MD02G1124100.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002066
Physical Location & Seq
Reverse (-)
10104504 .. 10108178
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1124100.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGTTATCAAGGTTGATGAACTTTCTGAGGGCCTGTTGGCTGCCTTCCTCGGACCAGTATGGACACGCAGGTTCGGATGCAGCTGGCAGACAAGACGGGCTACTTTGGTACAAAGACATTGGGCAGCACATGAATGGTGAATTTTCAATGGCTGTTGTGCAGGCCAACAATTTGCTTGAGGATCAAAGCCAGGTTGAGTCTGGTCCCCTGAGCTCGCTCGAGTCTGGCCCGTATGGCACCTTTTTCGGAATATATGACGGTCATGGCGGTCCTGAGACCTCACGTTACATCAATGATCACCTATTCCAGCATCTAAAAAGGTTCACTTCAGAGCAACAGTCCATGTCAGTAGACGTGATACGGAAAGCATATCAAGCAACAGAAGAGGGGTTCCTCTCTGTTGTTACCAAACAATGGCCTATGAAACCCCAAATTGCAGCTGTTGGATCTTGTTGCCTTGCTGGTGTGATTTGTGGTGGCACCCTTTACATTGCCAACGTTGGTGATTCCCGTGCTGTTCTGGGGAGAGTTATGAAGGCTACTGGGGAGGTTCGTGCCATCCAGTTGTCATCAGAGCACAATGTGGCCATAGAATCTGTCAGACGGGAGATGCATTCTTTGCACCCTGATGACTCACATATTGTAGTTTTAAAGCATAATGTATGGCGCGTGAAGGGCTTGATACAGGTTTCTAGATCTATTGGTGATGTATATCTTAAAAAGCCTGAATTTAACAGGGAGCCTTTATATGCCAAGTTTCGCTTGCGCGAACCTTTTAAAAGGCCAATTTTAAGCTCTGAACCATCGATCTCTGTACATGAACTTCAACCGCACGATCAATTTCTCATACTTGCATCTGATGGGCTGTGGGAGCACCTCACCAACCAGGAGGCAGTTGATATTGTTCAAAATCACCCACACAGCGGAAGTGCTCGGAGGCTTCTGAAAACTGCCTTGCAGGAAGCAGCTAAGAAAAGAGAAATGAGATATTCAGACTTGAAGAAAATAGAGCGTGGTGTCCGCCGGCATTTCCACGACGACATAACAGTGATAGTCCTATTCCTTGACTCGAACCTTGTGAGCAAAGCCAGCTCAACGAGAGGTCCTTCTTTGTCCGTGAGAGGAGCCGGTGTTCATATACCATCAAAAACTCTTGCCCCATGTTCCACACCCATGGATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

395

Amino Acids

43.9

Weight (kDa)

8.5

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 78 - 323 6e-41 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
AccB1I GGYRCC 2 cut(s) 236, 479
AccB7I CCANNNNNTGG 1 cut(s) 414
AccI GTMKAC 1 cut(s) 351
AccII CGCG 2 cut(s) 669, 768
AciI CCGC 4 cut(s) 267, 830, 924, 1021
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 189, 454
AcoI YGGCCR 1 cut(s) 585
AcsI RAATTY 2 cut(s) 140, 728
AcuI CTGAAG 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 583
AfaI GTAC 2 cut(s) 110, 816
AfiI CCNNNNNNNGG 2 cut(s) 414, 923
AgsI TTSAA 4 cut(s) 147, 827, 908, 1000
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 2 cut(s) 189, 885
AloI GAACNNNNNNTCC 2 cut(s) 1116, 1148
AluBI AGCT 7 cut(s) 83, 213, 440, 795, 968, 1092, 1182
AluI AGCT 7 cut(s) 83, 213, 440, 795, 968, 1092, 1182
Alw21I GWGCWC 4 cut(s) 215, 579, 876, 934
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 2 cut(s) 189, 454
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 6 cut(s) 30, 162, 226, 416, 585, 782
ApeKI GCWGC 5 cut(s) 40, 80, 124, 437, 965
ApoI RAATTY 2 cut(s) 140, 728
AspLEI GCGC 2 cut(s) 669, 768
AspS9I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
AsuHPI GGTGA 6 cut(s) 149, 290, 515, 716, 871, 905
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 4 cut(s) 52, 203, 269, 1103
BalI TGGCCA 1 cut(s) 587
BanI GGYRCC 2 cut(s) 236, 479
BanII GRGCYC 1 cut(s) 215
BarI GAAGNNNNNNTAC 2 cut(s) 807, 839
Bbv12I GWGCWC 4 cut(s) 215, 579, 876, 934
BbvI GCAGC 5 cut(s) 27, 92, 136, 449, 977
BccI CCATC 4 cut(s) 566, 811, 854, 1150
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 2 cut(s) 191, 887
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 1 cut(s) 693
BfuAI ACCTGC 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 234
BglII AGATCT 1 cut(s) 695
BisI GCNGC 5 cut(s) 41, 81, 125, 438, 966
BlsI GCNGC 5 cut(s) 42, 82, 126, 439, 967
Bme1390I CCNGG 2 cut(s) 191, 887
Bme18I GGWCC 4 cut(s) 52, 203, 269, 1103
BmeT110I CYCGRG 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 355
BmgT120I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
BmiI GGNNCC 6 cut(s) 205, 238, 392, 481, 741, 1126
BmrFI CCNGG 2 cut(s) 191, 887
BmrI ACTGGG 1 cut(s) 552
BmsI GCATC 4 cut(s) 67, 319, 600, 863
BmuI ACTGGG 1 cut(s) 552
Bpu10I CCTNAGC 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 197
Bsa29I ATCGAT 1 cut(s) 806
BsaBI GATNNNNATC 1 cut(s) 711
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 48, 1173
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 923
Bse118I RCCGGY 2 cut(s) 1023, 1127
Bse1I ACTGG 3 cut(s) 55, 547, 562
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 2 cut(s) 191, 887
BseCI ATCGAT 1 cut(s) 806
BseDI CCNNGG 2 cut(s) 48, 1173
BseGI GGATG 2 cut(s) 82, 558
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 2 cut(s) 414, 923
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 3 cut(s) 17, 200, 264
BseNI ACTGG 3 cut(s) 55, 547, 562
BseRI GAGGAG 1 cut(s) 1137
BseXI GCAGC 5 cut(s) 27, 92, 136, 449, 977
BsgI GTGCAG 1 cut(s) 179
Bsh1236I CGCG 2 cut(s) 669, 768
BshFI GGCC 6 cut(s) 32, 164, 228, 418, 587, 784
BshNI GGYRCC 2 cut(s) 236, 479
BshVI ATCGAT 1 cut(s) 806
BsiHKAI GWGCWC 4 cut(s) 215, 579, 876, 934
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 2 cut(s) 1024, 1128
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 2 cut(s) 414, 923
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 189
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 6 cut(s) 32, 164, 228, 418, 587, 784
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 4 cut(s) 215, 579, 876, 934
Bsp1407I TGTACA 1 cut(s) 814
Bsp143I GATC 6 cut(s) 181, 295, 446, 695, 807, 835
Bsp19I CCATGG 1 cut(s) 1173
BspACI CCGC 4 cut(s) 267, 830, 924, 1021
BspANI GGCC 6 cut(s) 32, 164, 228, 418, 587, 784
BspCNI CTCAG 3 cut(s) 18, 201, 265
BspDI ATCGAT 1 cut(s) 806
BspFNI CGCG 2 cut(s) 669, 768
BspLI GGNNCC 6 cut(s) 205, 238, 392, 481, 741, 1126
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 189, 454
BspT107I GGYRCC 2 cut(s) 236, 479
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 2 cut(s) 1023, 1127
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 3 cut(s) 55, 547, 562
BssAI RCCGGY 2 cut(s) 1023, 1127
BssECI CCNNGG 2 cut(s) 48, 1173
BssMI GATC 6 cut(s) 181, 295, 446, 695, 807, 835
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 2 cut(s) 191, 887
Bst4CI ACNGT 3 cut(s) 260, 339, 1048
Bst6I CTCTTC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 619
BstAUI TGTACA 1 cut(s) 814
BstC8I GCNNGC 6 cut(s) 85, 162, 215, 764, 1025, 1090
BstDEI CTNAG 4 cut(s) 26, 209, 273, 969
BstDSI CCRYGG 1 cut(s) 1173
BstF5I GGATG 2 cut(s) 82, 558
BstFNI CGCG 2 cut(s) 669, 768
BstHHI GCGC 2 cut(s) 669, 768
BstKTI GATC 6 cut(s) 184, 298, 449, 698, 810, 838
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 6 cut(s) 181, 295, 446, 695, 807, 835
BstMWI GCNNNNNNNGC 6 cut(s) 234, 374, 619, 675, 871, 1089
BstNI CCWGG 2 cut(s) 191, 887
BstSCI CCNGG 2 cut(s) 189, 885
BstUI CGCG 2 cut(s) 669, 768
BstV1I GCAGC 5 cut(s) 27, 92, 136, 449, 977
BstX2I RGATCY 2 cut(s) 446, 695
BstXI CCANNNNNNTGG 1 cut(s) 1174
BstYI RGATCY 2 cut(s) 446, 695
Bsu15I ATCGAT 1 cut(s) 806
BsuRI GGCC 6 cut(s) 32, 164, 228, 418, 587, 784
BsuTUI ATCGAT 1 cut(s) 806
BtgI CCRYGG 1 cut(s) 1173
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 2 cut(s) 82, 558
BtsIMutI CAGTG 1 cut(s) 1053
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 6 cut(s) 85, 162, 215, 764, 1025, 1090
CfoI GCGC 2 cut(s) 669, 768
Cfr10I RCCGGY 2 cut(s) 1023, 1127
Cfr13I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
ClaI ATCGAT 1 cut(s) 806
Csp6I GTAC 2 cut(s) 109, 815
CviAII CATG 6 cut(s) 130, 263, 343, 818, 1161, 1174
CviQI GTAC 2 cut(s) 109, 815
DdeI CTNAG 4 cut(s) 26, 209, 273, 969
DpnI GATC 6 cut(s) 183, 297, 448, 697, 809, 837
DpnII GATC 6 cut(s) 181, 295, 446, 695, 807, 835
DraI TTTAAA 2 cut(s) 651, 778
DraIII CACNNNGTG 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 1173
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 4 cut(s) 52, 203, 269, 1103
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 30, 1103
EcoRII CCWGG 2 cut(s) 189, 885
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 1173
FaeI CATG 6 cut(s) 133, 266, 346, 821, 1164, 1177
FalI AAGNNNNNCTT 4 cut(s) 310, 342, 746, 778
FaqI GGGAC 1 cut(s) 189
FatI CATG 6 cut(s) 129, 262, 342, 817, 1160, 1173
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 5 cut(s) 41, 81, 125, 438, 966
FokI GGATG 2 cut(s) 89, 545
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 5 cut(s) 41, 81, 125, 438, 966
FspBI CTAG 1 cut(s) 693
GlaI GCGC 2 cut(s) 668, 767
GluI GCNGC 5 cut(s) 41, 81, 125, 438, 966
HaeIII GGCC 6 cut(s) 32, 164, 228, 418, 587, 784
HapII CCGG 2 cut(s) 1024, 1128
HhaI GCGC 2 cut(s) 669, 768
Hin1II CATG 6 cut(s) 133, 266, 346, 821, 1164, 1177
Hin6I GCGC 2 cut(s) 667, 766
HinP1I GCGC 2 cut(s) 667, 766
HinfI GANTC 6 cut(s) 197, 221, 506, 593, 632, 1067
HpaII CCGG 2 cut(s) 1024, 1128
HphI GGTGA 6 cut(s) 149, 290, 515, 716, 871, 905
Hpy166II GTNNAC 2 cut(s) 324, 352
Hpy188III TCNNGA 2 cut(s) 272, 693
Hpy8I GTNNAC 2 cut(s) 324, 352
Hpy99I CGWCG 1 cut(s) 1040
HpyAV CCTTC 4 cut(s) 54, 529, 667, 1116
HpyCH4III ACNGT 3 cut(s) 260, 339, 1048
HpyCH4IV ACGT 3 cut(s) 283, 354, 498
HpyCH4V TGCA 7 cut(s) 80, 160, 437, 613, 622, 854, 958
HpyF10VI GCNNNNNNNGC 6 cut(s) 234, 374, 619, 675, 871, 1089
HpyF3I CTNAG 4 cut(s) 26, 209, 273, 969
HpySE526I ACGT 3 cut(s) 283, 354, 498
Hsp92II CATG 6 cut(s) 133, 266, 346, 821, 1164, 1177
HspAI GCGC 2 cut(s) 667, 766
KroI GCCGGC 1 cut(s) 1023
KroNI GCCGGC 1 cut(s) 1025
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 6 cut(s) 181, 295, 446, 695, 807, 835
LmnI GCTCC 3 cut(s) 739, 871, 1124
Lsp1109I GCAGC 5 cut(s) 27, 92, 136, 449, 977
LweI GCATC 4 cut(s) 67, 319, 600, 863
MaeI CTAG 1 cut(s) 693
MaeII ACGT 3 cut(s) 283, 354, 498
MaeIII GTNAC 2 cut(s) 284, 403
MalI GATC 6 cut(s) 183, 297, 448, 697, 809, 837
MboI GATC 6 cut(s) 181, 295, 446, 695, 807, 835
MboII GAAGA 2 cut(s) 395, 1012
MflI RGATCY 2 cut(s) 446, 695
MhlI GDGCHC 4 cut(s) 215, 579, 876, 934
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 6 cut(s) 140, 169, 432, 728, 786, 839
MluNI TGGCCA 1 cut(s) 587
MlyI GAGTC 4 cut(s) 206, 230, 626, 1061
MmeI TCCRAC 1 cut(s) 424
Mox20I TGGCCA 1 cut(s) 587
Mph1103I ATGCAT 1 cut(s) 615
MroNI GCCGGC 1 cut(s) 1023
MscI TGGCCA 1 cut(s) 587
MseI TTAA 5 cut(s) 650, 717, 732, 777, 791
MslI CAYNNNNRTG 4 cut(s) 132, 627, 1046, 1172
Msp20I TGGCCA 1 cut(s) 587
MspA1I CMGCKG 3 cut(s) 83, 440, 924
MspI CCGG 2 cut(s) 1024, 1128
MspR9I CCNGG 2 cut(s) 191, 887
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 191, 887
MvnI CGCG 2 cut(s) 669, 768
MwoI GCNNNNNNNGC 6 cut(s) 234, 374, 619, 675, 871, 1089
NaeI GCCGGC 1 cut(s) 1025
NcoI CCATGG 1 cut(s) 1173
NdeII GATC 6 cut(s) 181, 295, 446, 695, 807, 835
NgoMIV GCCGGC 1 cut(s) 1023
NlaIII CATG 6 cut(s) 133, 266, 346, 821, 1164, 1177
NlaIV GGNNCC 6 cut(s) 205, 238, 392, 481, 741, 1126
NsiI ATGCAT 1 cut(s) 615
PaeR7I CTCGAG 1 cut(s) 218
PctI GAATGC 1 cut(s) 613
PdiI GCCGGC 1 cut(s) 1025
PfeI GAWTC 2 cut(s) 506, 593
PflMI CCANNNNNTGG 1 cut(s) 414
PkrI GCNGC 5 cut(s) 42, 82, 126, 439, 967
PleI GAGTC 4 cut(s) 205, 229, 626, 1061
PpsI GAGTC 4 cut(s) 205, 229, 626, 1061
PpuMI RGGWCCY 1 cut(s) 1103
Psp124BI GAGCTC 1 cut(s) 215
Psp1406I AACGTT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 1103
Psp6I CCWGG 2 cut(s) 189, 885
PspGI CCWGG 2 cut(s) 189, 885
PspN4I GGNNCC 6 cut(s) 205, 238, 392, 481, 741, 1126
PspPI GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
PspPPI RGGWCCY 1 cut(s) 1103
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 2 cut(s) 446, 695
PvuII CAGCTG 2 cut(s) 83, 440
RsaI GTAC 2 cut(s) 110, 816
RsaNI GTAC 2 cut(s) 109, 815
RseI CAYNNNNRTG 4 cut(s) 132, 627, 1046, 1172
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 5 cut(s) 650, 717, 732, 777, 791
SatI GCNGC 5 cut(s) 41, 81, 125, 438, 966
Sau3AI GATC 6 cut(s) 181, 295, 446, 695, 807, 835
Sau96I GGNCC 6 cut(s) 30, 52, 203, 227, 269, 1103
SchI GAGTC 4 cut(s) 206, 230, 626, 1061
ScrFI CCNGG 2 cut(s) 191, 887
SduI GDGCHC 4 cut(s) 215, 579, 876, 934
SfaNI GCATC 4 cut(s) 67, 319, 600, 863
Sfr274I CTCGAG 1 cut(s) 218
SinI GGWCC 4 cut(s) 52, 203, 269, 1103
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 4 cut(s) 132, 627, 1046, 1172
SmlI CTYRAG 2 cut(s) 176, 218
SmoI CTYRAG 2 cut(s) 176, 218
Sse9I AATT 6 cut(s) 140, 169, 432, 728, 786, 839
SsiI CCGC 4 cut(s) 267, 830, 924, 1021
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 189, 885
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 3 cut(s) 260, 339, 1048
TaiI ACGT 3 cut(s) 286, 357, 501
TaqI TCGA 3 cut(s) 219, 806, 1070
TasI AATT 6 cut(s) 140, 169, 432, 728, 786, 839
TatI WGTACW 1 cut(s) 814
TfiI GAWTC 2 cut(s) 506, 593
Tru1I TTAA 5 cut(s) 650, 717, 732, 777, 791
Tru9I TTAA 5 cut(s) 650, 717, 732, 777, 791
TscAI CASTG 1 cut(s) 1053
TseI GCWGC 5 cut(s) 40, 80, 124, 437, 965
TspDTI ATGAA 6 cut(s) 32, 146, 437, 548, 834, 1124
TspGWI ACGGA 2 cut(s) 376, 1105
TspRI CASTG 1 cut(s) 1053
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 4 cut(s) 52, 203, 269, 1103
XapI RAATTY 2 cut(s) 140, 728
XbaI TCTAGA 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.