RchiOBHm_Chr1g0381021

UPF0369 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005760
Physical Location & Seq
Forward (+)
66429180 .. 66430646
1467 bp
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UTR
Exon/CDS
Intron
PRQ60422

Sequence Viewer

Length: 405 bp
ATGGCGACGAATCTGAGCCGTCGATTCTCATCAACGATCGTAACGAAAGCCGAGCCTCTGGCTCGCTCAGCTGTCAACTCAGTGGGCCGGCTCTTGTCCTCCTCGACCGGGCAATCTCAACCGGAGCGCGAAAACGCGGAGGTGAAATACGACGGAAGCGCCAAACAGGACGACGATGGAGCTGAGGATGGTGAAAATGACGGAGATGAGTTGGACTTGAATAAAGAGACGGGTGAGATCGGCGGGCCGAAAGGGCCCGAGCCGACCCGGTACGGCGACTGGGAAAAAAACGGGCTTCTTGCTGCATTGATCCATGAATTCTCAGATTTGGACGTAGAAGGAGATTCCAGAGTTCTTATTAACAGTATAATGGGATATATTCAAACTTCTTGGATAACAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1674

Protein Analysis

134

Amino Acids

14.49

Weight (kDa)

4.38

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1674 PF07896 67 - 99 2.4e-14 Protein of unknown function (DUF1674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 129, 137
AciI CCGC 2 cut(s) 137, 243
AclWI GGATC 1 cut(s) 304
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 2 cut(s) 220, 383
AluBI AGCT 2 cut(s) 71, 182
AluI AGCT 2 cut(s) 71, 182
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 1 cut(s) 304
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 3 cut(s) 85, 245, 254
ApaI GGGCCC 1 cut(s) 258
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 2 cut(s) 129, 161
AspS9I GGNCC 4 cut(s) 85, 245, 254, 255
AsuC2I CCSGG 2 cut(s) 109, 268
AsuHPI GGTGA 3 cut(s) 154, 203, 245
AvaI CYCGRG 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 258
BanII GRGCYC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 289
BccI CCATC 2 cut(s) 170, 182
BceAI ACGGC 2 cut(s) 3, 289
BcnI CCSGG 2 cut(s) 109, 268
BcoDI GTCTC 1 cut(s) 221
BfoI RGCGCY 1 cut(s) 162
BglI GCCNNNNNGGC 1 cut(s) 253
BisI GCNGC 1 cut(s) 303
BlpI GCTNAGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 2 cut(s) 109, 268
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 4 cut(s) 85, 245, 254, 255
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 109, 268
BmrI ACTGGG 1 cut(s) 289
BmuI ACTGGG 1 cut(s) 289
Bpu10I CCTNAGC 1 cut(s) 183
Bpu1102I GCTNAGC 1 cut(s) 67
BpuMI CCSGG 2 cut(s) 109, 268
BsaBI GATNNNNATC 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse118I RCCGGY 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 284
Bse8I GATNNNNATC 1 cut(s) 28
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMII CTCAG 5 cut(s) 5, 81, 93, 174, 336
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 1 cut(s) 289
Bsh1236I CGCG 2 cut(s) 129, 137
Bsh1285I CGRYCG 2 cut(s) 39, 108
BshFI GGCC 3 cut(s) 87, 247, 256
BsiEI CGRYCG 2 cut(s) 39, 108
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 4 cut(s) 88, 108, 122, 268
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 221
BsmBI CGTCTC 1 cut(s) 221
BsnI GGCC 3 cut(s) 87, 247, 256
BsoBI CYCGRG 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 3 cut(s) 36, 237, 309
Bsp1720I GCTNAGC 1 cut(s) 67
BspACI CCGC 2 cut(s) 137, 243
BspANI GGCC 3 cut(s) 87, 247, 256
BspCNI CTCAG 5 cut(s) 6, 80, 92, 175, 335
BspFNI CGCG 2 cut(s) 129, 137
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 87
BsrI ACTGG 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 87
BssMI GATC 3 cut(s) 36, 237, 309
Bst4CI ACNGT 2 cut(s) 365, 400
BstC8I GCNNGC 3 cut(s) 64, 89, 245
BstDEI CTNAG 5 cut(s) 14, 67, 79, 183, 322
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 2 cut(s) 129, 137
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 129, 161
BstKTI GATC 3 cut(s) 39, 240, 312
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 3 cut(s) 36, 237, 309
BstMCI CGRYCG 2 cut(s) 39, 108
BstMWI GCNNNNNNNGC 2 cut(s) 68, 253
BstSCI CCNGG 2 cut(s) 107, 266
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 2 cut(s) 129, 137
BstV1I GCAGC 1 cut(s) 289
BsuRI GGCC 3 cut(s) 87, 247, 256
BtsCI GGATG 1 cut(s) 193
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 3 cut(s) 64, 89, 245
CfoI GCGC 2 cut(s) 129, 161
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 4 cut(s) 85, 245, 254, 255
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 314
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 5 cut(s) 14, 67, 79, 183, 322
DpnI GATC 3 cut(s) 38, 239, 311
DpnII GATC 3 cut(s) 36, 237, 309
Eco24I GRGCYC 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 1 cut(s) 317
FaiI YATR 4 cut(s) 315, 368, 378, 403
FatI CATG 1 cut(s) 313
FauI CCCGC 1 cut(s) 236
Fnu4HI GCNGC 1 cut(s) 303
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 1 cut(s) 303
GlaI GCGC 2 cut(s) 128, 160
GluI GCNGC 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 87, 247, 256
HapII CCGG 4 cut(s) 88, 108, 122, 268
HhaI GCGC 2 cut(s) 129, 161
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 2 cut(s) 127, 159
HinP1I GCGC 2 cut(s) 127, 159
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 3 cut(s) 10, 24, 344
HpaII CCGG 4 cut(s) 88, 108, 122, 268
HphI GGTGA 3 cut(s) 154, 203, 245
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 15, 325
Hpy188III TCNNGA 1 cut(s) 348
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 4 cut(s) 10, 24, 155, 176
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 2 cut(s) 365, 400
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 253
HpyF3I CTNAG 5 cut(s) 14, 67, 79, 183, 322
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 2 cut(s) 127, 159
KroI GCCGGC 1 cut(s) 87
KroNI GCCGGC 1 cut(s) 89
Kzo9I GATC 3 cut(s) 36, 237, 309
LmnI GCTCC 2 cut(s) 124, 179
LpnPI CCDG 8 cut(s) 44, 101, 121, 135, 152, 265, 281, 361
Lsp1109I GCAGC 1 cut(s) 289
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 40
MalI GATC 3 cut(s) 38, 239, 311
MboI GATC 3 cut(s) 36, 237, 309
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 5 cut(s) 66, 109, 112, 133, 178
MroNI GCCGGC 1 cut(s) 87
MseI TTAA 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 4 cut(s) 88, 108, 122, 268
MspR9I CCNGG 2 cut(s) 109, 268
MvnI CGCG 2 cut(s) 129, 137
MwoI GCNNNNNNNGC 2 cut(s) 68, 253
NaeI GCCGGC 1 cut(s) 89
NciI CCSGG 2 cut(s) 109, 268
NdeII GATC 3 cut(s) 36, 237, 309
NgoMIV GCCGGC 1 cut(s) 87
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 1 cut(s) 256
NmeAIII GCCGAG 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PdiI GCCGGC 1 cut(s) 89
PfeI GAWTC 3 cut(s) 10, 24, 344
PkrI GCNGC 1 cut(s) 304
Ple19I CGATCG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 256
PspOMI GGGCCC 1 cut(s) 254
PspPI GGNCC 4 cut(s) 85, 245, 254, 255
PvuI CGATCG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 3 cut(s) 36, 237, 309
Sau96I GGNCC 4 cut(s) 85, 245, 254, 255
ScrFI CCNGG 2 cut(s) 109, 268
SduI GDGCHC 1 cut(s) 258
SetI ASST 4 cut(s) 73, 144, 184, 336
SfiI GGCCNNNNNGGCC 1 cut(s) 253
Sse9I AATT 1 cut(s) 317
SsiI CCGC 2 cut(s) 137, 243
StyD4I CCNGG 2 cut(s) 107, 266
TaaI ACNGT 2 cut(s) 365, 400
TaiI ACGT 1 cut(s) 336
TaqI TCGA 2 cut(s) 22, 104
TasI AATT 1 cut(s) 317
TfiI GAWTC 3 cut(s) 10, 24, 344
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TscAI CASTG 1 cut(s) 87
TseI GCWGC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 2 cut(s) 168, 216
TspRI CASTG 1 cut(s) 87
XapI RAATTY 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.