Rroxscaffold_2G00141020

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003343
Physical Location & Seq
Forward (+)
79356185 .. 79362290
6106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141020.1

Sequence Viewer

Length: 1344 bp
ATGGCGACGAAGACCGAGCCGTCGGCGGAGTCTCCGACTTCGGTTCTCAAGGAAGAGGATGTGTGTGGGGAGATTGATGTCAAATTAGAGAAGGAGGAGGAGAAGCTCCTCGAAGCTCGGGTTACGGAAGAGGAGGCAGAAACAGAAAAGGAGCCGCCCCACTTGAATGACATTCAGTTTAGCAAGTTGGATGAGCTTCTAACAAAGACTCAGCTTTACTCGGATTTTTTGCTCGAGAAAATGGATGACATCACACTTGATGTTCCCGAGCAGCCGTGTGAACCTGTTGAGAAAAAGAGAGGCCGTGGTTTCAAAAGAAAGGCTGCTTACAATAATACAAAGGCCAAGAGGGCAGTTGCAGCTATGCTTACAAGATCTAAAGAGGGTGAGAAAATTGAAGATGGGAACCTAACCGAGGAAGAAAGAGTCGAGAAACAGCAAAAGGAACTTGTACCTCTACTGACTGGAGGAAAATTGAAGTCTTATCAACTCAAAGGTGTAAAGTGGTTGATCTCTTTATGGCAAAATGGGCTGAATGGGATCCTTGCAGACCAAATGGGACTTGGAAAGACTATCCAAACCATTGGTTTTCTTTCTCATCTTAAATCTATGGGACTGGATGGGCCCTACTTGGTGATTGCACCTCTTTCTACTCTTTCCAATTGGATTAATGAAATTTCAAGGTTTACGCCTTCAATGAAGGCTATAATCTATCATGGTAACAAGAAAGAAAGGGATGACATACGAAGGAAGCACATGCCCAAATCAGTTGGCCCTAACTTCCCAATAATTGTTACCTCATATGAAGTTGCGTTGGCTGATGCAAGAAAATGTTTAAGGCACTACAACTGGAAATATCTTGTGGTTGATGAAGGACACAGATTGAAAAACTCCAAGTGCAAATTAGTGCAGCAGTTGAAGTACATACCTGTAGAGAATAAGATTCTGTTGACTGGAACACCTCTGCAGAATAATTTGGCTGAGCTTTGGTCATTGTTGAACTTTATTTTGCCGGATATATTCTCATCCCATGAAGAATTTGAGTCATGGTTTGACCTAGGAGGAAAGTGCAATAGTGAAGCAATGAAGGAAGAATTGGAAGAGAAGAGAAGGGCTCAAGTGCTACCGAAACTCCATGCCATACTGCGTCCCTTTCTTCTCCGAAGAATGAAGATAGATGTTGAGCTGATGCTTCCTAGGAAAAAGGAAATCATATTATATGCAACCATGACAGAGCATCAAAAGAAGTTTCAGGAACATCTGATCAATAAGACACTGGAGAAACATCTAATGCTTGAAAATGGAAGCCATGTAAATGGCCTGAAAGGAAGCTTAACAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

447

Amino Acids

51.31

Weight (kDa)

8.06

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 158 - 321 4.9e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 162 - 428 2.3e-77 SNF2-related domain
DEAD PF00270 176 - 324 1.4e-06 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 19
AciI CCGC 2 cut(s) 26, 155
AclWI GGATC 2 cut(s) 535, 548
AcsI RAATTY 2 cut(s) 675, 1037
AfaI GTAC 2 cut(s) 453, 923
AfiI CCNNNNNNNGG 1 cut(s) 415
AjuI GAANNNNNNNTTGG 2 cut(s) 1079, 1111
AluBI AGCT 8 cut(s) 106, 116, 196, 214, 362, 985, 1186, 1332
AluI AGCT 8 cut(s) 106, 116, 196, 214, 362, 985, 1186, 1332
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 2 cut(s) 535, 548
Ama87I CYCGRG 3 cut(s) 117, 233, 266
AoxI GGCC 5 cut(s) 301, 342, 623, 772, 1318
ApaI GGGCCC 1 cut(s) 627
ApeKI GCWGC 4 cut(s) 271, 323, 359, 910
ApoI RAATTY 2 cut(s) 675, 1037
AseI ATTAAT 1 cut(s) 669
AspA2I CCTAGG 2 cut(s) 1057, 1196
AspS9I GGNCC 3 cut(s) 623, 624, 773
AsuHPI GGTGA 2 cut(s) 398, 646
AvaI CYCGRG 3 cut(s) 117, 233, 266
AvrII CCTAGG 2 cut(s) 1057, 1196
BaeGI GKGCMC 1 cut(s) 627
BamHI GGATCC 1 cut(s) 540
BanII GRGCYC 2 cut(s) 627, 1117
BbsI GAAGAC 1 cut(s) 17
BbvI GCAGC 4 cut(s) 283, 310, 371, 922
BccI CCATC 2 cut(s) 395, 614
BceAI ACGGC 3 cut(s) 4, 259, 288
BclI TGATCA 1 cut(s) 1263
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 2 cut(s) 1058, 1197
BfmI CTRYAG 2 cut(s) 930, 965
BglI GCCNNNNNGGC 1 cut(s) 350
BglII AGATCT 1 cut(s) 374
BisI GCNGC 5 cut(s) 155, 272, 324, 360, 911
BlnI CCTAGG 2 cut(s) 1057, 1196
BlpI GCTNAGC 1 cut(s) 981
BlsI GCNGC 5 cut(s) 156, 273, 325, 361, 912
BmeT110I CYCGRG 3 cut(s) 117, 233, 266
BmgT120I GGNCC 3 cut(s) 623, 624, 773
BmiI GGNNCC 4 cut(s) 153, 407, 542, 625
BmsI GCATC 3 cut(s) 811, 1179, 1246
BpiI GAAGAC 1 cut(s) 17
BplI GAGNNNNNCTC 2 cut(s) 1099, 1131
BpmI CTGGAG 2 cut(s) 486, 1298
Bpu1102I GCTNAGC 1 cut(s) 981
BpuEI CTTGAG 2 cut(s) 32, 1101
BsaJI CCNNGG 4 cut(s) 304, 414, 1057, 1196
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 5 cut(s) 469, 621, 854, 958, 1281
Bse3DI GCAATG 1 cut(s) 1089
BseDI CCNNGG 4 cut(s) 304, 414, 1057, 1196
BseGI GGATG 6 cut(s) 64, 196, 250, 625, 742, 1025
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 1089
BseMII CTCAG 2 cut(s) 224, 972
BseNI ACTGG 5 cut(s) 469, 621, 854, 958, 1281
BseRI GAGGAG 4 cut(s) 98, 110, 113, 146
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 4 cut(s) 283, 310, 371, 922
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 5 cut(s) 303, 344, 625, 774, 1320
BsiHKCI CYCGRG 3 cut(s) 117, 233, 266
BsiSI CCGG 1 cut(s) 1013
BslFI GGGAC 3 cut(s) 573, 627, 1134
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 36
BsmFI GGGAC 3 cut(s) 573, 627, 1134
BsnI GGCC 5 cut(s) 303, 344, 625, 774, 1320
BsoBI CYCGRG 3 cut(s) 117, 233, 266
Bsp120I GGGCCC 1 cut(s) 623
Bsp1286I GDGCHC 2 cut(s) 627, 1117
Bsp143I GATC 4 cut(s) 374, 510, 540, 1263
Bsp1720I GCTNAGC 1 cut(s) 981
BspACI CCGC 2 cut(s) 26, 155
BspANI GGCC 5 cut(s) 303, 344, 625, 774, 1320
BspCNI CTCAG 2 cut(s) 223, 973
BspLI GGNNCC 4 cut(s) 153, 407, 542, 625
BspMAI CTGCAG 1 cut(s) 969
BspPI GGATC 2 cut(s) 535, 548
BsrDI GCAATG 1 cut(s) 1089
BsrI ACTGG 5 cut(s) 469, 621, 854, 958, 1281
BssECI CCNNGG 4 cut(s) 304, 414, 1057, 1196
BssMI GATC 4 cut(s) 374, 510, 540, 1263
BssT1I CCWWGG 2 cut(s) 1057, 1196
Bst6I CTCTTC 4 cut(s) 48, 123, 1095, 1100
BstDEI CTNAG 2 cut(s) 210, 981
BstDSI CCRYGG 1 cut(s) 304
BstENI CCTNNNNNAGG 1 cut(s) 413
BstF5I GGATG 6 cut(s) 64, 196, 250, 625, 742, 1025
BstKTI GATC 4 cut(s) 377, 513, 543, 1266
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 4 cut(s) 374, 510, 540, 1263
BstMWI GCNNNNNNNGC 3 cut(s) 350, 359, 529
BstNSI RCATGY 1 cut(s) 760
BstSFI CTRYAG 2 cut(s) 930, 965
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 4 cut(s) 283, 310, 371, 922
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 374, 540
BstXI CCANNNNNNTGG 2 cut(s) 584, 1316
BstYI RGATCY 2 cut(s) 374, 540
BsuRI GGCC 5 cut(s) 303, 344, 625, 774, 1320
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 6 cut(s) 64, 196, 250, 625, 742, 1025
BtsIMutI CAGTG 1 cut(s) 1274
Cfr13I GGNCC 3 cut(s) 623, 624, 773
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 2 cut(s) 452, 922
CviAII CATG 7 cut(s) 716, 757, 1031, 1047, 1136, 1228, 1310
CviQI GTAC 2 cut(s) 452, 922
DdeI CTNAG 2 cut(s) 210, 981
DpnI GATC 4 cut(s) 376, 512, 542, 1265
DpnII GATC 4 cut(s) 374, 510, 540, 1263
DrdI GACNNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 19
Eam1104I CTCTTC 4 cut(s) 48, 123, 1095, 1100
EarI CTCTTC 4 cut(s) 48, 123, 1095, 1100
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 1057, 1196
Eco24I GRGCYC 2 cut(s) 627, 1117
Eco88I CYCGRG 3 cut(s) 117, 233, 266
EcoNI CCTNNNNNAGG 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 624
EcoT14I CCWWGG 2 cut(s) 1057, 1196
EcoT38I GRGCYC 2 cut(s) 627, 1117
ErhI CCWWGG 2 cut(s) 1057, 1196
FaeI CATG 7 cut(s) 719, 760, 1034, 1050, 1139, 1231, 1313
FaqI GGGAC 3 cut(s) 573, 627, 1134
FatI CATG 7 cut(s) 715, 756, 1030, 1046, 1135, 1227, 1309
FauNDI CATATG 1 cut(s) 802
FbaI TGATCA 1 cut(s) 1263
Fnu4HI GCNGC 5 cut(s) 155, 272, 324, 360, 911
FokI GGATG 6 cut(s) 71, 203, 257, 632, 749, 1012
FriOI GRGCYC 2 cut(s) 627, 1117
Fsp4HI GCNGC 5 cut(s) 155, 272, 324, 360, 911
FspBI CTAG 2 cut(s) 1058, 1197
GluI GCNGC 5 cut(s) 155, 272, 324, 360, 911
GsuI CTGGAG 2 cut(s) 486, 1298
HaeIII GGCC 5 cut(s) 303, 344, 625, 774, 1320
HapII CCGG 1 cut(s) 1013
HgaI GACGC 1 cut(s) 1136
Hin1II CATG 7 cut(s) 719, 760, 1034, 1050, 1139, 1231, 1313
HincII GTYRAC 1 cut(s) 951
HindII GTYRAC 1 cut(s) 951
HindIII AAGCTT 1 cut(s) 1330
HinfI GANTC 5 cut(s) 29, 208, 426, 943, 1043
HpaII CCGG 1 cut(s) 1013
HphI GGTGA 2 cut(s) 398, 646
Hpy166II GTNNAC 3 cut(s) 281, 687, 951
Hpy188I TCNGA 4 cut(s) 36, 223, 1163, 1263
Hpy188III TCNNGA 4 cut(s) 235, 266, 430, 1253
Hpy8I GTNNAC 3 cut(s) 281, 687, 951
Hpy99I CGWCG 2 cut(s) 10, 25
HpyAV CCTTC 7 cut(s) 85, 694, 702, 741, 866, 1081, 1104
HpyCH4V TGCA 9 cut(s) 359, 548, 641, 824, 900, 910, 967, 1071, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 359, 529
HpyF3I CTNAG 2 cut(s) 210, 981
Hsp92II CATG 7 cut(s) 719, 760, 1034, 1050, 1139, 1231, 1313
Ksp22I TGATCA 1 cut(s) 1263
Kzo9I GATC 4 cut(s) 374, 510, 540, 1263
LmnI GCTCC 2 cut(s) 111, 151
Lsp1109I GCAGC 4 cut(s) 283, 310, 371, 922
LweI GCATC 3 cut(s) 811, 1179, 1246
MaeI CTAG 2 cut(s) 1058, 1197
MaeIII GTNAC 3 cut(s) 121, 719, 793
MalI GATC 4 cut(s) 376, 512, 542, 1265
MboI GATC 4 cut(s) 374, 510, 540, 1263
MfeI CAATTG 1 cut(s) 661
MflI RGATCY 2 cut(s) 374, 540
MhlI GDGCHC 2 cut(s) 627, 1117
MlyI GAGTC 4 cut(s) 38, 202, 435, 1052
MmeI TCCRAC 2 cut(s) 59, 168
MseI TTAA 4 cut(s) 603, 669, 836, 1334
MslI CAYNNNNRTG 2 cut(s) 165, 1314
MspI CCGG 1 cut(s) 1013
MunI CAATTG 1 cut(s) 661
MwoI GCNNNNNNNGC 3 cut(s) 350, 359, 529
NdeI CATATG 1 cut(s) 802
NdeII GATC 4 cut(s) 374, 510, 540, 1263
NlaIII CATG 7 cut(s) 719, 760, 1034, 1050, 1139, 1231, 1313
NlaIV GGNNCC 4 cut(s) 153, 407, 542, 625
NspI RCATGY 1 cut(s) 760
PaeR7I CTCGAG 1 cut(s) 233
PfeI GAWTC 1 cut(s) 943
PkrI GCNGC 5 cut(s) 156, 273, 325, 361, 912
PleI GAGTC 4 cut(s) 37, 202, 434, 1051
PpsI GAGTC 4 cut(s) 37, 202, 434, 1051
PshBI ATTAAT 1 cut(s) 669
PspN4I GGNNCC 4 cut(s) 153, 407, 542, 625
PspOMI GGGCCC 1 cut(s) 623
PspPI GGNCC 3 cut(s) 623, 624, 773
PstI CTGCAG 1 cut(s) 969
PsuI RGATCY 2 cut(s) 374, 540
RsaI GTAC 2 cut(s) 453, 923
RsaNI GTAC 2 cut(s) 452, 922
RseI CAYNNNNRTG 2 cut(s) 165, 1314
SaqAI TTAA 4 cut(s) 603, 669, 836, 1334
SatI GCNGC 5 cut(s) 155, 272, 324, 360, 911
Sau3AI GATC 4 cut(s) 374, 510, 540, 1263
Sau96I GGNCC 3 cut(s) 623, 624, 773
SchI GAGTC 4 cut(s) 38, 202, 435, 1052
SduI GDGCHC 2 cut(s) 627, 1117
SfaNI GCATC 3 cut(s) 811, 1179, 1246
SfcI CTRYAG 2 cut(s) 930, 965
Sfr274I CTCGAG 1 cut(s) 233
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 2 cut(s) 165, 1314
SmlI CTYRAG 3 cut(s) 47, 233, 1116
SmoI CTYRAG 3 cut(s) 47, 233, 1116
SsiI CCGC 2 cut(s) 26, 155
SspMI CTAG 2 cut(s) 1058, 1197
StyI CCWWGG 2 cut(s) 1057, 1196
TaqI TCGA 3 cut(s) 111, 234, 429
TaqII GACCGA 1 cut(s) 29
TatI WGTACW 1 cut(s) 921
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 1 cut(s) 943
Tru1I TTAA 4 cut(s) 603, 669, 836, 1334
Tru9I TTAA 4 cut(s) 603, 669, 836, 1334
TscAI CASTG 1 cut(s) 1281
TseI GCWGC 4 cut(s) 271, 323, 359, 910
TspDTI ATGAA 7 cut(s) 687, 713, 819, 885, 1047, 1100, 1184
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 1 cut(s) 1281
VspI ATTAAT 1 cut(s) 669
XagI CCTNNNNNAGG 1 cut(s) 413
XapI RAATTY 2 cut(s) 675, 1037
XceI RCATGY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XhoI CTCGAG 1 cut(s) 233
XmaJI CCTAGG 2 cut(s) 1057, 1196
XspI CTAG 2 cut(s) 1058, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.