MD02G1149400.v1.1

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003343
Physical Location & Seq
Reverse (-)
12305226 .. 12311087
5862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1149400.v1.1.491

Sequence Viewer

Length: 2079 bp
ATGGGAAAGGCACCAGAGCAGTTGCCCCACCTTAATGACACTCAGTATAACAAGTTGGATGAGCTCCTTACGCAAACTCAGCTGTACTCGCAGTTTTTGCTTGAGAAAATGGATAACATCACACTTGTTGGAGCCAATCAACCGAGTGAAACTGTTGAGGAAAAGAATGTTGAGGAAAAGAAAGGTCGTGGTAGGAAAAGAAAGGCAGCTGCCAATTTTGATAATAGGAAGGCAAAGAAGGCAGTTGAAGCTATGCTTACAAGATCTAAAGAGGGTGTGAAAATTGAAGATGAGAACCTCACTGAAGAACAAAGAATTGAAAAAGAACAAAAGGAACTTGTGCCTCTGCTGACTGGTGGAAAATTGAAGTCTTATCAAATCAAAGGTGTAAAGTGGTTGATCTCATTATGGCAAAATGGTCTGAATGGGATCCTTGCAGATCAAATGGGACTTGGAAAAACTATCCAAACCATTGGTTTTCTTGCTCATCTAAAAGGGAATGGAATGGATGGGCCCTACTTAGTGATTGCTCCTCTTTCTACTCTTGCGAATTGGGTAAATGAAATTTCAAGGTTCACACCTTCAATAAATGCTATTGTCTACCATGGTGACAAGAAACAAAGGGATGAGATACGAAGGAAGCACATGCCTAGTAAAATTGGCCCTAAGTTCCCTATAATTGTTACTTCGTATGAAATTGCAATGGCTGATGCAAGAAGATGTTTAAGACATTACAGTTGGAAATATCTGGTGGTTGATGAAGGTCACAGATTGAAGAACTCAAAGTGCATGCTGCTGCGGGAATTGAAACATTTACGTGTAGACAATAAGATTCTGTTGACCGGGACACCTCTACAGAATAATTTGGCAGAGCTTTGGTCATTATTAAACTTCATTTTGCCTGATATATTCTCATCCCATGAAGAATTTGAGTCATGGTTTGATTTGGCAGGAAAGTGCAGTAATGAGGCAATGAAGGAAGAATTGGAAGAGAAGAGAAGGGCTCAAATGGTTGCAAAACTCCATGCCATCTTGCGTCCTTTTCTTCTTCGAAGAATGAAGTCTGATGTTGAGCACATGCTTCCTAGGAAAAAGGAAATCATATTATATGCATGCATGACAGAGCATCAAAAACATTTTCAAGATCTTCTGATCAATAAGACATTGGAGAATTATTTAGTTGAGAGGGGAGACCATGTTCGCGGTATGAAAGGAAAGCTTAACAATCTGATGGTCCAACTTCGGAAGAACTGCAATCATCCTGACCTTTTGGAGTCTGCGTTTGATGGCTCATATTTCTACCCGCCTGTTGAACAGATTGTTGAACAGTGTGGAAAATTTAGTTTGCTGGACCGGCTGTTAAAGTACCTGTTTGCCCGCAAACACAAGGTTCTTATCTTCTCTCAGTGGACTAAGATTTTGGATATTATGGACTACTATTTTAGTGAGAAAGGATTAGAAGTTTGTAGAATTGACGGCAGTGTGAGGCTTGAAGATCGGAGAAATCAGATTGCAGCGTTCAATGACATGGACAGCAATTATAGAATCTTTCTTCTAAGCACCAGAGCTGGTGGACTGGGTATCAACCTTACTGCAGCTGATACTTGTATACTGTACGACAGTGATTGGAACCCTCAAATGGATTTGCAAGCCATGGATAGATGTCACAGAATTGGTCAAACGAAGCCTGTTCATGTTTACAGGTTGGCAACAGCACAATCTGTCGAGGGTCGGATTCTGAAAAGAGCTTTTAGCAAGTTGAAGCTTGAACATGTGGTGATTGGGAAAGGGCAGTTCCATCAAGATAAAGCGAAGAGTAGTACTGATTTCCTGGAGGAAGAGGATCTTATAGCATTGCTCCGAGAGGAAGAATCTGCTGAAGACAAGATGATACAGACGGTTATCAGTGACGAAGATTTGGAGAGGGTGTTGGATCGCAGTGATCTAATGGGCAGTCCTGCTGATCATGAAGAAGAGAAGGCCAATGGTGTTGCCGATGTGCTTCCCCTCAAAGGCCCGGGTTGGGAGGTGGTGCTACCTACTGCAGGTGGAGGCATGCTCTCCACCCTCACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

693

Amino Acids

79.27

Weight (kDa)

7.93

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 121 - 284 1.1e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 125 - 423 5.8e-85 SNF2-related domain
DEAD PF00270 139 - 288 3.5e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 372 - 581 9.1e-06 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 446 - 559 1.1e-25 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2036
Acc36I ACCTGC 1 cut(s) 2036
AccB1I GGYRCC 1 cut(s) 10
AccI GTMKAC 3 cut(s) 600, 822, 1609
AccII CGCG 1 cut(s) 1203
AciI CCGC 4 cut(s) 799, 1203, 1304, 1378
AclWI GGATC 4 cut(s) 424, 437, 1851, 1941
AcsI RAATTY 3 cut(s) 564, 926, 1337
AcuI CTGAAG 2 cut(s) 324, 1899
AfaI GTAC 4 cut(s) 86, 1367, 1616, 1822
AfiI CCNNNNNNNGG 4 cut(s) 1639, 2012, 2023, 2045
AflIII ACRYGT 2 cut(s) 817, 1771
AhlI ACTAGT 1 cut(s) 2072
AjnI CCWGG 1 cut(s) 1830
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AloI GAACNNNNNNTCC 2 cut(s) 1182, 1214
Alw21I GWGCWC 2 cut(s) 66, 1077
Alw26I GTCTC 1 cut(s) 1185
AlwI GGATC 4 cut(s) 424, 437, 1851, 1941
Ama87I CYCGRG 1 cut(s) 2017
AoxI GGCC 4 cut(s) 512, 661, 1980, 2014
ApaI GGGCCC 1 cut(s) 516
ApeKI GCWGC 6 cut(s) 206, 209, 793, 796, 1514, 1595
ApoI RAATTY 3 cut(s) 564, 926, 1337
ArsI GACNNNNNNTTYG 2 cut(s) 1402, 1434
AspA2I CCTAGG 1 cut(s) 1085
AspS9I GGNCC 6 cut(s) 512, 513, 662, 1234, 1351, 2015
AsuC2I CCSGG 3 cut(s) 844, 2018, 2019
AsuHPI GGTGA 2 cut(s) 620, 1789
AsuII TTCGAA 1 cut(s) 1051
AvaI CYCGRG 1 cut(s) 2017
AvaII GGWCC 2 cut(s) 1234, 1351
AvrII CCTAGG 1 cut(s) 1085
BaeGI GKGCMC 1 cut(s) 516
BamHI GGATCC 1 cut(s) 429
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 3 cut(s) 66, 516, 1006
BbsI GAAGAC 1 cut(s) 1887
Bbv12I GWGCWC 2 cut(s) 66, 1077
BbvI GCAGC 6 cut(s) 196, 218, 780, 783, 1526, 1607
BccI CCATC 5 cut(s) 503, 1037, 1225, 1280, 1806
BceAI ACGGC 1 cut(s) 1492
BciT130I CCWGG 1 cut(s) 1832
BclI TGATCA 2 cut(s) 1152, 1963
BcnI CCSGG 3 cut(s) 844, 2018, 2019
BcoDI GTCTC 1 cut(s) 1185
BcuI ACTAGT 1 cut(s) 2072
BfaI CTAG 3 cut(s) 651, 1086, 2073
BfmI CTRYAG 3 cut(s) 854, 1593, 2043
BfuAI ACCTGC 1 cut(s) 2036
BglII AGATCT 2 cut(s) 263, 1144
BisI GCNGC 6 cut(s) 207, 210, 794, 797, 1515, 1596
BlnI CCTAGG 1 cut(s) 1085
BlsI GCNGC 6 cut(s) 208, 211, 795, 798, 1516, 1597
BmcAI AGTACT 1 cut(s) 1822
Bme1390I CCNGG 4 cut(s) 844, 1832, 2018, 2019
Bme18I GGWCC 2 cut(s) 1234, 1351
BmeT110I CYCGRG 1 cut(s) 2017
BmgT120I GGNCC 6 cut(s) 512, 513, 662, 1234, 1351, 2015
BmiI GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
BmrFI CCNGG 4 cut(s) 844, 1832, 2018, 2019
BmrI ACTGGG 1 cut(s) 1586
BmsI GCATC 2 cut(s) 700, 1135
BmuI ACTGGG 1 cut(s) 1586
BpiI GAAGAC 1 cut(s) 1887
BplI GAGNNNNNCTC 4 cut(s) 988, 1020, 2043, 2075
BpmI CTGGAG 1 cut(s) 1853
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 3 cut(s) 844, 2018, 2019
BsaAI YACGTR 1 cut(s) 818
BsaI GGTCTC 1 cut(s) 1185
BsaJI CCNNGG 4 cut(s) 604, 1085, 1653, 2017
BsaXI ACNNNNNCTCC 6 cut(s) 1182, 1212, 1265, 1295, 1913, 1943
Bsc4I CCNNNNNNNGG 4 cut(s) 1639, 2012, 2023, 2045
Bse118I RCCGGY 1 cut(s) 1353
Bse1I ACTGG 2 cut(s) 358, 1581
Bse3DI GCAATG 3 cut(s) 708, 978, 1853
BseBI CCWGG 1 cut(s) 1832
BseDI CCNNGG 4 cut(s) 604, 1085, 1653, 2017
BseGI GGATG 5 cut(s) 64, 514, 631, 914, 1258
BseLI CCNNNNNNNGG 4 cut(s) 1639, 2012, 2023, 2045
BseMI GCAATG 3 cut(s) 708, 978, 1853
BseMII CTCAG 3 cut(s) 56, 92, 1418
BseNI ACTGG 2 cut(s) 358, 1581
BseRI GAGGAG 1 cut(s) 522
BseSI GKGCMC 1 cut(s) 516
BseXI GCAGC 6 cut(s) 196, 218, 780, 783, 1526, 1607
BsgI GTGCAG 1 cut(s) 979
Bsh1236I CGCG 1 cut(s) 1203
BshFI GGCC 4 cut(s) 514, 663, 1982, 2016
BshNI GGYRCC 1 cut(s) 10
BsiHKAI GWGCWC 2 cut(s) 66, 1077
BsiHKCI CYCGRG 1 cut(s) 2017
BsiSI CCGG 3 cut(s) 843, 1354, 2018
BslFI GGGAC 2 cut(s) 462, 859
BslI CCNNNNNNNGG 4 cut(s) 1639, 2012, 2023, 2045
BsmAI GTCTC 1 cut(s) 1185
BsmFI GGGAC 2 cut(s) 462, 859
BsnI GGCC 4 cut(s) 514, 663, 1982, 2016
Bso31I GGTCTC 1 cut(s) 1185
BsoBI CYCGRG 1 cut(s) 2017
Bsp119I TTCGAA 1 cut(s) 1051
Bsp120I GGGCCC 1 cut(s) 512
Bsp1286I GDGCHC 4 cut(s) 66, 516, 1006, 1077
Bsp19I CCATGG 2 cut(s) 604, 1653
BspACI CCGC 4 cut(s) 799, 1203, 1304, 1378
BspANI GGCC 4 cut(s) 514, 663, 1982, 2016
BspCNI CTCAG 3 cut(s) 55, 91, 1417
BspFNI CGCG 1 cut(s) 1203
BspHI TCATGA 1 cut(s) 1966
BspLI GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
BspMAI CTGCAG 2 cut(s) 1597, 2047
BspMI ACCTGC 1 cut(s) 2036
BspPI GGATC 4 cut(s) 424, 437, 1851, 1941
BspT104I TTCGAA 1 cut(s) 1051
BspT107I GGYRCC 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 1185
BsrDI GCAATG 3 cut(s) 708, 978, 1853
BsrFI RCCGGY 1 cut(s) 1353
BsrI ACTGG 2 cut(s) 358, 1581
BssAI RCCGGY 1 cut(s) 1353
BssECI CCNNGG 4 cut(s) 604, 1085, 1653, 2017
BssNAI GTATAC 1 cut(s) 1610
BssT1I CCWWGG 3 cut(s) 604, 1085, 1653
Bst1107I GTATAC 1 cut(s) 1610
Bst2UI CCWGG 1 cut(s) 1832
Bst4CI ACNGT 6 cut(s) 154, 737, 1329, 1614, 1622, 1900
Bst6I CTCTTC 5 cut(s) 984, 989, 1808, 1833, 1968
BstAPI GCANNNNNTGC 1 cut(s) 97
BstBAI YACGTR 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 5 cut(s) 791, 1114, 1378, 1650, 2057
BstDEI CTNAG 7 cut(s) 42, 78, 520, 666, 1404, 1413, 1556
BstDSI CCRYGG 2 cut(s) 604, 1653
BstENI CCTNNNNNAGG 1 cut(s) 2043
BstF5I GGATG 5 cut(s) 64, 514, 631, 914, 1258
BstFNI CGCG 1 cut(s) 1203
BstMAI GTCTC 1 cut(s) 1185
BstMWI GCNNNNNNNGC 7 cut(s) 70, 79, 88, 97, 239, 248, 1354
BstNI CCWGG 1 cut(s) 1832
BstNSI RCATGY 6 cut(s) 649, 793, 1081, 1116, 1775, 2059
BstSCI CCNGG 4 cut(s) 842, 1830, 2016, 2017
BstSFI CTRYAG 3 cut(s) 854, 1593, 2043
BstSLI GKGCMC 1 cut(s) 516
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 6 cut(s) 196, 218, 780, 783, 1526, 1607
BstV2I GAAGAC 1 cut(s) 1887
BstX2I RGATCY 4 cut(s) 263, 429, 1144, 1843
BstXI CCANNNNNNTGG 1 cut(s) 473
BstYI RGATCY 4 cut(s) 263, 429, 1144, 1843
BstZ17I GTATAC 1 cut(s) 1610
BsuRI GGCC 4 cut(s) 514, 663, 1982, 2016
BtgI CCRYGG 2 cut(s) 604, 1653
BtsCI GGATG 5 cut(s) 64, 514, 631, 914, 1258
BtsI GCAGTG 2 cut(s) 1486, 1945
BtsIMutI CAGTG 7 cut(s) 300, 1334, 1412, 1486, 1627, 1912, 1945
BveI ACCTGC 1 cut(s) 2036
Cac8I GCNNGC 5 cut(s) 791, 1114, 1378, 1650, 2057
CciI TCATGA 1 cut(s) 1966
Cfr10I RCCGGY 1 cut(s) 1353
Cfr13I GGNCC 6 cut(s) 512, 513, 662, 1234, 1351, 2015
Cfr9I CCCGGG 1 cut(s) 2017
CseI GACGC 1 cut(s) 1025
Csp6I GTAC 4 cut(s) 85, 1366, 1615, 1821
CviQI GTAC 4 cut(s) 85, 1366, 1615, 1821
DdeI CTNAG 7 cut(s) 42, 78, 520, 666, 1404, 1413, 1556
Eam1104I CTCTTC 5 cut(s) 984, 989, 1808, 1833, 1968
EarI CTCTTC 5 cut(s) 984, 989, 1808, 1833, 1968
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 604, 1085, 1653
Eco24I GRGCYC 3 cut(s) 66, 516, 1006
Eco31I GGTCTC 1 cut(s) 1185
Eco47I GGWCC 2 cut(s) 1234, 1351
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 324, 1899
Eco88I CYCGRG 1 cut(s) 2017
EcoICRI GAGCTC 1 cut(s) 64
EcoNI CCTNNNNNAGG 1 cut(s) 2043
EcoO109I RGGNCCY 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 1830
EcoT14I CCWWGG 3 cut(s) 604, 1085, 1653
EcoT22I ATGCAT 2 cut(s) 1114, 1118
EcoT38I GRGCYC 3 cut(s) 66, 516, 1006
ErhI CCWWGG 3 cut(s) 604, 1085, 1653
FalI AAGNNNNNCTT 6 cut(s) 240, 272, 1203, 1235, 1830, 1862
FaqI GGGAC 2 cut(s) 462, 859
FauI CCCGC 3 cut(s) 792, 1311, 1385
FbaI TGATCA 2 cut(s) 1152, 1963
FblI GTMKAC 3 cut(s) 600, 822, 1609
Fnu4HI GCNGC 6 cut(s) 207, 210, 794, 797, 1515, 1596
FokI GGATG 5 cut(s) 71, 521, 638, 901, 1245
FriOI GRGCYC 3 cut(s) 66, 516, 1006
Fsp4HI GCNGC 6 cut(s) 207, 210, 794, 797, 1515, 1596
FspBI CTAG 3 cut(s) 651, 1086, 2073
GluI GCNGC 6 cut(s) 207, 210, 794, 797, 1515, 1596
GsuI CTGGAG 1 cut(s) 1853
HaeIII GGCC 4 cut(s) 514, 663, 1982, 2016
HapII CCGG 3 cut(s) 843, 1354, 2018
HgaI GACGC 1 cut(s) 1025
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HindIII AAGCTT 2 cut(s) 1217, 1763
HinfI GANTC 6 cut(s) 832, 932, 1274, 1545, 1735, 1871
HpaII CCGG 3 cut(s) 843, 1354, 2018
HphI GGTGA 2 cut(s) 620, 1789
Hpy166II GTNNAC 8 cut(s) 576, 601, 823, 840, 1410, 1574, 1610, 1699
Hpy188III TCNNGA 4 cut(s) 1142, 1262, 1802, 1967
Hpy8I GTNNAC 8 cut(s) 576, 601, 823, 840, 1410, 1574, 1610, 1699
HpyAV CCTTC 8 cut(s) 223, 232, 591, 630, 755, 970, 993, 1972
HpyCH4III ACNGT 6 cut(s) 154, 737, 1329, 1614, 1622, 1900
HpyCH4IV ACGT 1 cut(s) 817
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 79, 88, 97, 239, 248, 1354
HpyF3I CTNAG 7 cut(s) 42, 78, 520, 666, 1404, 1413, 1556
HpySE526I ACGT 1 cut(s) 817
Ksp22I TGATCA 2 cut(s) 1152, 1963
LmnI GCTCC 4 cut(s) 69, 131, 535, 1863
Lsp1109I GCAGC 6 cut(s) 196, 218, 780, 783, 1526, 1607
LweI GCATC 2 cut(s) 700, 1135
MaeI CTAG 3 cut(s) 651, 1086, 2073
MaeII ACGT 1 cut(s) 817
MaeIII GTNAC 5 cut(s) 608, 682, 764, 1664, 1907
MflI RGATCY 4 cut(s) 263, 429, 1144, 1843
MhlI GDGCHC 4 cut(s) 66, 516, 1006, 1077
MlyI GAGTC 2 cut(s) 941, 1283
MmeI TCCRAC 6 cut(s) 36, 109, 719, 1261, 1712, 1911
Mph1103I ATGCAT 2 cut(s) 1114, 1118
MseI TTAA 6 cut(s) 33, 725, 887, 1221, 1361, 2077
MslI CAYNNNNRTG 2 cut(s) 33, 816
MspA1I CMGCKG 3 cut(s) 82, 209, 1598
MspI CCGG 3 cut(s) 843, 1354, 2018
MspR9I CCNGG 4 cut(s) 844, 1832, 2018, 2019
MvaI CCWGG 1 cut(s) 1832
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 7 cut(s) 70, 79, 88, 97, 239, 248, 1354
NciI CCSGG 3 cut(s) 844, 2018, 2019
NcoI CCATGG 2 cut(s) 604, 1653
NlaIV GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
NmuCI GTSAC 4 cut(s) 608, 764, 1664, 1907
NsiI ATGCAT 2 cut(s) 1114, 1118
NspI RCATGY 6 cut(s) 649, 793, 1081, 1116, 1775, 2059
NspV TTCGAA 1 cut(s) 1051
PaeI GCATGC 3 cut(s) 793, 1116, 2059
PagI TCATGA 1 cut(s) 1966
PaqCI CACCTGC 1 cut(s) 2036
PciI ACATGT 1 cut(s) 1771
PfeI GAWTC 4 cut(s) 832, 1545, 1735, 1871
PfoI TCCNGGA 1 cut(s) 1830
PkrI GCNGC 6 cut(s) 208, 211, 795, 798, 1516, 1597
PleI GAGTC 2 cut(s) 940, 1282
PpsI GAGTC 2 cut(s) 940, 1282
Ppu21I YACGTR 1 cut(s) 818
PscI ACATGT 1 cut(s) 1771
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 1830
PspGI CCWGG 1 cut(s) 1830
PspN4I GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
PspOMI GGGCCC 1 cut(s) 512
PspPI GGNCC 6 cut(s) 512, 513, 662, 1234, 1351, 2015
PstI CTGCAG 2 cut(s) 1597, 2047
PsuI RGATCY 4 cut(s) 263, 429, 1144, 1843
PvuII CAGCTG 3 cut(s) 82, 209, 1598
RsaI GTAC 4 cut(s) 86, 1367, 1616, 1822
RsaNI GTAC 4 cut(s) 85, 1366, 1615, 1821
RseI CAYNNNNRTG 2 cut(s) 33, 816
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 6 cut(s) 33, 725, 887, 1221, 1361, 2077
SatI GCNGC 6 cut(s) 207, 210, 794, 797, 1515, 1596
Sau96I GGNCC 6 cut(s) 512, 513, 662, 1234, 1351, 2015
ScaI AGTACT 1 cut(s) 1822
SchI GAGTC 2 cut(s) 941, 1283
ScrFI CCNGG 4 cut(s) 844, 1832, 2018, 2019
SduI GDGCHC 4 cut(s) 66, 516, 1006, 1077
SfaNI GCATC 2 cut(s) 700, 1135
SfcI CTRYAG 3 cut(s) 854, 1593, 2043
SfuI TTCGAA 1 cut(s) 1051
SinI GGWCC 2 cut(s) 1234, 1351
SmaI CCCGGG 1 cut(s) 2019
SmiMI CAYNNNNRTG 2 cut(s) 33, 816
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SpeI ACTAGT 1 cut(s) 2072
SphI GCATGC 3 cut(s) 793, 1116, 2059
SsiI CCGC 4 cut(s) 799, 1203, 1304, 1378
SspMI CTAG 3 cut(s) 651, 1086, 2073
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 4 cut(s) 842, 1830, 2016, 2017
StyI CCWWGG 3 cut(s) 604, 1085, 1653
TaaI ACNGT 6 cut(s) 154, 737, 1329, 1614, 1622, 1900
TaiI ACGT 1 cut(s) 820
TaqI TCGA 2 cut(s) 1051, 1725
TatI WGTACW 2 cut(s) 84, 1820
TfiI GAWTC 4 cut(s) 832, 1545, 1735, 1871
Tru1I TTAA 6 cut(s) 33, 725, 887, 1221, 1361, 2077
Tru9I TTAA 6 cut(s) 33, 725, 887, 1221, 1361, 2077
TscAI CASTG 7 cut(s) 307, 1334, 1412, 1486, 1627, 1912, 1945
TseFI GTSAC 4 cut(s) 608, 764, 1664, 1907
TseI GCWGC 6 cut(s) 206, 209, 793, 796, 1514, 1595
Tsp45I GTSAC 4 cut(s) 608, 764, 1664, 1907
TspMI CCCGGG 1 cut(s) 2017
TspRI CASTG 7 cut(s) 307, 1334, 1412, 1486, 1627, 1912, 1945
VpaK11BI GGWCC 2 cut(s) 1234, 1351
XagI CCTNNNNNAGG 1 cut(s) 2043
XapI RAATTY 3 cut(s) 564, 926, 1337
XceI RCATGY 6 cut(s) 649, 793, 1081, 1116, 1775, 2059
XmaI CCCGGG 1 cut(s) 2017
XmaJI CCTAGG 1 cut(s) 1085
XmiI GTMKAC 3 cut(s) 600, 822, 1609
XspI CTAG 3 cut(s) 651, 1086, 2073
ZrmI AGTACT 1 cut(s) 1822
Zsp2I ATGCAT 2 cut(s) 1114, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.