pycom15g21180

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002066
Physical Location & Seq
Reverse (-)
15181466 .. 15183175
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21180.1

Sequence Viewer

Length: 843 bp
ATGTCAGTAGACGTGATACGTAAAGCATATCAAGCAACAGAAGAGGGATTCCTCTCTGTTGTTACCAAACAATGGCCTATGAAACCCCAAATTGCAGCTGTTGGATCTTGTTGCCTTGCTGGTGTGATTTGCGGTGGCACCCTTTACATTGCCAACGTTGGTGACTCCCGTGCTGTGCTGGGGAGAGTTATGAAGGCTACAGGGGAGGTTCGTGCCATCCAGTTGTCATCAGAGCACAATGTGGCCATAGAATCTGTCCGACAGGAGATGCATTCTTTACACCCTGATGACTCACATATTGTAGTTTTAAAGCATAACGTATGGCGCGTGAAGGGCTTGATACAGGTTTCTAGATCTATTGGTGATGTATATCTTAAAAAGGCTGAATTTAACAGAGCGCCTTTGTATACTAAGTTTCGGTTACGAGAACCTTTTAAAAGGCCAATTTTAAGCTCTGAACCATCGATCTCTGTGCATGAACTTCAACCACATGATCAGTTTCTCATACTTGCTTCCGATGGGCTGTGGGAGCACCTTACCAACCAGGAGGCAGTTGATATTGTTCAAAATCACCCACGCAGCGGAAGTGCTCGGAGGCTTGTGAAAACTGCCTTGCAGGAAGCAGCTAAGAAAAGAGAAATGAGATACTCAGACTTGAAGAAAATAGAGCGTGGTGTCCGCCGGCATTTCCACGACGACATAACAGTCATAGTTTTATTCCTTGACTCGAACCTTGTGAGCAGAGCCAGCTCAATGAGATGCCCTACTTTGTCCGTGAGAGGAACTGGTGTTCATATACCATCAAAAACTCTTGCCCCATGTTCCACACCCATGGATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

281

Amino Acids

31.28

Weight (kDa)

9.51

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 8 - 209 7.4e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 72
AccI GTMKAC 2 cut(s) 9, 407
AccII CGCG 1 cut(s) 327
AciI CCGC 3 cut(s) 132, 582, 679
AclI AACGTT 1 cut(s) 156
AclWI GGATC 1 cut(s) 112
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 386
AdeI CACNNNGTG 1 cut(s) 241
AfiI CCNNNNNNNGG 2 cut(s) 72, 581
AgsI TTSAA 3 cut(s) 485, 566, 658
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 1 cut(s) 543
AloI GAACNNNNNNTCC 2 cut(s) 774, 806
AluBI AGCT 4 cut(s) 98, 453, 626, 750
AluI AGCT 4 cut(s) 98, 453, 626, 750
Alw21I GWGCWC 3 cut(s) 237, 534, 592
AlwI GGATC 1 cut(s) 112
AoxI GGCC 3 cut(s) 74, 243, 440
ApeKI GCWGC 3 cut(s) 95, 579, 623
ApoI RAATTY 1 cut(s) 386
AspLEI GCGC 2 cut(s) 327, 400
AsuHPI GGTGA 3 cut(s) 173, 374, 563
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 1 cut(s) 137
Bbv12I GWGCWC 3 cut(s) 237, 534, 592
BbvI GCAGC 3 cut(s) 107, 591, 635
BccI CCATC 4 cut(s) 224, 469, 512, 808
BcgI CGANNNNNNTGC 2 cut(s) 454, 488
BciT130I CCWGG 1 cut(s) 545
BciVI GTATCC 1 cut(s) 829
BclI TGATCA 1 cut(s) 493
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 401
BfuI GTATCC 1 cut(s) 829
BglII AGATCT 1 cut(s) 353
BisI GCNGC 3 cut(s) 96, 580, 624
BlsI GCNGC 3 cut(s) 97, 581, 625
Bme1390I CCNGG 1 cut(s) 545
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 545
BmsI GCATC 2 cut(s) 258, 749
Bsa29I ATCGAT 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 20
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 831
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 581
Bse118I RCCGGY 1 cut(s) 681
Bse1I ACTGG 2 cut(s) 220, 790
Bse3DI GCAATG 1 cut(s) 147
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 545
BseCI ATCGAT 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 1 cut(s) 216
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 72, 581
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 2 cut(s) 220, 790
BseXI GCAGC 3 cut(s) 107, 591, 635
BseYI CCCAGC 1 cut(s) 178
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 3 cut(s) 76, 245, 442
BshNI GGYRCC 1 cut(s) 137
BshVI ATCGAT 1 cut(s) 464
BsiHKAI GWGCWC 3 cut(s) 237, 534, 592
BsiSI CCGG 1 cut(s) 682
BslI CCNNNNNNNGG 2 cut(s) 72, 581
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 3 cut(s) 76, 245, 442
Bsp1286I GDGCHC 3 cut(s) 237, 534, 592
Bsp143I GATC 4 cut(s) 104, 353, 465, 493
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 3 cut(s) 132, 582, 679
BspANI GGCC 3 cut(s) 76, 245, 442
BspCNI CTCAG 1 cut(s) 662
BspDI ATCGAT 1 cut(s) 464
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 1 cut(s) 139
BspPI GGATC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 681
BsrI ACTGG 2 cut(s) 220, 790
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 4 cut(s) 104, 353, 465, 493
BssNAI GTATAC 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 831
Bst1107I GTATAC 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 1 cut(s) 36
BstBAI YACGTR 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 683, 748
BstDEI CTNAG 3 cut(s) 411, 627, 649
BstDSI CCRYGG 1 cut(s) 831
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 327, 400
BstKTI GATC 4 cut(s) 107, 356, 468, 496
BstMBI GATC 4 cut(s) 104, 353, 465, 493
BstMWI GCNNNNNNNGC 4 cut(s) 32, 333, 529, 747
BstNI CCWGG 1 cut(s) 545
BstSCI CCNGG 1 cut(s) 543
BstSFI CTRYAG 1 cut(s) 198
BstSNI TACGTA 1 cut(s) 20
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 3 cut(s) 107, 591, 635
BstX2I RGATCY 2 cut(s) 104, 353
BstXI CCANNNNNNTGG 1 cut(s) 832
BstYI RGATCY 2 cut(s) 104, 353
BstZ17I GTATAC 1 cut(s) 408
Bsu15I ATCGAT 1 cut(s) 464
BsuI GTATCC 1 cut(s) 829
BsuRI GGCC 3 cut(s) 76, 245, 442
BsuTUI ATCGAT 1 cut(s) 464
BtgI CCRYGG 1 cut(s) 831
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 683, 748
CfoI GCGC 2 cut(s) 327, 400
Cfr10I RCCGGY 1 cut(s) 681
ClaI ATCGAT 1 cut(s) 464
CviAII CATG 4 cut(s) 476, 491, 819, 832
DdeI CTNAG 3 cut(s) 411, 627, 649
DpnI GATC 4 cut(s) 106, 355, 467, 495
DpnII GATC 4 cut(s) 104, 353, 465, 493
DraI TTTAAA 2 cut(s) 309, 436
DraIII CACNNNGTG 1 cut(s) 241
DrdI GACNNNNNNGTC 1 cut(s) 704
DseDI GACNNNNNNGTC 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 1 cut(s) 668
Eco105I TACGTA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 831
EcoRII CCWGG 1 cut(s) 543
EcoT14I CCWWGG 1 cut(s) 831
EcoT22I ATGCAT 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 831
FaeI CATG 4 cut(s) 479, 494, 822, 835
FatI CATG 4 cut(s) 475, 490, 818, 831
FbaI TGATCA 1 cut(s) 493
FblI GTMKAC 2 cut(s) 9, 407
Fnu4HI GCNGC 3 cut(s) 96, 580, 624
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 3 cut(s) 96, 580, 624
FspBI CTAG 1 cut(s) 351
GlaI GCGC 2 cut(s) 326, 399
GluI GCNGC 3 cut(s) 96, 580, 624
GsaI CCCAGC 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 3 cut(s) 76, 245, 442
HapII CCGG 1 cut(s) 682
HhaI GCGC 2 cut(s) 327, 400
Hin1II CATG 4 cut(s) 479, 494, 822, 835
Hin6I GCGC 2 cut(s) 325, 398
HinP1I GCGC 2 cut(s) 325, 398
HinfI GANTC 5 cut(s) 48, 164, 251, 290, 725
HpaII CCGG 1 cut(s) 682
HphI GGTGA 3 cut(s) 173, 374, 563
Hpy166II GTNNAC 2 cut(s) 10, 408
Hpy188I TCNGA 6 cut(s) 232, 260, 457, 517, 594, 652
Hpy188III TCNNGA 1 cut(s) 351
Hpy8I GTNNAC 2 cut(s) 10, 408
Hpy99I CGWCG 1 cut(s) 698
HpyAV CCTTC 2 cut(s) 187, 325
HpyCH4III ACNGT 1 cut(s) 706
HpyCH4IV ACGT 4 cut(s) 12, 19, 156, 318
HpyCH4V TGCA 4 cut(s) 95, 271, 475, 616
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 333, 529, 747
HpyF3I CTNAG 3 cut(s) 411, 627, 649
HpySE526I ACGT 4 cut(s) 12, 19, 156, 318
Hsp92II CATG 4 cut(s) 479, 494, 822, 835
HspAI GCGC 2 cut(s) 325, 398
KroI GCCGGC 1 cut(s) 681
KroNI GCCGGC 1 cut(s) 683
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 4 cut(s) 104, 353, 465, 493
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 3 cut(s) 107, 591, 635
LweI GCATC 2 cut(s) 258, 749
MaeI CTAG 1 cut(s) 351
MaeII ACGT 4 cut(s) 12, 19, 156, 318
MaeIII GTNAC 3 cut(s) 61, 161, 420
MalI GATC 4 cut(s) 106, 355, 467, 495
MboI GATC 4 cut(s) 104, 353, 465, 493
MboII GAAGA 2 cut(s) 53, 670
MflI RGATCY 2 cut(s) 104, 353
MhlI GDGCHC 3 cut(s) 237, 534, 592
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 3 cut(s) 90, 386, 444
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 3 cut(s) 158, 284, 719
MmeI TCCRAC 2 cut(s) 82, 283
MnlI CCTC 6 cut(s) 37, 62, 199, 541, 588, 773
Mox20I TGGCCA 1 cut(s) 245
Mph1103I ATGCAT 1 cut(s) 273
MroNI GCCGGC 1 cut(s) 681
MscI TGGCCA 1 cut(s) 245
MseI TTAA 5 cut(s) 308, 375, 390, 435, 449
MslI CAYNNNNRTG 2 cut(s) 285, 830
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 98, 582
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 1 cut(s) 545
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 1 cut(s) 545
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 4 cut(s) 32, 333, 529, 747
NaeI GCCGGC 1 cut(s) 683
NcoI CCATGG 1 cut(s) 831
NdeII GATC 4 cut(s) 104, 353, 465, 493
NgoMIV GCCGGC 1 cut(s) 681
NlaIII CATG 4 cut(s) 479, 494, 822, 835
NlaIV GGNNCC 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 1 cut(s) 271
PdiI GCCGGC 1 cut(s) 683
PfeI GAWTC 2 cut(s) 48, 251
PflMI CCANNNNNTGG 1 cut(s) 72
PkrI GCNGC 3 cut(s) 97, 581, 625
PleI GAGTC 3 cut(s) 158, 284, 719
PpsI GAGTC 3 cut(s) 158, 284, 719
Ppu21I YACGTR 1 cut(s) 20
Psp1406I AACGTT 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 178
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 1 cut(s) 139
PsuI RGATCY 2 cut(s) 104, 353
PvuII CAGCTG 1 cut(s) 98
RseI CAYNNNNRTG 2 cut(s) 285, 830
SaqAI TTAA 5 cut(s) 308, 375, 390, 435, 449
SatI GCNGC 3 cut(s) 96, 580, 624
Sau3AI GATC 4 cut(s) 104, 353, 465, 493
SchI GAGTC 3 cut(s) 158, 284, 719
ScrFI CCNGG 1 cut(s) 545
SduI GDGCHC 3 cut(s) 237, 534, 592
SfaNI GCATC 2 cut(s) 258, 749
SfcI CTRYAG 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 285, 830
SnaBI TACGTA 1 cut(s) 20
Sse9I AATT 3 cut(s) 90, 386, 444
SsiI CCGC 3 cut(s) 132, 582, 679
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 543
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 1 cut(s) 706
TaiI ACGT 4 cut(s) 15, 22, 159, 321
TaqI TCGA 2 cut(s) 464, 728
TasI AATT 3 cut(s) 90, 386, 444
TfiI GAWTC 2 cut(s) 48, 251
Tru1I TTAA 5 cut(s) 308, 375, 390, 435, 449
Tru9I TTAA 5 cut(s) 308, 375, 390, 435, 449
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 3 cut(s) 95, 579, 623
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 4 cut(s) 95, 206, 492, 782
TspGWI ACGGA 1 cut(s) 763
Van91I CCANNNNNTGG 1 cut(s) 72
XapI RAATTY 1 cut(s) 386
XbaI TCTAGA 1 cut(s) 350
XmiI GTMKAC 2 cut(s) 9, 407
XspI CTAG 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.