Rh2BG155000

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003343
Physical Location & Seq
Reverse (-)
13276975 .. 13283366
6392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG155000.1

Sequence Viewer

Length: 2190 bp
ATGGCGACGAAGGCCGAGCCGTCGGCGGAGTCTCCGACTTCGGTTCTCAAGGAAGAGGATGTATGTGGGGAGATTGATGTCAAATTAGAGGAGATTGATGTCAAATTAGAGAAGGAGGAGGAGAAGCTCCTCGAAGCTCGGGTTAAGGAAGAGGAGGCAGAAACAGAAAAGGAGCCGCCCCACTTGAATGACACTCAGTTTAGCAAGTTGGATGAGCTTCTAACAAAGACTCAGCTTTACTCGGATTTTTTGCTCGAGAAAATGGATGACATCACACTTGATGTTCCCGAGCAGCCGTGTGAACCTGTTGAGAAAAAGAGAGGCCGTGGTTTCAAAAGAAAGGCTGCTTACAATAATACAAAGGCCAAGAGGGCAGTTGCAGCTATGCTTACAAGATCTAAAGAGGGTGAGAAAATTGAAGATGTGAACCTAACCGAGGAAGAAAGAGTTGAGAAACAGCAAAAGGAACTTGTACCTCTACTGACTGGTGGAAAATTGAAGTCTTATCAACTCAAAGGTGTAAAGTGGTTGATCTCTTTATGGCAAAATGGGCTGAATGGGATCCTTGCAGACCAAATGGGACTTGGAAAGACTATCCAAACCATAGGTTTTCTTTCTCATCTGAAATCTATGGGACTGGATGGGCCCTACTTGGTGATTGCACCTCTTTCTACTCTTTCCAACTGGATTAATGAAATTTCAAGGTTTACGCCTTCAATGAAGGCTATAATCTATCATGGTAACAAGAAAGAAAGGGATGACATACGAAGGAAGCACATGCCCAAATCAGTTGGCCCTAACTTCCCAATAATTGTTACCTCATATGAAGTTGCGTTGGCTGATGCAAGAAAATGTTTAAGGCACTACAACTGGAAATATCTTGTGGTTGATGAAGGACACAGATTGAAAAACTCCAAGTGCAAATTAGTGCAGCAGTTGAAGTACATACCTGTAGAGAATAAGATTCTGTTGACTGGAACACCTCTGCAGAATAATTTGGCTGAGCTTTGGTCATTGTTGAACTTTATTTTGCCGGATATATTCTCATCCCATGAAGAATTTGAGTCATGGTTTGACCTAGGAGGAAAGTGCAATAGTGAAGCAATGAAGGAAGAATTGGAAGAGAAGAGAAGGGCTCAAGTGCTACCGAAACTCCATGCCATACTGCGTCCCTTTCTTCTCCGAAGAATGAAGATAGATGTTGAGCTGATGCTTCCTAGGAAAAAGGAAATCATATTATATGCAACCATGACAGAGCATCAAAAGAAGTTTCAGGAACATCTGATCAATAAGACACTGGAGAAACATCTAATACTTGAAAATGGAAGCCATGTAAATGGCCTGAAAGGGAAGCTTAACAATTTGATGATCCAACTTCGGAAGAACTGCAATCATCCTGACCTTTTAGAGTCGGCATTTGATGGATCATATTTCTACCCCCCTGTTGACCAGATAGTTGAGCAATGTGGGAAATTTAGCTTGCTTGAACGACTGTTGAAGCGGCTGCTTGCCTGCAAACACAAGGTTCTGATATTCTCACAGTGGACTAAGATTTTGGATATAATGGATTACTATTTTAGCGAAAAAGGATTTGAAGTTTGTAGAATCGATGGCAATGTGAAACTAGATGAGCGGAGAAGACAGATTGCTTCTTTCAATGATTTAGACAGCACTTGTAGAATATTCCTTCTGAGCACAAGAGCTGGTGGACTGGGTATCAACCTTACTGCAGCTGATACTTGTATACTGTATGACAGTGATTGGAACCCTCAAATGGATTTGCAAGCCATGGATAGATGTCACAGGATTGGGCAAACCAAGCCGGTTCATGTTTACCGGTTGGCAACAGCACAATCTGTAGAGGGTCGGATGTTGAAAAGAGCTTTTAGTAAGTTGAAGCTTGAACATGTAGTCATTGGAAAAGGGAAGTTCCATCAGGAAAGAGCCAAGCCTGAGGCAGAATTCTTGGAGGAAGAGGATCTCATAGCAGTTCTCCGAGAAGAAGAATCTGCTGAGGACAAGATGATACAGACAGAAATCAGTGACGAAGAACTGGAGAAGGTCTTGGATCGCAGTGATCTCGTGGGGACTCCTACTGATGCTGCTGCTGCACTTCCCCTAAAGGGGCCTGGCTGGGAAGTGGTGGTTCCTACCGCTGGAGGGGGCATGCTCTCCACCCTTAATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

729

Amino Acids

83.29

Weight (kDa)

6.37

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 165 - 328 1.2e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 169 - 468 1.5e-81 SNF2-related domain
DEAD PF00270 183 - 331 3.4e-06 DEAD/DEAH box helicase
Helicase_C PF00271 491 - 604 1.6e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1633
AccI GTMKAC 1 cut(s) 1744
AciI CCGC 5 cut(s) 26, 176, 1501, 1633, 2154
AclWI GGATC 6 cut(s) 556, 569, 1363, 1432, 1986, 2076
AcsI RAATTY 4 cut(s) 696, 1058, 1472, 1961
AfaI GTAC 2 cut(s) 474, 944
AfiI CCNNNNNNNGG 6 cut(s) 436, 1774, 2123, 2124, 2156, 2160
AflIII ACRYGT 1 cut(s) 1906
AgeI ACCGGT 1 cut(s) 1836
AjnI CCWGG 1 cut(s) 2128
AjuI GAANNNNNNNTTGG 2 cut(s) 1100, 1132
Alw21I GWGCWC 1 cut(s) 1697
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 6 cut(s) 556, 569, 1363, 1432, 1986, 2076
Ama87I CYCGRG 3 cut(s) 138, 254, 287
AoxI GGCC 7 cut(s) 12, 322, 363, 644, 793, 1339, 2126
ApaI GGGCCC 1 cut(s) 648
ApeKI GCWGC 9 cut(s) 292, 344, 380, 931, 1504, 1730, 2102, 2105, 2108
ApoI RAATTY 4 cut(s) 696, 1058, 1472, 1961
ArsI GACNNNNNNTTYG 2 cut(s) 1537, 1569
AseI ATTAAT 1 cut(s) 690
AsiGI ACCGGT 1 cut(s) 1836
AspA2I CCTAGG 2 cut(s) 1078, 1217
AspS9I GGNCC 4 cut(s) 644, 645, 794, 2126
AsuHPI GGTGA 2 cut(s) 419, 667
AvaI CYCGRG 3 cut(s) 138, 254, 287
AvrII CCTAGG 2 cut(s) 1078, 1217
AxyI CCTNAGG 1 cut(s) 1953
BaeGI GKGCMC 1 cut(s) 648
BamHI GGATCC 1 cut(s) 561
BanII GRGCYC 2 cut(s) 648, 1138
BauI CACGAG 1 cut(s) 2081
BbsI GAAGAC 1 cut(s) 1645
Bbv12I GWGCWC 1 cut(s) 1697
BbvCI CCTCAGC 1 cut(s) 2013
BbvI GCAGC 9 cut(s) 304, 331, 392, 943, 1491, 1742, 2089, 2092, 2095
BccI CCATC 4 cut(s) 635, 1415, 1604, 1941
BceAI ACGGC 3 cut(s) 4, 280, 309
BcgI CGANNNNNNTGC 2 cut(s) 2062, 2096
BciT130I CCWGG 1 cut(s) 2130
BclI TGATCA 1 cut(s) 1284
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 3 cut(s) 1079, 1218, 1625
BfmI CTRYAG 4 cut(s) 951, 986, 1728, 1857
BglI GCCNNNNNGGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 395
BlnI CCTAGG 2 cut(s) 1078, 1217
BlpI GCTNAGC 1 cut(s) 1002
Bme1390I CCNGG 1 cut(s) 2130
BmeT110I CYCGRG 3 cut(s) 138, 254, 287
BmgT120I GGNCC 4 cut(s) 644, 645, 794, 2126
BmiI GGNNCC 6 cut(s) 174, 563, 646, 1766, 2127, 2148
BmrFI CCNGG 1 cut(s) 2130
BmrI ACTGGG 1 cut(s) 1721
BmsI GCATC 4 cut(s) 832, 1200, 1267, 2089
BmuI ACTGGG 1 cut(s) 1721
BpiI GAAGAC 1 cut(s) 1645
BplI GAGNNNNNCTC 2 cut(s) 1120, 1152
BpmI CTGGAG 3 cut(s) 1319, 2075, 2178
Bpu10I CCTNAGC 1 cut(s) 2013
Bpu1102I GCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 2 cut(s) 32, 1122
Bsa29I ATCGAT 1 cut(s) 1608
BsaJI CCNNGG 5 cut(s) 325, 435, 1078, 1217, 1788
BsaWI WCCGGW 1 cut(s) 1836
BsaXI ACNNNNNCTCC 2 cut(s) 1137, 1167
Bsc4I CCNNNNNNNGG 6 cut(s) 436, 1774, 2123, 2124, 2156, 2160
Bse118I RCCGGY 2 cut(s) 1822, 1836
Bse1I ACTGG 8 cut(s) 490, 642, 689, 875, 979, 1302, 1716, 2058
Bse21I CCTNAGG 1 cut(s) 1953
Bse3DI GCAATG 3 cut(s) 1110, 1469, 1621
BseBI CCWGG 1 cut(s) 2130
BseCI ATCGAT 1 cut(s) 1608
BseDI CCNNGG 5 cut(s) 325, 435, 1078, 1217, 1788
BseGI GGATG 8 cut(s) 64, 217, 271, 646, 763, 1046, 1393, 1875
BseLI CCNNNNNNNGG 6 cut(s) 436, 1774, 2123, 2124, 2156, 2160
BseMI GCAATG 3 cut(s) 1110, 1469, 1621
BseMII CTCAG 6 cut(s) 209, 245, 993, 1682, 1944, 2004
BseNI ACTGG 8 cut(s) 490, 642, 689, 875, 979, 1302, 1716, 2058
BseRI GAGGAG 5 cut(s) 104, 119, 131, 134, 167
BseSI GKGCMC 1 cut(s) 648
BseXI GCAGC 9 cut(s) 304, 331, 392, 943, 1491, 1742, 2089, 2092, 2095
BseYI CCCAGC 1 cut(s) 2133
BsgI GTGCAG 2 cut(s) 950, 2094
BshFI GGCC 7 cut(s) 14, 324, 365, 646, 795, 1341, 2128
BshTI ACCGGT 1 cut(s) 1836
BshVI ATCGAT 1 cut(s) 1608
BsiHKAI GWGCWC 1 cut(s) 1697
BsiHKCI CYCGRG 3 cut(s) 138, 254, 287
BsiSI CCGG 3 cut(s) 1034, 1823, 1837
BslFI GGGAC 4 cut(s) 594, 648, 1155, 2101
BslI CCNNNNNNNGG 6 cut(s) 436, 1774, 2123, 2124, 2156, 2160
BsmAI GTCTC 1 cut(s) 36
BsmFI GGGAC 4 cut(s) 594, 648, 1155, 2101
BsnI GGCC 7 cut(s) 14, 324, 365, 646, 795, 1341, 2128
BsoBI CYCGRG 3 cut(s) 138, 254, 287
Bsp120I GGGCCC 1 cut(s) 644
Bsp1286I GDGCHC 3 cut(s) 648, 1138, 1697
Bsp143I GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
Bsp1720I GCTNAGC 1 cut(s) 1002
Bsp19I CCATGG 1 cut(s) 1788
BspACI CCGC 5 cut(s) 26, 176, 1501, 1633, 2154
BspANI GGCC 7 cut(s) 14, 324, 365, 646, 795, 1341, 2128
BspCNI CTCAG 6 cut(s) 208, 244, 994, 1683, 1945, 2005
BspDI ATCGAT 1 cut(s) 1608
BspLI GGNNCC 6 cut(s) 174, 563, 646, 1766, 2127, 2148
BspMAI CTGCAG 2 cut(s) 990, 1732
BspPI GGATC 6 cut(s) 556, 569, 1363, 1432, 1986, 2076
BsrBI CCGCTC 1 cut(s) 1633
BsrDI GCAATG 3 cut(s) 1110, 1469, 1621
BsrFI RCCGGY 2 cut(s) 1822, 1836
BsrI ACTGG 8 cut(s) 490, 642, 689, 875, 979, 1302, 1716, 2058
BssAI RCCGGY 2 cut(s) 1822, 1836
BssECI CCNNGG 5 cut(s) 325, 435, 1078, 1217, 1788
BssMI GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
BssNAI GTATAC 1 cut(s) 1745
BssSI CACGAG 1 cut(s) 2081
BssT1I CCWWGG 3 cut(s) 1078, 1217, 1788
Bst1107I GTATAC 1 cut(s) 1745
Bst2BI CACGAG 1 cut(s) 2081
Bst2UI CCWGG 1 cut(s) 2130
Bst4CI ACNGT 4 cut(s) 1494, 1542, 1749, 1757
Bst6I CTCTTC 5 cut(s) 48, 144, 1116, 1121, 1968
BstC8I GCNNGC 5 cut(s) 1481, 1509, 1513, 1785, 2168
BstDEI CTNAG 7 cut(s) 195, 231, 1002, 1548, 1691, 1953, 2013
BstDSI CCRYGG 2 cut(s) 325, 1788
BstENI CCTNNNNNAGG 1 cut(s) 434
BstF5I GGATG 8 cut(s) 64, 217, 271, 646, 763, 1046, 1393, 1875
BstKTI GATC 9 cut(s) 398, 534, 564, 1287, 1371, 1427, 1981, 2071, 2080
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
BstMWI GCNNNNNNNGC 6 cut(s) 11, 371, 380, 550, 1819, 2108
BstNI CCWGG 1 cut(s) 2130
BstNSI RCATGY 3 cut(s) 781, 1910, 2170
BstSCI CCNGG 1 cut(s) 2128
BstSFI CTRYAG 4 cut(s) 951, 986, 1728, 1857
BstSLI GKGCMC 1 cut(s) 648
BstV1I GCAGC 9 cut(s) 304, 331, 392, 943, 1491, 1742, 2089, 2092, 2095
BstV2I GAAGAC 1 cut(s) 1645
BstX2I RGATCY 3 cut(s) 395, 561, 1978
BstXI CCANNNNNNTGG 1 cut(s) 1337
BstYI RGATCY 3 cut(s) 395, 561, 1978
BstZ17I GTATAC 1 cut(s) 1745
Bsu15I ATCGAT 1 cut(s) 1608
Bsu36I CCTNAGG 1 cut(s) 1953
BsuRI GGCC 7 cut(s) 14, 324, 365, 646, 795, 1341, 2128
BsuTUI ATCGAT 1 cut(s) 1608
BtgI CCRYGG 2 cut(s) 325, 1788
BtsCI GGATG 8 cut(s) 64, 217, 271, 646, 763, 1046, 1393, 1875
BtsI GCAGTG 1 cut(s) 2080
BtsIMutI CAGTG 5 cut(s) 1295, 1547, 1762, 2047, 2080
Cac8I GCNNGC 5 cut(s) 1481, 1509, 1513, 1785, 2168
Cfr10I RCCGGY 2 cut(s) 1822, 1836
Cfr13I GGNCC 4 cut(s) 644, 645, 794, 2126
ClaI ATCGAT 1 cut(s) 1608
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 2 cut(s) 473, 943
CspAI ACCGGT 1 cut(s) 1836
CviQI GTAC 2 cut(s) 473, 943
DdeI CTNAG 7 cut(s) 195, 231, 1002, 1548, 1691, 1953, 2013
DpnI GATC 9 cut(s) 397, 533, 563, 1286, 1370, 1426, 1980, 2070, 2079
DpnII GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
Eam1104I CTCTTC 5 cut(s) 48, 144, 1116, 1121, 1968
EarI CTCTTC 5 cut(s) 48, 144, 1116, 1121, 1968
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 1078, 1217, 1788
Eco24I GRGCYC 2 cut(s) 648, 1138
Eco81I CCTNAGG 1 cut(s) 1953
Eco88I CYCGRG 3 cut(s) 138, 254, 287
EcoNI CCTNNNNNAGG 1 cut(s) 434
EcoO109I RGGNCCY 2 cut(s) 645, 2126
EcoRI GAATTC 1 cut(s) 1961
EcoRII CCWGG 1 cut(s) 2128
EcoT14I CCWWGG 3 cut(s) 1078, 1217, 1788
EcoT38I GRGCYC 2 cut(s) 648, 1138
ErhI CCWWGG 3 cut(s) 1078, 1217, 1788
FalI AAGNNNNNCTT 2 cut(s) 1338, 1370
FaqI GGGAC 4 cut(s) 594, 648, 1155, 2101
FauNDI CATATG 1 cut(s) 823
FbaI TGATCA 1 cut(s) 1284
FblI GTMKAC 1 cut(s) 1744
FokI GGATG 8 cut(s) 71, 224, 278, 653, 770, 1033, 1380, 1882
FriOI GRGCYC 2 cut(s) 648, 1138
FspBI CTAG 3 cut(s) 1079, 1218, 1625
GsaI CCCAGC 1 cut(s) 2137
GsuI CTGGAG 3 cut(s) 1319, 2075, 2178
HaeIII GGCC 7 cut(s) 14, 324, 365, 646, 795, 1341, 2128
HapII CCGG 3 cut(s) 1034, 1823, 1837
HgaI GACGC 1 cut(s) 1157
HincII GTYRAC 2 cut(s) 972, 1447
HindII GTYRAC 2 cut(s) 972, 1447
HindIII AAGCTT 2 cut(s) 1352, 1898
HinfI GANTC 8 cut(s) 29, 229, 964, 1064, 1409, 1605, 2006, 2089
HpaII CCGG 3 cut(s) 1034, 1823, 1837
HphI GGTGA 2 cut(s) 419, 667
Hpy166II GTNNAC 9 cut(s) 302, 427, 708, 972, 1447, 1545, 1709, 1745, 1834
Hpy188III TCNNGA 5 cut(s) 256, 287, 1274, 1397, 1937
Hpy8I GTNNAC 9 cut(s) 302, 427, 708, 972, 1447, 1545, 1709, 1745, 1834
Hpy99I CGWCG 2 cut(s) 10, 25
HpyCH4III ACNGT 4 cut(s) 1494, 1542, 1749, 1757
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 371, 380, 550, 1819, 2108
HpyF3I CTNAG 7 cut(s) 195, 231, 1002, 1548, 1691, 1953, 2013
Ksp22I TGATCA 1 cut(s) 1284
Kzo9I GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
LmnI GCTCC 2 cut(s) 132, 172
Lsp1109I GCAGC 9 cut(s) 304, 331, 392, 943, 1491, 1742, 2089, 2092, 2095
LweI GCATC 4 cut(s) 832, 1200, 1267, 2089
MaeI CTAG 3 cut(s) 1079, 1218, 1625
MaeIII GTNAC 4 cut(s) 740, 814, 1799, 2042
MalI GATC 9 cut(s) 397, 533, 563, 1286, 1370, 1426, 1980, 2070, 2079
MbiI CCGCTC 1 cut(s) 1633
MboI GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
MflI RGATCY 3 cut(s) 395, 561, 1978
MhlI GDGCHC 3 cut(s) 648, 1138, 1697
MlyI GAGTC 5 cut(s) 38, 223, 1073, 1418, 2083
MmeI TCCRAC 5 cut(s) 59, 189, 705, 1396, 1847
MseI TTAA 5 cut(s) 144, 690, 857, 1356, 2181
MslI CAYNNNNRTG 2 cut(s) 186, 1335
MspA1I CMGCKG 2 cut(s) 1733, 2156
MspI CCGG 3 cut(s) 1034, 1823, 1837
MspR9I CCNGG 1 cut(s) 2130
MvaI CCWGG 1 cut(s) 2130
MwoI GCNNNNNNNGC 6 cut(s) 11, 371, 380, 550, 1819, 2108
NcoI CCATGG 1 cut(s) 1788
NdeI CATATG 1 cut(s) 823
NdeII GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
NlaIV GGNNCC 6 cut(s) 174, 563, 646, 1766, 2127, 2148
NmeAIII GCCGAG 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 1799, 2042
NspI RCATGY 3 cut(s) 781, 1910, 2170
PaeI GCATGC 1 cut(s) 2170
PaeR7I CTCGAG 1 cut(s) 254
PciI ACATGT 1 cut(s) 1906
PfeI GAWTC 3 cut(s) 964, 1605, 2006
PinAI ACCGGT 1 cut(s) 1836
PleI GAGTC 5 cut(s) 37, 223, 1072, 1417, 2083
PpsI GAGTC 5 cut(s) 37, 223, 1072, 1417, 2083
PscI ACATGT 1 cut(s) 1906
PshBI ATTAAT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 2128
PspFI CCCAGC 1 cut(s) 2133
PspGI CCWGG 1 cut(s) 2128
PspN4I GGNNCC 6 cut(s) 174, 563, 646, 1766, 2127, 2148
PspOMI GGGCCC 1 cut(s) 644
PspPI GGNCC 4 cut(s) 644, 645, 794, 2126
PstI CTGCAG 2 cut(s) 990, 1732
PsuI RGATCY 3 cut(s) 395, 561, 1978
PvuII CAGCTG 1 cut(s) 1733
RsaI GTAC 2 cut(s) 474, 944
RsaNI GTAC 2 cut(s) 473, 943
RseI CAYNNNNRTG 2 cut(s) 186, 1335
SaqAI TTAA 5 cut(s) 144, 690, 857, 1356, 2181
Sau3AI GATC 9 cut(s) 395, 531, 561, 1284, 1368, 1424, 1978, 2068, 2077
Sau96I GGNCC 4 cut(s) 644, 645, 794, 2126
SchI GAGTC 5 cut(s) 38, 223, 1073, 1418, 2083
ScrFI CCNGG 1 cut(s) 2130
SduI GDGCHC 3 cut(s) 648, 1138, 1697
SfaNI GCATC 4 cut(s) 832, 1200, 1267, 2089
SfcI CTRYAG 4 cut(s) 951, 986, 1728, 1857
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 186, 1335
SmlI CTYRAG 3 cut(s) 47, 254, 1137
SmoI CTYRAG 3 cut(s) 47, 254, 1137
SphI GCATGC 1 cut(s) 2170
SsiI CCGC 5 cut(s) 26, 176, 1501, 1633, 2154
SspI AATATT 1 cut(s) 1683
SspMI CTAG 3 cut(s) 1079, 1218, 1625
StyD4I CCNGG 1 cut(s) 2128
StyI CCWWGG 3 cut(s) 1078, 1217, 1788
TaaI ACNGT 4 cut(s) 1494, 1542, 1749, 1757
TaqI TCGA 3 cut(s) 132, 255, 1608
TatI WGTACW 1 cut(s) 942
TauI GCSGC 2 cut(s) 178, 1504
TfiI GAWTC 3 cut(s) 964, 1605, 2006
Tru1I TTAA 5 cut(s) 144, 690, 857, 1356, 2181
Tru9I TTAA 5 cut(s) 144, 690, 857, 1356, 2181
TscAI CASTG 5 cut(s) 1302, 1547, 1762, 2047, 2080
TseFI GTSAC 2 cut(s) 1799, 2042
TseI GCWGC 9 cut(s) 292, 344, 380, 931, 1504, 1730, 2102, 2105, 2108
Tsp45I GTSAC 2 cut(s) 1799, 2042
TspDTI ATGAA 8 cut(s) 708, 734, 840, 906, 1068, 1121, 1205, 1817
TspRI CASTG 5 cut(s) 1302, 1547, 1762, 2047, 2080
VspI ATTAAT 1 cut(s) 690
XagI CCTNNNNNAGG 1 cut(s) 434
XapI RAATTY 4 cut(s) 696, 1058, 1472, 1961
XceI RCATGY 3 cut(s) 781, 1910, 2170
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XhoI CTCGAG 1 cut(s) 254
XmaJI CCTAGG 2 cut(s) 1078, 1217
XmiI GTMKAC 1 cut(s) 1744
XspI CTAG 3 cut(s) 1079, 1218, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.