FvH4_1g13430

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003343
Physical Location & Seq
Reverse (-)
7378154 .. 7383551
5398 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13430.t3

Sequence Viewer

Length: 2178 bp
ATGGCGACGAAGACCGAGCCGGCGGCGGATTCTCCCACTTCGGTTCTCGAGGAAGAGGATTTGTGTGGGGAGATTGATGTGAAATTGGTGAAGGCGGAGGAGGAGCTGCTCGAGGTTCGGGTTAAGGAAGAGGAGACAGAAAGGGAGAAGGAGACACCCGTCTTGAGTGAGACTCAGTTCAGCAAGTTGGATGAGCTTCTCACTAAGACTCAGCTTTTTACAGACTTTTTGCTCGAGAAAATGGATGACATCTCTTTTGATGTTCCCGAGCAACTGAATGAACCTGAACCTGTGCAGAAAAAGAGAGGCCGCGGTACGAAAAGAAAGGCTCCTACTTACAATAATACTAAGGCCAAGAGGGCAGTTGCGGCTATGCTTACAAGATCTAAAGAGGGTGAGAAAATTGAAGATGTGAACCTAACTGAGGAGGAAAGACTTGAGAAACAGCAAAAGGAACTTGTACCTCTACTGACTGGTGGCAAATTGAAGTCTTATCAACTCAAAGGTGTAAAGTGGTTGATCTCTTTATGGCAAAATGGGCTCAATGGGATCCTTGCAGACCAAATGGGACTTGGCAAGACTATCCAGACCATAGGTTTTCTTTCTCATCTAAAATCTATGGGATTGGATGGGCCCTACTTGGTGATTGCTCCTCTTTCTACTCTTTCCAACTGGATTAATGAAATCTCAAGGTTTACGCCTTCAATTAAGGCTATAATCTATCATGGTAACAAGAAAGAAAGGGATGAGATAATAAGGAAGCACATGCCCAAATCAGTTGGCCCGAACTTCCCAATAATTGTTACTTCATATGAAGTGGCGTTGGCTGATGCAAGAAGATGTTTAAGACACTACAACTGGAAATATCTCGTGGTTGATGAAGGACACAGATTGAAAAACTCCAAGTGCAAACTAGTGCAGCAGTTGAAGTACATACCTGTAGAGAATAAGATTCTGTTGACTGGAACACCTCTCCAGAATAATTTGGCTGAGCTTTGGTCGTTGTTGAACTTTATTTTGCCGGATATATTCTCATCCCATGAAGAATTTGAGTCGTGGTTTGACCTAGAAGGAAAGTGCCATAATGAAGCAATGAAGGAAGAATTAGAAGAGAAGAGAAGAGCTCAAGTGCTACCGAAACTCCATGCAATATTGCGTCCCTTTCTTCTCCGAAGAATGAAGATAGATGTTGAGCTGATGCTTCCAAGAAAGAAGGAAATCATACTATATGCAACCATGACAGAGCATCAAAAGAAGTTTCAGGAACATCTGATCAATAAGACACTGGAGAAACATCTAATACTTGAAAAGGGAAGCCATGTAAATGGCCTGAAAGGGAAGCTGAACAATTTGATGATCCAACTTCGGAAGAACTGCAATCATCCTGACCTTCTAGAGTCGGCATTTGATGGATCATATTTCTACCCGCCTGTTGACCAGATAGTTGAGCAATGTGGGAAATTTAGCTTGCTTGAAAGACTGTTGAAGCTGCTGCTTGCCGGCAAACATAAGGTTCTGATATTCTCGCAGTGGACCAAGATTTTGGATATAATGGATTACTATTTTAGCGAAAAAGGATATGAAGTTTGTAGAATTGATGGCCATGTGAAACTGGATGATCGGAGAAGACAGATTGCTTCGTTCAATGATTTAGACAGCACTTGTAGAATATTCCTACTGAGCACAAGAGCCGGTGGACTAGGTATCAACCTTACTGCAGCTGATACCTGTATACTGTATGACAGTGATTGGAACCCTCAAATGGATTTGCAAGCCATGGATAGATGTCACAGGATTGGGCAAACCAAGCCTGTTCATGTTTACCGGTTGGCAACAGCACAATCTGTAGAGGGTCGGATGTTAAAAAGAGCTTTTAGCAAGTTGAAGCTTGAACATGTAGTTATTGGAAAAGGGAAGTTCCATCAGGAAAGAGCCAAGCCTGAGGCAGATTTCTTGGAGGAAGAGGATCTCATAGCACTTCTCCGAGATGAAGAATCTGCTGAAGACAAGATGATACAGACAGATATCACTGATGAAGAGCTGGAGAAAGTCTTGGATCGCAGTGATCTCATTGGGACTCCTCCTGATGCTGCTGATGCGCTTCCCCTGAAGGGACCTGGCTGGGAAGTGGTGGTTCCTACCGCTAGTGGGGGCATGCTCTCCTCCCTTAATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

726

Amino Acids

82.93

Weight (kDa)

6.24

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 161 - 324 5.5e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 165 - 464 5.9e-82 SNF2-related domain
DEAD PF00270 179 - 327 4.7e-06 DEAD/DEAH box helicase
HDA2-3 PF11496 410 - 628 1.5e-06 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 488 - 600 2.3e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1732
AccII CGCG 1 cut(s) 312
AciI CCGC 8 cut(s) 23, 26, 95, 310, 312, 368, 1427, 2142
AclWI GGATC 6 cut(s) 544, 557, 1351, 1420, 1974, 2064
AcoI YGGCCR 1 cut(s) 1600
AcsI RAATTY 2 cut(s) 1046, 1460
AcuI CTGAAG 2 cut(s) 2022, 2129
AfaI GTAC 3 cut(s) 316, 462, 932
AfiI CCNNNNNNNGG 4 cut(s) 424, 1762, 2111, 2148
AflIII ACRYGT 1 cut(s) 1894
AgeI ACCGGT 1 cut(s) 1824
AhlI ACTAGT 1 cut(s) 913
AjnI CCWGG 1 cut(s) 2116
Alw21I GWGCWC 2 cut(s) 1126, 1685
Alw26I GTCTC 3 cut(s) 128, 146, 164
AlwI GGATC 6 cut(s) 544, 557, 1351, 1420, 1974, 2064
Ama87I CYCGRG 4 cut(s) 47, 110, 233, 266
AoxI GGCC 6 cut(s) 307, 351, 632, 781, 1327, 1600
ApaI GGGCCC 1 cut(s) 636
ApeKI GCWGC 6 cut(s) 106, 919, 1489, 1492, 1718, 2090
ApoI RAATTY 2 cut(s) 1046, 1460
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 1525, 1557
AseI ATTAAT 1 cut(s) 678
AsiGI ACCGGT 1 cut(s) 1824
AspLEI GCGC 1 cut(s) 2101
AspS9I GGNCC 5 cut(s) 632, 633, 782, 1533, 2114
AsuHPI GGTGA 3 cut(s) 100, 407, 655
AvaI CYCGRG 4 cut(s) 47, 110, 233, 266
AvaII GGWCC 2 cut(s) 1533, 2114
AxyI CCTNAGG 1 cut(s) 1941
BaeGI GKGCMC 1 cut(s) 636
BalI TGGCCA 1 cut(s) 1602
BamHI GGATCC 1 cut(s) 549
BanII GRGCYC 3 cut(s) 543, 636, 1126
BauI CACGAG 1 cut(s) 869
BbsI GAAGAC 3 cut(s) 17, 1633, 2010
Bbv12I GWGCWC 2 cut(s) 1126, 1685
BbvI GCAGC 6 cut(s) 93, 931, 1476, 1479, 1730, 2077
BccI CCATC 4 cut(s) 623, 1403, 1592, 1929
BciT130I CCWGG 1 cut(s) 2118
BclI TGATCA 1 cut(s) 1272
BcoDI GTCTC 3 cut(s) 128, 146, 164
BcuI ACTAGT 1 cut(s) 913
BfaI CTAG 5 cut(s) 914, 1067, 1394, 1700, 2145
BfmI CTRYAG 3 cut(s) 939, 1716, 1845
BglI GCCNNNNNGGC 1 cut(s) 359
BglII AGATCT 1 cut(s) 383
BisI GCNGC 9 cut(s) 24, 107, 310, 369, 920, 1490, 1493, 1719, 2091
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 9 cut(s) 25, 108, 311, 370, 921, 1491, 1494, 1720, 2092
Bme1390I CCNGG 1 cut(s) 2118
Bme18I GGWCC 2 cut(s) 1533, 2114
BmeT110I CYCGRG 4 cut(s) 47, 110, 233, 266
BmgT120I GGNCC 5 cut(s) 632, 633, 782, 1533, 2114
BmiI GGNNCC 6 cut(s) 330, 551, 634, 1754, 2115, 2136
BmrFI CCNGG 1 cut(s) 2118
BmsI GCATC 5 cut(s) 820, 1188, 1255, 2077, 2086
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 3 cut(s) 17, 1633, 2010
BplI GAGNNNNNCTC 4 cut(s) 157, 189, 1108, 1140
BpmI CTGGAG 3 cut(s) 959, 1307, 2063
Bpu1102I GCTNAGC 1 cut(s) 990
BpuEI CTTGAG 4 cut(s) 184, 458, 673, 1110
BsaJI CCNNGG 2 cut(s) 310, 1776
BsaWI WCCGGW 1 cut(s) 1824
BsaXI ACNNNNNCTCC 2 cut(s) 1125, 1155
Bsc4I CCNNNNNNNGG 4 cut(s) 424, 1762, 2111, 2148
Bse118I RCCGGY 4 cut(s) 19, 1499, 1691, 1824
Bse1I ACTGG 6 cut(s) 478, 677, 863, 967, 1290, 1617
Bse21I CCTNAGG 1 cut(s) 1941
Bse3DI GCAATG 2 cut(s) 1098, 1457
BseBI CCWGG 1 cut(s) 2118
BseDI CCNNGG 2 cut(s) 310, 1776
BseGI GGATG 8 cut(s) 196, 250, 634, 751, 1034, 1381, 1621, 1863
BseLI CCNNNNNNNGG 4 cut(s) 424, 1762, 2111, 2148
BseMI GCAATG 2 cut(s) 1098, 1457
BseMII CTCAG 6 cut(s) 188, 224, 414, 981, 1670, 1932
BseNI ACTGG 6 cut(s) 478, 677, 863, 967, 1290, 1617
BseRI GAGGAG 7 cut(s) 113, 116, 146, 440, 642, 2070, 2152
BseSI GKGCMC 1 cut(s) 636
BseXI GCAGC 6 cut(s) 93, 931, 1476, 1479, 1730, 2077
BseYI CCCAGC 1 cut(s) 2121
BsgI GTGCAG 2 cut(s) 314, 938
Bsh1236I CGCG 1 cut(s) 312
BshFI GGCC 6 cut(s) 309, 353, 634, 783, 1329, 1602
BshTI ACCGGT 1 cut(s) 1824
BsiHKAI GWGCWC 2 cut(s) 1126, 1685
BsiHKCI CYCGRG 4 cut(s) 47, 110, 233, 266
BsiSI CCGG 5 cut(s) 20, 1022, 1500, 1692, 1825
BslFI GGGAC 4 cut(s) 582, 1143, 2089, 2127
BslI CCNNNNNNNGG 4 cut(s) 424, 1762, 2111, 2148
BsmAI GTCTC 3 cut(s) 128, 146, 164
BsmFI GGGAC 4 cut(s) 582, 1143, 2089, 2127
BsnI GGCC 6 cut(s) 309, 353, 634, 783, 1329, 1602
BsoBI CYCGRG 4 cut(s) 47, 110, 233, 266
Bsp120I GGGCCC 1 cut(s) 632
Bsp1286I GDGCHC 4 cut(s) 543, 636, 1126, 1685
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 1776
BspACI CCGC 8 cut(s) 23, 26, 95, 310, 312, 368, 1427, 2142
BspANI GGCC 6 cut(s) 309, 353, 634, 783, 1329, 1602
BspCNI CTCAG 6 cut(s) 187, 223, 415, 982, 1671, 1933
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 6 cut(s) 330, 551, 634, 1754, 2115, 2136
BspMAI CTGCAG 1 cut(s) 1720
BspPI GGATC 6 cut(s) 544, 557, 1351, 1420, 1974, 2064
BspQI GCTCTTC 2 cut(s) 1114, 2031
BsrDI GCAATG 2 cut(s) 1098, 1457
BsrFI RCCGGY 4 cut(s) 19, 1499, 1691, 1824
BsrI ACTGG 6 cut(s) 478, 677, 863, 967, 1290, 1617
BssAI RCCGGY 4 cut(s) 19, 1499, 1691, 1824
BssECI CCNNGG 2 cut(s) 310, 1776
BssNAI GTATAC 1 cut(s) 1733
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 1 cut(s) 1776
Bst1107I GTATAC 1 cut(s) 1733
Bst2BI CACGAG 1 cut(s) 869
Bst2UI CCWGG 1 cut(s) 2118
Bst4CI ACNGT 3 cut(s) 1482, 1737, 1745
Bst6I CTCTTC 7 cut(s) 48, 123, 1104, 1109, 1114, 1956, 2031
BstC8I GCNNGC 6 cut(s) 21, 1469, 1497, 1501, 1773, 2156
BstDEI CTNAG 8 cut(s) 174, 204, 210, 348, 423, 990, 1679, 1941
BstDSI CCRYGG 2 cut(s) 310, 1776
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 8 cut(s) 196, 250, 634, 751, 1034, 1381, 1621, 1863
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 2101
BstMAI GTCTC 3 cut(s) 128, 146, 164
BstMWI GCNNNNNNNGC 5 cut(s) 359, 368, 538, 1807, 2096
BstNI CCWGG 1 cut(s) 2118
BstNSI RCATGY 3 cut(s) 769, 1898, 2158
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 2116
BstSFI CTRYAG 3 cut(s) 939, 1716, 1845
BstSLI GKGCMC 1 cut(s) 636
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 6 cut(s) 93, 931, 1476, 1479, 1730, 2077
BstV2I GAAGAC 3 cut(s) 17, 1633, 2010
BstX2I RGATCY 3 cut(s) 383, 549, 1966
BstXI CCANNNNNNTGG 2 cut(s) 1325, 1543
BstYI RGATCY 3 cut(s) 383, 549, 1966
BstZ17I GTATAC 1 cut(s) 1733
Bsu36I CCTNAGG 1 cut(s) 1941
BsuRI GGCC 6 cut(s) 309, 353, 634, 783, 1329, 1602
BtgI CCRYGG 2 cut(s) 310, 1776
BtsCI GGATG 8 cut(s) 196, 250, 634, 751, 1034, 1381, 1621, 1863
BtsI GCAGTG 2 cut(s) 1535, 2068
BtsIMutI CAGTG 5 cut(s) 1283, 1535, 1750, 2028, 2068
Cac8I GCNNGC 6 cut(s) 21, 1469, 1497, 1501, 1773, 2156
CfoI GCGC 1 cut(s) 2101
Cfr10I RCCGGY 4 cut(s) 19, 1499, 1691, 1824
Cfr13I GGNCC 5 cut(s) 632, 633, 782, 1533, 2114
Cfr42I CCGCGG 1 cut(s) 313
CseI GACGC 1 cut(s) 1145
Csp6I GTAC 3 cut(s) 315, 461, 931
CspAI ACCGGT 1 cut(s) 1824
CviQI GTAC 3 cut(s) 315, 461, 931
DdeI CTNAG 8 cut(s) 174, 204, 210, 348, 423, 990, 1679, 1941
EaeI YGGCCR 1 cut(s) 1600
Eam1104I CTCTTC 7 cut(s) 48, 123, 1104, 1109, 1114, 1956, 2031
EarI CTCTTC 7 cut(s) 48, 123, 1104, 1109, 1114, 1956, 2031
EciI GGCGGA 2 cut(s) 41, 110
Ecl136II GAGCTC 1 cut(s) 1124
Eco130I CCWWGG 1 cut(s) 1776
Eco24I GRGCYC 3 cut(s) 543, 636, 1126
Eco32I GATATC 1 cut(s) 2026
Eco47I GGWCC 2 cut(s) 1533, 2114
Eco53kI GAGCTC 1 cut(s) 1124
Eco57I CTGAAG 2 cut(s) 2022, 2129
Eco81I CCTNAGG 1 cut(s) 1941
Eco88I CYCGRG 4 cut(s) 47, 110, 233, 266
EcoICRI GAGCTC 1 cut(s) 1124
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoO109I RGGNCCY 2 cut(s) 633, 2114
EcoRII CCWGG 1 cut(s) 2116
EcoRV GATATC 1 cut(s) 2026
EcoT14I CCWWGG 1 cut(s) 1776
EcoT38I GRGCYC 3 cut(s) 543, 636, 1126
ErhI CCWWGG 1 cut(s) 1776
FaqI GGGAC 4 cut(s) 582, 1143, 2089, 2127
FauI CCCGC 1 cut(s) 1434
FauNDI CATATG 1 cut(s) 811
FbaI TGATCA 1 cut(s) 1272
FblI GTMKAC 1 cut(s) 1732
Fnu4HI GCNGC 9 cut(s) 24, 107, 310, 369, 920, 1490, 1493, 1719, 2091
FokI GGATG 8 cut(s) 203, 257, 641, 758, 1021, 1368, 1628, 1870
FriOI GRGCYC 3 cut(s) 543, 636, 1126
Fsp4HI GCNGC 9 cut(s) 24, 107, 310, 369, 920, 1490, 1493, 1719, 2091
FspBI CTAG 5 cut(s) 914, 1067, 1394, 1700, 2145
GlaI GCGC 1 cut(s) 2100
GluI GCNGC 9 cut(s) 24, 107, 310, 369, 920, 1490, 1493, 1719, 2091
GsaI CCCAGC 1 cut(s) 2125
GsuI CTGGAG 3 cut(s) 959, 1307, 2063
HaeIII GGCC 6 cut(s) 309, 353, 634, 783, 1329, 1602
HapII CCGG 5 cut(s) 20, 1022, 1500, 1692, 1825
HgaI GACGC 1 cut(s) 1145
HhaI GCGC 1 cut(s) 2101
Hin6I GCGC 1 cut(s) 2099
HinP1I GCGC 1 cut(s) 2099
HincII GTYRAC 2 cut(s) 960, 1435
HindII GTYRAC 2 cut(s) 960, 1435
HindIII AAGCTT 1 cut(s) 1886
HinfI GANTC 8 cut(s) 29, 172, 208, 952, 1052, 1397, 1994, 2077
HpaII CCGG 5 cut(s) 20, 1022, 1500, 1692, 1825
HphI GGTGA 3 cut(s) 100, 407, 655
Hpy166II GTNNAC 8 cut(s) 415, 696, 960, 1435, 1533, 1697, 1733, 1822
Hpy188I TCNGA 7 cut(s) 1172, 1272, 1368, 1518, 1623, 1857, 1985
Hpy8I GTNNAC 8 cut(s) 415, 696, 960, 1435, 1533, 1697, 1733, 1822
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 9 cut(s) 85, 142, 711, 875, 1064, 1090, 1207, 1400, 2104
HpyCH4III ACNGT 3 cut(s) 1482, 1737, 1745
HpyF10VI GCNNNNNNNGC 5 cut(s) 359, 368, 538, 1807, 2096
HpyF3I CTNAG 8 cut(s) 174, 204, 210, 348, 423, 990, 1679, 1941
HspAI GCGC 1 cut(s) 2099
KroI GCCGGC 2 cut(s) 19, 1499
KroNI GCCGGC 2 cut(s) 21, 1501
Ksp22I TGATCA 1 cut(s) 1272
KspI CCGCGG 1 cut(s) 313
LguI GCTCTTC 2 cut(s) 1114, 2031
LmnI GCTCC 3 cut(s) 103, 334, 655
Lsp1109I GCAGC 6 cut(s) 93, 931, 1476, 1479, 1730, 2077
LweI GCATC 5 cut(s) 820, 1188, 1255, 2077, 2086
MaeI CTAG 5 cut(s) 914, 1067, 1394, 1700, 2145
MaeIII GTNAC 3 cut(s) 728, 802, 1787
MflI RGATCY 3 cut(s) 383, 549, 1966
MhlI GDGCHC 4 cut(s) 543, 636, 1126, 1685
MlsI TGGCCA 1 cut(s) 1602
MluNI TGGCCA 1 cut(s) 1602
MlyI GAGTC 5 cut(s) 166, 202, 1061, 1406, 2071
MmeI TCCRAC 4 cut(s) 168, 693, 1384, 1835
Mox20I TGGCCA 1 cut(s) 1602
MroNI GCCGGC 2 cut(s) 19, 1499
MscI TGGCCA 1 cut(s) 1602
MseI TTAA 6 cut(s) 123, 678, 708, 845, 1862, 2169
MslI CAYNNNNRTG 1 cut(s) 1323
Msp20I TGGCCA 1 cut(s) 1602
MspA1I CMGCKG 2 cut(s) 312, 1721
MspI CCGG 5 cut(s) 20, 1022, 1500, 1692, 1825
MspR9I CCNGG 1 cut(s) 2118
MvaI CCWGG 1 cut(s) 2118
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 5 cut(s) 359, 368, 538, 1807, 2096
NaeI GCCGGC 2 cut(s) 21, 1501
NcoI CCATGG 1 cut(s) 1776
NdeI CATATG 1 cut(s) 811
NgoMIV GCCGGC 2 cut(s) 19, 1499
NlaIV GGNNCC 6 cut(s) 330, 551, 634, 1754, 2115, 2136
NmuCI GTSAC 1 cut(s) 1787
NspI RCATGY 3 cut(s) 769, 1898, 2158
PaeI GCATGC 1 cut(s) 2158
PaeR7I CTCGAG 3 cut(s) 47, 110, 233
PciI ACATGT 1 cut(s) 1894
PciSI GCTCTTC 2 cut(s) 1114, 2031
PdiI GCCGGC 2 cut(s) 21, 1501
PfeI GAWTC 3 cut(s) 29, 952, 1994
PinAI ACCGGT 1 cut(s) 1824
PkrI GCNGC 9 cut(s) 25, 108, 311, 370, 921, 1491, 1494, 1720, 2092
PleI GAGTC 5 cut(s) 166, 202, 1060, 1405, 2071
PpsI GAGTC 5 cut(s) 166, 202, 1060, 1405, 2071
PpuMI RGGWCCY 1 cut(s) 2114
PscI ACATGT 1 cut(s) 1894
PshAI GACNNNNGTC 1 cut(s) 158
PshBI ATTAAT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 1126
Psp5II RGGWCCY 1 cut(s) 2114
Psp6I CCWGG 1 cut(s) 2116
PspFI CCCAGC 1 cut(s) 2121
PspGI CCWGG 1 cut(s) 2116
PspN4I GGNNCC 6 cut(s) 330, 551, 634, 1754, 2115, 2136
PspOMI GGGCCC 1 cut(s) 632
PspPI GGNCC 5 cut(s) 632, 633, 782, 1533, 2114
PspPPI RGGWCCY 1 cut(s) 2114
PspXI VCTCGAGB 1 cut(s) 110
PstI CTGCAG 1 cut(s) 1720
PsuI RGATCY 3 cut(s) 383, 549, 1966
PvuII CAGCTG 1 cut(s) 1721
RsaI GTAC 3 cut(s) 316, 462, 932
RsaNI GTAC 3 cut(s) 315, 461, 931
RseI CAYNNNNRTG 1 cut(s) 1323
SacI GAGCTC 1 cut(s) 1126
SacII CCGCGG 1 cut(s) 313
SapI GCTCTTC 2 cut(s) 1114, 2031
SaqAI TTAA 6 cut(s) 123, 678, 708, 845, 1862, 2169
SatI GCNGC 9 cut(s) 24, 107, 310, 369, 920, 1490, 1493, 1719, 2091
Sau96I GGNCC 5 cut(s) 632, 633, 782, 1533, 2114
SchI GAGTC 5 cut(s) 166, 202, 1061, 1406, 2071
ScrFI CCNGG 1 cut(s) 2118
SduI GDGCHC 4 cut(s) 543, 636, 1126, 1685
SfaNI GCATC 5 cut(s) 820, 1188, 1255, 2077, 2086
SfcI CTRYAG 3 cut(s) 939, 1716, 1845
Sfr274I CTCGAG 3 cut(s) 47, 110, 233
Sfr303I CCGCGG 1 cut(s) 313
SgrBI CCGCGG 1 cut(s) 313
SinI GGWCC 2 cut(s) 1533, 2114
SlaI CTCGAG 3 cut(s) 47, 110, 233
SmiMI CAYNNNNRTG 1 cut(s) 1323
SmlI CTYRAG 7 cut(s) 47, 110, 163, 233, 437, 688, 1125
SmoI CTYRAG 7 cut(s) 47, 110, 163, 233, 437, 688, 1125
SpeI ACTAGT 1 cut(s) 913
SphI GCATGC 1 cut(s) 2158
SsiI CCGC 8 cut(s) 23, 26, 95, 310, 312, 368, 1427, 2142
SspI AATATT 2 cut(s) 1152, 1671
SspMI CTAG 5 cut(s) 914, 1067, 1394, 1700, 2145
SstI GAGCTC 1 cut(s) 1126
StyD4I CCNGG 1 cut(s) 2116
StyI CCWWGG 1 cut(s) 1776
TaaI ACNGT 3 cut(s) 1482, 1737, 1745
TaqI TCGA 3 cut(s) 48, 111, 234
TaqII GACCGA 1 cut(s) 29
TatI WGTACW 1 cut(s) 930
TauI GCSGC 3 cut(s) 26, 312, 371
TfiI GAWTC 3 cut(s) 29, 952, 1994
Tru1I TTAA 6 cut(s) 123, 678, 708, 845, 1862, 2169
Tru9I TTAA 6 cut(s) 123, 678, 708, 845, 1862, 2169
TscAI CASTG 5 cut(s) 1290, 1535, 1750, 2035, 2068
TseFI GTSAC 1 cut(s) 1787
TseI GCWGC 6 cut(s) 106, 919, 1489, 1492, 1718, 2090
Tsp45I GTSAC 1 cut(s) 1787
TspRI CASTG 5 cut(s) 1290, 1535, 1750, 2035, 2068
VpaK11BI GGWCC 2 cut(s) 1533, 2114
VspI ATTAAT 1 cut(s) 678
XagI CCTNNNNNAGG 1 cut(s) 422
XapI RAATTY 2 cut(s) 1046, 1460
XbaI TCTAGA 1 cut(s) 1393
XceI RCATGY 3 cut(s) 769, 1898, 2158
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XhoI CTCGAG 3 cut(s) 47, 110, 233
XmiI GTMKAC 1 cut(s) 1732
XspI CTAG 5 cut(s) 914, 1067, 1394, 1700, 2145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.