RLG00000016799

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002066
Physical Location & Seq
Reverse (-)
10092254 .. 10095230
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016799

Sequence Viewer

Length: 1269 bp
ATGGCTGTCACTATTTCAAGCAACTCTATACTGCTTGTGTTGCTTTCTTCAGTTCAGGATAGTATTGGCATCATGCGGGGGATAAGGCTGGTGGGGTTATTGGTTGGAGGAAGTATTGCATCCATTTTGGATGTGGAGGCATTTGAGGCTGGCCCAGGTTCTGATGCAGCTGGCAAACAAGAAGGATTACTTTGGTATAAAGACTTTGGGCAACACTTGAATGGTGACTTTTCAATGGCTGTTGTACAGGCCAACAATTTGCTTGAGGATCAGAGCCAGCTTGAGTCTGGTCCCTTGAGCTCGCTTGAGTCTGGCCCCAATGGCACTTTTATTGGAGTTTATGATGGGCACGGCGGCCCTGAGACCTCTCGTTATGTCAATGATCACCTATTCCAGAATTTGAAAAGGTTCACGTCAGAACAACAGTCCATGTCAGTGGATGTGATACGGAAAGCATATCAAGCAACAGAAGATGGATTCCTCTCTATTGTTACCAAACAATGGCCCGTGAAGCCCCAAATTGCAGCTGTTGGATCTTGTTGCCTTGTTGGTGTGATCTGTGGAGGCACACTTTACATTGCCAACGTTGGTGACTCCCGCGCTGTGTTGGGGAGAGTTGTGAAGGCAACTGGAGAGGTACTTGCCATCCAGCTGTCAGCAGAGCACAATGTGGCGATAGAGTCTGTCAGACGGGAGATGCATTCTTTGCATCCAGACGACTCACATATTGTAGTTTTGAAGCATAATGTATGGCGTGTAAAGGGATTGATACAGATTTCTAGATCCATTGGAGATGTATATCTAAAAAAGCCCGAGTTTAACAGGGAACCTTTGTATGCCAAGTTTCGCCTGCGTGAACCTTTCAAAAGGCCAATTTTAAGCGCTGAACCAGCAATTTCTGTGCATGAACTTCAACCGCATGATCAATTTCTCATATTTGCTTCGGATGGGCTCTGGGAGCACCTTACCAATCAGGAAGCCGTTGATATAGTTCAAAGTCACCCACACAGCGGAAGTGCTCGGAGGCTTGTCAAAGCTGCATTGCTGGAAGCGGCTAAGAAGAGAGAAATGAGATACTCCGATTTGAAGAAAATAGACCGTGGTGTCCGCAGGCATTTCCACGATGACATAACAGTCATAGTCGTATTTCTTGACTCAAACCTTGTGAGCAGAGCTAGCTCAACCAGGGGCCCTACTTTGTCTGTGAGAGGAGGTGGTATAAATCTACCCGCAAAAACTCTTGCCCCCATGGAACTGAATGCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

423

Amino Acids

46.11

Weight (kDa)

6.96

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 100 - 354 6e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1103, 1133
AccB7I CCANNNNNTGG 1 cut(s) 501
AccII CGCG 1 cut(s) 600
AciI CCGC 8 cut(s) 76, 354, 598, 917, 1011, 1052, 1108, 1230
AclI AACGTT 1 cut(s) 585
AclWI GGATC 3 cut(s) 276, 541, 777
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 670
AfaI GTAC 2 cut(s) 246, 639
AfeI AGCGCT 1 cut(s) 883
AfiI CCNNNNNNNGG 2 cut(s) 501, 1010
AgsI TTSAA 9 cut(s) 18, 220, 234, 403, 739, 865, 914, 995, 1087
AjiI CACGTC 1 cut(s) 414
AjnI CCWGG 2 cut(s) 154, 1184
AluBI AGCT 8 cut(s) 170, 280, 300, 527, 652, 1037, 1175, 1179
AluI AGCT 8 cut(s) 170, 280, 300, 527, 652, 1037, 1175, 1179
Alw21I GWGCWC 4 cut(s) 302, 666, 963, 1021
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 3 cut(s) 276, 541, 777
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 883
AoxI GGCC 7 cut(s) 151, 249, 313, 355, 503, 869, 1189
ApaI GGGCCC 1 cut(s) 1193
ApeKI GCWGC 3 cut(s) 167, 524, 1037
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 2 cut(s) 602, 884
AspS9I GGNCC 7 cut(s) 152, 290, 314, 356, 504, 1189, 1190
AsuHPI GGTGA 4 cut(s) 236, 377, 602, 992
AsuNHI GCTAGC 1 cut(s) 1175
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 1 cut(s) 290
BaeGI GKGCMC 2 cut(s) 351, 1193
BanII GRGCYC 3 cut(s) 302, 954, 1193
Bbv12I GWGCWC 4 cut(s) 302, 666, 963, 1021
BbvI GCAGC 3 cut(s) 179, 536, 1024
BccI CCATC 4 cut(s) 338, 467, 653, 941
BceAI ACGGC 2 cut(s) 367, 965
BciT130I CCWGG 2 cut(s) 156, 1186
BclI TGATCA 2 cut(s) 382, 922
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 2 cut(s) 780, 1176
BfoI RGCGCY 1 cut(s) 885
BglI GCCNNNNNGGC 1 cut(s) 321
BisI GCNGC 5 cut(s) 168, 355, 525, 1038, 1053
BlsI GCNGC 5 cut(s) 169, 356, 526, 1039, 1054
Bme1390I CCNGG 2 cut(s) 156, 1186
Bme18I GGWCC 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 812
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 7 cut(s) 152, 290, 314, 356, 504, 1189, 1190
BmiI GGNNCC 5 cut(s) 292, 316, 828, 1190, 1191
BmrFI CCNGG 2 cut(s) 156, 1186
BmsI GCATC 5 cut(s) 78, 128, 154, 687, 718
BmtI GCTAGC 1 cut(s) 1179
BpmI CTGGAG 1 cut(s) 651
BpuEI CTTGAG 4 cut(s) 284, 302, 316, 326
BsaBI GATNNNNATC 1 cut(s) 798
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 154, 1099, 1185, 1248
Bsc4I CCNNNNNNNGG 2 cut(s) 501, 1010
Bse1I ACTGG 1 cut(s) 634
Bse3DI GCAATG 2 cut(s) 576, 1040
Bse8I GATNNNNATC 1 cut(s) 798
BseBI CCWGG 2 cut(s) 156, 1186
BseDI CCNNGG 4 cut(s) 154, 1099, 1185, 1248
BseGI GGATG 6 cut(s) 119, 136, 445, 645, 709, 952
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 2 cut(s) 501, 1010
BseMI GCAATG 2 cut(s) 576, 1040
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 634
BseRI GAGGAG 1 cut(s) 1224
BseSI GKGCMC 2 cut(s) 351, 1193
BseXI GCAGC 3 cut(s) 179, 536, 1024
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 7 cut(s) 153, 251, 315, 357, 505, 871, 1191
BsiHKAI GWGCWC 4 cut(s) 302, 666, 963, 1021
BsiHKCI CYCGRG 1 cut(s) 812
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 2 cut(s) 501, 1010
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 276
BsmI GAATGC 2 cut(s) 700, 1264
BsnI GGCC 7 cut(s) 153, 251, 315, 357, 505, 871, 1191
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 812
Bsp120I GGGCCC 1 cut(s) 1189
Bsp1286I GDGCHC 7 cut(s) 302, 351, 666, 954, 963, 1021, 1193
Bsp1407I TGTACA 1 cut(s) 244
Bsp143I GATC 6 cut(s) 268, 382, 533, 555, 782, 922
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 8 cut(s) 76, 354, 598, 917, 1011, 1052, 1108, 1230
BspANI GGCC 7 cut(s) 153, 251, 315, 357, 505, 871, 1191
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 5 cut(s) 292, 316, 828, 1190, 1191
BspOI GCTAGC 1 cut(s) 1179
BspPI GGATC 3 cut(s) 276, 541, 777
BspTNI GGTCTC 1 cut(s) 356
BsrDI GCAATG 2 cut(s) 576, 1040
BsrGI TGTACA 1 cut(s) 244
BsrI ACTGG 1 cut(s) 634
BssECI CCNNGG 4 cut(s) 154, 1099, 1185, 1248
BssMI GATC 6 cut(s) 268, 382, 533, 555, 782, 922
BssT1I CCWWGG 1 cut(s) 1248
Bst2UI CCWGG 2 cut(s) 156, 1186
Bst4CI ACNGT 3 cut(s) 426, 1100, 1135
Bst6I CTCTTC 1 cut(s) 1055
BstAPI GCANNNNNTGC 1 cut(s) 706
BstAUI TGTACA 1 cut(s) 244
BstC8I GCNNGC 7 cut(s) 151, 172, 278, 302, 851, 1112, 1177
BstDEI CTNAG 2 cut(s) 360, 1056
BstDSI CCRYGG 2 cut(s) 1099, 1248
BstF5I GGATG 6 cut(s) 119, 136, 445, 645, 709, 952
BstFNI CGCG 1 cut(s) 600
BstH2I RGCGCY 1 cut(s) 885
BstHHI GCGC 2 cut(s) 602, 884
BstKTI GATC 6 cut(s) 271, 385, 536, 558, 785, 925
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 6 cut(s) 268, 382, 533, 555, 782, 922
BstMWI GCNNNNNNNGC 9 cut(s) 40, 146, 321, 461, 511, 706, 890, 958, 1176
BstNI CCWGG 2 cut(s) 156, 1186
BstSCI CCNGG 2 cut(s) 154, 1184
BstSLI GKGCMC 2 cut(s) 351, 1193
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 3 cut(s) 179, 536, 1024
BstX2I RGATCY 2 cut(s) 533, 782
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 2 cut(s) 533, 782
BsuRI GGCC 7 cut(s) 153, 251, 315, 357, 505, 871, 1191
BtgI CCRYGG 2 cut(s) 1099, 1248
BtrI CACGTC 1 cut(s) 414
BtsCI GGATG 6 cut(s) 119, 136, 445, 645, 709, 952
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 7 cut(s) 151, 172, 278, 302, 851, 1112, 1177
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 2 cut(s) 602, 884
Cfr13I GGNCC 7 cut(s) 152, 290, 314, 356, 504, 1189, 1190
Csp6I GTAC 2 cut(s) 245, 638
CviAII CATG 5 cut(s) 73, 430, 905, 920, 1249
CviQI GTAC 2 cut(s) 245, 638
DdeI CTNAG 2 cut(s) 360, 1056
DpnI GATC 6 cut(s) 270, 384, 535, 557, 784, 924
DpnII GATC 6 cut(s) 268, 382, 533, 555, 782, 922
DraIII CACNNNGTG 1 cut(s) 670
DrdI GACNNNNNNGTC 2 cut(s) 1103, 1133
DseDI GACNNNNNNGTC 2 cut(s) 1103, 1133
Eam1104I CTCTTC 1 cut(s) 1055
EarI CTCTTC 1 cut(s) 1055
Ecl136II GAGCTC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 1248
Eco24I GRGCYC 3 cut(s) 302, 954, 1193
Eco31I GGTCTC 1 cut(s) 356
Eco47I GGWCC 1 cut(s) 290
Eco47III AGCGCT 1 cut(s) 883
Eco53kI GAGCTC 1 cut(s) 300
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 812
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 2 cut(s) 1189, 1190
EcoRII CCWGG 2 cut(s) 154, 1184
EcoT14I CCWWGG 1 cut(s) 1248
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 3 cut(s) 302, 954, 1193
ErhI CCWWGG 1 cut(s) 1248
FaeI CATG 5 cut(s) 76, 433, 908, 923, 1252
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 1 cut(s) 276
FatI CATG 5 cut(s) 72, 429, 904, 919, 1248
FauI CCCGC 3 cut(s) 69, 605, 1237
FbaI TGATCA 2 cut(s) 382, 922
Fnu4HI GCNGC 5 cut(s) 168, 355, 525, 1038, 1053
FokI GGATG 6 cut(s) 106, 143, 452, 632, 696, 959
FriOI GRGCYC 3 cut(s) 302, 954, 1193
Fsp4HI GCNGC 5 cut(s) 168, 355, 525, 1038, 1053
FspBI CTAG 2 cut(s) 780, 1176
GlaI GCGC 2 cut(s) 601, 883
GluI GCNGC 5 cut(s) 168, 355, 525, 1038, 1053
GsuI CTGGAG 1 cut(s) 651
HaeII RGCGCY 1 cut(s) 885
HaeIII GGCC 7 cut(s) 153, 251, 315, 357, 505, 871, 1191
HhaI GCGC 2 cut(s) 602, 884
Hin1II CATG 5 cut(s) 76, 433, 908, 923, 1252
Hin6I GCGC 2 cut(s) 600, 882
HinP1I GCGC 2 cut(s) 600, 882
HinfI GANTC 7 cut(s) 284, 308, 477, 593, 680, 719, 1154
HphI GGTGA 4 cut(s) 236, 377, 602, 992
Hpy166II GTNNAC 2 cut(s) 411, 857
Hpy188I TCNGA 7 cut(s) 163, 273, 418, 689, 946, 1023, 1081
Hpy188III TCNNGA 6 cut(s) 56, 394, 713, 780, 974, 1151
Hpy8I GTNNAC 2 cut(s) 411, 857
HpyAV CCTTC 2 cut(s) 176, 616
HpyCH4III ACNGT 3 cut(s) 426, 1100, 1135
HpyCH4IV ACGT 2 cut(s) 413, 585
HpyCH4V TGCA 7 cut(s) 119, 167, 524, 700, 709, 904, 1040
HpyF10VI GCNNNNNNNGC 9 cut(s) 40, 146, 321, 461, 511, 706, 890, 958, 1176
HpyF3I CTNAG 2 cut(s) 360, 1056
HpySE526I ACGT 2 cut(s) 413, 585
Hsp92II CATG 5 cut(s) 76, 433, 908, 923, 1252
HspAI GCGC 2 cut(s) 600, 882
Ksp22I TGATCA 2 cut(s) 382, 922
Kzo9I GATC 6 cut(s) 268, 382, 533, 555, 782, 922
LmnI GCTCC 1 cut(s) 958
Lsp1109I GCAGC 3 cut(s) 179, 536, 1024
LweI GCATC 5 cut(s) 78, 128, 154, 687, 718
MaeI CTAG 2 cut(s) 780, 1176
MaeII ACGT 2 cut(s) 413, 585
MaeIII GTNAC 5 cut(s) 7, 224, 490, 590, 998
MalI GATC 6 cut(s) 270, 384, 535, 557, 784, 924
MboI GATC 6 cut(s) 268, 382, 533, 555, 782, 922
MboII GAAGA 4 cut(s) 39, 482, 1072, 1099
MflI RGATCY 2 cut(s) 533, 782
MhlI GDGCHC 7 cut(s) 302, 351, 666, 954, 963, 1021, 1193
MluCI AATT 6 cut(s) 256, 397, 519, 873, 894, 926
MlyI GAGTC 6 cut(s) 293, 317, 587, 689, 713, 1148
MmeI TCCRAC 2 cut(s) 85, 511
Mph1103I ATGCAT 1 cut(s) 702
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 2 cut(s) 819, 878
MslI CAYNNNNRTG 2 cut(s) 219, 434
MspA1I CMGCKG 4 cut(s) 170, 527, 652, 1011
MspR9I CCNGG 2 cut(s) 156, 1186
Mva1269I GAATGC 2 cut(s) 700, 1264
MvaI CCWGG 2 cut(s) 156, 1186
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 9 cut(s) 40, 146, 321, 461, 511, 706, 890, 958, 1176
NcoI CCATGG 1 cut(s) 1248
NdeII GATC 6 cut(s) 268, 382, 533, 555, 782, 922
NheI GCTAGC 1 cut(s) 1175
NlaIII CATG 5 cut(s) 76, 433, 908, 923, 1252
NlaIV GGNNCC 5 cut(s) 292, 316, 828, 1190, 1191
NmuCI GTSAC 4 cut(s) 7, 224, 590, 998
NsiI ATGCAT 1 cut(s) 702
PctI GAATGC 2 cut(s) 700, 1264
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 477
PflMI CCANNNNNTGG 1 cut(s) 501
PkrI GCNGC 5 cut(s) 169, 356, 526, 1039, 1054
PleI GAGTC 6 cut(s) 292, 316, 587, 688, 713, 1148
PpsI GAGTC 6 cut(s) 292, 316, 587, 688, 713, 1148
Psp124BI GAGCTC 1 cut(s) 302
Psp1406I AACGTT 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 154, 1184
PspGI CCWGG 2 cut(s) 154, 1184
PspN4I GGNNCC 5 cut(s) 292, 316, 828, 1190, 1191
PspOMI GGGCCC 1 cut(s) 1189
PspPI GGNCC 7 cut(s) 152, 290, 314, 356, 504, 1189, 1190
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 2 cut(s) 533, 782
PvuII CAGCTG 3 cut(s) 170, 527, 652
RsaI GTAC 2 cut(s) 246, 639
RsaNI GTAC 2 cut(s) 245, 638
RseI CAYNNNNRTG 2 cut(s) 219, 434
SacI GAGCTC 1 cut(s) 302
SaqAI TTAA 2 cut(s) 819, 878
SatI GCNGC 5 cut(s) 168, 355, 525, 1038, 1053
Sau3AI GATC 6 cut(s) 268, 382, 533, 555, 782, 922
Sau96I GGNCC 7 cut(s) 152, 290, 314, 356, 504, 1189, 1190
SchI GAGTC 6 cut(s) 293, 317, 587, 689, 713, 1148
ScrFI CCNGG 2 cut(s) 156, 1186
SduI GDGCHC 7 cut(s) 302, 351, 666, 954, 963, 1021, 1193
SfaNI GCATC 5 cut(s) 78, 128, 154, 687, 718
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 2 cut(s) 219, 434
SmlI CTYRAG 4 cut(s) 263, 281, 295, 305
SmoI CTYRAG 4 cut(s) 263, 281, 295, 305
Sse9I AATT 6 cut(s) 256, 397, 519, 873, 894, 926
SsiI CCGC 8 cut(s) 76, 354, 598, 917, 1011, 1052, 1108, 1230
SspMI CTAG 2 cut(s) 780, 1176
SstI GAGCTC 1 cut(s) 302
StyD4I CCNGG 2 cut(s) 154, 1184
StyI CCWWGG 1 cut(s) 1248
TaaI ACNGT 3 cut(s) 426, 1100, 1135
TaiI ACGT 2 cut(s) 416, 588
TasI AATT 6 cut(s) 256, 397, 519, 873, 894, 926
TatI WGTACW 1 cut(s) 244
TauI GCSGC 2 cut(s) 357, 1055
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 2 cut(s) 819, 878
Tru9I TTAA 2 cut(s) 819, 878
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 4 cut(s) 7, 224, 590, 998
TseI GCWGC 3 cut(s) 167, 524, 1037
Tsp45I GTSAC 4 cut(s) 7, 224, 590, 998
TspDTI ATGAA 1 cut(s) 921
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 1 cut(s) 441
Van91I CCANNNNNTGG 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 1 cut(s) 397
XbaI TCTAGA 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 284
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 2 cut(s) 780, 1176
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.