MD15G1238100.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002066
Physical Location & Seq
Reverse (-)
19598977 .. 19602480
3504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1238100.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGTTATCAAGGTTGATGAACATTCTGAGGGCCTGCTGGCTGCCTTCCTTGGACCGGTATGGACACGCAGGTTCGGATGCAGCTGGCAGACAAGAAGGGCTACTTTGGTACAAAGACACTGGGCAGCACATGAATGGTGAATTTTCAATGGCTGTTGTGCAGGCCAACAATTTGCTTGAGGATCAAAGCCAGGTTGAGTCTGGTCCCTTGAGCTCGCTCGAGTCTGGCCCGCATGGCACCTTTTTCGGAATATATGACGGTCATGGTGGTCCTGAGACCTCCCGTTACATCAATGATCACCTATTCCAGCATCTAAAACGGTTCACTTCAGAGCAACAGTCCATGTCAGTAGACGTGATGCGTAAAGCATATCAAGCAACAGAAGAGGGATTCCTCTCTGTTGTTACCAAACAATGGCCTATGAAACCCCAAATTGCAGCTGTTGGTTCTTGTTGCCTTGCTGGTGTTATTTGCAGTGGCACCCTTTACATTGCCAACGTTGGTGACTCCCGTGCTGTGCTGGGGAGAGTTATGAAGGCTACAGGGGAGGTTCGTGCCATCCAGTTGTCATCAGAGCACAATGTGGCCATAGAATCTGTCCGACAGGAAATGCATTCTTTGCACCCTGATGACTCACGTATTGTAGTTTTAAAGCATAACGTATGGCGTGTGAAGGGCTTGATACAGGTTTCTAGATCCATTGGTGATGTGTATCTTAAAAAGGCTGAATTTAACAGGGCGCCTTTGTATACCAAGTTTCGGTTGCGAGAACCTTTTAAAAGGCCAATTTTAAGCTCTGAACCATCGATCTCTGTGCATGAACTTCAACCACATGATCAGTTTCTCATACTTGCTTCCGATGGGCTGTGGGAGCACCTTACCAACCAGGAGGCGGTTGATATTGTTCAAAATCACCCACGCAGCGGAAGTGCTCGGAGGCTTGTGAAAACTGCCTTGCAGGAAGCAGCTAAGAAAAGAGAAATGAGATACTCAGACTTGAAGAAAATAGAGCGTGGTGTCCGCCGGCATTTCCACGACGACATAACGGTCATAGTCTTATTCCTTGACTCGAACCTTGTGAGGCGAGCCAGCTCAACGAGAGGCCCTACTTTGTCCGTGAGAGGAACCGGTGTTCATATACCATCAAAAACTCTTGCCCCATGTTCTACGCCCATGGATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

395

Amino Acids

44.05

Weight (kDa)

9.12

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 70 - 323 2.8e-41 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1046
Acc36I ACCTGC 1 cut(s) 59
AccB1I GGYRCC 3 cut(s) 236, 479, 739
AccB7I CCANNNNNTGG 1 cut(s) 414
AccI GTMKAC 2 cut(s) 351, 749
AciI CCGC 4 cut(s) 230, 893, 924, 1021
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 189, 690
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 2 cut(s) 140, 728
AcuI CTGAAG 1 cut(s) 312
AcyI GRCGYC 1 cut(s) 740
AdeI CACNNNGTG 1 cut(s) 583
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 5 cut(s) 54, 414, 759, 892, 923
AgeI ACCGGT 2 cut(s) 54, 1127
AgsI TTSAA 4 cut(s) 147, 827, 908, 1000
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 2 cut(s) 189, 885
AloI GAACNNNNNNTCC 2 cut(s) 1116, 1148
AluBI AGCT 6 cut(s) 83, 213, 440, 795, 968, 1092
AluI AGCT 6 cut(s) 83, 213, 440, 795, 968, 1092
Alw21I GWGCWC 4 cut(s) 215, 579, 876, 934
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 2 cut(s) 189, 690
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 7 cut(s) 30, 162, 226, 416, 585, 782, 1102
ApeKI GCWGC 6 cut(s) 40, 80, 124, 437, 921, 965
ApoI RAATTY 2 cut(s) 140, 728
AsiGI ACCGGT 2 cut(s) 54, 1127
AspLEI GCGC 1 cut(s) 742
AspS9I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
AsuHPI GGTGA 5 cut(s) 149, 290, 515, 716, 905
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 3 cut(s) 52, 203, 269
BalI TGGCCA 1 cut(s) 587
BanI GGYRCC 3 cut(s) 236, 479, 739
BanII GRGCYC 1 cut(s) 215
Bbv12I GWGCWC 4 cut(s) 215, 579, 876, 934
BbvI GCAGC 6 cut(s) 27, 92, 136, 449, 933, 977
BccI CCATC 4 cut(s) 566, 811, 854, 1150
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 796, 830
BciT130I CCWGG 2 cut(s) 191, 887
BciVI GTATCC 1 cut(s) 1171
BclI TGATCA 2 cut(s) 295, 835
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 743
BfuAI ACCTGC 1 cut(s) 59
BfuI GTATCC 1 cut(s) 1171
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 6 cut(s) 41, 81, 125, 438, 922, 966
BlsI GCNGC 6 cut(s) 42, 82, 126, 439, 923, 967
Bme1390I CCNGG 2 cut(s) 191, 887
Bme18I GGWCC 3 cut(s) 52, 203, 269
BmeT110I CYCGRG 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
BmiI GGNNCC 5 cut(s) 205, 238, 481, 741, 1126
BmrFI CCNGG 2 cut(s) 191, 887
BmrI ACTGGG 1 cut(s) 129
BmsI GCATC 3 cut(s) 67, 319, 348
BmuI ACTGGG 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 197, 229
Bsa29I ATCGAT 1 cut(s) 806
BsaAI YACGTR 1 cut(s) 638
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 48, 1173
BsaWI WCCGGW 2 cut(s) 54, 1127
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 414, 759, 892, 923
Bse118I RCCGGY 3 cut(s) 54, 1023, 1127
Bse1I ACTGG 2 cut(s) 124, 562
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 2 cut(s) 191, 887
BseCI ATCGAT 1 cut(s) 806
BseDI CCNNGG 2 cut(s) 48, 1173
BseGI GGATG 2 cut(s) 82, 558
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 5 cut(s) 54, 414, 759, 892, 923
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 3 cut(s) 17, 264, 1005
BseNI ACTGG 2 cut(s) 124, 562
BseXI GCAGC 6 cut(s) 27, 92, 136, 449, 933, 977
BseYI CCCAGC 1 cut(s) 520
BsgI GTGCAG 1 cut(s) 179
BshFI GGCC 7 cut(s) 32, 164, 228, 418, 587, 784, 1104
BshNI GGYRCC 3 cut(s) 236, 479, 739
BshTI ACCGGT 2 cut(s) 54, 1127
BshVI ATCGAT 1 cut(s) 806
BsiHKAI GWGCWC 4 cut(s) 215, 579, 876, 934
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 3 cut(s) 55, 1024, 1128
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 5 cut(s) 54, 414, 759, 892, 923
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 189
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 7 cut(s) 32, 164, 228, 418, 587, 784, 1104
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 4 cut(s) 215, 579, 876, 934
Bsp143I GATC 5 cut(s) 181, 295, 695, 807, 835
Bsp19I CCATGG 1 cut(s) 1173
BspACI CCGC 4 cut(s) 230, 893, 924, 1021
BspANI GGCC 7 cut(s) 32, 164, 228, 418, 587, 784, 1104
BspCNI CTCAG 3 cut(s) 18, 265, 1004
BspDI ATCGAT 1 cut(s) 806
BspLI GGNNCC 5 cut(s) 205, 238, 481, 741, 1126
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 189, 690
BspT107I GGYRCC 3 cut(s) 236, 479, 739
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 3 cut(s) 54, 1023, 1127
BsrI ACTGG 2 cut(s) 124, 562
BssAI RCCGGY 3 cut(s) 54, 1023, 1127
BssECI CCNNGG 2 cut(s) 48, 1173
BssMI GATC 5 cut(s) 181, 295, 695, 807, 835
BssNAI GTATAC 1 cut(s) 750
BssNI GRCGYC 1 cut(s) 740
BssT1I CCWWGG 2 cut(s) 48, 1173
Bst1107I GTATAC 1 cut(s) 750
Bst2UI CCWGG 2 cut(s) 191, 887
Bst4CI ACNGT 4 cut(s) 260, 321, 339, 1048
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 740
BstAPI GCANNNNNTGC 1 cut(s) 619
BstBAI YACGTR 1 cut(s) 638
BstC8I GCNNGC 9 cut(s) 34, 38, 85, 162, 215, 230, 1025, 1086, 1090
BstDEI CTNAG 4 cut(s) 26, 273, 969, 991
BstDSI CCRYGG 1 cut(s) 1173
BstF5I GGATG 2 cut(s) 82, 558
BstH2I RGCGCY 1 cut(s) 743
BstHHI GCGC 1 cut(s) 742
BstKTI GATC 5 cut(s) 184, 298, 698, 810, 838
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 5 cut(s) 181, 295, 695, 807, 835
BstMWI GCNNNNNNNGC 4 cut(s) 234, 374, 619, 871
BstNI CCWGG 2 cut(s) 191, 887
BstSCI CCNGG 2 cut(s) 189, 885
BstSFI CTRYAG 1 cut(s) 540
BstV1I GCAGC 6 cut(s) 27, 92, 136, 449, 933, 977
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BstZ17I GTATAC 1 cut(s) 750
Bsu15I ATCGAT 1 cut(s) 806
BsuI GTATCC 1 cut(s) 1171
BsuRI GGCC 7 cut(s) 32, 164, 228, 418, 587, 784, 1104
BsuTUI ATCGAT 1 cut(s) 806
BtgI CCRYGG 1 cut(s) 1173
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 2 cut(s) 82, 558
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 2 cut(s) 117, 481
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 9 cut(s) 34, 38, 85, 162, 215, 230, 1025, 1086, 1090
CfoI GCGC 1 cut(s) 742
Cfr10I RCCGGY 3 cut(s) 54, 1023, 1127
Cfr13I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
ClaI ATCGAT 1 cut(s) 806
Csp6I GTAC 1 cut(s) 109
CspAI ACCGGT 2 cut(s) 54, 1127
CviAII CATG 8 cut(s) 130, 233, 263, 343, 818, 833, 1161, 1174
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 4 cut(s) 26, 273, 969, 991
DinI GGCGCC 1 cut(s) 741
DpnI GATC 5 cut(s) 183, 297, 697, 809, 837
DpnII GATC 5 cut(s) 181, 295, 695, 807, 835
DraI TTTAAA 2 cut(s) 651, 778
DraIII CACNNNGTG 1 cut(s) 583
DrdI GACNNNNNNGTC 1 cut(s) 1046
DseDI GACNNNNNNGTC 1 cut(s) 1046
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 48, 1173
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 3 cut(s) 52, 203, 269
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 30, 1103
EcoRII CCWGG 2 cut(s) 189, 885
EcoT14I CCWWGG 2 cut(s) 48, 1173
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 215
EgeI GGCGCC 1 cut(s) 741
EheI GGCGCC 1 cut(s) 741
ErhI CCWWGG 2 cut(s) 48, 1173
FaeI CATG 8 cut(s) 133, 236, 266, 346, 821, 836, 1164, 1177
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 1 cut(s) 189
FatI CATG 8 cut(s) 129, 232, 262, 342, 817, 832, 1160, 1173
FauI CCCGC 1 cut(s) 237
FbaI TGATCA 2 cut(s) 295, 835
FblI GTMKAC 2 cut(s) 351, 749
Fnu4HI GCNGC 6 cut(s) 41, 81, 125, 438, 922, 966
FokI GGATG 2 cut(s) 89, 545
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 6 cut(s) 41, 81, 125, 438, 922, 966
FspBI CTAG 1 cut(s) 693
GlaI GCGC 1 cut(s) 741
GluI GCNGC 6 cut(s) 41, 81, 125, 438, 922, 966
GsaI CCCAGC 1 cut(s) 524
HaeII RGCGCY 1 cut(s) 743
HaeIII GGCC 7 cut(s) 32, 164, 228, 418, 587, 784, 1104
HapII CCGG 3 cut(s) 55, 1024, 1128
HhaI GCGC 1 cut(s) 742
Hin1I GRCGYC 1 cut(s) 740
Hin1II CATG 8 cut(s) 133, 236, 266, 346, 821, 836, 1164, 1177
Hin6I GCGC 1 cut(s) 740
HinP1I GCGC 1 cut(s) 740
HinfI GANTC 7 cut(s) 197, 221, 390, 506, 593, 632, 1067
HpaII CCGG 3 cut(s) 55, 1024, 1128
HphI GGTGA 5 cut(s) 149, 290, 515, 716, 905
Hpy166II GTNNAC 3 cut(s) 324, 352, 750
Hpy188III TCNNGA 2 cut(s) 272, 693
Hpy8I GTNNAC 3 cut(s) 324, 352, 750
Hpy99I CGWCG 1 cut(s) 1040
HpyAV CCTTC 4 cut(s) 54, 89, 529, 667
HpyCH4III ACNGT 4 cut(s) 260, 321, 339, 1048
HpyCH4IV ACGT 4 cut(s) 354, 498, 637, 660
HpyCH4V TGCA 8 cut(s) 80, 160, 437, 474, 613, 622, 817, 958
HpyF10VI GCNNNNNNNGC 4 cut(s) 234, 374, 619, 871
HpyF3I CTNAG 4 cut(s) 26, 273, 969, 991
HpySE526I ACGT 4 cut(s) 354, 498, 637, 660
Hsp92I GRCGYC 1 cut(s) 740
Hsp92II CATG 8 cut(s) 133, 236, 266, 346, 821, 836, 1164, 1177
HspAI GCGC 1 cut(s) 740
KasI GGCGCC 1 cut(s) 739
KroI GCCGGC 1 cut(s) 1023
KroNI GCCGGC 1 cut(s) 1025
Ksp22I TGATCA 2 cut(s) 295, 835
Kzo9I GATC 5 cut(s) 181, 295, 695, 807, 835
LmnI GCTCC 1 cut(s) 871
Lsp1109I GCAGC 6 cut(s) 27, 92, 136, 449, 933, 977
LweI GCATC 3 cut(s) 67, 319, 348
MaeI CTAG 1 cut(s) 693
MaeII ACGT 4 cut(s) 354, 498, 637, 660
MaeIII GTNAC 3 cut(s) 284, 403, 503
MalI GATC 5 cut(s) 183, 297, 697, 809, 837
MboI GATC 5 cut(s) 181, 295, 695, 807, 835
MboII GAAGA 2 cut(s) 395, 1012
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 4 cut(s) 215, 579, 876, 934
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 5 cut(s) 140, 169, 432, 728, 786
MluNI TGGCCA 1 cut(s) 587
Mly113I GGCGCC 1 cut(s) 740
MlyI GAGTC 5 cut(s) 206, 230, 500, 626, 1061
MmeI TCCRAC 1 cut(s) 625
Mox20I TGGCCA 1 cut(s) 587
Mph1103I ATGCAT 1 cut(s) 615
MroNI GCCGGC 1 cut(s) 1023
MscI TGGCCA 1 cut(s) 587
MseI TTAA 5 cut(s) 650, 717, 732, 777, 791
MslI CAYNNNNRTG 2 cut(s) 132, 627
Msp20I TGGCCA 1 cut(s) 587
MspA1I CMGCKG 3 cut(s) 83, 440, 924
MspI CCGG 3 cut(s) 55, 1024, 1128
MspR9I CCNGG 2 cut(s) 191, 887
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 191, 887
MwoI GCNNNNNNNGC 4 cut(s) 234, 374, 619, 871
NaeI GCCGGC 1 cut(s) 1025
NarI GGCGCC 1 cut(s) 740
NcoI CCATGG 1 cut(s) 1173
NdeII GATC 5 cut(s) 181, 295, 695, 807, 835
NgoMIV GCCGGC 1 cut(s) 1023
NlaIII CATG 8 cut(s) 133, 236, 266, 346, 821, 836, 1164, 1177
NlaIV GGNNCC 5 cut(s) 205, 238, 481, 741, 1126
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 615
PaeR7I CTCGAG 1 cut(s) 218
PctI GAATGC 1 cut(s) 613
PdiI GCCGGC 1 cut(s) 1025
PfeI GAWTC 2 cut(s) 390, 593
PflMI CCANNNNNTGG 1 cut(s) 414
PinAI ACCGGT 2 cut(s) 54, 1127
PkrI GCNGC 6 cut(s) 42, 82, 126, 439, 923, 967
PleI GAGTC 5 cut(s) 205, 229, 500, 626, 1061
PluTI GGCGCC 1 cut(s) 743
PpsI GAGTC 5 cut(s) 205, 229, 500, 626, 1061
Ppu21I YACGTR 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 215
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 189, 885
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 189, 885
PspN4I GGNNCC 5 cut(s) 205, 238, 481, 741, 1126
PspPI GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 1 cut(s) 695
PvuII CAGCTG 2 cut(s) 83, 440
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 2 cut(s) 132, 627
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 5 cut(s) 650, 717, 732, 777, 791
SatI GCNGC 6 cut(s) 41, 81, 125, 438, 922, 966
Sau3AI GATC 5 cut(s) 181, 295, 695, 807, 835
Sau96I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
SchI GAGTC 5 cut(s) 206, 230, 500, 626, 1061
ScrFI CCNGG 2 cut(s) 191, 887
SduI GDGCHC 4 cut(s) 215, 579, 876, 934
SfaNI GCATC 3 cut(s) 67, 319, 348
SfcI CTRYAG 1 cut(s) 540
SfoI GGCGCC 1 cut(s) 741
Sfr274I CTCGAG 1 cut(s) 218
SinI GGWCC 3 cut(s) 52, 203, 269
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 132, 627
SmlI CTYRAG 3 cut(s) 176, 208, 218
SmoI CTYRAG 3 cut(s) 176, 208, 218
Sse9I AATT 5 cut(s) 140, 169, 432, 728, 786
SsiI CCGC 4 cut(s) 230, 893, 924, 1021
SspDI GGCGCC 1 cut(s) 739
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 189, 885
StyI CCWWGG 2 cut(s) 48, 1173
TaaI ACNGT 4 cut(s) 260, 321, 339, 1048
TaiI ACGT 4 cut(s) 357, 501, 640, 663
TaqI TCGA 3 cut(s) 219, 806, 1070
TasI AATT 5 cut(s) 140, 169, 432, 728, 786
TfiI GAWTC 2 cut(s) 390, 593
Tru1I TTAA 5 cut(s) 650, 717, 732, 777, 791
Tru9I TTAA 5 cut(s) 650, 717, 732, 777, 791
TscAI CASTG 2 cut(s) 124, 481
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 6 cut(s) 40, 80, 124, 437, 921, 965
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 6 cut(s) 32, 146, 437, 548, 834, 1124
TspGWI ACGGA 1 cut(s) 1105
TspRI CASTG 2 cut(s) 124, 481
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 3 cut(s) 52, 203, 269
XapI RAATTY 2 cut(s) 140, 728
XbaI TCTAGA 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 2 cut(s) 351, 749
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.