Rw2G009430

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002066
Physical Location & Seq
Reverse (-)
9465799 .. 9469150
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009430.1

Sequence Viewer

Length: 1182 bp
ATGTTATCAAGATTGATGAACTTTCTGAGGGCCTGCTGGCGACCATCTTCGGACGGATATGTGCATGCAGGTTCCGATGCAGCTGGCAAACAAGAAGGATTACTTTGGTATAAAGACTTTGGGCAACACTTGAATGGTGACTTTTCAATGGCTGTTGTACAGGCCAACAATTTGCTTGAGGATCAGAGCCAGCTTGAGTCTGGTCCCTTGAGCTCGCTTGAGTCTGGCCCCTATGGCACTTTTATTGGAGTTTATGATGGGCACGGCGGCCCTGAGACCTCCCGTTATGTCAATGATCACCTATTCCAGAATTTGAAAAGGTTCACGTCAGAGCAACAGTCCATGTCAGTGGATGTGATACGGAAAGCATATCAAGCAACAGAAGATGGATTCCTCTCTATTGTTACCAAACAATGGCCTGTGAAGCCCCAAATTGCAGCCGTTGGATCTTGTTGCCTTGTTGGTGTGATCTGTGGAGGCACACTTTACATTGCCAACGTTGGTGACTCCCGCGCTGTGCTGGGGAGAGTTGTGAAGGCAACTGGAGAGGTACTTGCCATCCAGCTGTCAGCAGAGCACAATGTAGCGATAGAGTCTGTCAGACGGGAGATGCATTCTTTGCATCCAGACGACTCTCATATTGTAGTTTTGAAGCATAATGTATGGCGTGTAAAGGGATTGATACAGATTTCTAGATCCATTGGAGATGTATATCTAAAAAAGCCCGAGTTTAACAGGGAGCCTTTGTATGCCAAGTTTCGCCTGCGTGAACCTTTCAAAAGGCCAATTTTAAGCGCTGAACCAGCAATTTCTGTGCATGAACTTCAACCACATGATCAATTTCTCATATTTGCTTCGGATGGACTCTGGGAGCATCTTACCAATCAGGAAGCAGTTGATATAGTTCAAAGTCATCCACACAGCGGAAGTGCTCGGAGGCTTGTCAAAGCTGCATTGCTGGAAGCGGCTAAGAAGAGAGAAATGAGGTACTCCGATTTGAAGAAAATAGACCGTGGTGTCCGCAGGCATTTCCACGATGACATAACAGTCATAGTTGTATTTCTTGACTCGAACCTTGTGAGCAGAGCTAGCTCAACCAGGGGCCCTACTTTGTCTGTGAGAGGAGGTGGTATTAATCTACCCGCAAAAACTCTTGCCCCCATGGAACTGAATGCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

393

Amino Acids

43.57

Weight (kDa)

8.56

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 79 - 325 1.1e-39 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1016, 1046
Acc36I ACCTGC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 414
AccII CGCG 1 cut(s) 513
AciI CCGC 6 cut(s) 267, 511, 924, 965, 1021, 1143
AclI AACGTT 1 cut(s) 498
AclWI GGATC 3 cut(s) 189, 454, 690
AcsI RAATTY 1 cut(s) 310
AfaI GTAC 3 cut(s) 159, 552, 989
AfeI AGCGCT 1 cut(s) 796
AfiI CCNNNNNNNGG 2 cut(s) 414, 923
AgsI TTSAA 8 cut(s) 133, 147, 316, 652, 778, 827, 908, 1000
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 1 cut(s) 1097
AluBI AGCT 8 cut(s) 83, 193, 213, 565, 950, 1088, 1092, 1179
AluI AGCT 8 cut(s) 83, 193, 213, 565, 950, 1088, 1092, 1179
Alw21I GWGCWC 3 cut(s) 215, 579, 934
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 189, 454, 690
Ama87I CYCGRG 1 cut(s) 725
Aor51HI AGCGCT 1 cut(s) 796
AoxI GGCC 7 cut(s) 30, 162, 226, 268, 416, 782, 1102
ApaI GGGCCC 1 cut(s) 1106
ApeKI GCWGC 3 cut(s) 80, 437, 950
ApoI RAATTY 1 cut(s) 310
AseI ATTAAT 1 cut(s) 1134
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 2 cut(s) 515, 797
AspS9I GGNCC 6 cut(s) 30, 203, 227, 269, 1102, 1103
AsuHPI GGTGA 3 cut(s) 149, 290, 515
AsuNHI GCTAGC 2 cut(s) 1088, 1175
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 2 cut(s) 264, 1106
BanII GRGCYC 2 cut(s) 215, 1106
Bbv12I GWGCWC 3 cut(s) 215, 579, 934
BbvI GCAGC 3 cut(s) 92, 449, 937
BccI CCATC 5 cut(s) 52, 251, 380, 566, 854
BceAI ACGGC 2 cut(s) 280, 425
BciT130I CCWGG 1 cut(s) 1099
BclI TGATCA 2 cut(s) 295, 835
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 3 cut(s) 693, 1089, 1176
BfoI RGCGCY 1 cut(s) 798
BfuAI ACCTGC 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 5 cut(s) 81, 268, 438, 951, 966
BlsI GCNGC 5 cut(s) 82, 269, 439, 952, 967
Bme1390I CCNGG 1 cut(s) 1099
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 725
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 6 cut(s) 30, 203, 227, 269, 1102, 1103
BmiI GGNNCC 6 cut(s) 73, 205, 229, 741, 1103, 1104
BmrFI CCNGG 1 cut(s) 1099
BmsI GCATC 4 cut(s) 67, 600, 631, 883
BmtI GCTAGC 2 cut(s) 1092, 1179
BpmI CTGGAG 1 cut(s) 564
BpuEI CTTGAG 4 cut(s) 197, 215, 229, 239
BsaBI GATNNNNATC 1 cut(s) 711
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 1012, 1098, 1161
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 923
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 2 cut(s) 489, 953
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 1 cut(s) 1099
BseDI CCNNGG 3 cut(s) 1012, 1098, 1161
BseGI GGATG 5 cut(s) 358, 558, 622, 865, 913
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 2 cut(s) 414, 923
BseMI GCAATG 2 cut(s) 489, 953
BseMII CTCAG 2 cut(s) 17, 264
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 1137
BseSI GKGCMC 2 cut(s) 264, 1106
BseXI GCAGC 3 cut(s) 92, 449, 937
BseYI CCCAGC 1 cut(s) 520
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 7 cut(s) 32, 164, 228, 270, 418, 784, 1104
BsiHKAI GWGCWC 3 cut(s) 215, 579, 934
BsiHKCI CYCGRG 1 cut(s) 725
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 2 cut(s) 414, 923
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 189
BsmI GAATGC 2 cut(s) 613, 1177
BsnI GGCC 7 cut(s) 32, 164, 228, 270, 418, 784, 1104
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 725
Bsp120I GGGCCC 1 cut(s) 1102
Bsp1286I GDGCHC 5 cut(s) 215, 264, 579, 934, 1106
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 6 cut(s) 181, 295, 446, 468, 695, 835
Bsp19I CCATGG 1 cut(s) 1161
BspACI CCGC 6 cut(s) 267, 511, 924, 965, 1021, 1143
BspANI GGCC 7 cut(s) 32, 164, 228, 270, 418, 784, 1104
BspCNI CTCAG 2 cut(s) 18, 265
BspFNI CGCG 1 cut(s) 513
BspLI GGNNCC 6 cut(s) 73, 205, 229, 741, 1103, 1104
BspMI ACCTGC 1 cut(s) 59
BspOI GCTAGC 2 cut(s) 1092, 1179
BspPI GGATC 3 cut(s) 189, 454, 690
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 2 cut(s) 489, 953
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 1012, 1098, 1161
BssMI GATC 6 cut(s) 181, 295, 446, 468, 695, 835
BssT1I CCWWGG 1 cut(s) 1161
Bst2UI CCWGG 1 cut(s) 1099
Bst4CI ACNGT 3 cut(s) 339, 1013, 1048
Bst6I CTCTTC 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 619
BstAUI TGTACA 1 cut(s) 157
BstDEI CTNAG 3 cut(s) 26, 273, 969
BstDSI CCRYGG 2 cut(s) 1012, 1161
BstF5I GGATG 5 cut(s) 358, 558, 622, 865, 913
BstFNI CGCG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 798
BstHHI GCGC 2 cut(s) 515, 797
BstKTI GATC 6 cut(s) 184, 298, 449, 471, 698, 838
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 6 cut(s) 181, 295, 446, 468, 695, 835
BstMWI GCNNNNNNNGC 6 cut(s) 234, 374, 424, 619, 803, 1089
BstNI CCWGG 1 cut(s) 1099
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 1097
BstSLI GKGCMC 2 cut(s) 264, 1106
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 3 cut(s) 92, 449, 937
BstX2I RGATCY 2 cut(s) 446, 695
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 2 cut(s) 446, 695
BsuRI GGCC 7 cut(s) 32, 164, 228, 270, 418, 784, 1104
BtgI CCRYGG 2 cut(s) 1012, 1161
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 5 cut(s) 358, 558, 622, 865, 913
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 59
CfoI GCGC 2 cut(s) 515, 797
Cfr13I GGNCC 6 cut(s) 30, 203, 227, 269, 1102, 1103
Csp6I GTAC 3 cut(s) 158, 551, 988
CviAII CATG 5 cut(s) 65, 343, 818, 833, 1162
CviQI GTAC 3 cut(s) 158, 551, 988
DdeI CTNAG 3 cut(s) 26, 273, 969
DpnI GATC 6 cut(s) 183, 297, 448, 470, 697, 837
DpnII GATC 6 cut(s) 181, 295, 446, 468, 695, 835
DrdI GACNNNNNNGTC 2 cut(s) 1016, 1046
DseDI GACNNNNNNGTC 2 cut(s) 1016, 1046
Eam1104I CTCTTC 1 cut(s) 968
EarI CTCTTC 1 cut(s) 968
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 1161
Eco24I GRGCYC 2 cut(s) 215, 1106
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 203
Eco47III AGCGCT 1 cut(s) 796
Eco53kI GAGCTC 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 213
EcoO109I RGGNCCY 3 cut(s) 30, 1102, 1103
EcoRII CCWGG 1 cut(s) 1097
EcoT14I CCWWGG 1 cut(s) 1161
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 2 cut(s) 215, 1106
ErhI CCWWGG 1 cut(s) 1161
FaeI CATG 5 cut(s) 68, 346, 821, 836, 1165
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 1 cut(s) 189
FatI CATG 5 cut(s) 64, 342, 817, 832, 1161
FauI CCCGC 2 cut(s) 518, 1150
FbaI TGATCA 2 cut(s) 295, 835
Fnu4HI GCNGC 5 cut(s) 81, 268, 438, 951, 966
FokI GGATG 5 cut(s) 365, 545, 609, 872, 900
FriOI GRGCYC 2 cut(s) 215, 1106
Fsp4HI GCNGC 5 cut(s) 81, 268, 438, 951, 966
FspBI CTAG 3 cut(s) 693, 1089, 1176
GlaI GCGC 2 cut(s) 514, 796
GluI GCNGC 5 cut(s) 81, 268, 438, 951, 966
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 798
HaeIII GGCC 7 cut(s) 32, 164, 228, 270, 418, 784, 1104
HhaI GCGC 2 cut(s) 515, 797
Hin1II CATG 5 cut(s) 68, 346, 821, 836, 1165
Hin6I GCGC 2 cut(s) 513, 795
HinP1I GCGC 2 cut(s) 513, 795
HinfI GANTC 8 cut(s) 197, 221, 390, 506, 593, 632, 864, 1067
HphI GGTGA 3 cut(s) 149, 290, 515
Hpy166II GTNNAC 2 cut(s) 324, 770
Hpy188I TCNGA 9 cut(s) 27, 52, 76, 186, 331, 602, 859, 936, 994
Hpy188III TCNNGA 6 cut(s) 9, 307, 626, 693, 887, 1064
Hpy8I GTNNAC 2 cut(s) 324, 770
HpyAV CCTTC 2 cut(s) 89, 529
HpyCH4III ACNGT 3 cut(s) 339, 1013, 1048
HpyCH4IV ACGT 2 cut(s) 326, 498
HpyCH4V TGCA 8 cut(s) 64, 68, 80, 437, 613, 622, 817, 953
HpyF10VI GCNNNNNNNGC 6 cut(s) 234, 374, 424, 619, 803, 1089
HpyF3I CTNAG 3 cut(s) 26, 273, 969
HpySE526I ACGT 2 cut(s) 326, 498
Hsp92II CATG 5 cut(s) 68, 346, 821, 836, 1165
HspAI GCGC 2 cut(s) 513, 795
Ksp22I TGATCA 2 cut(s) 295, 835
Kzo9I GATC 6 cut(s) 181, 295, 446, 468, 695, 835
LmnI GCTCC 2 cut(s) 739, 871
Lsp1109I GCAGC 3 cut(s) 92, 449, 937
LweI GCATC 4 cut(s) 67, 600, 631, 883
MaeI CTAG 3 cut(s) 693, 1089, 1176
MaeII ACGT 2 cut(s) 326, 498
MaeIII GTNAC 3 cut(s) 137, 403, 503
MalI GATC 6 cut(s) 183, 297, 448, 470, 697, 837
MboI GATC 6 cut(s) 181, 295, 446, 468, 695, 835
MboII GAAGA 4 cut(s) 39, 395, 985, 1012
MflI RGATCY 2 cut(s) 446, 695
MhlI GDGCHC 5 cut(s) 215, 264, 579, 934, 1106
MluCI AATT 6 cut(s) 169, 310, 432, 786, 807, 839
MlyI GAGTC 7 cut(s) 206, 230, 500, 602, 626, 858, 1061
MmeI TCCRAC 1 cut(s) 424
Mph1103I ATGCAT 1 cut(s) 615
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 3 cut(s) 732, 791, 1134
MslI CAYNNNNRTG 2 cut(s) 132, 347
MspA1I CMGCKG 3 cut(s) 83, 565, 924
MspR9I CCNGG 1 cut(s) 1099
Mva1269I GAATGC 2 cut(s) 613, 1177
MvaI CCWGG 1 cut(s) 1099
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 6 cut(s) 234, 374, 424, 619, 803, 1089
NcoI CCATGG 1 cut(s) 1161
NdeII GATC 6 cut(s) 181, 295, 446, 468, 695, 835
NheI GCTAGC 2 cut(s) 1088, 1175
NlaIII CATG 5 cut(s) 68, 346, 821, 836, 1165
NlaIV GGNNCC 6 cut(s) 73, 205, 229, 741, 1103, 1104
NmuCI GTSAC 2 cut(s) 137, 503
NsiI ATGCAT 1 cut(s) 615
NspI RCATGY 1 cut(s) 68
PaeI GCATGC 1 cut(s) 68
PctI GAATGC 2 cut(s) 613, 1177
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 390
PflMI CCANNNNNTGG 1 cut(s) 414
PkrI GCNGC 5 cut(s) 82, 269, 439, 952, 967
PleI GAGTC 7 cut(s) 205, 229, 500, 601, 626, 858, 1061
PpsI GAGTC 7 cut(s) 205, 229, 500, 601, 626, 858, 1061
PshBI ATTAAT 1 cut(s) 1134
Psp124BI GAGCTC 1 cut(s) 215
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 1 cut(s) 1097
PspN4I GGNNCC 6 cut(s) 73, 205, 229, 741, 1103, 1104
PspOMI GGGCCC 1 cut(s) 1102
PspPI GGNCC 6 cut(s) 30, 203, 227, 269, 1102, 1103
PsuI RGATCY 2 cut(s) 446, 695
PvuII CAGCTG 2 cut(s) 83, 565
RsaI GTAC 3 cut(s) 159, 552, 989
RsaNI GTAC 3 cut(s) 158, 551, 988
RseI CAYNNNNRTG 2 cut(s) 132, 347
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 3 cut(s) 732, 791, 1134
SatI GCNGC 5 cut(s) 81, 268, 438, 951, 966
Sau3AI GATC 6 cut(s) 181, 295, 446, 468, 695, 835
Sau96I GGNCC 6 cut(s) 30, 203, 227, 269, 1102, 1103
SchI GAGTC 7 cut(s) 206, 230, 500, 602, 626, 858, 1061
ScrFI CCNGG 1 cut(s) 1099
SduI GDGCHC 5 cut(s) 215, 264, 579, 934, 1106
SfaNI GCATC 4 cut(s) 67, 600, 631, 883
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 132, 347
SmlI CTYRAG 4 cut(s) 176, 194, 208, 218
SmoI CTYRAG 4 cut(s) 176, 194, 208, 218
SphI GCATGC 1 cut(s) 68
Sse9I AATT 6 cut(s) 169, 310, 432, 786, 807, 839
SsiI CCGC 6 cut(s) 267, 511, 924, 965, 1021, 1143
SspMI CTAG 3 cut(s) 693, 1089, 1176
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 1097
StyI CCWWGG 1 cut(s) 1161
TaaI ACNGT 3 cut(s) 339, 1013, 1048
TaiI ACGT 2 cut(s) 329, 501
TaqI TCGA 1 cut(s) 1070
TasI AATT 6 cut(s) 169, 310, 432, 786, 807, 839
TatI WGTACW 1 cut(s) 157
TauI GCSGC 2 cut(s) 270, 968
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 3 cut(s) 732, 791, 1134
Tru9I TTAA 3 cut(s) 732, 791, 1134
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 2 cut(s) 137, 503
TseI GCWGC 3 cut(s) 80, 437, 950
Tsp45I GTSAC 2 cut(s) 137, 503
TspDTI ATGAA 2 cut(s) 32, 834
TspGWI ACGGA 2 cut(s) 69, 376
TspRI CASTG 1 cut(s) 354
Van91I CCANNNNNTGG 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 203
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 1 cut(s) 310
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 3 cut(s) 693, 1089, 1176
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.