Rh6BG233000

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002066
Physical Location & Seq
Forward (+)
43903864 .. 43907466
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG233000.1

Sequence Viewer

Length: 1188 bp
ATGTTATCAGAGTTGATGAACTTCTTGAGGTCCTGTTTTCGGCCGAGGTCGAATGGATATGTTCATACCAGTTCAGATGCAGGGGGTCGCCAAGATGGGCTCCTGTGGTACAAAGACACAGGCCAGCACATCAATGGTGATTTCTCAATGGCTGTAGTTCAGGCCAACAATTTGCTTGAGGATCAGAGTCAGGTTGAGTCCGGGAGTTTGAGCACGAGTGAGTCTGGCCCTTTTGGAACTTTTATTGGTGTTTATGACGGGCATGGTGGACCGGAGACATCACGCTTTATCAATGATCACCTCTTTCAGCATCTAAAGAGATTTAGTTCGGAGCAGCAGTCCATGTCTGTGGATGTAATACGAAAAGCCTTTCAAGCAACCGAAGAGGGTTTTCTTTCTGTAGTTACCAGGCAGTGGCCTCTGAAACCACAAATTGCAGCAGTAGGATCCTGCTGCCTTGTTGGCGTTATTTGTAACGGAACTCTTTATATTGCCAACCTTGGTGATTCCCGTGCTGTTTTGGGGAGGATTGTGAAGGCAACAGGGGAGGTACTCTCAATTCAGTTGTCACAAGAGCACAATGCATGTATAGAATCTGTGAGACAGGAGCTGCATTCTTTGCACCCCGACGATCCACAGATTGTACTTTTGAAGCATAATGTTTGGCGTGTAAAGGGAGTAATACAGATTTCTAGATCTATTGGTGACTTCTATTTAAAGAAGGCTGAATTTAACAGGGAGCCTTTATATGCCAAGTTCCGTCTTCGGGACCCTATCAAAAGACCAATATTGAGCTCTGAGCCCGCAATTTCAGTGCTCCAATTGCAACCACACGATCAATTTATTATATTTGCTTCTGATGGGCTCTGGGAGCACCTCAGCAATCAGGAAGCAGTTGATATAGTTCAAAATCATCCCCGAAATGGAAGTGCAAAGCGGTTGGTGAAAACTGCACTGCAAGAAGCAGCTAAAAAGAGAGAAATGCGGTATTCAGACTTGAAGAAGATTGACCGTGGTGTTCGTCGCCATTTCCACGATGACATAACAGTAATTGTTGTGTTTCTCGACTCAAATCTTGTGAGCAAGGCTACTTCTGTCAAGTGCTCAGTGAGAGGGGGCGGTATCAGCCTGCCTCCTAATACTCTGGCTCCTTACACGACTACACCCACGGAAGCTGGCAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

395

Amino Acids

43.91

Weight (kDa)

8.45

Isoelectric Point (pI)

46.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 80 - 323 6.9e-39 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 414
AciI CCGC 4 cut(s) 804, 937, 985, 1119
AclWI GGATC 4 cut(s) 189, 441, 454, 626
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 728
AfaI GTAC 3 cut(s) 110, 552, 645
AfiI CCNNNNNNNGG 4 cut(s) 39, 414, 766, 923
AgsI TTSAA 4 cut(s) 374, 652, 908, 1000
AjnI CCWGG 1 cut(s) 407
AluBI AGCT 4 cut(s) 610, 795, 968, 1175
AluI AGCT 4 cut(s) 610, 795, 968, 1175
Alw21I GWGCWC 6 cut(s) 215, 579, 797, 819, 876, 1106
Alw26I GTCTC 2 cut(s) 269, 595
AlwI GGATC 4 cut(s) 189, 441, 454, 626
AlwNI CAGNNNCTG 1 cut(s) 610
AoxI GGCC 5 cut(s) 41, 121, 162, 226, 416
ApeKI GCWGC 5 cut(s) 334, 437, 453, 610, 965
ApoI RAATTY 1 cut(s) 728
AspS9I GGNCC 4 cut(s) 30, 227, 269, 769
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 5 cut(s) 149, 290, 515, 716, 955
AvaII GGWCC 3 cut(s) 30, 269, 769
BamHI GGATCC 1 cut(s) 446
BanII GRGCYC 4 cut(s) 102, 797, 804, 867
BauI CACGAG 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 6 cut(s) 215, 579, 797, 819, 876, 1106
BbvCI CCTCAGC 1 cut(s) 878
BbvI GCAGC 5 cut(s) 346, 440, 449, 597, 977
BccI CCATC 2 cut(s) 89, 854
BciT130I CCWGG 1 cut(s) 409
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 269, 595
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 2 cut(s) 153, 399
BglI GCCNNNNNGGC 1 cut(s) 462
BglII AGATCT 1 cut(s) 695
BisI GCNGC 5 cut(s) 335, 438, 454, 611, 966
BlsI GCNGC 5 cut(s) 336, 439, 455, 612, 967
Bme1390I CCNGG 2 cut(s) 202, 409
Bme18I GGWCC 3 cut(s) 30, 269, 769
BmgT120I GGNCC 4 cut(s) 30, 227, 269, 769
BmiI GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1149
BmrFI CCNGG 2 cut(s) 202, 409
BmsI GCATC 2 cut(s) 67, 319
BpiI GAAGAC 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 539, 571
Bpu10I CCTNAGC 1 cut(s) 878
BpuEI CTTGAG 2 cut(s) 46, 197
BpuMI CCSGG 1 cut(s) 202
BsaJI CCNNGG 4 cut(s) 44, 499, 1012, 1167
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 4 cut(s) 323, 353, 1132, 1162
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 414, 766, 923
Bse1I ACTGG 1 cut(s) 69
BseBI CCWGG 1 cut(s) 409
BseDI CCNNGG 4 cut(s) 44, 499, 1012, 1167
BseGI GGATG 2 cut(s) 358, 913
BseLI CCNNNNNNNGG 4 cut(s) 39, 414, 766, 923
BseMII CTCAG 3 cut(s) 789, 892, 1119
BseNI ACTGG 1 cut(s) 69
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 5 cut(s) 346, 440, 449, 597, 977
BsgI GTGCAG 1 cut(s) 936
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 5 cut(s) 43, 123, 164, 228, 418
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 6 cut(s) 215, 579, 797, 819, 876, 1106
BsiSI CCGG 2 cut(s) 201, 272
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 4 cut(s) 39, 414, 766, 923
BsmAI GTCTC 2 cut(s) 269, 595
BsmFI GGGAC 1 cut(s) 782
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 5 cut(s) 43, 123, 164, 228, 418
Bsp1286I GDGCHC 9 cut(s) 102, 215, 579, 797, 804, 819, 867, 876, 1106
Bsp143I GATC 6 cut(s) 181, 295, 446, 631, 695, 835
BspACI CCGC 4 cut(s) 804, 937, 985, 1119
BspANI GGCC 5 cut(s) 43, 123, 164, 228, 418
BspCNI CTCAG 3 cut(s) 790, 891, 1118
BspLI GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1149
BspPI GGATC 4 cut(s) 189, 441, 454, 626
BsrI ACTGG 1 cut(s) 69
BssECI CCNNGG 4 cut(s) 44, 499, 1012, 1167
BssMI GATC 6 cut(s) 181, 295, 446, 631, 695, 835
BssSI CACGAG 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 1013, 1048
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 619
BstC8I GCNNGC 4 cut(s) 125, 804, 1130, 1177
BstDEI CTNAG 3 cut(s) 798, 878, 1105
BstDSI CCRYGG 2 cut(s) 1012, 1167
BstF5I GGATG 2 cut(s) 358, 913
BstKTI GATC 6 cut(s) 184, 298, 449, 634, 698, 838
BstMAI GTCTC 2 cut(s) 269, 595
BstMBI GATC 6 cut(s) 181, 295, 446, 631, 695, 835
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 6 cut(s) 374, 462, 619, 823, 871, 1125
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 2 cut(s) 200, 407
BstSFI CTRYAG 2 cut(s) 153, 399
BstV1I GCAGC 5 cut(s) 346, 440, 449, 597, 977
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 2 cut(s) 446, 695
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 2 cut(s) 446, 695
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 5 cut(s) 43, 123, 164, 228, 418
BtgI CCRYGG 2 cut(s) 1012, 1167
BtsCI GGATG 2 cut(s) 358, 913
BtsI GCAGTG 2 cut(s) 419, 953
BtsIMutI CAGTG 4 cut(s) 419, 819, 953, 1113
Cac8I GCNNGC 4 cut(s) 125, 804, 1130, 1177
CaiI CAGNNNCTG 1 cut(s) 610
Cfr13I GGNCC 4 cut(s) 30, 227, 269, 769
Csp6I GTAC 3 cut(s) 109, 551, 644
CviAII CATG 3 cut(s) 263, 343, 585
CviQI GTAC 3 cut(s) 109, 551, 644
DdeI CTNAG 3 cut(s) 798, 878, 1105
DpnI GATC 6 cut(s) 183, 297, 448, 633, 697, 837
DpnII GATC 6 cut(s) 181, 295, 446, 631, 695, 835
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Ecl136II GAGCTC 1 cut(s) 795
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 4 cut(s) 102, 797, 804, 867
Eco47I GGWCC 3 cut(s) 30, 269, 769
Eco52I CGGCCG 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 795
EcoO109I RGGNCCY 2 cut(s) 30, 769
EcoRII CCWGG 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 4 cut(s) 102, 797, 804, 867
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 3 cut(s) 266, 346, 588
FaqI GGGAC 1 cut(s) 782
FatI CATG 3 cut(s) 262, 342, 584
FauI CCCGC 1 cut(s) 811
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 335, 438, 454, 611, 966
FokI GGATG 2 cut(s) 365, 900
FriOI GRGCYC 4 cut(s) 102, 797, 804, 867
Fsp4HI GCNGC 5 cut(s) 335, 438, 454, 611, 966
FspBI CTAG 1 cut(s) 693
GluI GCNGC 5 cut(s) 335, 438, 454, 611, 966
HaeIII GGCC 5 cut(s) 43, 123, 164, 228, 418
HapII CCGG 2 cut(s) 201, 272
Hin1II CATG 3 cut(s) 266, 346, 588
HinfI GANTC 6 cut(s) 187, 197, 221, 506, 593, 1067
HpaII CCGG 2 cut(s) 201, 272
HphI GGTGA 5 cut(s) 149, 290, 515, 716, 955
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 8 cut(s) 10, 76, 186, 331, 423, 799, 859, 994
Hpy188III TCNNGA 5 cut(s) 25, 693, 767, 887, 1064
Hpy8I GTNNAC 1 cut(s) 269
Hpy99I CGWCG 2 cut(s) 632, 1026
HpyAV CCTTC 2 cut(s) 529, 715
HpyCH4III ACNGT 2 cut(s) 1013, 1048
HpyCH4V TGCA 9 cut(s) 80, 437, 584, 613, 622, 826, 932, 953, 958
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 462, 619, 823, 871, 1125
HpyF3I CTNAG 3 cut(s) 798, 878, 1105
Hsp92II CATG 3 cut(s) 266, 346, 588
KflI GGGWCCC 1 cut(s) 769
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 6 cut(s) 181, 295, 446, 631, 695, 835
LmnI GCTCC 7 cut(s) 105, 331, 607, 739, 822, 871, 1153
Lsp1109I GCAGC 5 cut(s) 346, 440, 449, 597, 977
LweI GCATC 2 cut(s) 67, 319
MaeI CTAG 1 cut(s) 693
MaeIII GTNAC 4 cut(s) 403, 473, 567, 704
MalI GATC 6 cut(s) 183, 297, 448, 633, 697, 837
MboI GATC 6 cut(s) 181, 295, 446, 631, 695, 835
MboII GAAGA 4 cut(s) 395, 755, 1012, 1015
MfeI CAATTG 1 cut(s) 821
MflI RGATCY 2 cut(s) 446, 695
MhlI GDGCHC 9 cut(s) 102, 215, 579, 797, 804, 819, 867, 876, 1106
MluCI AATT 8 cut(s) 169, 432, 558, 728, 807, 821, 839, 1050
MlyI GAGTC 4 cut(s) 196, 206, 230, 1061
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 2 cut(s) 716, 732
MslI CAYNNNNRTG 2 cut(s) 132, 347
MspI CCGG 2 cut(s) 201, 272
MspR9I CCNGG 2 cut(s) 202, 409
MunI CAATTG 1 cut(s) 821
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 1 cut(s) 409
MwoI GCNNNNNNNGC 6 cut(s) 374, 462, 619, 823, 871, 1125
NciI CCSGG 1 cut(s) 202
NdeII GATC 6 cut(s) 181, 295, 446, 631, 695, 835
NlaIII CATG 3 cut(s) 266, 346, 588
NlaIV GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1149
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 567, 704
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 1 cut(s) 588
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 506, 593
PflMI CCANNNNNTGG 1 cut(s) 414
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 5 cut(s) 336, 439, 455, 612, 967
PleI GAGTC 4 cut(s) 195, 205, 229, 1061
PpsI GAGTC 4 cut(s) 195, 205, 229, 1061
PpuMI RGGWCCY 2 cut(s) 30, 769
Psp124BI GAGCTC 1 cut(s) 797
Psp5II RGGWCCY 2 cut(s) 30, 769
Psp6I CCWGG 1 cut(s) 407
PspGI CCWGG 1 cut(s) 407
PspN4I GGNNCC 6 cut(s) 101, 448, 741, 770, 771, 1149
PspPI GGNCC 4 cut(s) 30, 227, 269, 769
PspPPI RGGWCCY 2 cut(s) 30, 769
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 2 cut(s) 446, 695
RsaI GTAC 3 cut(s) 110, 552, 645
RsaNI GTAC 3 cut(s) 109, 551, 644
RseI CAYNNNNRTG 2 cut(s) 132, 347
SacI GAGCTC 1 cut(s) 797
SaqAI TTAA 2 cut(s) 716, 732
SatI GCNGC 5 cut(s) 335, 438, 454, 611, 966
Sau3AI GATC 6 cut(s) 181, 295, 446, 631, 695, 835
Sau96I GGNCC 4 cut(s) 30, 227, 269, 769
SchI GAGTC 4 cut(s) 196, 206, 230, 1061
ScrFI CCNGG 2 cut(s) 202, 409
SduI GDGCHC 9 cut(s) 102, 215, 579, 797, 804, 819, 867, 876, 1106
SfaNI GCATC 2 cut(s) 67, 319
SfcI CTRYAG 2 cut(s) 153, 399
SinI GGWCC 3 cut(s) 30, 269, 769
SmiMI CAYNNNNRTG 2 cut(s) 132, 347
SmlI CTYRAG 2 cut(s) 25, 176
SmoI CTYRAG 2 cut(s) 25, 176
Sse9I AATT 8 cut(s) 169, 432, 558, 728, 807, 821, 839, 1050
SsiI CCGC 4 cut(s) 804, 937, 985, 1119
SspI AATATT 1 cut(s) 789
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 797
StyD4I CCNGG 2 cut(s) 200, 407
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 2 cut(s) 1013, 1048
TaqI TCGA 2 cut(s) 50, 1065
TasI AATT 8 cut(s) 169, 432, 558, 728, 807, 821, 839, 1050
TatI WGTACW 1 cut(s) 643
TfiI GAWTC 2 cut(s) 506, 593
Tru1I TTAA 2 cut(s) 716, 732
Tru9I TTAA 2 cut(s) 716, 732
TscAI CASTG 4 cut(s) 419, 819, 960, 1113
TseFI GTSAC 2 cut(s) 567, 704
TseI GCWGC 5 cut(s) 334, 437, 453, 610, 965
Tsp45I GTSAC 2 cut(s) 567, 704
TspDTI ATGAA 2 cut(s) 32, 53
TspGWI ACGGA 3 cut(s) 492, 749, 1184
TspRI CASTG 4 cut(s) 419, 819, 960, 1113
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 3 cut(s) 30, 269, 769
XapI RAATTY 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 1 cut(s) 588
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.