Rh6DG226700

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002066
Physical Location & Seq
Forward (+)
40604112 .. 40609358
5247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG226700.1

Sequence Viewer

Length: 846 bp
ATGTCTGTGGATGTAATACGAAAAGCCTTTCAAGCAACCGAAGAGGGTTTTCTTTCTGTAGTTACCAGGCAGTGGCCTCTGAAACCACAAATTGCAGCAGTAGGATCCTGCTGCCTTGTTGGCGTTATTTGTAACGGAACTCTTTATATTGCCAACCTTGGTGATTCCCGTGCTGTTTTGGGGAGGATTGTGAAGGCAACAGGGGAGGTACTCTCAATTCAGTTGTCACAAGAGCACAATGCATGTATAGAATCTGTGAGACAGGAGCTGCATTCTTTGCACCCCGACGATCCACAGATTGTACTTTTGAAGCATAATGTTTGGCGTGTAAAGGGAGTAATACAGATTTCTAGATCTATTGGTGACTTCTATTTAAAGAAGGCTGAATTTAACAGGGAGCCTTTATATGCCAAGTTTCGTCTTCGGGACCCTATCAAAAGACCAATATTGAGCTCTGAGCCCGCAATTTCAGTGCTCCAATTGCAACCACATGATCAATTTATTATATTTGCTTCTGATGGGCTCTGGGAGCACCTCAGCAATCAGGAAGCAGTTGATATAGTTCAAAATCATCCCCGAAATGGAAGTGCAAAGCGGTTGGTGAAAACGGCACTGCAAGAAGCAGCTAAAAAGAGAGAAATGCGGTATTCAGACTTGAAGAAGATTGACCGTGGTGTTCGTCGCCATTTCCACGATGACATAACTGTAATTGTTGTGTTTCTCGACTCAAATCTTGTGAGCAAGGCTACTTCTGTCAAGTGCTCAGTGAGAGGGGGCGGTATCAGCCTGCCTCCTAATACTCTGGCTCCTTACACGACTACACCCACGGAAGCTGGCAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

281

Amino Acids

31.28

Weight (kDa)

9.3

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 4 - 209 3.3e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 72
AciI CCGC 4 cut(s) 462, 595, 643, 777
AclWI GGATC 3 cut(s) 99, 112, 284
AcsI RAATTY 1 cut(s) 386
AfaI GTAC 2 cut(s) 210, 303
AfiI CCNNNNNNNGG 2 cut(s) 72, 581
AgsI TTSAA 4 cut(s) 32, 310, 566, 658
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 4 cut(s) 268, 453, 626, 833
AluI AGCT 4 cut(s) 268, 453, 626, 833
Alw21I GWGCWC 5 cut(s) 237, 455, 477, 534, 764
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 3 cut(s) 99, 112, 284
AlwNI CAGNNNCTG 1 cut(s) 268
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 95, 111, 268, 623
ApoI RAATTY 1 cut(s) 386
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 3 cut(s) 173, 374, 613
AvaII GGWCC 1 cut(s) 427
BamHI GGATCC 1 cut(s) 104
BanII GRGCYC 3 cut(s) 455, 462, 525
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 5 cut(s) 237, 455, 477, 534, 764
BbvCI CCTCAGC 1 cut(s) 536
BbvI GCAGC 4 cut(s) 98, 107, 255, 635
BccI CCATC 1 cut(s) 512
BceAI ACGGC 1 cut(s) 624
BciT130I CCWGG 1 cut(s) 67
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 120
BglII AGATCT 1 cut(s) 353
BisI GCNGC 4 cut(s) 96, 112, 269, 624
BlsI GCNGC 4 cut(s) 97, 113, 270, 625
Bme1390I CCNGG 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 5 cut(s) 106, 399, 428, 429, 807
BmrFI CCNGG 1 cut(s) 67
BpiI GAAGAC 1 cut(s) 413
BplI GAGNNNNNCTC 2 cut(s) 197, 229
Bpu10I CCTNAGC 1 cut(s) 536
BsaJI CCNNGG 3 cut(s) 157, 670, 825
BsaXI ACNNNNNCTCC 2 cut(s) 790, 820
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 581
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 3 cut(s) 157, 670, 825
BseGI GGATG 2 cut(s) 16, 571
BseLI CCNNNNNNNGG 2 cut(s) 72, 581
BseMII CTCAG 3 cut(s) 447, 550, 777
BseXI GCAGC 4 cut(s) 98, 107, 255, 635
BshFI GGCC 1 cut(s) 76
BsiHKAI GWGCWC 5 cut(s) 237, 455, 477, 534, 764
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 72, 581
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 440
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 7 cut(s) 237, 455, 462, 477, 525, 534, 764
Bsp143I GATC 4 cut(s) 104, 289, 353, 493
BspACI CCGC 4 cut(s) 462, 595, 643, 777
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 3 cut(s) 448, 549, 776
BspLI GGNNCC 5 cut(s) 106, 399, 428, 429, 807
BspPI GGATC 3 cut(s) 99, 112, 284
BssECI CCNNGG 3 cut(s) 157, 670, 825
BssMI GATC 4 cut(s) 104, 289, 353, 493
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 2 cut(s) 671, 706
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 277
BstC8I GCNNGC 3 cut(s) 462, 788, 835
BstDEI CTNAG 3 cut(s) 456, 536, 763
BstDSI CCRYGG 2 cut(s) 670, 825
BstF5I GGATG 2 cut(s) 16, 571
BstKTI GATC 4 cut(s) 107, 292, 356, 496
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 4 cut(s) 104, 289, 353, 493
BstMWI GCNNNNNNNGC 6 cut(s) 32, 120, 277, 481, 529, 783
BstNI CCWGG 1 cut(s) 67
BstNSI RCATGY 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 65
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 4 cut(s) 98, 107, 255, 635
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 2 cut(s) 104, 353
BstYI RGATCY 2 cut(s) 104, 353
BsuRI GGCC 1 cut(s) 76
BtgI CCRYGG 2 cut(s) 670, 825
BtsCI GGATG 2 cut(s) 16, 571
BtsI GCAGTG 2 cut(s) 77, 611
BtsIMutI CAGTG 4 cut(s) 77, 477, 611, 771
Cac8I GCNNGC 3 cut(s) 462, 788, 835
CaiI CAGNNNCTG 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 209, 302
CviAII CATG 2 cut(s) 243, 491
CviQI GTAC 2 cut(s) 209, 302
DdeI CTNAG 3 cut(s) 456, 536, 763
DpnI GATC 4 cut(s) 106, 291, 355, 495
DpnII GATC 4 cut(s) 104, 289, 353, 493
DraI TTTAAA 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 157
Eco24I GRGCYC 3 cut(s) 455, 462, 525
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 453
EcoICRI GAGCTC 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 157
EcoT22I ATGCAT 1 cut(s) 244
EcoT38I GRGCYC 3 cut(s) 455, 462, 525
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 2 cut(s) 246, 494
FaqI GGGAC 1 cut(s) 440
FatI CATG 2 cut(s) 242, 490
FauI CCCGC 1 cut(s) 469
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 4 cut(s) 96, 112, 269, 624
FokI GGATG 2 cut(s) 23, 558
FriOI GRGCYC 3 cut(s) 455, 462, 525
Fsp4HI GCNGC 4 cut(s) 96, 112, 269, 624
FspBI CTAG 1 cut(s) 351
GluI GCNGC 4 cut(s) 96, 112, 269, 624
HaeIII GGCC 1 cut(s) 76
Hin1II CATG 2 cut(s) 246, 494
HinfI GANTC 3 cut(s) 164, 251, 725
HphI GGTGA 3 cut(s) 173, 374, 613
Hpy188I TCNGA 4 cut(s) 81, 457, 517, 652
Hpy188III TCNNGA 4 cut(s) 351, 425, 545, 722
Hpy99I CGWCG 2 cut(s) 290, 684
HpyAV CCTTC 2 cut(s) 187, 373
HpyCH4III ACNGT 2 cut(s) 671, 706
HpyCH4V TGCA 7 cut(s) 95, 242, 271, 280, 484, 590, 616
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 120, 277, 481, 529, 783
HpyF3I CTNAG 3 cut(s) 456, 536, 763
Hsp92II CATG 2 cut(s) 246, 494
KflI GGGWCCC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 4 cut(s) 104, 289, 353, 493
LmnI GCTCC 5 cut(s) 265, 397, 480, 529, 811
Lsp1109I GCAGC 4 cut(s) 98, 107, 255, 635
MaeI CTAG 1 cut(s) 351
MaeIII GTNAC 4 cut(s) 61, 131, 225, 362
MalI GATC 4 cut(s) 106, 291, 355, 495
MboI GATC 4 cut(s) 104, 289, 353, 493
MboII GAAGA 4 cut(s) 53, 413, 670, 673
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 2 cut(s) 104, 353
MhlI GDGCHC 7 cut(s) 237, 455, 462, 477, 525, 534, 764
MluCI AATT 7 cut(s) 90, 216, 386, 465, 479, 497, 708
MlyI GAGTC 1 cut(s) 719
MnlI CCTC 7 cut(s) 37, 87, 177, 199, 545, 764, 801
Mph1103I ATGCAT 1 cut(s) 244
MseI TTAA 2 cut(s) 374, 390
MspR9I CCNGG 1 cut(s) 67
MunI CAATTG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 1 cut(s) 67
MwoI GCNNNNNNNGC 6 cut(s) 32, 120, 277, 481, 529, 783
NdeII GATC 4 cut(s) 104, 289, 353, 493
NlaIII CATG 2 cut(s) 246, 494
NlaIV GGNNCC 5 cut(s) 106, 399, 428, 429, 807
NmuCI GTSAC 2 cut(s) 225, 362
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 1 cut(s) 246
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 164, 251
PflMI CCANNNNNTGG 1 cut(s) 72
PkrI GCNGC 4 cut(s) 97, 113, 270, 625
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 427
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 5 cut(s) 106, 399, 428, 429, 807
PspPI GGNCC 1 cut(s) 427
PspPPI RGGWCCY 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 2 cut(s) 104, 353
RsaI GTAC 2 cut(s) 210, 303
RsaNI GTAC 2 cut(s) 209, 302
SacI GAGCTC 1 cut(s) 455
SaqAI TTAA 2 cut(s) 374, 390
SatI GCNGC 4 cut(s) 96, 112, 269, 624
Sau3AI GATC 4 cut(s) 104, 289, 353, 493
Sau96I GGNCC 1 cut(s) 427
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 67
SduI GDGCHC 7 cut(s) 237, 455, 462, 477, 525, 534, 764
SetI ASST 8 cut(s) 159, 210, 270, 455, 537, 628, 835, 845
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 1 cut(s) 427
Sse9I AATT 7 cut(s) 90, 216, 386, 465, 479, 497, 708
SsiI CCGC 4 cut(s) 462, 595, 643, 777
SspI AATATT 1 cut(s) 447
SspMI CTAG 1 cut(s) 351
SstI GAGCTC 1 cut(s) 455
StyD4I CCNGG 1 cut(s) 65
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 2 cut(s) 671, 706
TaqI TCGA 1 cut(s) 723
TasI AATT 7 cut(s) 90, 216, 386, 465, 479, 497, 708
TatI WGTACW 1 cut(s) 301
TfiI GAWTC 2 cut(s) 164, 251
Tru1I TTAA 2 cut(s) 374, 390
Tru9I TTAA 2 cut(s) 374, 390
TscAI CASTG 4 cut(s) 77, 477, 618, 771
TseFI GTSAC 2 cut(s) 225, 362
TseI GCWGC 4 cut(s) 95, 111, 268, 623
Tsp45I GTSAC 2 cut(s) 225, 362
TspGWI ACGGA 2 cut(s) 150, 842
TspRI CASTG 4 cut(s) 77, 477, 618, 771
Van91I CCANNNNNTGG 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 427
XapI RAATTY 1 cut(s) 386
XbaI TCTAGA 1 cut(s) 350
XceI RCATGY 1 cut(s) 246
XspI CTAG 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.