RchiOBHm_Chr6g0278751

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002066
Physical Location & Seq
Forward (+)
41865850 .. 41868895
3046 bp
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UTR
Exon/CDS
Intron
PRQ25004

Sequence Viewer

Length: 510 bp
ATGTTTGGCATTTCTAGATCTATTGGTGACTTCTATTTAAAGAAGGCTGAATTTAACAGGGAGCCTTTATATGCCAAGTTTCGTCTTCGGGACCCTATCAAAAGACCAATATTGAGCTCTGAGCCCGCAATTTCAGTGCTCCAATTGCAACCACATGATCAATTTATTATATTTGCTTCTGATGGGCTCTGGGAGCACCTCAGCAATCAGGAAGCAGTTGATATAGTTCAAAATCATCCCCGAAATGGAAGTGCAAAGCGGTTGGTGAAAACGGCACTGCAAGAAGCAGCTAAAAAGAGAGAAATGCGGTATTCAGACTTGAAGAAGATTGACCGTGGTGTTCGTCGCCATTTCCACGATGACATAACTGTAATTGTTGTGTTTCTCGACTCAAATCTTGTGAGCAAGGCTACTTCTGTCAAGTGCTCAGTGAGAGGGGGCGGTATCAGCCTGCCTCCTAATACTCTGGCTCCTTACACGACTACACCCACGGAAGCTGGCAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

169

Amino Acids

19.02

Weight (kDa)

9.67

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 3 - 98 1.1e-18 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 126, 259, 307, 441
AcsI RAATTY 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 2 cut(s) 230, 322
AluBI AGCT 3 cut(s) 117, 290, 497
AluI AGCT 3 cut(s) 117, 290, 497
Alw21I GWGCWC 4 cut(s) 119, 141, 198, 428
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 2 cut(s) 38, 277
AvaII GGWCC 1 cut(s) 91
BanII GRGCYC 3 cut(s) 119, 126, 189
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 4 cut(s) 119, 141, 198, 428
BbvCI CCTCAGC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 299
BccI CCATC 1 cut(s) 176
BceAI ACGGC 1 cut(s) 288
BclI TGATCA 1 cut(s) 157
BfaI CTAG 1 cut(s) 15
BglII AGATCT 1 cut(s) 17
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 4 cut(s) 63, 92, 93, 471
BpiI GAAGAC 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 334, 489
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 334, 489
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMII CTCAG 3 cut(s) 111, 214, 441
BseXI GCAGC 1 cut(s) 299
BsiHKAI GWGCWC 4 cut(s) 119, 141, 198, 428
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 104
Bsp1286I GDGCHC 6 cut(s) 119, 126, 141, 189, 198, 428
Bsp143I GATC 2 cut(s) 17, 157
BspACI CCGC 4 cut(s) 126, 259, 307, 441
BspCNI CTCAG 3 cut(s) 112, 213, 440
BspLI GGNNCC 4 cut(s) 63, 92, 93, 471
BssECI CCNNGG 2 cut(s) 334, 489
BssMI GATC 2 cut(s) 17, 157
Bst4CI ACNGT 2 cut(s) 335, 370
BstC8I GCNNGC 3 cut(s) 126, 452, 499
BstDEI CTNAG 3 cut(s) 120, 200, 427
BstDSI CCRYGG 2 cut(s) 334, 489
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 2 cut(s) 20, 160
BstMBI GATC 2 cut(s) 17, 157
BstMWI GCNNNNNNNGC 3 cut(s) 145, 193, 447
BstV1I GCAGC 1 cut(s) 299
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BtgI CCRYGG 2 cut(s) 334, 489
BtsCI GGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 3 cut(s) 141, 275, 435
Cac8I GCNNGC 3 cut(s) 126, 452, 499
Cfr13I GGNCC 1 cut(s) 91
CviAII CATG 1 cut(s) 155
DdeI CTNAG 3 cut(s) 120, 200, 427
DpnI GATC 2 cut(s) 19, 159
DpnII GATC 2 cut(s) 17, 157
DraI TTTAAA 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 117
Eco24I GRGCYC 3 cut(s) 119, 126, 189
Eco47I GGWCC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 91
EcoT38I GRGCYC 3 cut(s) 119, 126, 189
FaeI CATG 1 cut(s) 158
FaiI YATR 6 cut(s) 70, 72, 156, 170, 224, 365
FaqI GGGAC 1 cut(s) 104
FatI CATG 1 cut(s) 154
FauI CCCGC 1 cut(s) 133
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 222
FriOI GRGCYC 3 cut(s) 119, 126, 189
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 15
GluI GCNGC 1 cut(s) 288
Hin1II CATG 1 cut(s) 158
HinfI GANTC 1 cut(s) 389
HphI GGTGA 2 cut(s) 38, 277
Hpy188I TCNGA 3 cut(s) 121, 181, 316
Hpy188III TCNNGA 4 cut(s) 15, 89, 209, 386
Hpy99I CGWCG 1 cut(s) 348
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 2 cut(s) 335, 370
HpyCH4V TGCA 3 cut(s) 148, 254, 280
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 193, 447
HpyF3I CTNAG 3 cut(s) 120, 200, 427
Hsp92II CATG 1 cut(s) 158
KflI GGGWCCC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 2 cut(s) 17, 157
LmnI GCTCC 4 cut(s) 61, 144, 193, 475
LpnPI CCDG 6 cut(s) 43, 175, 194, 452, 464, 483
Lsp1109I GCAGC 1 cut(s) 299
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 26
MalI GATC 2 cut(s) 19, 159
MboI GATC 2 cut(s) 17, 157
MboII GAAGA 3 cut(s) 77, 334, 337
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 6 cut(s) 119, 126, 141, 189, 198, 428
MluCI AATT 5 cut(s) 50, 129, 143, 161, 372
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 3 cut(s) 209, 428, 465
MseI TTAA 2 cut(s) 38, 54
MunI CAATTG 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 145, 193, 447
NdeII GATC 2 cut(s) 17, 157
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 4 cut(s) 63, 92, 93, 471
NmuCI GTSAC 1 cut(s) 26
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PpuMI RGGWCCY 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 91
PspN4I GGNNCC 4 cut(s) 63, 92, 93, 471
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PsuI RGATCY 1 cut(s) 17
SacI GAGCTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 38, 54
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 2 cut(s) 17, 157
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 383
SduI GDGCHC 6 cut(s) 119, 126, 141, 189, 198, 428
SetI ASST 5 cut(s) 119, 201, 292, 499, 509
SinI GGWCC 1 cut(s) 91
Sse9I AATT 5 cut(s) 50, 129, 143, 161, 372
SsiI CCGC 4 cut(s) 126, 259, 307, 441
SspI AATATT 1 cut(s) 111
SspMI CTAG 1 cut(s) 15
SstI GAGCTC 1 cut(s) 119
TaaI ACNGT 2 cut(s) 335, 370
TaqI TCGA 1 cut(s) 387
TasI AATT 5 cut(s) 50, 129, 143, 161, 372
Tru1I TTAA 2 cut(s) 38, 54
Tru9I TTAA 2 cut(s) 38, 54
TscAI CASTG 3 cut(s) 141, 282, 435
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 26
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 3 cut(s) 141, 282, 435
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 1 cut(s) 50
XbaI TCTAGA 1 cut(s) 14
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.