Rroxscaffold_7G00189930

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002066
Physical Location & Seq
Reverse (-)
29745538 .. 29750683
5146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189930.1

Sequence Viewer

Length: 1245 bp
ATGTTATCAGAGTTGATGAACTTCTTGAGGTCCTGTTTTCGGCCGAGGTCGAATGGATACGTTCATACCGGTTCAGATGCAGGGGGTCGCCAAGACGGGCTCCTGTGGTACAAAGACACAGGCCAACACATCAATGGTGATTTCTCAATGGCTGTAGTTCAGGCCAACAATTTGCTTGAGGATCAGAGTCAGGTTGAGTCCGGGAGTTTGAGCACGAGTGAGTCTGGCCCTTTTGGAACTTTTATTGGTGTTTATGACGGGCATGGTGGACCGGAGACATCACGCTTTATCAATGATCACCTCTTTCAGCATCTCAAGAGATTTAGTTCGGAGCAGCAGTCCATGTCTGTGGATGTAATACGAAAAGCCTTTCAAGCAACCGAAGAGGGTTTTCTTTCTGTAGTTACCAGGCAGTGGCCTCTGAAACCACAAATTGCAGCAGTAGGATCCTGCTGCCTTGTTGGCGTTATTTGTAACGGAACTCTTTATATTGCCAACCTTGGTGATTCCCGTGCTGTTTTGGGGAGGATTGTGAAGGCAACAGGGGAGGTACTCTCAATTCAGTTGTCACAAGAGCACAATGCATGTATAGAATCTGTGAGACAGGAGCTGCATTCTTTGCACCCCGACGATCCACAAATTGTACTTTTGAAGCATAATGTTTGGCGTGTAAAGGGAGTAATACAGATTTCTAGATCTATTGGTGACTTCTATTTAAAGAAGGCTGAATTTAACAGGGAGCCTTTATATGCCAAGTTTCGTCTTCGGGACCCTATCAAAAGGCCAATATTGAGCTCTGACCCCGCAATTTCAGTGCTCCAATTGCAACCACACGATCAATTTATTATATTTGCTTCTGATGGGCTCTGGGAGCATCTCAGCAATCAGGAAGCAGTTGATATAGTTCAAAATCATCCCCGAAATGGAAGTGCAAAGCGGTTGGTGAAAACTGCCCTGCAAGAAGCAGCTAAAAAGAGAGAAATGCGATATTCAGACTTGAAGAAGATTGACCGTGGTGTTCGTCGCCATTTCCACGATGACATAACCGTAATTGTTGTGTTTCTCGACTCAAATCTTGTGAGCAAGGCTACTTCTGTCAAGTGCTCAGTGAGAGGGCGGTATCAGCCTGCCTCCGAATACTCTGGCTCCTTACACGACTACACCCACGGAAGCTGGCAATTCCTGATCATTACAAACTGTGTCTGGTTGTGTGTTTGTACCATTCTCTTGTGTGAATACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

414

Amino Acids

46.52

Weight (kDa)

8.01

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 80 - 323 2.7e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 414
AciI CCGC 3 cut(s) 804, 937, 1117
AclWI GGATC 4 cut(s) 189, 441, 454, 626
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 728
AfaI GTAC 4 cut(s) 110, 552, 645, 1219
AfiI CCNNNNNNNGG 3 cut(s) 39, 414, 923
AgeI ACCGGT 1 cut(s) 68
AgsI TTSAA 4 cut(s) 374, 652, 908, 1000
AjnI CCWGG 1 cut(s) 407
AluBI AGCT 4 cut(s) 610, 795, 968, 1173
AluI AGCT 4 cut(s) 610, 795, 968, 1173
Alw21I GWGCWC 5 cut(s) 215, 579, 797, 819, 1106
Alw26I GTCTC 2 cut(s) 269, 595
AlwI GGATC 4 cut(s) 189, 441, 454, 626
AlwNI CAGNNNCTG 1 cut(s) 610
AoxI GGCC 6 cut(s) 41, 121, 162, 226, 416, 782
ApeKI GCWGC 5 cut(s) 334, 437, 453, 610, 965
ApoI RAATTY 1 cut(s) 728
AsiGI ACCGGT 1 cut(s) 68
AspS9I GGNCC 4 cut(s) 30, 227, 269, 769
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 5 cut(s) 149, 290, 515, 716, 955
AvaII GGWCC 3 cut(s) 30, 269, 769
BamHI GGATCC 1 cut(s) 446
BanII GRGCYC 3 cut(s) 102, 797, 867
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 5 cut(s) 215, 579, 797, 819, 1106
BbvI GCAGC 5 cut(s) 346, 440, 449, 597, 977
BccI CCATC 1 cut(s) 854
BciT130I CCWGG 1 cut(s) 409
BciVI GTATCC 1 cut(s) 50
BclI TGATCA 2 cut(s) 295, 1185
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 269, 595
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 2 cut(s) 153, 399
BfuI GTATCC 1 cut(s) 50
BglI GCCNNNNNGGC 1 cut(s) 462
BglII AGATCT 1 cut(s) 695
BisI GCNGC 5 cut(s) 335, 438, 454, 611, 966
BlsI GCNGC 5 cut(s) 336, 439, 455, 612, 967
Bme1390I CCNGG 2 cut(s) 202, 409
Bme18I GGWCC 3 cut(s) 30, 269, 769
BmgT120I GGNCC 4 cut(s) 30, 227, 269, 769
BmiI GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1147
BmrFI CCNGG 2 cut(s) 202, 409
BmsI GCATC 3 cut(s) 67, 319, 883
BpiI GAAGAC 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpuEI CTTGAG 3 cut(s) 46, 197, 299
BpuMI CCSGG 1 cut(s) 202
BsaJI CCNNGG 4 cut(s) 44, 499, 1012, 1165
BsaWI WCCGGW 2 cut(s) 68, 271
BsaXI ACNNNNNCTCC 4 cut(s) 323, 353, 1130, 1160
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 414, 923
Bse118I RCCGGY 1 cut(s) 68
BseBI CCWGG 1 cut(s) 409
BseDI CCNNGG 4 cut(s) 44, 499, 1012, 1165
BseGI GGATG 2 cut(s) 358, 913
BseLI CCNNNNNNNGG 3 cut(s) 39, 414, 923
BseMII CTCAG 2 cut(s) 892, 1119
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 5 cut(s) 346, 440, 449, 597, 977
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 6 cut(s) 43, 123, 164, 228, 418, 784
BshTI ACCGGT 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 5 cut(s) 215, 579, 797, 819, 1106
BsiSI CCGG 3 cut(s) 69, 201, 272
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 3 cut(s) 39, 414, 923
BsmAI GTCTC 2 cut(s) 269, 595
BsmFI GGGAC 1 cut(s) 782
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 6 cut(s) 43, 123, 164, 228, 418, 784
Bsp1286I GDGCHC 7 cut(s) 102, 215, 579, 797, 819, 867, 1106
Bsp143I GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
BspACI CCGC 3 cut(s) 804, 937, 1117
BspANI GGCC 6 cut(s) 43, 123, 164, 228, 418, 784
BspCNI CTCAG 2 cut(s) 891, 1118
BspLI GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1147
BspPI GGATC 4 cut(s) 189, 441, 454, 626
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 4 cut(s) 44, 499, 1012, 1165
BssMI GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 409
Bst4CI ACNGT 3 cut(s) 1013, 1048, 1199
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 1128, 1175
BstDEI CTNAG 2 cut(s) 878, 1105
BstDSI CCRYGG 2 cut(s) 1012, 1165
BstF5I GGATG 2 cut(s) 358, 913
BstKTI GATC 7 cut(s) 184, 298, 449, 634, 698, 838, 1188
BstMAI GTCTC 2 cut(s) 269, 595
BstMBI GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 6 cut(s) 374, 462, 619, 823, 871, 1123
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 2 cut(s) 200, 407
BstSFI CTRYAG 2 cut(s) 153, 399
BstV1I GCAGC 5 cut(s) 346, 440, 449, 597, 977
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 2 cut(s) 446, 695
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 2 cut(s) 446, 695
BstZI CGGCCG 1 cut(s) 41
BsuI GTATCC 1 cut(s) 50
BsuRI GGCC 6 cut(s) 43, 123, 164, 228, 418, 784
BtgI CCRYGG 2 cut(s) 1012, 1165
BtsCI GGATG 2 cut(s) 358, 913
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 3 cut(s) 419, 819, 1113
Cac8I GCNNGC 2 cut(s) 1128, 1175
CaiI CAGNNNCTG 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 4 cut(s) 30, 227, 269, 769
Csp6I GTAC 4 cut(s) 109, 551, 644, 1218
CspAI ACCGGT 1 cut(s) 68
CviAII CATG 3 cut(s) 263, 343, 585
CviQI GTAC 4 cut(s) 109, 551, 644, 1218
DdeI CTNAG 2 cut(s) 878, 1105
DpnI GATC 7 cut(s) 183, 297, 448, 633, 697, 837, 1187
DpnII GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Ecl136II GAGCTC 1 cut(s) 795
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 3 cut(s) 102, 797, 867
Eco47I GGWCC 3 cut(s) 30, 269, 769
Eco52I CGGCCG 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 795
EcoO109I RGGNCCY 2 cut(s) 30, 769
EcoRII CCWGG 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 3 cut(s) 102, 797, 867
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 3 cut(s) 266, 346, 588
FaqI GGGAC 1 cut(s) 782
FatI CATG 3 cut(s) 262, 342, 584
FauI CCCGC 1 cut(s) 811
FbaI TGATCA 2 cut(s) 295, 1185
Fnu4HI GCNGC 5 cut(s) 335, 438, 454, 611, 966
FokI GGATG 2 cut(s) 365, 900
FriOI GRGCYC 3 cut(s) 102, 797, 867
Fsp4HI GCNGC 5 cut(s) 335, 438, 454, 611, 966
FspBI CTAG 1 cut(s) 693
GluI GCNGC 5 cut(s) 335, 438, 454, 611, 966
HaeIII GGCC 6 cut(s) 43, 123, 164, 228, 418, 784
HapII CCGG 3 cut(s) 69, 201, 272
Hin1II CATG 3 cut(s) 266, 346, 588
HinfI GANTC 6 cut(s) 187, 197, 221, 506, 593, 1067
HpaII CCGG 3 cut(s) 69, 201, 272
HphI GGTGA 5 cut(s) 149, 290, 515, 716, 955
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 9 cut(s) 10, 76, 186, 331, 423, 799, 859, 994, 1135
Hpy188III TCNNGA 7 cut(s) 25, 316, 693, 767, 887, 1064, 1183
Hpy8I GTNNAC 1 cut(s) 269
Hpy99I CGWCG 2 cut(s) 632, 1026
HpyAV CCTTC 2 cut(s) 529, 715
HpyCH4III ACNGT 3 cut(s) 1013, 1048, 1199
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 8 cut(s) 80, 437, 584, 613, 622, 826, 932, 958
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 462, 619, 823, 871, 1123
HpyF3I CTNAG 2 cut(s) 878, 1105
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 266, 346, 588
KflI GGGWCCC 1 cut(s) 769
Ksp22I TGATCA 2 cut(s) 295, 1185
Kzo9I GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
LmnI GCTCC 7 cut(s) 105, 331, 607, 739, 822, 871, 1151
Lsp1109I GCAGC 5 cut(s) 346, 440, 449, 597, 977
LweI GCATC 3 cut(s) 67, 319, 883
MaeI CTAG 1 cut(s) 693
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 4 cut(s) 403, 473, 567, 704
MalI GATC 7 cut(s) 183, 297, 448, 633, 697, 837, 1187
MboI GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
MboII GAAGA 4 cut(s) 395, 755, 1012, 1015
MfeI CAATTG 1 cut(s) 821
MflI RGATCY 2 cut(s) 446, 695
MhlI GDGCHC 7 cut(s) 102, 215, 579, 797, 819, 867, 1106
MlyI GAGTC 4 cut(s) 196, 206, 230, 1061
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 2 cut(s) 716, 732
MslI CAYNNNNRTG 2 cut(s) 132, 347
MspI CCGG 3 cut(s) 69, 201, 272
MspR9I CCNGG 2 cut(s) 202, 409
MunI CAATTG 1 cut(s) 821
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 1 cut(s) 409
MwoI GCNNNNNNNGC 6 cut(s) 374, 462, 619, 823, 871, 1123
NciI CCSGG 1 cut(s) 202
NdeII GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
NlaIII CATG 3 cut(s) 266, 346, 588
NlaIV GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1147
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 567, 704
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 1 cut(s) 588
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 506, 593
PflMI CCANNNNNTGG 1 cut(s) 414
PfoI TCCNGGA 1 cut(s) 200
PinAI ACCGGT 1 cut(s) 68
PkrI GCNGC 5 cut(s) 336, 439, 455, 612, 967
PleI GAGTC 4 cut(s) 195, 205, 229, 1061
PpsI GAGTC 4 cut(s) 195, 205, 229, 1061
PpuMI RGGWCCY 2 cut(s) 30, 769
Psp124BI GAGCTC 1 cut(s) 797
Psp5II RGGWCCY 2 cut(s) 30, 769
Psp6I CCWGG 1 cut(s) 407
PspGI CCWGG 1 cut(s) 407
PspN4I GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1147
PspPI GGNCC 4 cut(s) 30, 227, 269, 769
PspPPI RGGWCCY 2 cut(s) 30, 769
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 2 cut(s) 446, 695
RsaI GTAC 4 cut(s) 110, 552, 645, 1219
RsaNI GTAC 4 cut(s) 109, 551, 644, 1218
RseI CAYNNNNRTG 2 cut(s) 132, 347
SacI GAGCTC 1 cut(s) 797
SaqAI TTAA 2 cut(s) 716, 732
SatI GCNGC 5 cut(s) 335, 438, 454, 611, 966
Sau3AI GATC 7 cut(s) 181, 295, 446, 631, 695, 835, 1185
Sau96I GGNCC 4 cut(s) 30, 227, 269, 769
SchI GAGTC 4 cut(s) 196, 206, 230, 1061
ScrFI CCNGG 2 cut(s) 202, 409
SduI GDGCHC 7 cut(s) 102, 215, 579, 797, 819, 867, 1106
SfaNI GCATC 3 cut(s) 67, 319, 883
SfcI CTRYAG 2 cut(s) 153, 399
SinI GGWCC 3 cut(s) 30, 269, 769
SmiMI CAYNNNNRTG 2 cut(s) 132, 347
SmlI CTYRAG 3 cut(s) 25, 176, 314
SmoI CTYRAG 3 cut(s) 25, 176, 314
SsiI CCGC 3 cut(s) 804, 937, 1117
SspI AATATT 1 cut(s) 789
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 797
StyD4I CCNGG 2 cut(s) 200, 407
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 3 cut(s) 1013, 1048, 1199
TaiI ACGT 1 cut(s) 63
TaqI TCGA 2 cut(s) 50, 1065
TatI WGTACW 1 cut(s) 643
TfiI GAWTC 2 cut(s) 506, 593
Tru1I TTAA 2 cut(s) 716, 732
Tru9I TTAA 2 cut(s) 716, 732
TscAI CASTG 3 cut(s) 419, 819, 1113
TseFI GTSAC 2 cut(s) 567, 704
TseI GCWGC 5 cut(s) 334, 437, 453, 610, 965
Tsp45I GTSAC 2 cut(s) 567, 704
TspDTI ATGAA 2 cut(s) 32, 53
TspGWI ACGGA 2 cut(s) 492, 1182
TspRI CASTG 3 cut(s) 419, 819, 1113
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 3 cut(s) 30, 269, 769
XapI RAATTY 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 1 cut(s) 588
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.