MD10G1127000.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002066
Physical Location & Seq
Forward (+)
20708498 .. 20713186
4689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1127000.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGTTATCAGAGTTGATGAACTACTTGAAGTTCTGTTTTCGGCCGAGGTCGAATCGGTACGTTCATACCGGTGCCGATTCTGGGGGTCGCCAAGACGGGCTCCTGTGGTACAAAGACACGGGCCAGCACATAAATGGTGATTTCTCAATGGCTGTAGTTCAGGCTAACAATTTACTTGAGGATCAAAGTCAGCTTGAATCGGGTAGTTTGAGCACACATGAATCTGGCCCCTATGGTACTTTTGTTGGTGTTTATGACGGCCATGGGGGGCCGGAGACGTCACGCTATATCAATGATCACCTCTTTCAACATCTCAAGAGATTTAGTTCGGAGCAGCAGTCAATGTCTGTGGATGTAATACGAAAAGCATTTCAAGCAACAGAAGAGGGTTTTCTTAACGTAGTTACTAGGCAGTGGCCTATGAAACCACAGATTGCAGCAGTAGGATCGTGCTGCCTTGTCGGTGTTATTTGTAATGAAACTCTTTATATCGCCAACTGTGGTGATTCCCGTGCTGTTTTGGGGAGAATAGTGAAGGCAACAGGGGAGGTGCTCTCTATTCAGTTGTCACAAGAGCACAATGCCTGTATAGAATCTGTGAGACAGGAGCTGCAGTCTTTGCACCCTGATGATCCACATATCGTTGTTTTGAAGCATAATGTATGGCGTGTGAAAGGCCTAATACAGATATCTAGATCTATTGGTGATGTGTATTTGAAAAAGGCTGAATTTAACAGGGAGCCTTTGTATTCCAAGTTTCGTCTTCGGGACCCTATCAAAAGACCAATTTTGAGCGCTGATCCAGCAATTTCAGTGATCCAATTGCAGCCACACGATCAATTTATTATATTTGCATCTGATGGGCTATGGGAACACCTCACCAATCAGGAAGCAGTGGACATAGTTCAAAAACATCCACGAAGTGGAAGTGCAAAAAGGCTGGTGAAAACCGCCCTGCAAGAAGCAGCAAAAAAGAGAGAAATGAGGTATTCGGACTTGAAGAAGATTGACCGCGGGGTTCGCCGCCATTTCCATGATGACATAACCGTGATTGTTGTGTTTCTTGACTCGAACCTTGTGAGCAAGGCTAGTTCTGTCAAGGGCCCAAATCTATCGGTGAGAGGAGGTGGAATCAACCTGCCTCCTAACACTCTGGCTCCATACGCCTCGGCAACGGAAGCTGGCAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

398

Amino Acids

44.27

Weight (kDa)

8.62

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 77 - 324 3.2e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 1146
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 923
AccII CGCG 1 cut(s) 1014
AciI CCGC 4 cut(s) 951, 1012, 1014, 1024
AclWI GGATC 5 cut(s) 189, 454, 626, 794, 811
AcoI YGGCCR 2 cut(s) 41, 259
AcsI RAATTY 1 cut(s) 728
AcyI GRCGYC 1 cut(s) 278
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 3 cut(s) 59, 110, 238
AfeI AGCGCT 1 cut(s) 796
AfiI CCNNNNNNNGG 2 cut(s) 81, 923
AgeI ACCGGT 1 cut(s) 68
AgsI TTSAA 8 cut(s) 28, 197, 308, 374, 652, 718, 908, 1000
AluBI AGCT 3 cut(s) 193, 610, 1181
AluI AGCT 3 cut(s) 193, 610, 1181
Alw21I GWGCWC 3 cut(s) 215, 555, 579
Alw26I GTCTC 2 cut(s) 269, 595
AlwI GGATC 5 cut(s) 189, 454, 626, 794, 811
AlwNI CAGNNNCTG 1 cut(s) 610
Aor51HI AGCGCT 1 cut(s) 796
AoxI GGCC 8 cut(s) 41, 121, 226, 259, 269, 416, 676, 1102
ApaI GGGCCC 1 cut(s) 1106
ApeKI GCWGC 6 cut(s) 334, 437, 453, 610, 826, 965
ApoI RAATTY 1 cut(s) 728
AsiGI ACCGGT 1 cut(s) 68
AspLEI GCGC 1 cut(s) 797
AspS9I GGNCC 6 cut(s) 121, 227, 269, 769, 1102, 1103
AsuHPI GGTGA 7 cut(s) 149, 290, 515, 716, 871, 955, 1129
AvaII GGWCC 1 cut(s) 769
BaeGI GKGCMC 1 cut(s) 1106
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 102, 1106
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 3 cut(s) 215, 555, 579
BbvI GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
BccI CCATC 1 cut(s) 854
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 2 cut(s) 269, 595
BfaI CTAG 3 cut(s) 408, 693, 1089
BfmI CTRYAG 2 cut(s) 153, 611
BfoI RGCGCY 1 cut(s) 798
BfuAI ACCTGC 1 cut(s) 1146
BglII AGATCT 1 cut(s) 695
BisI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
BlsI GCNGC 7 cut(s) 336, 439, 455, 612, 828, 967, 1025
Bme18I GGWCC 1 cut(s) 769
BmgT120I GGNCC 6 cut(s) 121, 227, 269, 769, 1102, 1103
BmiI GGNNCC 9 cut(s) 73, 101, 229, 270, 741, 770, 771, 1104, 1158
BmsI GCATC 1 cut(s) 863
BpiI GAAGAC 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpuEI CTTGAG 2 cut(s) 197, 299
BsaHI GRCGYC 1 cut(s) 278
BsaJI CCNNGG 4 cut(s) 44, 262, 1012, 1167
BsaWI WCCGGW 1 cut(s) 68
BsaXI ACNNNNNCTCC 8 cut(s) 323, 353, 599, 629, 731, 761, 1141, 1171
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 923
Bse118I RCCGGY 1 cut(s) 68
BseDI CCNNGG 4 cut(s) 44, 262, 1012, 1167
BseGI GGATG 2 cut(s) 358, 913
BseLI CCNNNNNNNGG 2 cut(s) 81, 923
BseRI GAGGAG 1 cut(s) 1137
BseSI GKGCMC 1 cut(s) 1106
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
Bsh1236I CGCG 1 cut(s) 1014
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 8 cut(s) 43, 123, 228, 261, 271, 418, 678, 1104
BshNI GGYRCC 1 cut(s) 71
BshTI ACCGGT 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 3 cut(s) 215, 555, 579
BsiSI CCGG 2 cut(s) 69, 272
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 2 cut(s) 81, 923
BsmAI GTCTC 2 cut(s) 269, 595
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 782
BsnI GGCC 8 cut(s) 43, 123, 228, 261, 271, 418, 678, 1104
Bsp120I GGGCCC 1 cut(s) 1102
Bsp1286I GDGCHC 5 cut(s) 102, 215, 555, 579, 1106
Bsp143I GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 4 cut(s) 951, 1012, 1014, 1024
BspANI GGCC 8 cut(s) 43, 123, 228, 261, 271, 418, 678, 1104
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 9 cut(s) 73, 101, 229, 270, 741, 770, 771, 1104, 1158
BspMAI CTGCAG 1 cut(s) 615
BspMI ACCTGC 1 cut(s) 1146
BspPI GGATC 5 cut(s) 189, 454, 626, 794, 811
BspT107I GGYRCC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 4 cut(s) 44, 262, 1012, 1167
BssMI GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
BssNI GRCGYC 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 500, 1048
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 278
BstAPI GCANNNNNTGC 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 125, 1183
BstDSI CCRYGG 2 cut(s) 262, 1012
BstF5I GGATG 2 cut(s) 358, 913
BstFNI CGCG 1 cut(s) 1014
BstH2I RGCGCY 1 cut(s) 798
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 8 cut(s) 184, 298, 449, 634, 698, 802, 819, 838
BstMAI GTCTC 2 cut(s) 269, 595
BstMBI GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 6 cut(s) 374, 619, 803, 1020, 1163, 1178
BstSFI CTRYAG 2 cut(s) 153, 611
BstSLI GKGCMC 1 cut(s) 1106
BstUI CGCG 1 cut(s) 1014
BstV1I GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 8 cut(s) 43, 123, 228, 261, 271, 418, 678, 1104
BtgI CCRYGG 2 cut(s) 262, 1012
BtsCI GGATG 2 cut(s) 358, 913
BtsI GCAGTG 2 cut(s) 419, 900
BtsIMutI CAGTG 3 cut(s) 419, 819, 900
BveI ACCTGC 1 cut(s) 1146
Cac8I GCNNGC 2 cut(s) 125, 1183
CaiI CAGNNNCTG 1 cut(s) 610
CfoI GCGC 1 cut(s) 797
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 6 cut(s) 121, 227, 269, 769, 1102, 1103
Cfr42I CCGCGG 1 cut(s) 1015
Csp6I GTAC 3 cut(s) 58, 109, 237
CspAI ACCGGT 1 cut(s) 68
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CviAII CATG 3 cut(s) 218, 263, 1034
CviQI GTAC 3 cut(s) 58, 109, 237
DpnI GATC 8 cut(s) 183, 297, 448, 633, 697, 801, 818, 837
DpnII GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
DraIII CACNNNGTG 1 cut(s) 923
EaeI YGGCCR 2 cut(s) 41, 259
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 262
Eco147I AGGCCT 1 cut(s) 678
Eco24I GRGCYC 2 cut(s) 102, 1106
Eco32I GATATC 1 cut(s) 690
Eco47I GGWCC 1 cut(s) 769
Eco47III AGCGCT 1 cut(s) 796
Eco52I CGGCCG 1 cut(s) 41
EcoO109I RGGNCCY 2 cut(s) 769, 1102
EcoRV GATATC 1 cut(s) 690
EcoT14I CCWWGG 1 cut(s) 262
EcoT38I GRGCYC 2 cut(s) 102, 1106
ErhI CCWWGG 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 3 cut(s) 221, 266, 1037
FaqI GGGAC 1 cut(s) 782
FatI CATG 3 cut(s) 217, 262, 1033
FauI CCCGC 1 cut(s) 1007
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
FokI GGATG 2 cut(s) 365, 900
FriOI GRGCYC 2 cut(s) 102, 1106
Fsp4HI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
FspBI CTAG 3 cut(s) 408, 693, 1089
GlaI GCGC 1 cut(s) 796
GluI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
HaeII RGCGCY 1 cut(s) 798
HaeIII GGCC 8 cut(s) 43, 123, 228, 261, 271, 418, 678, 1104
HapII CCGG 2 cut(s) 69, 272
HhaI GCGC 1 cut(s) 797
Hin1I GRCGYC 1 cut(s) 278
Hin1II CATG 3 cut(s) 221, 266, 1037
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HinfI GANTC 8 cut(s) 52, 77, 197, 221, 506, 593, 1067, 1131
HpaII CCGG 2 cut(s) 69, 272
HphI GGTGA 7 cut(s) 149, 290, 515, 716, 871, 955, 1129
Hpy166II GTNNAC 1 cut(s) 898
Hpy188I TCNGA 4 cut(s) 10, 331, 859, 994
Hpy188III TCNNGA 5 cut(s) 316, 693, 767, 887, 1064
Hpy8I GTNNAC 1 cut(s) 898
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 2 cut(s) 500, 1048
HpyCH4IV ACGT 3 cut(s) 60, 278, 399
HpyCH4V TGCA 7 cut(s) 437, 613, 622, 826, 854, 932, 958
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 619, 803, 1020, 1163, 1178
HpySE526I ACGT 3 cut(s) 60, 278, 399
Hsp92I GRCGYC 1 cut(s) 278
Hsp92II CATG 3 cut(s) 221, 266, 1037
HspAI GCGC 1 cut(s) 795
KflI GGGWCCC 1 cut(s) 769
Ksp22I TGATCA 1 cut(s) 295
KspI CCGCGG 1 cut(s) 1015
Kzo9I GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
LmnI GCTCC 5 cut(s) 105, 331, 607, 739, 1162
Lsp1109I GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
LweI GCATC 1 cut(s) 863
MaeI CTAG 3 cut(s) 408, 693, 1089
MaeII ACGT 3 cut(s) 60, 278, 399
MaeIII GTNAC 3 cut(s) 279, 403, 567
MalI GATC 8 cut(s) 183, 297, 448, 633, 697, 801, 818, 837
MboI GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
MboII GAAGA 4 cut(s) 395, 755, 1012, 1015
MfeI CAATTG 1 cut(s) 821
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 5 cut(s) 102, 215, 555, 579, 1106
MluCI AATT 6 cut(s) 169, 728, 786, 807, 821, 839
MlyI GAGTC 1 cut(s) 1061
MseI TTAA 2 cut(s) 396, 732
MslI CAYNNNNRTG 5 cut(s) 69, 132, 627, 1032, 1046
MspA1I CMGCKG 1 cut(s) 1014
MspI CCGG 2 cut(s) 69, 272
MunI CAATTG 1 cut(s) 821
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 6 cut(s) 374, 619, 803, 1020, 1163, 1178
NcoI CCATGG 1 cut(s) 262
NdeII GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
NlaIII CATG 3 cut(s) 221, 266, 1037
NlaIV GGNNCC 9 cut(s) 73, 101, 229, 270, 741, 770, 771, 1104, 1158
NmeAIII GCCGAG 2 cut(s) 69, 1148
NmuCI GTSAC 2 cut(s) 279, 567
PceI AGGCCT 1 cut(s) 678
PfeI GAWTC 7 cut(s) 52, 77, 197, 221, 506, 593, 1131
PflMI CCANNNNNTGG 1 cut(s) 923
PinAI ACCGGT 1 cut(s) 68
PkrI GCNGC 7 cut(s) 336, 439, 455, 612, 828, 967, 1025
PleI GAGTC 1 cut(s) 1061
PpsI GAGTC 1 cut(s) 1061
PpuMI RGGWCCY 1 cut(s) 769
Psp5II RGGWCCY 1 cut(s) 769
PspN4I GGNNCC 9 cut(s) 73, 101, 229, 270, 741, 770, 771, 1104, 1158
PspOMI GGGCCC 1 cut(s) 1102
PspPI GGNCC 6 cut(s) 121, 227, 269, 769, 1102, 1103
PspPPI RGGWCCY 1 cut(s) 769
PstI CTGCAG 1 cut(s) 615
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 3 cut(s) 59, 110, 238
RsaNI GTAC 3 cut(s) 58, 109, 237
RseI CAYNNNNRTG 5 cut(s) 69, 132, 627, 1032, 1046
SacII CCGCGG 1 cut(s) 1015
SaqAI TTAA 2 cut(s) 396, 732
SatI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
Sau3AI GATC 8 cut(s) 181, 295, 446, 631, 695, 799, 816, 835
Sau96I GGNCC 6 cut(s) 121, 227, 269, 769, 1102, 1103
SchI GAGTC 1 cut(s) 1061
SduI GDGCHC 5 cut(s) 102, 215, 555, 579, 1106
SfaNI GCATC 1 cut(s) 863
SfcI CTRYAG 2 cut(s) 153, 611
Sfr303I CCGCGG 1 cut(s) 1015
SgrBI CCGCGG 1 cut(s) 1015
SinI GGWCC 1 cut(s) 769
SmiMI CAYNNNNRTG 5 cut(s) 69, 132, 627, 1032, 1046
SmlI CTYRAG 2 cut(s) 176, 314
SmoI CTYRAG 2 cut(s) 176, 314
Sse9I AATT 6 cut(s) 169, 728, 786, 807, 821, 839
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 4 cut(s) 951, 1012, 1014, 1024
SspMI CTAG 3 cut(s) 408, 693, 1089
StuI AGGCCT 1 cut(s) 678
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 2 cut(s) 500, 1048
TaiI ACGT 3 cut(s) 63, 281, 402
TaqI TCGA 2 cut(s) 50, 1070
TasI AATT 6 cut(s) 169, 728, 786, 807, 821, 839
TauI GCSGC 1 cut(s) 1026
TfiI GAWTC 7 cut(s) 52, 77, 197, 221, 506, 593, 1131
Tru1I TTAA 2 cut(s) 396, 732
Tru9I TTAA 2 cut(s) 396, 732
TscAI CASTG 3 cut(s) 419, 819, 900
TseFI GTSAC 2 cut(s) 279, 567
TseI GCWGC 6 cut(s) 334, 437, 453, 610, 826, 965
Tsp45I GTSAC 2 cut(s) 279, 567
TspDTI ATGAA 5 cut(s) 32, 53, 234, 437, 492
TspGWI ACGGA 1 cut(s) 1190
TspRI CASTG 3 cut(s) 419, 819, 900
Van91I CCANNNNNTGG 1 cut(s) 923
VpaK11BI GGWCC 1 cut(s) 769
XapI RAATTY 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 3 cut(s) 408, 693, 1089
ZraI GACGTC 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.