGGATTCCATGACAGGGCAACCCGCTATTCAGAAACTATTCAAGAAATGGTTGACATTGTTGCGCACACAGTCACCTATTCAAGTGATTGACGAGACTTTAGGAGGAGAACAGGTTCCACAAACGACGAAGCAAGAAACTGAAACAGAAATTCGGAAAGCAGAAAGTCTCCAGAGTACCAAAAATACAGTTTGGTCCTGGTTTTGGAGTCTAGACGCAGCTATCAAGATTCCGTTGCTGTTATTTGTTCCAGCTTTCCTAGCTGTTTACGCGATCTGTGGTGCTGAAGTTACAAAGGAATTATCCCCTATGTGGGTGGTTGGCCCCTTGATCATCGCCCTTTATATAAAAATGTTCCAAGGACTATGTTCCCTTTACGCCTTCTGCTTCAACCAAACCATCAAAATGATAAGAAACCTACCATCGTATTATCTTGTAGCATACCAATACATTGCCGATGGAAAGCTCAAAGACGACGTTAAAGCTCTAGTGTTTAAACCAGTGGTGGCCATCAAGAACACAGACTACAAAGAGCTCACACGCACGAAGCTGAAACAGTTCCAAGAATGGATCATTGAGAAATACCTGGATTTTGTTGAATCTGTATGGCCTTATTACTGCAGAACTATCAGATTCCTGAAGAGGGCTAACCTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

345

Amino Acids

39.3

Weight (kDa)

9.41

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 443
AccII CGCG 1 cut(s) 650
AciI CCGC 1 cut(s) 402
AclWI GGATC 1 cut(s) 956
AcoI YGGCCR 1 cut(s) 885
AcsI RAATTY 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 684, 1037
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 6 cut(s) 179, 393, 582, 690, 691, 1021
AflIII ACRYGT 1 cut(s) 34
AgsI TTSAA 5 cut(s) 142, 421, 461, 769, 977
AjnI CCWGG 2 cut(s) 575, 963
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
AluBI AGCT 7 cut(s) 599, 632, 641, 844, 863, 913, 928
AluI AGCT 7 cut(s) 599, 632, 641, 844, 863, 913, 928
Alw21I GWGCWC 2 cut(s) 108, 915
Alw26I GTCTC 4 cut(s) 48, 82, 467, 551
Alw44I GTGCAC 1 cut(s) 104
AlwI GGATC 1 cut(s) 956
AoxI GGCC 3 cut(s) 700, 885, 986
ApaLI GTGCAC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 1 cut(s) 528
Asp700I GAANNNNTTC 3 cut(s) 416, 492, 935
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 4 cut(s) 66, 168, 573, 701
AsuHPI GGTGA 1 cut(s) 444
AvaII GGWCC 3 cut(s) 66, 168, 573
BaeGI GKGCMC 1 cut(s) 108
BalI TGGCCA 1 cut(s) 887
BanII GRGCYC 1 cut(s) 915
Bbv12I GWGCWC 2 cut(s) 108, 915
BbvI GCAGC 1 cut(s) 608
BccI CCATC 4 cut(s) 785, 808, 830, 896
BciT130I CCWGG 2 cut(s) 577, 965
BclI TGATCA 1 cut(s) 708
BcoDI GTCTC 4 cut(s) 48, 82, 467, 551
BfaI CTAG 3 cut(s) 590, 638, 866
BfmI CTRYAG 1 cut(s) 997
BisI GCNGC 1 cut(s) 597
BlsI GCNGC 1 cut(s) 598
Bme1390I CCNGG 2 cut(s) 577, 965
Bme18I GGWCC 3 cut(s) 66, 168, 573
BmgT120I GGNCC 4 cut(s) 66, 168, 573, 701
BmiI GGNNCC 5 cut(s) 169, 170, 334, 495, 703
BmrFI CCNGG 2 cut(s) 577, 965
BmsI GCATC 1 cut(s) 237
BpmI CTGGAG 1 cut(s) 533
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 47, 369, 736
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 6 cut(s) 179, 393, 582, 690, 691, 1021
Bse1I ACTGG 1 cut(s) 878
Bse3DI GCAATG 1 cut(s) 828
BseBI CCWGG 2 cut(s) 577, 965
BseDI CCNNGG 3 cut(s) 47, 369, 736
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 6 cut(s) 179, 393, 582, 690, 691, 1021
BseMI GCAATG 1 cut(s) 828
BseNI ACTGG 1 cut(s) 878
BseRI GAGGAG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 1 cut(s) 608
Bsh1236I CGCG 1 cut(s) 650
BshFI GGCC 3 cut(s) 702, 887, 988
BsiHKAI GWGCWC 2 cut(s) 108, 915
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 6 cut(s) 179, 393, 582, 690, 691, 1021
BsmAI GTCTC 4 cut(s) 48, 82, 467, 551
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 3 cut(s) 702, 887, 988
Bso31I GGTCTC 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 108, 915
Bsp143I GATC 3 cut(s) 651, 708, 948
BspACI CCGC 1 cut(s) 402
BspANI GGCC 3 cut(s) 702, 887, 988
BspFNI CGCG 1 cut(s) 650
BspLI GGNNCC 5 cut(s) 169, 170, 334, 495, 703
BspMAI CTGCAG 1 cut(s) 1001
BspPI GGATC 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 828
BsrI ACTGG 1 cut(s) 878
BssECI CCNNGG 3 cut(s) 47, 369, 736
BssMI GATC 3 cut(s) 651, 708, 948
BssT1I CCWWGG 2 cut(s) 47, 736
Bst2UI CCWGG 2 cut(s) 577, 965
Bst4CI ACNGT 6 cut(s) 103, 182, 302, 450, 568, 936
Bst6I CTCTTC 1 cut(s) 1013
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 650
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 3 cut(s) 654, 711, 951
BstMAI GTCTC 4 cut(s) 48, 82, 467, 551
BstMBI GATC 3 cut(s) 651, 708, 948
BstMWI GCNNNNNNNGC 2 cut(s) 638, 647
BstNI CCWGG 2 cut(s) 577, 965
BstNSI RCATGY 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 575, 963
BstSFI CTRYAG 1 cut(s) 997
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 650
BstV1I GCAGC 1 cut(s) 608
BsuRI GGCC 3 cut(s) 702, 887, 988
BtgZI GCGATG 1 cut(s) 697
BtsCI GGATG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 108, 885
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 4 cut(s) 66, 168, 573, 701
CseI GACGC 1 cut(s) 602
Csp6I GTAC 1 cut(s) 555
CviAII CATG 3 cut(s) 35, 63, 388
CviQI GTAC 1 cut(s) 555
DpnI GATC 3 cut(s) 653, 710, 950
DpnII GATC 3 cut(s) 651, 708, 948
DraI TTTAAA 3 cut(s) 84, 220, 874
EaeI YGGCCR 1 cut(s) 885
Eam1104I CTCTTC 1 cut(s) 1013
EarI CTCTTC 1 cut(s) 1013
Ecl136II GAGCTC 1 cut(s) 913
Eco130I CCWWGG 2 cut(s) 47, 736
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 3 cut(s) 66, 168, 573
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 2 cut(s) 684, 1037
Eco91I GGTNACC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 913
EcoO109I RGGNCCY 1 cut(s) 168
EcoO65I GGTNACC 1 cut(s) 10
EcoRII CCWGG 2 cut(s) 575, 963
EcoT14I CCWWGG 2 cut(s) 47, 736
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 2 cut(s) 47, 736
FaeI CATG 3 cut(s) 38, 66, 391
FaqI GGGAC 1 cut(s) 154
FatI CATG 3 cut(s) 34, 62, 387
FauI CCCGC 1 cut(s) 409
FbaI TGATCA 1 cut(s) 708
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 1 cut(s) 597
FspBI CTAG 3 cut(s) 590, 638, 866
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 1 cut(s) 443
GluI GCNGC 1 cut(s) 597
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 3 cut(s) 702, 887, 988
HgaI GACGC 1 cut(s) 602
HhaI GCGC 1 cut(s) 444
Hin1II CATG 3 cut(s) 38, 66, 391
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 6 cut(s) 242, 383, 586, 607, 977, 1011
HphI GGTGA 1 cut(s) 444
Hpy166II GTNNAC 4 cut(s) 32, 106, 432, 646
Hpy188I TCNGA 6 cut(s) 206, 307, 373, 411, 534, 1010
Hpy188III TCNNGA 7 cut(s) 246, 421, 550, 590, 604, 892, 1015
Hpy8I GTNNAC 4 cut(s) 32, 106, 432, 646
Hpy99I CGWCG 2 cut(s) 508, 857
HpyAV CCTTC 1 cut(s) 769
HpyCH4III ACNGT 6 cut(s) 103, 182, 302, 450, 568, 936
HpyCH4IV ACGT 1 cut(s) 855
HpyCH4V TGCA 4 cut(s) 25, 106, 177, 999
HpyF10VI GCNNNNNNNGC 2 cut(s) 638, 647
HpySE526I ACGT 1 cut(s) 855
Hsp92II CATG 3 cut(s) 38, 66, 391
HspAI GCGC 1 cut(s) 442
KflI GGGWCCC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 3 cut(s) 651, 708, 948
LmnI GCTCC 1 cut(s) 338
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 1 cut(s) 237
MaeI CTAG 3 cut(s) 590, 638, 866
MaeII ACGT 1 cut(s) 855
MaeIII GTNAC 6 cut(s) 10, 94, 208, 271, 450, 667
MalI GATC 3 cut(s) 653, 710, 950
MboI GATC 3 cut(s) 651, 708, 948
MboII GAAGA 2 cut(s) 277, 1030
MhlI GDGCHC 2 cut(s) 108, 915
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 3 cut(s) 20, 528, 677
MluNI TGGCCA 1 cut(s) 887
MlyI GAGTC 1 cut(s) 595
MnlI CCTC 8 cut(s) 65, 111, 242, 250, 373, 379, 476, 1014
Mox20I TGGCCA 1 cut(s) 887
MroXI GAANNNNTTC 3 cut(s) 416, 492, 935
MscI TGGCCA 1 cut(s) 887
MseI TTAA 4 cut(s) 83, 219, 858, 873
MslI CAYNNNNRTG 1 cut(s) 782
Msp20I TGGCCA 1 cut(s) 887
MspR9I CCNGG 2 cut(s) 577, 965
MssI GTTTAAAC 1 cut(s) 874
MvaI CCWGG 2 cut(s) 577, 965
MvnI CGCG 1 cut(s) 650
MwoI GCNNNNNNNGC 2 cut(s) 638, 647
NdeII GATC 3 cut(s) 651, 708, 948
NlaIII CATG 3 cut(s) 38, 66, 391
NlaIV GGNNCC 5 cut(s) 169, 170, 334, 495, 703
NmuCI GTSAC 1 cut(s) 450
NsbI TGCGCA 1 cut(s) 443
NspI RCATGY 1 cut(s) 38
PciI ACATGT 1 cut(s) 34
PdmI GAANNNNTTC 3 cut(s) 416, 492, 935
PfeI GAWTC 5 cut(s) 242, 383, 607, 977, 1011
PkrI GCNGC 1 cut(s) 598
PleI GAGTC 1 cut(s) 594
PmeI GTTTAAAC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 594
PpuMI RGGWCCY 1 cut(s) 168
PscI ACATGT 1 cut(s) 34
Psp124BI GAGCTC 1 cut(s) 915
Psp5II RGGWCCY 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 575, 963
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 575, 963
PspN4I GGNNCC 5 cut(s) 169, 170, 334, 495, 703
PspPI GGNCC 4 cut(s) 66, 168, 573, 701
PspPPI RGGWCCY 1 cut(s) 168
PsrI GAACNNNNNNTAC 2 cut(s) 887, 919
PstI CTGCAG 1 cut(s) 1001
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 915
SaqAI TTAA 4 cut(s) 83, 219, 858, 873
SatI GCNGC 1 cut(s) 597
Sau3AI GATC 3 cut(s) 651, 708, 948
Sau96I GGNCC 4 cut(s) 66, 168, 573, 701
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 2 cut(s) 577, 965
SduI GDGCHC 2 cut(s) 108, 915
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 997
SinI GGWCC 3 cut(s) 66, 168, 573
SmiMI CAYNNNNRTG 1 cut(s) 782
Sse9I AATT 3 cut(s) 20, 528, 677
SsiI CCGC 1 cut(s) 402
SspMI CTAG 3 cut(s) 590, 638, 866
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 2 cut(s) 575, 963
StyI CCWWGG 2 cut(s) 47, 736
TaaI ACNGT 6 cut(s) 103, 182, 302, 450, 568, 936
TaiI ACGT 1 cut(s) 858
TasI AATT 3 cut(s) 20, 528, 677
TfiI GAWTC 5 cut(s) 242, 383, 607, 977, 1011
Tru1I TTAA 4 cut(s) 83, 219, 858, 873
Tru9I TTAA 4 cut(s) 83, 219, 858, 873
TscAI CASTG 2 cut(s) 108, 885
TseFI GTSAC 1 cut(s) 450
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 1 cut(s) 450
TspDTI ATGAA 2 cut(s) 255, 351
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 2 cut(s) 108, 885
VneI GTGCAC 1 cut(s) 104
VpaK11BI GGWCC 3 cut(s) 66, 168, 573
XapI RAATTY 1 cut(s) 528
XbaI TCTAGA 1 cut(s) 589
XceI RCATGY 1 cut(s) 38
XmnI GAANNNNTTC 3 cut(s) 416, 492, 935
XspI CTAG 3 cut(s) 590, 638, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.