AT5G66750

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003343
Physical Location & Seq
Forward (+)
26648897 .. 26653191
4295 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66750.1

Sequence Viewer

Length: 2295 bp
ATGGTTAGTCTGCGCTCCAGAAAAGTTATTCCGGCTTCGGAAATGGTCAGCGACGGGAAAACGGAGAAAGATGCGTCTGGTGATTCACCCACTTCTGTTCTCAACGAAGAGGAAAACTGTGAGGAGAAAAGTGTTACTGTTGTAGAGGAAGAGATACTTCTAGCCAAAAATGGAGATTCTTCTCTTATTTCTGAAGCCATGGCTCAGGAGGAAGAGCAGCTGCTCAAACTTCGGGAAGATGAAGAGAAAGCTAACAATGCTGGATCTGCTGTTGCTCCTAATCTGAATGAAACTCAGTTTACTAAACTTGATGAGCTCTTGACGCAAACTCAGCTCTACTCTGAGTTTCTCCTTGAGAAAATGGAGGATATCACAATTAATGGGATAGAAAGTGAGAGCCAAAAAGCTGAGCCCGAGAAGACTGGTCGTGGACGCAAAAGAAAGGCTGCTTCTCAGTACAACAATACTAAGGCTAAGAGAGCGGTTGCTGCTATGATTTCAAGATCTAAAGAAGATGGTGAGACCATCAACTCAGATCTGACAGAGGAAGAAACAGTCATCAAACTGCAGAATGAACTTTGTCCTCTTCTCACTGGTGGACAGTTAAAGTCTTATCAGCTTAAAGGTGTCAAATGGCTAATATCATTGTGGCAGAATGGTTTGAATGGAATATTAGCTGATCAAATGGGACTTGGAAAGACGATTCAAACGATCGGTTTCTTATCACATCTGAAAGGGAATGGGTTGGATGGTCCATATCTAGTCATTGCTCCACTGTCTACACTTTCAAATTGGTTCAATGAGATTGCTAGGTTCACGCCTTCCATCAATGCAATCATCTACCATGGGGATAAAAATCAAAGGGATGAGCTCAGGAGGAAGCACATGCCTAAAACTGTTGGTCCCAAGTTCCCTATAGTTATTACTTCTTATGAGGTTGCCATGAATGATGCTAAAAGAATTCTGCGGCACTATCCATGGAAATATGTTGTGATTGATGAGGGCCACAGGTTGAAAAACCACAAGTGTAAATTGTTGAGGGAACTAAAACACTTGAAGATGGATAACAAACTTCTGCTGACAGGAACACCTCTGCAAAATAATCTTTCTGAGCTTTGGTCTTTGTTAAATTTTATTCTGCCTGACATCTTTACATCACATGATGAATTTGAATCATGGTTTGATTTTTCTGAAAAGAACAAAAACGAAGCAACCAAGGAAGAAGAAGAGAAAAGAAGAGCTCAAGTTGTTTCCAAACTTCATGGTATACTACGACCATTCATCCTTCGAAGAATGAAATGTGATGTTGAGCTCTCACTTCCACGGAAAAAGGAGATTATAATGTATGCTACAATGACTGATCATCAGAAAAAGTTCCAGGAACATCTGGTGAATAACACGTTGGAAGCACATCTTGGAGAGAATGCCATCCGAGGTCAAGGCTGGAAGGGAAAGCTTAACAACCTGGTCATTCAACTTCGAAAGAACTGCAACCATCCTGACCTTCTCCAGGGGCAAATAGATGGTTCATATCTCTACCCTCCTGTTGAAGAGATTGTTGGACAGTGTGGTAAATTCCGCTTATTGGAGAGATTACTTGTTCGGTTATTTGCCAATAATCACAAAGTCCTTATCTTCTCCCAATGGACGAAACTTTTGGACATTATGGATTACTACTTCAGTGAGAAGGGGTTTGAGGTTTGCAGAATCGATGGCAGTGTGAAGCTGGATGAAAGGAGAAGACAGATTAAAGATTTCAGTGATGAGAAGAGCAGCTGTAGTATATTTCTCCTGAGTACCAGAGCTGGAGGACTCGGAATCAATCTTACTGCTGCTGATACATGCATCCTCTATGACAGCGACTGGAACCCTCAAATGGACTTGCAAGCCATGGACAGATGCCACAGAATCGGGCAGACGAAACCTGTTCATGTTTATAGGCTTTCCACGGCTCAGTCGATAGAGACCCGGGTTCTGAAACGAGCGTACAGTAAGCTCAAGCTGGAACATGTGGTTATTGGCCAAGGGCAGTTTCATCAAGAACGTGCCAAGTCTTCAACACCTTTAGAGGAAGAGGACATACTGGCGTTGCTTAAGGAAGATGAAACTGCTGAAGATAAGTTGATACAAACCGATATAAGCGATGCGGATCTTGACAGGTTACTTGACCGGAGTGACCTGACAATTACTGCACCGGGAGAGACACAAGCTGCTGAAGCTTTTCCAGTGAAGGGTCCAGGTTGGGAAGTGGTCCTGCCTAGTTCGGGAGGAATGCTGTCTTCCCTGAACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

764

Amino Acids

86.64

Weight (kDa)

6.01

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 201 - 364 2e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 205 - 502 5.1e-86 SNF2-related domain
DEAD PF00270 219 - 368 1.2e-06 DEAD/DEAH box helicase
Helicase_C PF00271 525 - 638 3.9e-27 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
AccBSI CCGCTC 1 cut(s) 482
AccI GTMKAC 2 cut(s) 779, 1267
AciI CCGC 4 cut(s) 482, 967, 1579, 2147
AclWI GGATC 2 cut(s) 271, 2157
AcoI YGGCCR 1 cut(s) 2020
AcsI RAATTY 4 cut(s) 960, 1129, 1166, 1574
AcuI CTGAAG 4 cut(s) 213, 1663, 2133, 2235
AfaI GTAC 3 cut(s) 458, 1798, 1988
AfiI CCNNNNNNNGG 5 cut(s) 1510, 1585, 1876, 2231, 2264
AflII CTTAAG 1 cut(s) 2093
AflIII ACRYGT 2 cut(s) 1398, 2008
AjnI CCWGG 4 cut(s) 1377, 1464, 1509, 2236
AjuI GAANNNNNNNTTGG 8 cut(s) 1385, 1398, 1417, 1430, 1542, 1574, 2016, 2048
Alw21I GWGCWC 4 cut(s) 318, 873, 1243, 1314
Alw26I GTCTC 3 cut(s) 515, 1958, 2195
AlwI GGATC 2 cut(s) 271, 2157
AlwNI CAGNNNCTG 1 cut(s) 1863
Ama87I CYCGRG 2 cut(s) 413, 1968
AoxI GGCC 2 cut(s) 1003, 2020
ApeKI GCWGC 7 cut(s) 217, 220, 446, 488, 1773, 1832, 2210
ApoI RAATTY 4 cut(s) 960, 1129, 1166, 1574
ArsI GACNNNNNNTTYG 2 cut(s) 1639, 1671
AseI ATTAAT 1 cut(s) 378
Asp700I GAANNNNTTC 2 cut(s) 1373, 2220
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 5 cut(s) 752, 902, 1003, 2234, 2251
AsuC2I CCSGG 3 cut(s) 1969, 1970, 2196
AsuHPI GGTGA 4 cut(s) 78, 92, 530, 1402
AsuII TTCGAA 2 cut(s) 1288, 1480
AvaI CYCGRG 2 cut(s) 413, 1968
AvaII GGWCC 4 cut(s) 752, 902, 2234, 2251
BalI TGGCCA 1 cut(s) 2022
BanII GRGCYC 5 cut(s) 318, 414, 873, 1243, 1314
BarI GAAGNNNNNNTAC 2 cut(s) 2064, 2096
BbsI GAAGAC 4 cut(s) 425, 1747, 2046, 2271
Bbv12I GWGCWC 4 cut(s) 318, 873, 1243, 1314
BbvI GCAGC 7 cut(s) 207, 229, 433, 475, 1785, 1819, 2197
BccI CCATC 9 cut(s) 509, 533, 743, 833, 1054, 1436, 1503, 1517, 1706
BceAI ACGGC 1 cut(s) 1965
BcgI CGANNNNNNTGC 2 cut(s) 1470, 1504
BciT130I CCWGG 4 cut(s) 1379, 1466, 1511, 2238
BclI TGATCA 2 cut(s) 679, 1360
BcnI CCSGG 3 cut(s) 1969, 1970, 2196
BcoDI GTCTC 3 cut(s) 515, 1958, 2195
BfaI CTAG 4 cut(s) 161, 761, 810, 2259
BfmI CTRYAG 3 cut(s) 566, 915, 1777
BfrI CTTAAG 1 cut(s) 2093
BglII AGATCT 2 cut(s) 503, 535
BisI GCNGC 8 cut(s) 218, 221, 447, 489, 968, 1774, 1833, 2211
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 8 cut(s) 219, 222, 448, 490, 969, 1775, 1834, 2212
Bme1390I CCNGG 7 cut(s) 1379, 1466, 1511, 1969, 1970, 2196, 2238
Bme18I GGWCC 4 cut(s) 752, 902, 2234, 2251
BmeT110I CYCGRG 2 cut(s) 413, 1968
BmgT120I GGNCC 5 cut(s) 752, 902, 1003, 2234, 2251
BmiI GGNNCC 3 cut(s) 904, 1868, 2235
BmrFI CCNGG 7 cut(s) 1379, 1466, 1511, 1969, 1970, 2196, 2238
BmsI GCATC 5 cut(s) 61, 940, 1854, 1889, 2134
BpiI GAAGAC 4 cut(s) 425, 1747, 2046, 2271
BpmI CTGGAG 2 cut(s) 1493, 1827
Bpu10I CCTNAGC 2 cut(s) 204, 872
Bpu1102I GCTNAGC 1 cut(s) 408
Bpu14I TTCGAA 2 cut(s) 1288, 1480
BpuEI CTTGAG 3 cut(s) 374, 1227, 1982
BpuMI CCSGG 3 cut(s) 1969, 1970, 2196
Bsa29I ATCGAT 1 cut(s) 1710
BsaBI GATNNNNATC 2 cut(s) 855, 2148
BsaI GGTCTC 2 cut(s) 515, 1958
BsaWI WCCGGW 1 cut(s) 2169
Bsc4I CCNNNNNNNGG 5 cut(s) 1510, 1585, 1876, 2231, 2264
Bse1I ACTGG 5 cut(s) 427, 598, 1868, 2088, 2225
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 2 cut(s) 855, 2148
BseBI CCWGG 4 cut(s) 1379, 1466, 1511, 2238
BseCI ATCGAT 1 cut(s) 1710
BseGI GGATG 7 cut(s) 754, 871, 1281, 1428, 1495, 1735, 1845
BseJI GATNNNNATC 2 cut(s) 855, 2148
BseLI CCNNNNNNNGG 5 cut(s) 1510, 1585, 1876, 2231, 2264
BseMI GCAATG 1 cut(s) 765
BseNI ACTGG 5 cut(s) 427, 598, 1868, 2088, 2225
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 7 cut(s) 207, 229, 433, 475, 1785, 1819, 2197
BsgI GTGCAG 1 cut(s) 2175
Bsh1285I CGRYCG 1 cut(s) 714
BshFI GGCC 2 cut(s) 1005, 2022
BshVI ATCGAT 1 cut(s) 1710
BsiEI CGRYCG 1 cut(s) 714
BsiHKAI GWGCWC 4 cut(s) 318, 873, 1243, 1314
BsiHKCI CYCGRG 2 cut(s) 413, 1968
BsiSI CCGG 4 cut(s) 32, 1969, 2170, 2195
BslFI GGGAC 2 cut(s) 702, 888
BslI CCNNNNNNNGG 5 cut(s) 1510, 1585, 1876, 2231, 2264
BsmAI GTCTC 3 cut(s) 515, 1958, 2195
BsmFI GGGAC 2 cut(s) 702, 888
BsmI GAATGC 2 cut(s) 1429, 2277
BsnI GGCC 2 cut(s) 1005, 2022
Bso31I GGTCTC 2 cut(s) 515, 1958
BsoBI CYCGRG 2 cut(s) 413, 1968
Bsp119I TTCGAA 2 cut(s) 1288, 1480
Bsp1286I GDGCHC 5 cut(s) 318, 414, 873, 1243, 1314
Bsp143I GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 4 cut(s) 198, 844, 977, 1890
BspACI CCGC 4 cut(s) 482, 967, 1579, 2147
BspANI GGCC 2 cut(s) 1005, 2022
BspDI ATCGAT 1 cut(s) 1710
BspLI GGNNCC 3 cut(s) 904, 1868, 2235
BspMAI CTGCAG 1 cut(s) 570
BspPI GGATC 2 cut(s) 271, 2157
BspQI GCTCTTC 3 cut(s) 207, 1231, 1763
BspT104I TTCGAA 2 cut(s) 1288, 1480
BspTI CTTAAG 1 cut(s) 2093
BspTNI GGTCTC 2 cut(s) 515, 1958
BsrBI CCGCTC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 765
BsrI ACTGG 5 cut(s) 427, 598, 1868, 2088, 2225
BssMI GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
BssNAI GTATAC 1 cut(s) 1268
BssT1I CCWWGG 6 cut(s) 198, 844, 977, 1215, 1890, 2023
Bst1107I GTATAC 1 cut(s) 1268
Bst2UI CCWGG 4 cut(s) 1379, 1466, 1511, 2238
Bst4CI ACNGT 9 cut(s) 119, 139, 556, 603, 777, 898, 1566, 1991, 2291
BstAFI CTTAAG 1 cut(s) 2093
BstBI TTCGAA 2 cut(s) 1288, 1480
BstC8I GCNNGC 1 cut(s) 1887
BstDSI CCRYGG 6 cut(s) 198, 844, 977, 1322, 1890, 1947
BstENI CCTNNNNNAGG 1 cut(s) 1508
BstF5I GGATG 7 cut(s) 754, 871, 1281, 1428, 1495, 1735, 1845
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 7 cut(s) 266, 506, 538, 682, 714, 1363, 2152
BstMAI GTCTC 3 cut(s) 515, 1958, 2195
BstMBI GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
BstMCI CGRYCG 1 cut(s) 714
BstMWI GCNNNNNNNGC 7 cut(s) 257, 266, 322, 331, 479, 488, 2216
BstNI CCWGG 4 cut(s) 1379, 1466, 1511, 2238
BstNSI RCATGY 3 cut(s) 889, 1845, 2012
BstSCI CCNGG 7 cut(s) 1377, 1464, 1509, 1967, 1968, 2194, 2236
BstSFI CTRYAG 3 cut(s) 566, 915, 1777
BstV1I GCAGC 7 cut(s) 207, 229, 433, 475, 1785, 1819, 2197
BstV2I GAAGAC 4 cut(s) 425, 1747, 2046, 2271
BstX2I RGATCY 4 cut(s) 263, 503, 535, 2149
BstYI RGATCY 4 cut(s) 263, 503, 535, 2149
BstZ17I GTATAC 1 cut(s) 1268
Bsu15I ATCGAT 1 cut(s) 1710
BsuRI GGCC 2 cut(s) 1005, 2022
BsuTUI ATCGAT 1 cut(s) 1710
BtgI CCRYGG 6 cut(s) 198, 844, 977, 1322, 1890, 1947
BtgZI GCGATG 1 cut(s) 2157
BtsCI GGATG 7 cut(s) 754, 871, 1281, 1428, 1495, 1735, 1845
BtsI GCAGTG 1 cut(s) 1723
BtsIMutI CAGTG 7 cut(s) 591, 773, 1571, 1687, 1723, 1765, 2232
Cac8I GCNNGC 1 cut(s) 1887
CaiI CAGNNNCTG 1 cut(s) 1863
CfoI GCGC 1 cut(s) 15
Cfr13I GGNCC 5 cut(s) 752, 902, 1003, 2234, 2251
Cfr9I CCCGGG 1 cut(s) 1968
ClaI ATCGAT 1 cut(s) 1710
CseI GACGC 3 cut(s) 63, 331, 441
CsiI ACCWGGT 1 cut(s) 1464
Csp6I GTAC 3 cut(s) 457, 1797, 1987
CviQI GTAC 3 cut(s) 457, 1797, 1987
DpnI GATC 7 cut(s) 265, 505, 537, 681, 713, 1362, 2151
DpnII GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
EaeI YGGCCR 1 cut(s) 2020
Ecl136II GAGCTC 4 cut(s) 316, 871, 1241, 1312
Eco130I CCWWGG 6 cut(s) 198, 844, 977, 1215, 1890, 2023
Eco24I GRGCYC 5 cut(s) 318, 414, 873, 1243, 1314
Eco31I GGTCTC 2 cut(s) 515, 1958
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 4 cut(s) 752, 902, 2234, 2251
Eco53kI GAGCTC 4 cut(s) 316, 871, 1241, 1312
Eco57I CTGAAG 4 cut(s) 213, 1663, 2133, 2235
Eco88I CYCGRG 2 cut(s) 413, 1968
EcoICRI GAGCTC 4 cut(s) 316, 871, 1241, 1312
EcoNI CCTNNNNNAGG 1 cut(s) 1508
EcoRI GAATTC 1 cut(s) 960
EcoRII CCWGG 4 cut(s) 1377, 1464, 1509, 2236
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 6 cut(s) 198, 844, 977, 1215, 1890, 2023
EcoT22I ATGCAT 1 cut(s) 1847
EcoT38I GRGCYC 5 cut(s) 318, 414, 873, 1243, 1314
ErhI CCWWGG 6 cut(s) 198, 844, 977, 1215, 1890, 2023
FalI AAGNNNNNCTT 4 cut(s) 141, 173, 1398, 1430
FaqI GGGAC 2 cut(s) 702, 888
FbaI TGATCA 2 cut(s) 679, 1360
FblI GTMKAC 2 cut(s) 779, 1267
Fnu4HI GCNGC 8 cut(s) 218, 221, 447, 489, 968, 1774, 1833, 2211
FokI GGATG 7 cut(s) 761, 878, 1268, 1415, 1482, 1742, 1832
FriOI GRGCYC 5 cut(s) 318, 414, 873, 1243, 1314
Fsp4HI GCNGC 8 cut(s) 218, 221, 447, 489, 968, 1774, 1833, 2211
FspBI CTAG 4 cut(s) 161, 761, 810, 2259
GlaI GCGC 1 cut(s) 14
GluI GCNGC 8 cut(s) 218, 221, 447, 489, 968, 1774, 1833, 2211
GsuI CTGGAG 2 cut(s) 1493, 1827
HaeIII GGCC 2 cut(s) 1005, 2022
HapII CCGG 4 cut(s) 32, 1969, 2170, 2195
HgaI GACGC 3 cut(s) 63, 331, 441
HhaI GCGC 1 cut(s) 15
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HindIII AAGCTT 2 cut(s) 1454, 2217
HinfI GANTC 8 cut(s) 83, 176, 703, 1172, 1707, 1812, 1818, 1908
HpaII CCGG 4 cut(s) 32, 1969, 2170, 2195
HphI GGTGA 4 cut(s) 78, 92, 530, 1402
Hpy166II GTNNAC 6 cut(s) 300, 431, 599, 780, 816, 1268
Hpy8I GTNNAC 6 cut(s) 300, 431, 599, 780, 816, 1268
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 6 cut(s) 831, 1295, 1441, 1514, 1681, 2224
HpyCH4III ACNGT 9 cut(s) 119, 139, 556, 603, 777, 898, 1566, 1991, 2291
HpyCH4IV ACGT 2 cut(s) 1400, 2044
HpyCH4V TGCA 8 cut(s) 568, 833, 1096, 1491, 1704, 1845, 1885, 2192
HpyF10VI GCNNNNNNNGC 7 cut(s) 257, 266, 322, 331, 479, 488, 2216
HpySE526I ACGT 2 cut(s) 1400, 2044
HspAI GCGC 1 cut(s) 13
Ksp22I TGATCA 2 cut(s) 679, 1360
Kzo9I GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
LguI GCTCTTC 3 cut(s) 207, 1231, 1763
LmnI GCTCC 3 cut(s) 20, 280, 775
Lsp1109I GCAGC 7 cut(s) 207, 229, 433, 475, 1785, 1819, 2197
LweI GCATC 5 cut(s) 61, 940, 1854, 1889, 2134
MabI ACCWGGT 1 cut(s) 1464
MaeI CTAG 4 cut(s) 161, 761, 810, 2259
MaeII ACGT 2 cut(s) 1400, 2044
MaeIII GTNAC 3 cut(s) 133, 2160, 2174
MalI GATC 7 cut(s) 265, 505, 537, 681, 713, 1362, 2151
MbiI CCGCTC 1 cut(s) 482
MboI GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
MflI RGATCY 4 cut(s) 263, 503, 535, 2149
MhlI GDGCHC 5 cut(s) 318, 414, 873, 1243, 1314
MlsI TGGCCA 1 cut(s) 2022
MluCI AATT 8 cut(s) 375, 790, 960, 1031, 1129, 1166, 1574, 2184
MluNI TGGCCA 1 cut(s) 2022
MlyI GAGTC 1 cut(s) 1806
MmeI TCCRAC 3 cut(s) 726, 1383, 1540
Mox20I TGGCCA 1 cut(s) 2022
Mph1103I ATGCAT 1 cut(s) 1847
MroXI GAANNNNTTC 2 cut(s) 1373, 2220
MscI TGGCCA 1 cut(s) 2022
MseI TTAA 7 cut(s) 378, 605, 621, 1127, 1458, 1749, 2094
Msp20I TGGCCA 1 cut(s) 2022
MspA1I CMGCKG 2 cut(s) 220, 1776
MspCI CTTAAG 1 cut(s) 2093
MspI CCGG 4 cut(s) 32, 1969, 2170, 2195
MspR9I CCNGG 7 cut(s) 1379, 1466, 1511, 1969, 1970, 2196, 2238
Mva1269I GAATGC 2 cut(s) 1429, 2277
MvaI CCWGG 4 cut(s) 1379, 1466, 1511, 2238
MwoI GCNNNNNNNGC 7 cut(s) 257, 266, 322, 331, 479, 488, 2216
NciI CCSGG 3 cut(s) 1969, 1970, 2196
NcoI CCATGG 4 cut(s) 198, 844, 977, 1890
NdeII GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
NlaIV GGNNCC 3 cut(s) 904, 1868, 2235
NmuCI GTSAC 1 cut(s) 2174
NsiI ATGCAT 1 cut(s) 1847
NspI RCATGY 3 cut(s) 889, 1845, 2012
NspV TTCGAA 2 cut(s) 1288, 1480
PciI ACATGT 1 cut(s) 2008
PciSI GCTCTTC 3 cut(s) 207, 1231, 1763
PcsI WCGNNNNNNNCGW 2 cut(s) 707, 1955
PctI GAATGC 2 cut(s) 1429, 2277
PdmI GAANNNNTTC 2 cut(s) 1373, 2220
PfeI GAWTC 7 cut(s) 83, 176, 703, 1172, 1707, 1818, 1908
PfoI TCCNGGA 1 cut(s) 1377
PkrI GCNGC 8 cut(s) 219, 222, 448, 490, 969, 1775, 1834, 2212
Ple19I CGATCG 1 cut(s) 714
PleI GAGTC 1 cut(s) 1806
PpsI GAGTC 1 cut(s) 1806
PscI ACATGT 1 cut(s) 2008
PshBI ATTAAT 1 cut(s) 378
PsiI TTATAA 1 cut(s) 1340
Psp124BI GAGCTC 4 cut(s) 318, 873, 1243, 1314
Psp6I CCWGG 4 cut(s) 1377, 1464, 1509, 2236
PspGI CCWGG 4 cut(s) 1377, 1464, 1509, 2236
PspN4I GGNNCC 3 cut(s) 904, 1868, 2235
PspPI GGNCC 5 cut(s) 752, 902, 1003, 2234, 2251
PstI CTGCAG 1 cut(s) 570
PstNI CAGNNNCTG 1 cut(s) 1863
PsuI RGATCY 4 cut(s) 263, 503, 535, 2149
PvuI CGATCG 1 cut(s) 714
PvuII CAGCTG 2 cut(s) 220, 1776
RsaI GTAC 3 cut(s) 458, 1798, 1988
RsaNI GTAC 3 cut(s) 457, 1797, 1987
SacI GAGCTC 4 cut(s) 318, 873, 1243, 1314
SapI GCTCTTC 3 cut(s) 207, 1231, 1763
SaqAI TTAA 7 cut(s) 378, 605, 621, 1127, 1458, 1749, 2094
SatI GCNGC 8 cut(s) 218, 221, 447, 489, 968, 1774, 1833, 2211
Sau3AI GATC 7 cut(s) 263, 503, 535, 679, 711, 1360, 2149
Sau96I GGNCC 5 cut(s) 752, 902, 1003, 2234, 2251
SchI GAGTC 1 cut(s) 1806
ScrFI CCNGG 7 cut(s) 1379, 1466, 1511, 1969, 1970, 2196, 2238
SduI GDGCHC 5 cut(s) 318, 414, 873, 1243, 1314
SexAI ACCWGGT 1 cut(s) 1464
SfaNI GCATC 5 cut(s) 61, 940, 1854, 1889, 2134
SfcI CTRYAG 3 cut(s) 566, 915, 1777
SfuI TTCGAA 2 cut(s) 1288, 1480
SinI GGWCC 4 cut(s) 752, 902, 2234, 2251
SmaI CCCGGG 1 cut(s) 1970
SmlI CTYRAG 4 cut(s) 353, 1242, 1997, 2093
SmoI CTYRAG 4 cut(s) 353, 1242, 1997, 2093
Sse9I AATT 8 cut(s) 375, 790, 960, 1031, 1129, 1166, 1574, 2184
SsiI CCGC 4 cut(s) 482, 967, 1579, 2147
SspI AATATT 1 cut(s) 672
SspMI CTAG 4 cut(s) 161, 761, 810, 2259
SstI GAGCTC 4 cut(s) 318, 873, 1243, 1314
StyD4I CCNGG 7 cut(s) 1377, 1464, 1509, 1967, 1968, 2194, 2236
StyI CCWWGG 6 cut(s) 198, 844, 977, 1215, 1890, 2023
TaaI ACNGT 9 cut(s) 119, 139, 556, 603, 777, 898, 1566, 1991, 2291
TaiI ACGT 2 cut(s) 1403, 2047
TaqI TCGA 4 cut(s) 1288, 1480, 1710, 1958
TasI AATT 8 cut(s) 375, 790, 960, 1031, 1129, 1166, 1574, 2184
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 970
TfiI GAWTC 7 cut(s) 83, 176, 703, 1172, 1707, 1818, 1908
Tru1I TTAA 7 cut(s) 378, 605, 621, 1127, 1458, 1749, 2094
Tru9I TTAA 7 cut(s) 378, 605, 621, 1127, 1458, 1749, 2094
TscAI CASTG 7 cut(s) 598, 780, 1571, 1687, 1723, 1765, 2232
TseFI GTSAC 1 cut(s) 2174
TseI GCWGC 7 cut(s) 217, 220, 446, 488, 1773, 1832, 2210
Tsp45I GTSAC 1 cut(s) 2174
TspGWI ACGGA 2 cut(s) 77, 1339
TspMI CCCGGG 1 cut(s) 1968
TspRI CASTG 7 cut(s) 598, 780, 1571, 1687, 1723, 1765, 2232
Vha464I CTTAAG 1 cut(s) 2093
VpaK11BI GGWCC 4 cut(s) 752, 902, 2234, 2251
VspI ATTAAT 1 cut(s) 378
XagI CCTNNNNNAGG 1 cut(s) 1508
XapI RAATTY 4 cut(s) 960, 1129, 1166, 1574
XceI RCATGY 3 cut(s) 889, 1845, 2012
XmaI CCCGGG 1 cut(s) 1968
XmiI GTMKAC 2 cut(s) 779, 1267
XmnI GAANNNNTTC 2 cut(s) 1373, 2220
XspI CTAG 4 cut(s) 161, 761, 810, 2259
Zsp2I ATGCAT 1 cut(s) 1847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.