Rorug02G0073200

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002066
Physical Location & Seq
Forward (+)
5854229 .. 5858962
4734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0073200.1

Sequence Viewer

Length: 921 bp
ATGTTGAAGGATCTAGTGAGAACCAAGACGTATCAAAATGTTATTTATCAGAATAAGTTTCTATTCAAGGACAAAGTTGTTCTTGATGTCGGAGCTGGGACTGGAATTTTGTCTCTATTCTGTGCAAAAGCTGGTGCCAAGCATGTTTATGCAGTTGAGTGCTCAGATATGGCTGAGAGGGCAAAAGAGATTGTTGAAACTAATGGGTTTTCTAATGTTGTAACCGTTCTGAAAGGAAAGATTGAAGAACTTGAGCTTCCAGTTGCTAAAGTGGATATAATTATTTCTGAGTGGATGGGATACTTTTTGTTGTTTGAGAACATGTTGAATACCGTCCTCTATGCTCGTGATAAATGGCTTGTAGATGATGGAATTTTGCTACCGGACAAATGTTCTCTCTTTCTGACAGCTATTGAGGATGCAGAGTACAAAGAAGATAAGATAGAATTTTGGAATAATGTATATGGCTTTGACATGAGCTGCATCAAGAAGCAAGCCATGATGGAGCCTCTTGTGGACACGGTTGACCAAAACCAAATTGTTACTAATAGCCAGCTACTCAAGACAATGGATATATCTAAGATGACTCCTGGAGATGCTTCCTTCACAGCTCCTTTTAAGCTTATTGCTGAACGTGATGATTTCATTCATGCTCTTGTAGCCTACTTTGACGTGTCATTCACAAAATGTCATAAATTGATGGGCTTCTCCACAGGGCCAAGATCGCGATCTACACATTGGAAGCAAACAGTCCTGTACTTGGAAGATGTGCTGACAGTATGTCAAGGAGAGTCGATTGTTGGAACCATGACTGTGGCGCAAAACAAAAAGAATCCCCGTGATATTGATATAATGCTCAAGTATTCATTGAATGGTCAGCGATGCAGTGTCTCAAGGGTTCAATATTACAAGATGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

306

Amino Acids

34.86

Weight (kDa)

6.15

Isoelectric Point (pI)

26.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 22 - 97 2.2e-09 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 23 - 99 1.4e-08 Methyltransferase domain
Methyltransf_25 PF13649 27 - 100 5.2e-10 Methyltransferase domain
Methyltransf_11 PF08241 28 - 113 2.6e-08 Methyltransferase domain
PRMT_C PF22528 129 - 293 2.4e-59 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 727
AclWI GGATC 1 cut(s) 18
AcsI RAATTY 3 cut(s) 105, 372, 446
AfaI GTAC 2 cut(s) 428, 758
AfiI CCNNNNNNNGG 1 cut(s) 760
AflIII ACRYGT 2 cut(s) 321, 672
AgsI TTSAA 7 cut(s) 7, 67, 197, 245, 328, 871, 902
AhdI GACNNNNNGTC 1 cut(s) 780
AjiI CACGTC 1 cut(s) 673
AjnI CCWGG 1 cut(s) 589
AluBI AGCT 8 cut(s) 95, 131, 256, 410, 480, 556, 611, 622
AluI AGCT 8 cut(s) 95, 131, 256, 410, 480, 556, 611, 622
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 117, 895
AlwI GGATC 1 cut(s) 18
AoxI GGCC 1 cut(s) 716
ApeKI GCWGC 1 cut(s) 480
ApoI RAATTY 3 cut(s) 105, 372, 446
ArsI GACNNNNNNTTYG 2 cut(s) 91, 123
AspLEI GCGC 2 cut(s) 820, 918
AspS9I GGNCC 1 cut(s) 716
BanI GGYRCC 1 cut(s) 134
BauI CACGAG 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 467
BccI CCATC 4 cut(s) 289, 362, 496, 694
BciT130I CCWGG 1 cut(s) 591
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 117, 895
BfaI CTAG 1 cut(s) 14
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 1 cut(s) 481
BlsI GCNGC 1 cut(s) 482
Bme1390I CCNGG 1 cut(s) 591
BmeRI GACNNNNNGTC 1 cut(s) 780
BmgBI CACGTC 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 716
BmiI GGNNCC 3 cut(s) 136, 507, 805
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 5 cut(s) 409, 492, 586, 872, 903
BpmI CTGGAG 1 cut(s) 612
BpuEI CTTGAG 4 cut(s) 272, 545, 842, 877
BsaBI GATNNNNATC 1 cut(s) 727
BsaWI WCCGGW 1 cut(s) 382
Bsc4I CCNNNNNNNGG 1 cut(s) 760
Bse1I ACTGG 2 cut(s) 106, 260
Bse8I GATNNNNATC 1 cut(s) 727
BseBI CCWGG 1 cut(s) 591
BseGI GGATG 2 cut(s) 300, 424
BseJI GATNNNNATC 1 cut(s) 727
BseLI CCNNNNNNNGG 1 cut(s) 760
BseMII CTCAG 3 cut(s) 165, 177, 279
BseNI ACTGG 2 cut(s) 106, 260
BseXI GCAGC 1 cut(s) 467
BseYI CCCAGC 1 cut(s) 95
Bsh1236I CGCG 1 cut(s) 727
BshFI GGCC 1 cut(s) 718
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 383
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 760
BsmAI GTCTC 2 cut(s) 117, 895
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 1 cut(s) 718
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 3 cut(s) 10, 722, 728
Bsp68I TCGCGA 1 cut(s) 727
BspANI GGCC 1 cut(s) 718
BspCNI CTCAG 3 cut(s) 166, 176, 280
BspFNI CGCG 1 cut(s) 727
BspLI GGNNCC 3 cut(s) 136, 507, 805
BspPI GGATC 1 cut(s) 18
BspT107I GGYRCC 1 cut(s) 134
BsrI ACTGG 2 cut(s) 106, 260
BssMI GATC 3 cut(s) 10, 722, 728
BssSI CACGAG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 6 cut(s) 226, 334, 523, 751, 778, 814
BstC8I GCNNGC 2 cut(s) 495, 554
BstDEI CTNAG 4 cut(s) 163, 174, 288, 579
BstF5I GGATG 2 cut(s) 300, 424
BstFNI CGCG 1 cut(s) 727
BstHHI GCGC 2 cut(s) 820, 918
BstKTI GATC 3 cut(s) 13, 725, 731
BstMAI GTCTC 2 cut(s) 117, 895
BstMBI GATC 3 cut(s) 10, 722, 728
BstMWI GCNNNNNNNGC 3 cut(s) 179, 659, 724
BstNI CCWGG 1 cut(s) 591
BstNSI RCATGY 2 cut(s) 146, 325
BstSCI CCNGG 1 cut(s) 589
BstUI CGCG 1 cut(s) 727
BstV1I GCAGC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 10
BstXI CCANNNNNNTGG 1 cut(s) 814
BstYI RGATCY 1 cut(s) 10
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 718
BtgZI GCGATG 1 cut(s) 895
BtrI CACGTC 1 cut(s) 673
BtsCI GGATG 2 cut(s) 300, 424
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 1 cut(s) 892
BtuMI TCGCGA 1 cut(s) 727
Cac8I GCNNGC 2 cut(s) 495, 554
CfoI GCGC 2 cut(s) 820, 918
Cfr13I GGNCC 1 cut(s) 716
Csp6I GTAC 2 cut(s) 427, 757
CviAII CATG 6 cut(s) 143, 322, 475, 499, 650, 808
CviQI GTAC 2 cut(s) 427, 757
DdeI CTNAG 4 cut(s) 163, 174, 288, 579
DpnI GATC 3 cut(s) 12, 724, 730
DpnII GATC 3 cut(s) 10, 722, 728
DriI GACNNNNNGTC 1 cut(s) 780
Eam1105I GACNNNNNGTC 1 cut(s) 780
EcoRII CCWGG 1 cut(s) 589
FaeI CATG 6 cut(s) 146, 325, 478, 502, 653, 811
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 1 cut(s) 112
FatI CATG 6 cut(s) 142, 321, 474, 498, 649, 807
Fnu4HI GCNGC 1 cut(s) 481
FokI GGATG 2 cut(s) 307, 431
Fsp4HI GCNGC 1 cut(s) 481
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 819, 917
GluI GCNGC 1 cut(s) 481
GsaI CCCAGC 1 cut(s) 99
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 1 cut(s) 718
HapII CCGG 1 cut(s) 383
HhaI GCGC 2 cut(s) 820, 918
Hin1II CATG 6 cut(s) 146, 325, 478, 502, 653, 811
Hin6I GCGC 2 cut(s) 818, 916
HinP1I GCGC 2 cut(s) 818, 916
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 3 cut(s) 586, 791, 832
HpaII CCGG 1 cut(s) 383
Hpy166II GTNNAC 2 cut(s) 517, 526
Hpy188I TCNGA 6 cut(s) 51, 92, 166, 231, 289, 405
Hpy188III TCNNGA 5 cut(s) 83, 347, 487, 562, 726
Hpy8I GTNNAC 2 cut(s) 517, 526
HpyAV CCTTC 1 cut(s) 613
HpyCH4III ACNGT 6 cut(s) 226, 334, 523, 751, 778, 814
HpyCH4IV ACGT 3 cut(s) 29, 634, 672
HpyCH4V TGCA 5 cut(s) 125, 152, 422, 483, 885
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 659, 724
HpyF3I CTNAG 4 cut(s) 163, 174, 288, 579
HpySE526I ACGT 3 cut(s) 29, 634, 672
Hsp92II CATG 6 cut(s) 146, 325, 478, 502, 653, 811
HspAI GCGC 2 cut(s) 818, 916
Kzo9I GATC 3 cut(s) 10, 722, 728
LmnI GCTCC 3 cut(s) 92, 505, 616
Lsp1109I GCAGC 1 cut(s) 467
LweI GCATC 5 cut(s) 409, 492, 586, 872, 903
MaeI CTAG 1 cut(s) 14
MaeII ACGT 3 cut(s) 29, 634, 672
MaeIII GTNAC 2 cut(s) 220, 541
MalI GATC 3 cut(s) 12, 724, 730
MboI GATC 3 cut(s) 10, 722, 728
MboII GAAGA 3 cut(s) 257, 446, 776
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 6 cut(s) 105, 279, 372, 446, 537, 695
MlyI GAGTC 2 cut(s) 580, 800
MmeI TCCRAC 2 cut(s) 70, 781
MnlI CCTC 4 cut(s) 171, 347, 409, 519
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 2 cut(s) 147, 812
MspI CCGG 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 591
MvaI CCWGG 1 cut(s) 591
MvnI CGCG 1 cut(s) 727
MwoI GCNNNNNNNGC 3 cut(s) 179, 659, 724
NdeII GATC 3 cut(s) 10, 722, 728
NlaIII CATG 6 cut(s) 146, 325, 478, 502, 653, 811
NlaIV GGNNCC 3 cut(s) 136, 507, 805
NruI TCGCGA 1 cut(s) 727
NspI RCATGY 2 cut(s) 146, 325
PciI ACATGT 1 cut(s) 321
PfeI GAWTC 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 1 cut(s) 482
PleI GAGTC 2 cut(s) 580, 799
PpsI GAGTC 2 cut(s) 580, 799
PscI ACATGT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 589
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 3 cut(s) 136, 507, 805
PspPI GGNCC 1 cut(s) 716
PsuI RGATCY 1 cut(s) 10
RruI TCGCGA 1 cut(s) 727
RsaI GTAC 2 cut(s) 428, 758
RsaNI GTAC 2 cut(s) 427, 757
RseI CAYNNNNRTG 2 cut(s) 147, 812
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 1 cut(s) 481
Sau3AI GATC 3 cut(s) 10, 722, 728
Sau96I GGNCC 1 cut(s) 716
SchI GAGTC 2 cut(s) 580, 800
ScrFI CCNGG 1 cut(s) 591
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 5 cut(s) 409, 492, 586, 872, 903
SmiMI CAYNNNNRTG 2 cut(s) 147, 812
SmlI CTYRAG 4 cut(s) 251, 560, 857, 892
SmoI CTYRAG 4 cut(s) 251, 560, 857, 892
Sse9I AATT 6 cut(s) 105, 279, 372, 446, 537, 695
SspI AATATT 1 cut(s) 905
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 589
TaaI ACNGT 6 cut(s) 226, 334, 523, 751, 778, 814
TaiI ACGT 3 cut(s) 32, 637, 675
TaqI TCGA 1 cut(s) 794
TasI AATT 6 cut(s) 105, 279, 372, 446, 537, 695
TatI WGTACW 2 cut(s) 426, 756
TfiI GAWTC 1 cut(s) 832
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 1 cut(s) 892
TseI GCWGC 1 cut(s) 480
TspDTI ATGAA 3 cut(s) 634, 638, 855
TspRI CASTG 1 cut(s) 892
XapI RAATTY 3 cut(s) 105, 372, 446
XceI RCATGY 2 cut(s) 146, 325
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.