pycom14g14750

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005312
Physical Location & Seq
Forward (+)
17442494 .. 17444121
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14750.1

Sequence Viewer

Length: 1020 bp
ATGGCTTTGGTTACCCATCAAATGCAGGGTTCTTATGTGACTTATCCCTCTAGGCCTTTGTCATGGAGCAAAGGGATGAAGTTGAAGCAGTGTGTAACAACACATCAAATGGTCGGGAGGACAGAGAAGTGCTTTATGCTAAAGCGCAACCTTTGTTTAAGTGTAGGGGCTTCTCTCATGCGGGGACCAAAAGTTAAACCTTTCAAAGTTTCAGCCTTCAAGGGCAGTGCTCAGAATGATAAATCTGGAGGTAGAACAAATGGATCAAAGCTTCCCAAGAATTCGGTCAAACTAAAAGAGAATGAGGATACTATCACGGGATCTCCTCAGGCAAATGATATTCCTCTTTCTTATGCCTCTGAAGCAGATGAGAGAAATGCATCTTCCCCCGTCATTCATAATTTATTTAAGAAATGGTTGAGAATGCTTCGAACACAATCACCAAGTGAAGTAGAAGATGGGCTTTTGGGGGAAGAACCGCCTCCAATGGAGACATCAGAAACTAAGCATGAGATTCAAAACAAGGAAAATGATGGGATTCTATGGACGGTTTGGTGCAATTTTCTGGGTCTAAATGCTACAGTAAAGATACCCTTACTTATATTCATCCCCATGTACCTGGCAGTTAATATGATTCATGGGGCCGAAGTTTCAAAGGAGTTGACGCCTTTGTGGGTTTTAGGACCCCTCATTGTAGCACTCTACATCAAGATGCTTCAGTGGTTGTGTGCACTCTATGTTTTCAGCTTCAAGCAGACTGTTAAAATAATCAAGAATTTACCCACTTACTACATGGTGGCCTATACATACATTGCACATGGGAAGCTTAAAGAGGAAATACGGACTCGTTTCTGGCAACCTTTACTGAACATTAAGAACTTAGACTACAAGAAATTATCCAGAAGAAAGCTGAAAGCACTGCAAGGGTTGATAATCGAGAGATACCTTGATTTCGTGGAATCAATATGGCCTTATTACTGCAGAACAATCAGATTCCTAAAGAGGGCTAATCTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

38.88

Weight (kDa)

9.68

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 181, 479
AclWI GGATC 2 cut(s) 271, 328
AcsI RAATTY 2 cut(s) 280, 775
AcuI CTGAAG 2 cut(s) 381, 701
AcyI GRCGYC 1 cut(s) 665
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 1003
AgsI TTSAA 6 cut(s) 85, 205, 220, 518, 654, 751
AjnI CCWGG 1 cut(s) 618
AluBI AGCT 4 cut(s) 271, 747, 826, 910
AluI AGCT 4 cut(s) 271, 747, 826, 910
Alw21I GWGCWC 2 cut(s) 232, 733
Alw26I GTCTC 1 cut(s) 485
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 2 cut(s) 271, 328
AoxI GGCC 4 cut(s) 53, 642, 798, 968
ApaLI GTGCAC 1 cut(s) 729
ApoI RAATTY 2 cut(s) 280, 775
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 185, 642, 683
AsuHPI GGTGA 1 cut(s) 432
AsuII TTCGAA 1 cut(s) 430
AvaII GGWCC 2 cut(s) 185, 683
AxyI CCTNAGG 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 733
Bbv12I GWGCWC 2 cut(s) 232, 733
BccI CCATC 3 cut(s) 24, 452, 527
BciT130I CCWGG 1 cut(s) 620
BciVI GTATCC 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 2 cut(s) 579, 979
BfuI GTATCC 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 620
Bme18I GGWCC 2 cut(s) 185, 683
BmgT120I GGNCC 3 cut(s) 185, 642, 683
BmiI GGNNCC 3 cut(s) 186, 643, 685
BmrFI CCNGG 1 cut(s) 620
BmsI GCATC 2 cut(s) 389, 702
BpmI CTGGAG 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 430
BsaHI GRCGYC 1 cut(s) 665
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 1003
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 1 cut(s) 620
BseGI GGATG 2 cut(s) 81, 606
BseLI CCNNNNNNNGG 1 cut(s) 1003
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 2 cut(s) 245, 341
BseRI GAGGAG 1 cut(s) 315
BseSI GKGCMC 1 cut(s) 733
BshFI GGCC 4 cut(s) 55, 644, 800, 970
BsiHKAI GWGCWC 2 cut(s) 232, 733
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 1003
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 198
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 4 cut(s) 55, 644, 800, 970
Bsp119I TTCGAA 1 cut(s) 430
Bsp1286I GDGCHC 2 cut(s) 232, 733
Bsp143I GATC 2 cut(s) 263, 320
BspACI CCGC 2 cut(s) 181, 479
BspANI GGCC 4 cut(s) 55, 644, 800, 970
BspCNI CTCAG 2 cut(s) 244, 340
BspLI GGNNCC 3 cut(s) 186, 643, 685
BspMAI CTGCAG 1 cut(s) 983
BspPI GGATC 2 cut(s) 271, 328
BspT104I TTCGAA 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 810
BssMI GATC 2 cut(s) 263, 320
BssNI GRCGYC 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 3 cut(s) 550, 583, 760
BstACI GRCGYC 1 cut(s) 665
BstBI TTCGAA 1 cut(s) 430
BstDEI CTNAG 4 cut(s) 231, 327, 504, 880
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 2 cut(s) 81, 606
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 2 cut(s) 266, 323
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 2 cut(s) 263, 320
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 620
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 618
BstSFI CTRYAG 2 cut(s) 579, 979
BstSLI GKGCMC 1 cut(s) 733
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 619
BstYI RGATCY 1 cut(s) 320
Bsu36I CCTNAGG 1 cut(s) 327
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 4 cut(s) 55, 644, 800, 970
BtsCI GGATG 2 cut(s) 81, 606
BtsI GCAGTG 3 cut(s) 95, 232, 917
BtsIMutI CAGTG 4 cut(s) 95, 232, 725, 917
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 3 cut(s) 185, 642, 683
CseI GACGC 1 cut(s) 673
Csp6I GTAC 1 cut(s) 616
CviAII CATG 7 cut(s) 63, 178, 509, 613, 638, 793, 818
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 4 cut(s) 231, 327, 504, 880
DpnI GATC 2 cut(s) 265, 322
DpnII GATC 2 cut(s) 263, 320
DraIII CACNNNGTG 1 cut(s) 446
Eco147I AGGCCT 1 cut(s) 55
Eco47I GGWCC 2 cut(s) 185, 683
Eco57I CTGAAG 2 cut(s) 381, 701
Eco81I CCTNAGG 1 cut(s) 327
Eco91I GGTNACC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 683
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 382
FaeI CATG 7 cut(s) 66, 181, 512, 616, 641, 796, 821
FalI AAGNNNNNCTT 4 cut(s) 447, 479, 578, 610
FaqI GGGAC 1 cut(s) 198
FatI CATG 7 cut(s) 62, 177, 508, 612, 637, 792, 817
FauI CCCGC 1 cut(s) 174
FokI GGATG 2 cut(s) 88, 593
FspBI CTAG 1 cut(s) 51
GlaI GCGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 4 cut(s) 55, 644, 800, 970
HgaI GACGC 1 cut(s) 673
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 665
Hin1II CATG 7 cut(s) 66, 181, 512, 616, 641, 796, 821
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 2 cut(s) 269, 824
HinfI GANTC 6 cut(s) 514, 538, 634, 844, 959, 993
HphI GGTGA 1 cut(s) 432
Hpy166II GTNNAC 2 cut(s) 663, 731
Hpy188I TCNGA 4 cut(s) 234, 361, 499, 992
Hpy188III TCNNGA 6 cut(s) 115, 246, 709, 772, 900, 937
Hpy8I GTNNAC 2 cut(s) 663, 731
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 3 cut(s) 550, 583, 760
HpyCH4V TGCA 7 cut(s) 25, 380, 558, 731, 815, 922, 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 4 cut(s) 231, 327, 504, 880
Hsp92I GRCGYC 1 cut(s) 665
Hsp92II CATG 7 cut(s) 66, 181, 512, 616, 641, 796, 821
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 263, 320
LmnI GCTCC 1 cut(s) 66
LpnPI CCDG 8 cut(s) 11, 231, 314, 551, 605, 632, 838, 913
LweI GCATC 2 cut(s) 389, 702
MaeI CTAG 1 cut(s) 51
MaeIII GTNAC 3 cut(s) 10, 37, 94
MalI GATC 2 cut(s) 265, 322
MboI GATC 2 cut(s) 263, 320
MboII GAAGA 4 cut(s) 375, 467, 485, 915
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 2 cut(s) 232, 733
MluCI AATT 5 cut(s) 280, 400, 559, 775, 893
MlyI GAGTC 1 cut(s) 838
Mph1103I ATGCAT 1 cut(s) 382
MseI TTAA 7 cut(s) 158, 195, 408, 627, 762, 828, 873
MslI CAYNNNNRTG 2 cut(s) 611, 710
MspR9I CCNGG 1 cut(s) 620
Mva1269I GAATGC 1 cut(s) 429
MvaI CCWGG 1 cut(s) 620
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 2 cut(s) 263, 320
NlaIII CATG 7 cut(s) 66, 181, 512, 616, 641, 796, 821
NlaIV GGNNCC 3 cut(s) 186, 643, 685
NmuCI GTSAC 1 cut(s) 37
NsiI ATGCAT 1 cut(s) 382
NspV TTCGAA 1 cut(s) 430
PceI AGGCCT 1 cut(s) 55
PctI GAATGC 1 cut(s) 429
PfeI GAWTC 5 cut(s) 514, 538, 634, 959, 993
PleI GAGTC 1 cut(s) 838
PpsI GAGTC 1 cut(s) 838
PpuMI RGGWCCY 1 cut(s) 683
Psp5II RGGWCCY 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 618
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 3 cut(s) 186, 643, 685
PspPI GGNCC 3 cut(s) 185, 642, 683
PspPPI RGGWCCY 1 cut(s) 683
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PstI CTGCAG 1 cut(s) 983
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 2 cut(s) 611, 710
SaqAI TTAA 7 cut(s) 158, 195, 408, 627, 762, 828, 873
Sau3AI GATC 2 cut(s) 263, 320
Sau96I GGNCC 3 cut(s) 185, 642, 683
SchI GAGTC 1 cut(s) 838
ScrFI CCNGG 1 cut(s) 620
SduI GDGCHC 2 cut(s) 232, 733
SfaNI GCATC 2 cut(s) 389, 702
SfcI CTRYAG 2 cut(s) 579, 979
SfuI TTCGAA 1 cut(s) 430
SinI GGWCC 2 cut(s) 185, 683
SmiMI CAYNNNNRTG 2 cut(s) 611, 710
Sse9I AATT 5 cut(s) 280, 400, 559, 775, 893
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 2 cut(s) 181, 479
SspMI CTAG 1 cut(s) 51
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 1 cut(s) 618
TaaI ACNGT 3 cut(s) 550, 583, 760
TaqI TCGA 2 cut(s) 430, 936
TaqII GACCGA 1 cut(s) 274
TasI AATT 5 cut(s) 280, 400, 559, 775, 893
TfiI GAWTC 5 cut(s) 514, 538, 634, 959, 993
Tru1I TTAA 7 cut(s) 158, 195, 408, 627, 762, 828, 873
Tru9I TTAA 7 cut(s) 158, 195, 408, 627, 762, 828, 873
TscAI CASTG 4 cut(s) 95, 232, 725, 924
TseFI GTSAC 1 cut(s) 37
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 4 cut(s) 92, 386, 595, 626
TspGWI ACGGA 1 cut(s) 856
TspRI CASTG 4 cut(s) 95, 232, 725, 924
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 2 cut(s) 185, 683
XapI RAATTY 2 cut(s) 280, 775
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.