AT5G66080

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002066
Physical Location & Seq
Reverse (-)
26423244 .. 26425448
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66080.2

Sequence Viewer

Length: 1158 bp
ATGTTATCCCTTTTCTTCAACTTTTTGACCTCTTGTCTCTGGCCCTCTTCTTCCACCACTTCCCACACTTATTCTGATTCCAAAGGCAAACAAGACGGCCTTCTCTGGTACAAAGACTCTGCTCACCACCTTTTTGGTGATTTCTCCATGGCCGTTGTCCAGGCCAACAACCTCCTCGAGGATCAGAGCCAGGTCGAATCCGGTCCTTTGACCACCCTCTCTTCTTCTGGTCCCTATGGAACCTTTGTTGGCGTCTATGACGGCCACGGAGGCCCTGAGACCTCCCGTTTTGTCAACGATCATCTCTTTCACCATCTCAAGAGATTCGCAGCGGAGCAGGATTCCATGTCTGTGGATGTGATAAGAAAGGCCTATGAGGCAACCGAAGAAGGGTTTCTAGGGGTGGTGGCCAAGCAGTGGGCAGTGAAGCCACATATAGCAGCGGTTGGCTCTTGTTGCCTGATCGGTGTGGTCTGCGATGGGAAGCTTTACGTAGCTAACGTTGGGGACTCCCGTGCGGTTTTGGGAAAGGTCATCAAGGCCACAGGTGAGGTTAATGCGCTTCAGCTCTCAGCTGAGCATAACGTGAGCATAGAGTCTGTGAGGCAGGAAATGCACTCCTTACACCCTGATGACTCCCATATTGTCGTCTTAAAACACAATGTCTGGCGTGTTAAGGGCATTATTCAGGTATCACGATCCATAGGAGACGTGTATCTTAAGAAATCGGAGTTCAACAAGGAACCTTTGTACACAAAGTACCGATTGCGGGAGCCAATGAAAAGACCTATCTTGAGTTGGGAACCGTCGATAACCGTGCATGATCTCCAACCAGACGATCAGTTTCTGATATTTGCATCAGACGGACTTTGGGAGCAGCTTAGCAACCAAGAGGCTGTTGAAATCGTTCAGAACCACCCAAGAAACGGGATAGCGAGGAGACTGGTGAAAGCTGCGCTGCAGGAGGCAGCAAAGAAAAGAGAGATGAGATATTCTGATCTCAACAAGATTGAGAGAGGAGTCAGGAGGCATTTCCATGACGACATCACTGTGGTCGTCCTCTTCCTGGACACCAATCTGCTCAGCAGAGCCTCCTCTCTTAAAACCCCTTCTGTTTCTATCCGTGGTGGTGGTATCACTTTACCTAAGAAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

385

Amino Acids

42.96

Weight (kDa)

7.77

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 17 - 221 1.7e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 176
AccB7I CCANNNNNTGG 1 cut(s) 417
AciI CCGC 4 cut(s) 332, 443, 518, 769
AclI AACGTT 1 cut(s) 501
AclWI GGATC 2 cut(s) 189, 693
AcoI YGGCCR 3 cut(s) 150, 262, 408
AcuI CTGAAG 1 cut(s) 548
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 3 cut(s) 110, 752, 761
AfiI CCNNNNNNNGG 6 cut(s) 178, 390, 417, 769, 926, 1066
AflII CTTAAG 1 cut(s) 719
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 3 cut(s) 19, 736, 902
AhdI GACNNNNNGTC 1 cut(s) 33
AjiI CACGTC 1 cut(s) 712
AjnI CCWGG 3 cut(s) 159, 189, 1065
AluBI AGCT 6 cut(s) 487, 497, 568, 575, 880, 953
AluI AGCT 6 cut(s) 487, 497, 568, 575, 880, 953
Alw26I GTCTC 4 cut(s) 41, 272, 702, 934
AlwI GGATC 2 cut(s) 189, 693
AlwNI CAGNNNCTG 1 cut(s) 847
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 9 cut(s) 41, 97, 150, 162, 262, 271, 369, 408, 540
ApeKI GCWGC 6 cut(s) 329, 440, 877, 953, 958, 968
Asp700I GAANNNNTTC 2 cut(s) 393, 906
AspLEI GCGC 2 cut(s) 562, 958
AspS9I GGNCC 4 cut(s) 42, 203, 230, 272
AsuHPI GGTGA 5 cut(s) 116, 149, 302, 560, 958
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 2 cut(s) 203, 230
BalI TGGCCA 1 cut(s) 410
BbvI GCAGC 6 cut(s) 341, 452, 889, 940, 945, 980
BccI CCATC 2 cut(s) 321, 473
BceAI ACGGC 3 cut(s) 112, 137, 277
BcgI CGANNNNNNTGC 2 cut(s) 799, 833
BciT130I CCWGG 3 cut(s) 161, 191, 1067
BcoDI GTCTC 4 cut(s) 41, 272, 702, 934
BfaI CTAG 1 cut(s) 398
BfmI CTRYAG 1 cut(s) 959
BfrI CTTAAG 1 cut(s) 719
BglI GCCNNNNNGGC 2 cut(s) 270, 377
BisI GCNGC 6 cut(s) 330, 441, 878, 954, 959, 969
BlpI GCTNAGC 3 cut(s) 576, 881, 1082
BlsI GCNGC 6 cut(s) 331, 442, 879, 955, 960, 970
Bme1390I CCNGG 3 cut(s) 161, 191, 1067
Bme18I GGWCC 2 cut(s) 203, 230
BmeRI GACNNNNNGTC 1 cut(s) 33
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 4 cut(s) 42, 203, 230, 272
BmiI GGNNCC 5 cut(s) 232, 241, 744, 774, 804
BmrFI CCNGG 3 cut(s) 161, 191, 1067
BmsI GCATC 1 cut(s) 866
Bpu1102I GCTNAGC 3 cut(s) 576, 881, 1082
BpuEI CTTGAG 2 cut(s) 302, 814
BsaAI YACGTR 1 cut(s) 493
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 3 cut(s) 147, 265, 1123
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 6 cut(s) 178, 390, 417, 769, 926, 1066
Bse1I ACTGG 1 cut(s) 948
BseBI CCWGG 3 cut(s) 161, 191, 1067
BseDI CCNNGG 3 cut(s) 147, 265, 1123
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 6 cut(s) 178, 390, 417, 769, 926, 1066
BseMII CTCAG 4 cut(s) 267, 567, 585, 1096
BseNI ACTGG 1 cut(s) 948
BseRI GAGGAG 4 cut(s) 164, 952, 1032, 1084
BseXI GCAGC 6 cut(s) 341, 452, 889, 940, 945, 980
BshFI GGCC 9 cut(s) 43, 99, 152, 164, 264, 273, 371, 410, 542
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 2 cut(s) 216, 521
BslI CCNNNNNNNGG 6 cut(s) 178, 390, 417, 769, 926, 1066
BsmAI GTCTC 4 cut(s) 41, 272, 702, 934
BsmBI CGTCTC 1 cut(s) 702
BsmFI GGGAC 2 cut(s) 216, 521
BsnI GGCC 9 cut(s) 43, 99, 152, 164, 264, 273, 371, 410, 542
Bso31I GGTCTC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 176
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
Bsp1720I GCTNAGC 3 cut(s) 576, 881, 1082
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 4 cut(s) 332, 443, 518, 769
BspANI GGCC 9 cut(s) 43, 99, 152, 164, 264, 273, 371, 410, 542
BspCNI CTCAG 4 cut(s) 268, 568, 584, 1095
BspLI GGNNCC 5 cut(s) 232, 241, 744, 774, 804
BspMAI CTGCAG 1 cut(s) 963
BspPI GGATC 2 cut(s) 189, 693
BspTI CTTAAG 1 cut(s) 719
BspTNI GGTCTC 1 cut(s) 272
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 1 cut(s) 948
BssECI CCNNGG 3 cut(s) 147, 265, 1123
BssMI GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 3 cut(s) 161, 191, 1067
Bst4CI ACNGT 3 cut(s) 807, 817, 1051
Bst6I CTCTTC 3 cut(s) 52, 226, 1067
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 719
BstAPI GCANNNNNTGC 1 cut(s) 613
BstAUI TGTACA 1 cut(s) 750
BstBAI YACGTR 1 cut(s) 493
BstDEI CTNAG 6 cut(s) 276, 571, 576, 881, 1082, 1146
BstDSI CCRYGG 3 cut(s) 147, 265, 1123
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 361
BstHHI GCGC 2 cut(s) 562, 958
BstKTI GATC 7 cut(s) 184, 301, 465, 701, 826, 841, 1000
BstMAI GTCTC 4 cut(s) 41, 272, 702, 934
BstMBI GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
BstMWI GCNNNNNNNGC 4 cut(s) 270, 377, 456, 613
BstNI CCWGG 3 cut(s) 161, 191, 1067
BstSCI CCNGG 3 cut(s) 159, 189, 1065
BstSFI CTRYAG 1 cut(s) 959
BstSNI TACGTA 1 cut(s) 493
BstV1I GCAGC 6 cut(s) 341, 452, 889, 940, 945, 980
BstXI CCANNNNNNTGG 2 cut(s) 134, 352
BsuRI GGCC 9 cut(s) 43, 99, 152, 164, 264, 273, 371, 410, 542
BtgI CCRYGG 3 cut(s) 147, 265, 1123
BtgZI GCGATG 1 cut(s) 492
BtrI CACGTC 1 cut(s) 712
BtsCI GGATG 1 cut(s) 361
BtsI GCAGTG 2 cut(s) 422, 429
BtsIMutI CAGTG 3 cut(s) 422, 429, 1047
CaiI CAGNNNCTG 1 cut(s) 847
CfoI GCGC 2 cut(s) 562, 958
Cfr13I GGNCC 4 cut(s) 42, 203, 230, 272
CseI GACGC 1 cut(s) 241
Csp6I GTAC 3 cut(s) 109, 751, 760
CviAII CATG 4 cut(s) 148, 346, 821, 1037
CviQI GTAC 3 cut(s) 109, 751, 760
DdeI CTNAG 6 cut(s) 276, 571, 576, 881, 1082, 1146
DpnI GATC 7 cut(s) 183, 300, 464, 700, 825, 840, 999
DpnII GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
DriI GACNNNNNGTC 1 cut(s) 33
EaeI YGGCCR 3 cut(s) 150, 262, 408
Eam1104I CTCTTC 3 cut(s) 52, 226, 1067
Eam1105I GACNNNNNGTC 1 cut(s) 33
EarI CTCTTC 3 cut(s) 52, 226, 1067
Eco105I TACGTA 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 147
Eco147I AGGCCT 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 272
Eco47I GGWCC 2 cut(s) 203, 230
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 3 cut(s) 159, 189, 1065
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 702
FaeI CATG 4 cut(s) 151, 349, 824, 1040
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 2 cut(s) 216, 521
FatI CATG 4 cut(s) 147, 345, 820, 1036
FauI CCCGC 1 cut(s) 762
Fnu4HI GCNGC 6 cut(s) 330, 441, 878, 954, 959, 969
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 6 cut(s) 330, 441, 878, 954, 959, 969
FspBI CTAG 1 cut(s) 398
GlaI GCGC 2 cut(s) 561, 957
GluI GCNGC 6 cut(s) 330, 441, 878, 954, 959, 969
HaeIII GGCC 9 cut(s) 43, 99, 152, 164, 264, 273, 371, 410, 542
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 241
HhaI GCGC 2 cut(s) 562, 958
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 151, 349, 824, 1040
Hin6I GCGC 2 cut(s) 560, 956
HinP1I GCGC 2 cut(s) 560, 956
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 9 cut(s) 77, 116, 197, 324, 341, 509, 596, 635, 1020
HpaII CCGG 1 cut(s) 201
HphI GGTGA 5 cut(s) 116, 149, 302, 560, 958
Hpy166II GTNNAC 2 cut(s) 295, 753
Hpy188I TCNGA 7 cut(s) 76, 186, 730, 849, 862, 912, 997
Hpy188III TCNNGA 4 cut(s) 319, 696, 793, 1024
Hpy8I GTNNAC 2 cut(s) 295, 753
Hpy99I CGWCG 1 cut(s) 811
HpyAV CCTTC 3 cut(s) 110, 383, 1119
HpyCH4III ACNGT 3 cut(s) 807, 817, 1051
HpyCH4IV ACGT 4 cut(s) 492, 501, 585, 711
HpyCH4V TGCA 4 cut(s) 616, 820, 857, 961
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 377, 456, 613
HpyF3I CTNAG 6 cut(s) 276, 571, 576, 881, 1082, 1146
HpySE526I ACGT 4 cut(s) 492, 501, 585, 711
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 151, 349, 824, 1040
HspAI GCGC 2 cut(s) 560, 956
Kzo9I GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
LmnI GCTCC 3 cut(s) 334, 772, 874
Lsp1109I GCAGC 6 cut(s) 341, 452, 889, 940, 945, 980
LweI GCATC 1 cut(s) 866
MaeI CTAG 1 cut(s) 398
MaeII ACGT 4 cut(s) 492, 501, 585, 711
MalI GATC 7 cut(s) 183, 300, 464, 700, 825, 840, 999
MboI GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
MboII GAAGA 7 cut(s) 7, 39, 42, 213, 216, 398, 1054
MlsI TGGCCA 1 cut(s) 410
MluNI TGGCCA 1 cut(s) 410
MlyI GAGTC 5 cut(s) 110, 503, 605, 629, 1029
MmeI TCCRAC 1 cut(s) 853
Mox20I TGGCCA 1 cut(s) 410
MroXI GAANNNNTTC 2 cut(s) 393, 906
MscI TGGCCA 1 cut(s) 410
MseI TTAA 6 cut(s) 555, 653, 675, 720, 1101, 1156
MslI CAYNNNNRTG 4 cut(s) 350, 630, 1035, 1049
Msp20I TGGCCA 1 cut(s) 410
MspA1I CMGCKG 3 cut(s) 332, 443, 575
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 3 cut(s) 161, 191, 1067
MvaI CCWGG 3 cut(s) 161, 191, 1067
MwoI GCNNNNNNNGC 4 cut(s) 270, 377, 456, 613
NcoI CCATGG 1 cut(s) 147
NdeII GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
NlaIII CATG 4 cut(s) 151, 349, 824, 1040
NlaIV GGNNCC 5 cut(s) 232, 241, 744, 774, 804
PaeR7I CTCGAG 1 cut(s) 176
PceI AGGCCT 1 cut(s) 371
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdmI GAANNNNTTC 2 cut(s) 393, 906
PfeI GAWTC 4 cut(s) 77, 197, 324, 341
PflMI CCANNNNNTGG 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 1065
PkrI GCNGC 6 cut(s) 331, 442, 879, 955, 960, 970
PleI GAGTC 5 cut(s) 110, 503, 604, 629, 1028
PpsI GAGTC 5 cut(s) 110, 503, 604, 629, 1028
Ppu21I YACGTR 1 cut(s) 493
Psp1406I AACGTT 1 cut(s) 501
Psp6I CCWGG 3 cut(s) 159, 189, 1065
PspGI CCWGG 3 cut(s) 159, 189, 1065
PspN4I GGNNCC 5 cut(s) 232, 241, 744, 774, 804
PspPI GGNCC 4 cut(s) 42, 203, 230, 272
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 1 cut(s) 963
PstNI CAGNNNCTG 1 cut(s) 847
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 3 cut(s) 110, 752, 761
RsaNI GTAC 3 cut(s) 109, 751, 760
RseI CAYNNNNRTG 4 cut(s) 350, 630, 1035, 1049
SaqAI TTAA 6 cut(s) 555, 653, 675, 720, 1101, 1156
SatI GCNGC 6 cut(s) 330, 441, 878, 954, 959, 969
Sau3AI GATC 7 cut(s) 181, 298, 462, 698, 823, 838, 997
Sau96I GGNCC 4 cut(s) 42, 203, 230, 272
SchI GAGTC 5 cut(s) 110, 503, 605, 629, 1029
ScrFI CCNGG 3 cut(s) 161, 191, 1067
SfaNI GCATC 1 cut(s) 866
SfcI CTRYAG 1 cut(s) 959
SfiI GGCCNNNNNGGCC 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 2 cut(s) 203, 230
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 4 cut(s) 350, 630, 1035, 1049
SmlI CTYRAG 4 cut(s) 176, 317, 719, 793
SmoI CTYRAG 4 cut(s) 176, 317, 719, 793
SnaBI TACGTA 1 cut(s) 493
SseBI AGGCCT 1 cut(s) 371
SsiI CCGC 4 cut(s) 332, 443, 518, 769
SspMI CTAG 1 cut(s) 398
StuI AGGCCT 1 cut(s) 371
StyD4I CCNGG 3 cut(s) 159, 189, 1065
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 3 cut(s) 807, 817, 1051
TaiI ACGT 4 cut(s) 495, 504, 588, 714
TaqI TCGA 3 cut(s) 177, 195, 809
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 4 cut(s) 77, 197, 324, 341
Tru1I TTAA 6 cut(s) 555, 653, 675, 720, 1101, 1156
Tru9I TTAA 6 cut(s) 555, 653, 675, 720, 1101, 1156
TscAI CASTG 3 cut(s) 422, 429, 1054
TseI GCWGC 6 cut(s) 329, 440, 877, 953, 958, 968
TspDTI ATGAA 1 cut(s) 794
TspGWI ACGGA 3 cut(s) 282, 879, 1112
TspRI CASTG 3 cut(s) 422, 429, 1054
Van91I CCANNNNNTGG 1 cut(s) 417
Vha464I CTTAAG 1 cut(s) 719
VpaK11BI GGWCC 2 cut(s) 203, 230
XagI CCTNNNNNAGG 1 cut(s) 176
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 2 cut(s) 393, 906
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.