Rorug07G0333200

Sulfite exporter TauE/SafE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003343
Physical Location & Seq
Reverse (-)
36534555 .. 36539132
4578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0333200.1

Sequence Viewer

Length: 1263 bp
ATGGCAAGAGTAACATGTGATCGTCTCCTTGTTAATCCATGGCGTTTATTCTCTTATCATCCCCACCAAAGCTTTGCGGTTCCCTTGCTCACTCTCACCCAGACGAAGAAGCAGCAGAAGCTCACACTCTTCTCATCTTCCAAAACGAGGACCAGAGCTTTGCCATTCGACCTCTCCCCTCCTCCGATTGACCACGACCTCTTGGACACTGTGGCAGCAACTGCAGGAGCTAAAGTCTCAGATGATGGGATTATCGAAACCTTTGATAACGACGACCAAGCTTTAGATGCTGTCGATAATGGTGTTGCGGTTGTGGACCTTTCACATTTTTGCCGGATAAGAGTTAGCGGAGAAGACCGCATCCAGTTTCTTCACAACCAAAGCACGGCCAATTTTGAATGCCTTGATGAAGGACAGGGATGTGACACCGTTTTTGTTACACCAACAGCTCGGACAATAGATATCGCACATGCATGGGTCATGAAAAATGCAGTAATGTTAATGGTTTCACCTGTTTCCAGAAGTATCTCTGAAATGCTGAAAAAGTACATATTTTTTAATGACAAGGTAGAGATTCAAGACATCACTAAGCAAACTTGCTTCTTCGTTTTAGTGGGACCTAAAAGCAATCAACTAATGGAGGAGTTACACCTAGGTGATCTCGTTGGACAACCATATGGCACCCATCAGCATTTCAGTGTCAATGGGATGCCTGTAACTGTGGGAGTGGGCAATGTGATATCTGAAAAAGGCTTTTCACTTTTGATGTCACCAGCTGCCGCTGGATCAGTCTGGAAAACTATTCTATCAGAAGGTGCAACTCCAATGGGCTCTAATGCATGGGAAAAACTAAGAATTCTCCAAGGAAAGCCAGCTCCTCAGAAGGAGCTTACTAATGAATATAATGTCCTGGAGGCTGGTCTCTGGAATTCGATCTCCTTAAGCAAAGGGTGCTATAAGGGACAAGAGACTATATCAAGACTGATAACGTATGATGGAGTCAAGCAAAGACTTTGGGGAATCTCTCTATCAGCACCAGCAGAACCTGGCAGCATCATTACAGTTGGTGGGAAAAAGGTTGGAAAGCTAACAAGTTGCACATCTGGAAGAAAGGAAAGTGAGTATTTTGGTTTAGGCTATATCAAGAGGCAAAATGTTTCAGAAGGAGACACGGTAATGGTTGGAGATGCTATTACTGGTACAGTGGTAGAAGTACCTTTTCTTGCTGGGCAACGGCCACCATCGAGAAGCTCAAGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TauE

Protein Analysis

420

Amino Acids

45.84

Weight (kDa)

6.52

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_T PF01571 92 - 316 3e-27 GCVT N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AciI CCGC 5 cut(s) 77, 308, 348, 358, 780
AclWI GGATC 1 cut(s) 793
AcoI YGGCCR 2 cut(s) 387, 1235
AcsI RAATTY 2 cut(s) 855, 928
AfaI GTAC 3 cut(s) 548, 1201, 1215
AfiI CCNNNNNNNGG 2 cut(s) 147, 385
AflII CTTAAG 1 cut(s) 940
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 2 cut(s) 398, 578
AjnI CCWGG 2 cut(s) 909, 1045
AleI CACNNNNGTG 1 cut(s) 654
Alw26I GTCTC 5 cut(s) 29, 241, 926, 962, 1161
AlwI GGATC 1 cut(s) 793
AlwNI CAGNNNCTG 2 cut(s) 221, 1046
AoxI GGCC 2 cut(s) 387, 1235
ApeKI GCWGC 4 cut(s) 112, 215, 776, 1050
ApoI RAATTY 2 cut(s) 855, 928
ArsI GACNNNNNNTTYG 4 cut(s) 142, 174, 416, 448
AspA2I CCTAGG 1 cut(s) 652
AspS9I GGNCC 3 cut(s) 150, 316, 617
AsuHPI GGTGA 4 cut(s) 88, 501, 668, 762
AvaII GGWCC 3 cut(s) 150, 316, 617
AvrII CCTAGG 1 cut(s) 652
BaeI ACNNNNGTAYC 2 cut(s) 1191, 1224
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 833
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 4 cut(s) 124, 227, 763, 1062
BccI CCATC 4 cut(s) 239, 693, 989, 1249
BceAI ACGGC 2 cut(s) 402, 1250
BciT130I CCWGG 2 cut(s) 911, 1047
BcoDI GTCTC 5 cut(s) 29, 241, 926, 962, 1161
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 940
BisI GCNGC 5 cut(s) 113, 216, 777, 780, 1051
BlnI CCTAGG 1 cut(s) 652
BlsI GCNGC 5 cut(s) 114, 217, 778, 781, 1052
Bme1390I CCNGG 2 cut(s) 911, 1047
Bme18I GGWCC 3 cut(s) 150, 316, 617
BmgT120I GGNCC 3 cut(s) 150, 316, 617
BmiI GGNNCC 3 cut(s) 81, 618, 682
BmrFI CCNGG 2 cut(s) 911, 1047
BmsI GCATC 5 cut(s) 277, 369, 699, 1062, 1177
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 932
BpuEI CTTGAG 1 cut(s) 1237
BsaI GGTCTC 1 cut(s) 926
BsaJI CCNNGG 3 cut(s) 38, 652, 862
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 1158, 1188
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 385
Bse1I ACTGG 2 cut(s) 364, 1201
Bse3DI GCAATG 1 cut(s) 739
BseBI CCWGG 2 cut(s) 911, 1047
BseDI CCNNGG 3 cut(s) 38, 652, 862
BseGI GGATG 4 cut(s) 58, 360, 425, 714
BseLI CCNNNNNNNGG 2 cut(s) 147, 385
BseMI GCAATG 1 cut(s) 739
BseMII CTCAG 2 cut(s) 252, 893
BseNI ACTGG 2 cut(s) 364, 1201
BseRI GAGGAG 3 cut(s) 171, 656, 867
BseXI GCAGC 4 cut(s) 124, 227, 763, 1062
BseYI CCCAGC 1 cut(s) 1226
BshFI GGCC 2 cut(s) 389, 1237
BshNI GGYRCC 1 cut(s) 680
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 2 cut(s) 630, 975
BslI CCNNNNNNNGG 2 cut(s) 147, 385
BsmAI GTCTC 5 cut(s) 29, 241, 926, 962, 1161
BsmBI CGTCTC 1 cut(s) 29
BsmFI GGGAC 2 cut(s) 630, 975
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 2 cut(s) 389, 1237
Bso31I GGTCTC 1 cut(s) 926
Bsp1286I GDGCHC 1 cut(s) 833
Bsp143I GATC 4 cut(s) 19, 658, 785, 933
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 5 cut(s) 77, 308, 348, 358, 780
BspANI GGCC 2 cut(s) 389, 1237
BspCNI CTCAG 2 cut(s) 251, 892
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 81, 618, 682
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 1 cut(s) 793
BspT107I GGYRCC 1 cut(s) 680
BspTI CTTAAG 1 cut(s) 940
BspTNI GGTCTC 1 cut(s) 926
BsrDI GCAATG 1 cut(s) 739
BsrI ACTGG 2 cut(s) 364, 1201
BssECI CCNNGG 3 cut(s) 38, 652, 862
BssMI GATC 4 cut(s) 19, 658, 785, 933
BssT1I CCWWGG 3 cut(s) 38, 652, 862
Bst2UI CCWGG 2 cut(s) 911, 1047
Bst4CI ACNGT 6 cut(s) 211, 430, 721, 1063, 1174, 1204
Bst6I CTCTTC 1 cut(s) 134
BstAFI CTTAAG 1 cut(s) 940
BstAPI GCANNNNNTGC 2 cut(s) 221, 951
BstC8I GCNNGC 1 cut(s) 873
BstDEI CTNAG 4 cut(s) 238, 588, 851, 879
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 58, 360, 425, 714
BstKTI GATC 4 cut(s) 22, 661, 788, 936
BstMAI GTCTC 5 cut(s) 29, 241, 926, 962, 1161
BstMBI GATC 4 cut(s) 19, 658, 785, 933
BstMWI GCNNNNNNNGC 4 cut(s) 118, 221, 287, 951
BstNI CCWGG 2 cut(s) 911, 1047
BstNSI RCATGY 2 cut(s) 18, 473
BstSCI CCNGG 2 cut(s) 909, 1045
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 124, 227, 763, 1062
BstV2I GAAGAC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 389, 1237
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 58, 360, 425, 714
BtsIMutI CAGTG 3 cut(s) 207, 703, 1209
Cac8I GCNNGC 1 cut(s) 873
CaiI CAGNNNCTG 2 cut(s) 221, 1046
CciI TCATGA 1 cut(s) 480
Cfr13I GGNCC 3 cut(s) 150, 316, 617
Csp6I GTAC 3 cut(s) 547, 1200, 1214
CviAII CATG 6 cut(s) 15, 39, 470, 474, 481, 840
CviQI GTAC 3 cut(s) 547, 1200, 1214
DdeI CTNAG 4 cut(s) 238, 588, 851, 879
DpnI GATC 4 cut(s) 21, 660, 787, 935
DpnII GATC 4 cut(s) 19, 658, 785, 933
EaeI YGGCCR 2 cut(s) 387, 1235
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 3 cut(s) 38, 652, 862
Eco24I GRGCYC 1 cut(s) 833
Eco31I GGTCTC 1 cut(s) 926
Eco32I GATATC 2 cut(s) 463, 741
Eco47I GGWCC 3 cut(s) 150, 316, 617
EcoO109I RGGNCCY 1 cut(s) 617
EcoRI GAATTC 2 cut(s) 855, 928
EcoRII CCWGG 2 cut(s) 909, 1045
EcoRV GATATC 2 cut(s) 463, 741
EcoT14I CCWWGG 3 cut(s) 38, 652, 862
EcoT22I ATGCAT 2 cut(s) 475, 841
EcoT38I GRGCYC 1 cut(s) 833
ErhI CCWWGG 3 cut(s) 38, 652, 862
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 6 cut(s) 18, 42, 473, 477, 484, 843
FaqI GGGAC 2 cut(s) 630, 975
FatI CATG 6 cut(s) 14, 38, 469, 473, 480, 839
FauNDI CATATG 1 cut(s) 676
Fnu4HI GCNGC 5 cut(s) 113, 216, 777, 780, 1051
FokI GGATG 4 cut(s) 45, 347, 432, 721
FriOI GRGCYC 1 cut(s) 833
Fsp4HI GCNGC 5 cut(s) 113, 216, 777, 780, 1051
FspBI CTAG 1 cut(s) 653
GluI GCNGC 5 cut(s) 113, 216, 777, 780, 1051
GsaI CCCAGC 1 cut(s) 1230
GsuI CTGGAG 1 cut(s) 932
HaeIII GGCC 2 cut(s) 389, 1237
HapII CCGG 1 cut(s) 334
Hin1II CATG 6 cut(s) 18, 42, 473, 477, 484, 843
HindIII AAGCTT 2 cut(s) 70, 279
HinfI GANTC 3 cut(s) 574, 999, 1020
HpaII CCGG 1 cut(s) 334
HphI GGTGA 4 cut(s) 88, 501, 668, 762
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 8 cut(s) 186, 241, 453, 532, 745, 811, 882, 1162
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 4 cut(s) 404, 806, 877, 1157
HpyCH4III ACNGT 6 cut(s) 211, 430, 721, 1063, 1174, 1204
HpyCH4IV ACGT 1 cut(s) 989
HpyCH4V TGCA 6 cut(s) 224, 473, 491, 818, 839, 1098
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 221, 287, 951
HpyF3I CTNAG 4 cut(s) 238, 588, 851, 879
HpySE526I ACGT 1 cut(s) 989
Hsp92II CATG 6 cut(s) 18, 42, 473, 477, 484, 843
Kzo9I GATC 4 cut(s) 19, 658, 785, 933
LmnI GCTCC 4 cut(s) 227, 880, 886, 1262
Lsp1109I GCAGC 4 cut(s) 124, 227, 763, 1062
LweI GCATC 5 cut(s) 277, 369, 699, 1062, 1177
MaeI CTAG 1 cut(s) 653
MaeII ACGT 1 cut(s) 989
MaeIII GTNAC 6 cut(s) 10, 422, 436, 645, 715, 768
MalI GATC 4 cut(s) 21, 660, 787, 935
MboI GATC 4 cut(s) 19, 658, 785, 933
MboII GAAGA 7 cut(s) 118, 121, 129, 362, 365, 595, 1119
MhlI GDGCHC 1 cut(s) 833
MluCI AATT 3 cut(s) 391, 855, 928
MlyI GAGTC 1 cut(s) 1008
MmeI TCCRAC 3 cut(s) 646, 1060, 1162
MnlI CCTC 9 cut(s) 141, 182, 189, 192, 209, 634, 888, 907, 1140
Mph1103I ATGCAT 2 cut(s) 475, 841
MseI TTAA 4 cut(s) 33, 500, 558, 941
MslI CAYNNNNRTG 4 cut(s) 472, 654, 696, 1175
MspA1I CMGCKG 2 cut(s) 776, 782
MspCI CTTAAG 1 cut(s) 940
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 2 cut(s) 911, 1047
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 2 cut(s) 911, 1047
MwoI GCNNNNNNNGC 4 cut(s) 118, 221, 287, 951
NcoI CCATGG 1 cut(s) 38
NdeI CATATG 1 cut(s) 676
NdeII GATC 4 cut(s) 19, 658, 785, 933
NlaIII CATG 6 cut(s) 18, 42, 473, 477, 484, 843
NlaIV GGNNCC 3 cut(s) 81, 618, 682
NmuCI GTSAC 2 cut(s) 422, 768
NsiI ATGCAT 2 cut(s) 475, 841
NspI RCATGY 2 cut(s) 18, 473
OliI CACNNNNGTG 1 cut(s) 654
PagI TCATGA 1 cut(s) 480
PciI ACATGT 1 cut(s) 14
PctI GAATGC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 574, 1020
PfoI TCCNGGA 1 cut(s) 909
PkrI GCNGC 5 cut(s) 114, 217, 778, 781, 1052
PleI GAGTC 1 cut(s) 1007
PpsI GAGTC 1 cut(s) 1007
PpuMI RGGWCCY 1 cut(s) 617
PscI ACATGT 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 909, 1045
PspFI CCCAGC 1 cut(s) 1226
PspGI CCWGG 2 cut(s) 909, 1045
PspN4I GGNNCC 3 cut(s) 81, 618, 682
PspPI GGNCC 3 cut(s) 150, 316, 617
PspPPI RGGWCCY 1 cut(s) 617
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 2 cut(s) 221, 1046
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 3 cut(s) 548, 1201, 1215
RsaNI GTAC 3 cut(s) 547, 1200, 1214
RseI CAYNNNNRTG 4 cut(s) 472, 654, 696, 1175
SaqAI TTAA 4 cut(s) 33, 500, 558, 941
SatI GCNGC 5 cut(s) 113, 216, 777, 780, 1051
Sau3AI GATC 4 cut(s) 19, 658, 785, 933
Sau96I GGNCC 3 cut(s) 150, 316, 617
SchI GAGTC 1 cut(s) 1008
ScrFI CCNGG 2 cut(s) 911, 1047
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 5 cut(s) 277, 369, 699, 1062, 1177
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 3 cut(s) 150, 316, 617
SmiMI CAYNNNNRTG 4 cut(s) 472, 654, 696, 1175
SmlI CTYRAG 2 cut(s) 940, 1252
SmoI CTYRAG 2 cut(s) 940, 1252
Sse9I AATT 3 cut(s) 391, 855, 928
SsiI CCGC 5 cut(s) 77, 308, 348, 358, 780
SspMI CTAG 1 cut(s) 653
StyD4I CCNGG 2 cut(s) 909, 1045
StyI CCWWGG 3 cut(s) 38, 652, 862
TaaI ACNGT 6 cut(s) 211, 430, 721, 1063, 1174, 1204
TaiI ACGT 1 cut(s) 992
TaqI TCGA 5 cut(s) 168, 255, 294, 932, 1244
TasI AATT 3 cut(s) 391, 855, 928
TatI WGTACW 1 cut(s) 546
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 2 cut(s) 574, 1020
Tru1I TTAA 4 cut(s) 33, 500, 558, 941
Tru9I TTAA 4 cut(s) 33, 500, 558, 941
TscAI CASTG 3 cut(s) 214, 703, 1209
TseFI GTSAC 2 cut(s) 422, 768
TseI GCWGC 4 cut(s) 112, 215, 776, 1050
Tsp45I GTSAC 2 cut(s) 422, 768
TspDTI ATGAA 3 cut(s) 423, 497, 912
TspRI CASTG 3 cut(s) 214, 703, 1209
Vha464I CTTAAG 1 cut(s) 940
VpaK11BI GGWCC 3 cut(s) 150, 316, 617
XapI RAATTY 2 cut(s) 855, 928
XceI RCATGY 2 cut(s) 18, 473
XmaJI CCTAGG 1 cut(s) 652
XspI CTAG 1 cut(s) 653
Zsp2I ATGCAT 2 cut(s) 475, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.