AT5G67490

Protein of unknown function (DUF1674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005760
Physical Location & Seq
Reverse (-)
26934153 .. 26934952
800 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67490.1

Sequence Viewer

Length: 327 bp
ATGGCGACGAACAACATCGTACGTTTGATCGCTCCAAGCCGATCCGTCTCCTCTAGATTCCTCGAGCCAGTGTCACGGTTTCTAAGCTCCGGTACACCTCCTCCGCCGCAAGCTCCATCTCCGAATCAGGATCTGAACCGGGACGTGAAGCCAGATCAGAATCTTCAACAGAATCTGCAGATGCAGAAGGAAGAAGAAGAGGAAGGAGAAGGTGGTGGAGGTGGAGAGTTTGTGAACGAAGATACTGGTGAGATTGGAGGGCCTAGAGGTCCTGAACCGACTCGGTACGGCGATTGGGAACAACGTGGTCGATGCTCTGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1674

Protein Analysis

108

Amino Acids

11.87

Weight (kDa)

4.53

Isoelectric Point (pI)

71.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1674 PF07896 78 - 108 2.6e-16 Protein of unknown function (DUF1674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 104, 107
AclWI GGATC 2 cut(s) 36, 138
AfaI GTAC 3 cut(s) 21, 94, 287
AgsI TTSAA 1 cut(s) 167
AjiI CACGTC 1 cut(s) 145
AluBI AGCT 2 cut(s) 87, 113
AluI AGCT 2 cut(s) 87, 113
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 2 cut(s) 36, 138
AlwNI CAGNNNCTG 2 cut(s) 133, 175
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 260
AspS9I GGNCC 2 cut(s) 260, 269
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 62
AvaII GGWCC 1 cut(s) 269
BccI CCATC 1 cut(s) 124
BceAI ACGGC 1 cut(s) 304
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 2 cut(s) 54, 264
BfmI CTRYAG 1 cut(s) 176
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 2 cut(s) 260, 269
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 171, 302
BpuMI CCSGG 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 159
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 216, 246
Bse1I ACTGG 2 cut(s) 68, 250
Bse8I GATNNNNATC 1 cut(s) 159
BseJI GATNNNNATC 1 cut(s) 159
BseNI ACTGG 2 cut(s) 68, 250
BseRI GAGGAG 2 cut(s) 40, 90
BshFI GGCC 1 cut(s) 262
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 90, 139
BsiWI CGTACG 1 cut(s) 19
BslFI GGGAC 1 cut(s) 155
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 62
Bsp143I GATC 4 cut(s) 27, 41, 130, 154
BspACI CCGC 2 cut(s) 104, 107
BspANI GGCC 1 cut(s) 262
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 2 cut(s) 36, 138
BsrI ACTGG 2 cut(s) 68, 250
BssMI GATC 4 cut(s) 27, 41, 130, 154
Bst4CI ACNGT 1 cut(s) 78
Bst6I CTCTTC 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 1 cut(s) 83
BstKTI GATC 4 cut(s) 30, 44, 133, 157
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 4 cut(s) 27, 41, 130, 154
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 176
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
BsuRI GGCC 1 cut(s) 262
BtrI CACGTC 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 111
CaiI CAGNNNCTG 2 cut(s) 133, 175
Cfr13I GGNCC 2 cut(s) 260, 269
Csp6I GTAC 3 cut(s) 20, 93, 286
CviJI RGCY 6 cut(s) 39, 67, 87, 113, 151, 262
CviKI_1 RGCY 6 cut(s) 39, 67, 87, 113, 151, 262
CviQI GTAC 3 cut(s) 20, 93, 286
DdeI CTNAG 1 cut(s) 83
DpnI GATC 4 cut(s) 29, 43, 132, 156
DpnII GATC 4 cut(s) 27, 41, 130, 154
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
EciI GGCGGA 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 62
EcoO109I RGGNCCY 2 cut(s) 260, 269
Esp3I CGTCTC 1 cut(s) 52
FaqI GGGAC 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 107
FspBI CTAG 2 cut(s) 54, 264
GluI GCNGC 1 cut(s) 107
HaeIII GGCC 1 cut(s) 262
HapII CCGG 2 cut(s) 90, 139
HinfI GANTC 5 cut(s) 57, 124, 160, 172, 280
HpaII CCGG 2 cut(s) 90, 139
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 95, 235
Hpy188I TCNGA 5 cut(s) 123, 135, 159, 319, 326
Hpy188III TCNNGA 3 cut(s) 54, 128, 272
Hpy8I GTNNAC 2 cut(s) 95, 235
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 181, 197, 203
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 3 cut(s) 22, 144, 304
HpyCH4V TGCA 2 cut(s) 178, 184
HpyF3I CTNAG 1 cut(s) 83
HpySE526I ACGT 3 cut(s) 22, 144, 304
Kzo9I GATC 4 cut(s) 27, 41, 130, 154
LmnI GCTCC 3 cut(s) 37, 92, 118
LpnPI CCDG 7 cut(s) 81, 103, 113, 152, 165, 231, 285
LweI GCATC 2 cut(s) 171, 302
MaeI CTAG 2 cut(s) 54, 264
MaeII ACGT 3 cut(s) 22, 144, 304
MaeIII GTNAC 1 cut(s) 72
MalI GATC 4 cut(s) 29, 43, 132, 156
MboI GATC 4 cut(s) 27, 41, 130, 154
MboII GAAGA 5 cut(s) 155, 203, 206, 209, 251
MflI RGATCY 1 cut(s) 130
MlyI GAGTC 1 cut(s) 274
MnlI CCTC 8 cut(s) 61, 71, 108, 111, 193, 212, 251, 260
MspI CCGG 2 cut(s) 90, 139
MspR9I CCNGG 1 cut(s) 140
NciI CCSGG 1 cut(s) 140
NdeII GATC 4 cut(s) 27, 41, 130, 154
NmuCI GTSAC 1 cut(s) 72
PaeR7I CTCGAG 1 cut(s) 62
PfeI GAWTC 4 cut(s) 57, 124, 160, 172
Pfl23II CGTACG 1 cut(s) 19
PkrI GCNGC 1 cut(s) 108
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PpuMI RGGWCCY 1 cut(s) 269
Psp5II RGGWCCY 1 cut(s) 269
PspLI CGTACG 1 cut(s) 19
PspPI GGNCC 2 cut(s) 260, 269
PspPPI RGGWCCY 1 cut(s) 269
PspXI VCTCGAGB 1 cut(s) 62
PstI CTGCAG 1 cut(s) 180
PstNI CAGNNNCTG 2 cut(s) 133, 175
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 3 cut(s) 21, 94, 287
RsaNI GTAC 3 cut(s) 20, 93, 286
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 4 cut(s) 27, 41, 130, 154
Sau96I GGNCC 2 cut(s) 260, 269
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 1 cut(s) 140
SetI ASST 9 cut(s) 25, 89, 100, 115, 147, 214, 223, 271, 307
SfaNI GCATC 2 cut(s) 171, 302
SfcI CTRYAG 1 cut(s) 176
Sfr274I CTCGAG 1 cut(s) 62
SinI GGWCC 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SsiI CCGC 2 cut(s) 104, 107
SspMI CTAG 2 cut(s) 54, 264
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 3 cut(s) 25, 147, 307
TaqI TCGA 2 cut(s) 63, 310
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 4 cut(s) 57, 124, 160, 172
TscAI CASTG 1 cut(s) 75
TseFI GTSAC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 75
VpaK11BI GGWCC 1 cut(s) 269
XbaI TCTAGA 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 62
XspI CTAG 2 cut(s) 54, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.