MD05G1124200.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002066
Physical Location & Seq
Forward (+)
24645353 .. 24650302
4950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1124200.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGTTATCAGAGTTGATGAACTTCTTGAGGTCCTGTTTTCGGCCGAGGTCGAATCGATACGTTCATACTGGTGCAGATTCCGGGGGTCGCCAAGACGGGCTCCTGTGGTACAAAGACACAGGCCAGCACATAAATGGTGATTTCTCAATGGCTGTAGTTCAGGCCAACAATTTACTTGAGGATCAGAGTCAGCTCGAGTCGGGTAGTTTGAGCACACATGAATCTGGCGCCTATGGTACTTTTGTTGGTGTTTATGACGGTCATGGGGGTCCGGAGACGTCACGCTATATCAATGATCACCTCTTTCAACATCTCAAGAGATTTTGTTTGGAGCAGCAGTCCATGTCTGTGGATGTAATACGAAAAGCATTTCAAGCAACAGAAGAGGGTTTTCTTTCCGTAGTTACTAGGGAGTGGCCTATGAAACCACAGATTGCAGCAGTAGGATCGTGCTGCCTTGTTGGTGTTATTTGTAATGGAACTCTTTATATCGCTAACTGTGGTGATTCCCGTGCTGTTTTGGGAAGAATAATGAAGGCAACAGGGGAGGTGCTCTCTATTCAGTTGTCACAAGAGCACAATGCCTCTATAGAATCTGTGAGACAGGAGCTGCAATCTTTGCACCCTGATGATCCACATATTGTAGTTTTGAAGCATAATGTATGGCGTGTGAAAGGCTTAATACAGATTTCTAGATCTATTGGTGATGTGTATTTAAAAAAGGCTGAATTTAACAGGGAGCCTTTGTTTTCCAAGTTTCGTCTTCGGGACCCTATCAAAAGACCAATTTTGAGCGCTGATCCGGCAATTTCAGAGATCCAATTGCAGCCACATGATCAATTTATTATATTTGCATCTGATGGGCTCTGGGAACACCTCACCAATCAGGAAGCAGTGGACATAGTTCAAAAACATCCACGAAATGGAAGTGCAAAAAGGCTGATAAAAACCGCCCTGCAAGAAGCAGCAAAAAAGAGAGAGATGAGGTATTCGGACTTGAAGAAGATTGACCGTGGGGTTCGCCGCCATTTCCACGATGACATAACTGTGATCGTCGTGTTTCTTGACTCAAACCTTGTGAGCAAGGCTAGTTCTGTCAAGTGCCCGAACCTATCGGTGAGAGGAGGTGGAATCAACCTGCCTCCTAACACTCTTGCTCCATATGCCACGACAACGGAAGCTGGCAATACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

398

Amino Acids

44.3

Weight (kDa)

8.39

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 324 9.5e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 1146
AccB1I GGYRCC 1 cut(s) 227
AccB7I CCANNNNNTGG 1 cut(s) 923
AccIII TCCGGA 1 cut(s) 271
AciI CCGC 2 cut(s) 951, 1024
AclWI GGATC 5 cut(s) 189, 454, 626, 794, 811
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 728
AcyI GRCGYC 2 cut(s) 228, 278
AfaI GTAC 2 cut(s) 110, 238
AfeI AGCGCT 1 cut(s) 796
AfiI CCNNNNNNNGG 2 cut(s) 39, 923
AgsI TTSAA 5 cut(s) 308, 374, 652, 908, 1000
AluBI AGCT 3 cut(s) 193, 610, 1181
AluI AGCT 3 cut(s) 193, 610, 1181
Alw21I GWGCWC 3 cut(s) 215, 555, 579
Alw26I GTCTC 2 cut(s) 269, 595
AlwI GGATC 5 cut(s) 189, 454, 626, 794, 811
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 1 cut(s) 194
Aor13HI TCCGGA 1 cut(s) 271
Aor51HI AGCGCT 1 cut(s) 796
AoxI GGCC 4 cut(s) 41, 121, 162, 416
ApeKI GCWGC 6 cut(s) 334, 437, 453, 610, 826, 965
ApoI RAATTY 1 cut(s) 728
AspLEI GCGC 2 cut(s) 230, 797
AspS9I GGNCC 3 cut(s) 30, 269, 769
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 6 cut(s) 149, 290, 515, 716, 871, 1129
AvaI CYCGRG 1 cut(s) 194
AvaII GGWCC 3 cut(s) 30, 269, 769
BaeGI GKGCMC 1 cut(s) 1106
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 2 cut(s) 102, 867
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 3 cut(s) 215, 555, 579
BbvI GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
BccI CCATC 1 cut(s) 854
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BclI TGATCA 2 cut(s) 295, 835
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 2 cut(s) 269, 595
BfaI CTAG 3 cut(s) 408, 693, 1089
BfmI CTRYAG 2 cut(s) 153, 588
BfoI RGCGCY 2 cut(s) 231, 798
BfuAI ACCTGC 1 cut(s) 1146
BglII AGATCT 1 cut(s) 695
BisI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
BlsI GCNGC 7 cut(s) 336, 439, 455, 612, 828, 967, 1025
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 3 cut(s) 30, 269, 769
BmeT110I CYCGRG 1 cut(s) 194
BmgT120I GGNCC 3 cut(s) 30, 269, 769
BmiI GGNNCC 6 cut(s) 101, 229, 270, 741, 770, 771
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 863
BpiI GAAGAC 1 cut(s) 755
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpuEI CTTGAG 3 cut(s) 46, 197, 299
BpuMI CCSGG 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 55
BsaHI GRCGYC 2 cut(s) 228, 278
BsaJI CCNNGG 3 cut(s) 44, 81, 1012
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 6 cut(s) 323, 353, 731, 761, 1141, 1171
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 923
Bse1I ACTGG 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 271
BseCI ATCGAT 1 cut(s) 55
BseDI CCNNGG 3 cut(s) 44, 81, 1012
BseGI GGATG 2 cut(s) 358, 913
BseLI CCNNNNNNNGG 2 cut(s) 39, 923
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 1137
BseSI GKGCMC 1 cut(s) 1106
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
BsgI GTGCAG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 4 cut(s) 43, 123, 164, 418
BshNI GGYRCC 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 55
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 3 cut(s) 215, 555, 579
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 3 cut(s) 81, 272, 803
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 2 cut(s) 39, 923
BsmAI GTCTC 2 cut(s) 269, 595
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 782
BsnI GGCC 4 cut(s) 43, 123, 164, 418
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 6 cut(s) 102, 215, 555, 579, 867, 1106
Bsp13I TCCGGA 1 cut(s) 271
Bsp143I GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
BspACI CCGC 2 cut(s) 951, 1024
BspANI GGCC 4 cut(s) 43, 123, 164, 418
BspDI ATCGAT 1 cut(s) 55
BspEI TCCGGA 1 cut(s) 271
BspLI GGNNCC 6 cut(s) 101, 229, 270, 741, 770, 771
BspMI ACCTGC 1 cut(s) 1146
BspPI GGATC 5 cut(s) 189, 454, 626, 794, 811
BspT107I GGYRCC 1 cut(s) 227
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 44, 81, 1012
BssMI GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
BssNI GRCGYC 2 cut(s) 228, 278
Bst4CI ACNGT 4 cut(s) 260, 500, 1013, 1048
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 2 cut(s) 228, 278
BstAPI GCANNNNNTGC 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 125, 1183
BstDSI CCRYGG 1 cut(s) 1012
BstF5I GGATG 2 cut(s) 358, 913
BstH2I RGCGCY 2 cut(s) 231, 798
BstHHI GCGC 2 cut(s) 230, 797
BstKTI GATC 9 cut(s) 184, 298, 449, 634, 698, 802, 819, 838, 1053
BstMAI GTCTC 2 cut(s) 269, 595
BstMBI GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 4 cut(s) 374, 619, 803, 1163
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 2 cut(s) 153, 588
BstSLI GKGCMC 1 cut(s) 1106
BstV1I GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 2 cut(s) 695, 816
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 2 cut(s) 695, 816
BstZI CGGCCG 1 cut(s) 41
Bsu15I ATCGAT 1 cut(s) 55
BsuRI GGCC 4 cut(s) 43, 123, 164, 418
BsuTUI ATCGAT 1 cut(s) 55
BtgI CCRYGG 1 cut(s) 1012
BtsCI GGATG 2 cut(s) 358, 913
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 1 cut(s) 900
BveI ACCTGC 1 cut(s) 1146
Cac8I GCNNGC 2 cut(s) 125, 1183
CaiI CAGNNNCTG 1 cut(s) 610
CfoI GCGC 2 cut(s) 230, 797
Cfr13I GGNCC 3 cut(s) 30, 269, 769
ClaI ATCGAT 1 cut(s) 55
Csp6I GTAC 2 cut(s) 109, 237
CviAII CATG 4 cut(s) 218, 263, 343, 833
CviQI GTAC 2 cut(s) 109, 237
DinI GGCGCC 1 cut(s) 229
DpnI GATC 9 cut(s) 183, 297, 448, 633, 697, 801, 818, 837, 1052
DpnII GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
DraI TTTAAA 1 cut(s) 717
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EclXI CGGCCG 1 cut(s) 41
Eco24I GRGCYC 2 cut(s) 102, 867
Eco47I GGWCC 3 cut(s) 30, 269, 769
Eco47III AGCGCT 1 cut(s) 796
Eco52I CGGCCG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 194
EcoO109I RGGNCCY 2 cut(s) 30, 769
EcoT38I GRGCYC 2 cut(s) 102, 867
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 4 cut(s) 221, 266, 346, 836
FaqI GGGAC 1 cut(s) 782
FatI CATG 4 cut(s) 217, 262, 342, 832
FauNDI CATATG 1 cut(s) 1162
FbaI TGATCA 2 cut(s) 295, 835
Fnu4HI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
FokI GGATG 2 cut(s) 365, 900
FriOI GRGCYC 2 cut(s) 102, 867
Fsp4HI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
FspBI CTAG 3 cut(s) 408, 693, 1089
GlaI GCGC 2 cut(s) 229, 796
GluI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
HaeII RGCGCY 2 cut(s) 231, 798
HaeIII GGCC 4 cut(s) 43, 123, 164, 418
HapII CCGG 3 cut(s) 81, 272, 803
HhaI GCGC 2 cut(s) 230, 797
Hin1I GRCGYC 2 cut(s) 228, 278
Hin1II CATG 4 cut(s) 221, 266, 346, 836
Hin6I GCGC 2 cut(s) 228, 795
HinP1I GCGC 2 cut(s) 228, 795
HinfI GANTC 9 cut(s) 52, 77, 187, 197, 221, 506, 593, 1067, 1131
HpaII CCGG 3 cut(s) 81, 272, 803
HphI GGTGA 6 cut(s) 149, 290, 515, 716, 871, 1129
Hpy166II GTNNAC 1 cut(s) 898
Hpy188I TCNGA 5 cut(s) 10, 186, 814, 859, 994
Hpy188III TCNNGA 7 cut(s) 25, 272, 316, 693, 767, 887, 1064
Hpy8I GTNNAC 1 cut(s) 898
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 4 cut(s) 260, 500, 1013, 1048
HpyCH4IV ACGT 2 cut(s) 60, 278
HpyCH4V TGCA 8 cut(s) 74, 437, 613, 622, 826, 854, 932, 958
HpyF10VI GCNNNNNNNGC 4 cut(s) 374, 619, 803, 1163
HpySE526I ACGT 2 cut(s) 60, 278
Hsp92I GRCGYC 2 cut(s) 228, 278
Hsp92II CATG 4 cut(s) 221, 266, 346, 836
HspAI GCGC 2 cut(s) 228, 795
KasI GGCGCC 1 cut(s) 227
KflI GGGWCCC 1 cut(s) 769
Kpn2I TCCGGA 1 cut(s) 271
Ksp22I TGATCA 2 cut(s) 295, 835
Kzo9I GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
LmnI GCTCC 5 cut(s) 105, 331, 607, 739, 1162
Lsp1109I GCAGC 6 cut(s) 346, 440, 449, 597, 838, 977
LweI GCATC 1 cut(s) 863
MaeI CTAG 3 cut(s) 408, 693, 1089
MaeII ACGT 2 cut(s) 60, 278
MaeIII GTNAC 3 cut(s) 279, 403, 567
MalI GATC 9 cut(s) 183, 297, 448, 633, 697, 801, 818, 837, 1052
MboI GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
MboII GAAGA 5 cut(s) 395, 537, 755, 1012, 1015
MfeI CAATTG 1 cut(s) 821
MflI RGATCY 2 cut(s) 695, 816
MhlI GDGCHC 6 cut(s) 102, 215, 555, 579, 867, 1106
MluCI AATT 6 cut(s) 169, 728, 786, 807, 821, 839
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 3 cut(s) 196, 206, 1061
MroI TCCGGA 1 cut(s) 271
MseI TTAA 3 cut(s) 680, 716, 732
MslI CAYNNNNRTG 5 cut(s) 69, 132, 347, 627, 1046
MspI CCGG 3 cut(s) 81, 272, 803
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 821
MwoI GCNNNNNNNGC 4 cut(s) 374, 619, 803, 1163
NarI GGCGCC 1 cut(s) 228
NciI CCSGG 1 cut(s) 82
NdeI CATATG 1 cut(s) 1162
NdeII GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
NlaIII CATG 4 cut(s) 221, 266, 346, 836
NlaIV GGNNCC 6 cut(s) 101, 229, 270, 741, 770, 771
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 279, 567
PaeR7I CTCGAG 1 cut(s) 194
PfeI GAWTC 6 cut(s) 52, 77, 221, 506, 593, 1131
PflMI CCANNNNNTGG 1 cut(s) 923
PkrI GCNGC 7 cut(s) 336, 439, 455, 612, 828, 967, 1025
PleI GAGTC 3 cut(s) 195, 205, 1061
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 3 cut(s) 195, 205, 1061
PpuMI RGGWCCY 2 cut(s) 30, 769
Psp5II RGGWCCY 2 cut(s) 30, 769
PspN4I GGNNCC 6 cut(s) 101, 229, 270, 741, 770, 771
PspPI GGNCC 3 cut(s) 30, 269, 769
PspPPI RGGWCCY 2 cut(s) 30, 769
PspXI VCTCGAGB 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 2 cut(s) 695, 816
RsaI GTAC 2 cut(s) 110, 238
RsaNI GTAC 2 cut(s) 109, 237
RseI CAYNNNNRTG 5 cut(s) 69, 132, 347, 627, 1046
SaqAI TTAA 3 cut(s) 680, 716, 732
SatI GCNGC 7 cut(s) 335, 438, 454, 611, 827, 966, 1024
Sau3AI GATC 9 cut(s) 181, 295, 446, 631, 695, 799, 816, 835, 1050
Sau96I GGNCC 3 cut(s) 30, 269, 769
SchI GAGTC 3 cut(s) 196, 206, 1061
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 6 cut(s) 102, 215, 555, 579, 867, 1106
SfaNI GCATC 1 cut(s) 863
SfcI CTRYAG 2 cut(s) 153, 588
SfoI GGCGCC 1 cut(s) 229
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 3 cut(s) 30, 269, 769
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 5 cut(s) 69, 132, 347, 627, 1046
SmlI CTYRAG 4 cut(s) 25, 176, 194, 314
SmoI CTYRAG 4 cut(s) 25, 176, 194, 314
Sse9I AATT 6 cut(s) 169, 728, 786, 807, 821, 839
SsiI CCGC 2 cut(s) 951, 1024
SspDI GGCGCC 1 cut(s) 227
SspMI CTAG 3 cut(s) 408, 693, 1089
StyD4I CCNGG 1 cut(s) 80
TaaI ACNGT 4 cut(s) 260, 500, 1013, 1048
TaiI ACGT 2 cut(s) 63, 281
TaqI TCGA 3 cut(s) 50, 55, 195
TasI AATT 6 cut(s) 169, 728, 786, 807, 821, 839
TauI GCSGC 1 cut(s) 1026
TfiI GAWTC 6 cut(s) 52, 77, 221, 506, 593, 1131
Tru1I TTAA 3 cut(s) 680, 716, 732
Tru9I TTAA 3 cut(s) 680, 716, 732
TscAI CASTG 1 cut(s) 900
TseFI GTSAC 2 cut(s) 279, 567
TseI GCWGC 6 cut(s) 334, 437, 453, 610, 826, 965
Tsp45I GTSAC 2 cut(s) 279, 567
TspDTI ATGAA 5 cut(s) 32, 53, 234, 437, 548
TspGWI ACGGA 2 cut(s) 388, 1190
TspRI CASTG 1 cut(s) 900
Van91I CCANNNNNTGG 1 cut(s) 923
VpaK11BI GGWCC 3 cut(s) 30, 269, 769
XapI RAATTY 1 cut(s) 728
XbaI TCTAGA 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XhoI CTCGAG 1 cut(s) 194
XspI CTAG 3 cut(s) 408, 693, 1089
ZraI GACGTC 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.