FvH4_1g06830

Deoxyhypusine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002811
Physical Location & Seq
Forward (+)
3610503 .. 3613347
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06830.t2

Sequence Viewer

Length: 1098 bp
ATGGAAGAAGACGGTAAAATCGCAAGCGTGCACTCCACAGTGTTCAAAGAATCACAGAATCTACAAGGCAAATGCACCGAAATCAACGGCTACGATTTCAACCAAGGCCTCGACTACTCCCGTCTCTTCGGTTCAATGCTCACCACCGGTTTCCAAGCCACCAATCTCGGAGAAGCCATCCAGGTCGTCAACCAAATGCTAGACTGGAGGCTGGCAGATGAGGCTGTAGCTGACGACTGCGGCGAGGAGGAGAGGGATGCGAAGTTTAGGGAGAGTGTCAAGTGTAAAGTGTTTCTGGGTTTCACTTCGAATTTGGTTTCGTCTGGGCTTAGAGATGTGTTCAGGTACTTGGTGGAGCACCATTTTGTGGATGTGGTGGTTACAACTGCTGGTGGCATTGAGGAGGATCTAATCAAGTGCCTTGCGCCGACTTATAAGGGCGATTTCGCGCTGGCGGGGGCGGAATTGAGGTCCAAAGGGCTGAATCGTATCGGTAATTTGCTGGTTCCTAATGGGAATTATTGTAAGTTTGAGGATTGGATCATACCGATTTTCGATCAGATGTTGAAGGAGCAGGTTGAGCAGAATGTGCTGTGGACGCCTTCTAAGTTGATTGCGCGCTTGGGGAAGGAAATCAATGACGAAAGATCGTATCTTTACTGGGCATACAAGAACAATGTCTCAGTTTACTGTCCATCCATAACCGATGGCTCATTGGGAGACATGTTGTACTTTCATTCTTTCCGCAGCCCAGGTCTGGTTGTTGACTTAGTGCAAGATATTAGGGCCGTGAATGGTGAAGCTGTTCACGCAGGTTTGAGGAAGACTGGAATGATCATCCTGGGAGGAGGCCTGCCCAAACATCACATTTGTAATGCCAATATGATGCGAAATGGTGCAGATTATGCTGTGTTCATAAATACTGCACAAGAGTATGATGGTAGTGATTCTGGAGCTCGTCCTGATGAGGCTGTATCATGGGGGAAAATACGGGGTTCTGCTAAAACTGTCAAGGTGCACTGCGATGCAACAATTGCTTTCCCTCTTCTGGTTGCTGAGACATTTGCTAAGAAAGTTATCAAGACTACTACAACTTAA

Protein Analysis

366

Amino Acids

40.41

Weight (kDa)

5.47

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DS PF01916 42 - 350 3.6e-123 Deoxyhypusine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 435
Acc36I ACCTGC 2 cut(s) 565, 803
AccB7I CCANNNNNTGG 1 cut(s) 367
AccII CGCG 2 cut(s) 449, 619
AciI CCGC 4 cut(s) 240, 455, 461, 745
AclWI GGATC 2 cut(s) 414, 548
AcsI RAATTY 1 cut(s) 310
AcyI GRCGYC 1 cut(s) 599
AfaI GTAC 2 cut(s) 347, 731
AfiI CCNNNNNNNGG 3 cut(s) 367, 757, 1048
AflIII ACRYGT 1 cut(s) 723
AgeI ACCGGT 1 cut(s) 146
AgsI TTSAA 4 cut(s) 46, 100, 135, 568
AjnI CCWGG 3 cut(s) 180, 751, 840
AluBI AGCT 3 cut(s) 230, 803, 956
AluI AGCT 3 cut(s) 230, 803, 956
Alw21I GWGCWC 4 cut(s) 33, 360, 958, 1020
Alw26I GTCTC 4 cut(s) 128, 685, 714, 1052
Alw44I GTGCAC 2 cut(s) 29, 1016
AlwI GGATC 2 cut(s) 414, 548
AoxI GGCC 3 cut(s) 106, 786, 850
ApaLI GTGCAC 2 cut(s) 29, 1016
ApeKI GCWGC 1 cut(s) 747
ApoI RAATTY 1 cut(s) 310
AsiGI ACCGGT 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 804
AspLEI GCGC 4 cut(s) 427, 451, 619, 621
AspS9I GGNCC 2 cut(s) 471, 786
AsuHPI GGTGA 2 cut(s) 133, 809
AsuII TTCGAA 1 cut(s) 308
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 2 cut(s) 33, 1020
BanII GRGCYC 1 cut(s) 958
BbsI GAAGAC 2 cut(s) 15, 830
Bbv12I GWGCWC 4 cut(s) 33, 360, 958, 1020
BbvI GCAGC 1 cut(s) 759
BccI CCATC 4 cut(s) 185, 701, 703, 932
BceAI ACGGC 2 cut(s) 103, 773
BciT130I CCWGG 3 cut(s) 182, 753, 842
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 4 cut(s) 128, 685, 714, 1052
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 2 cut(s) 565, 803
BisI GCNGC 2 cut(s) 241, 748
BlsI GCNGC 2 cut(s) 242, 749
Bme1390I CCNGG 3 cut(s) 182, 753, 842
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 471, 786
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 3 cut(s) 182, 753, 842
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 3 cut(s) 247, 876, 1015
BmuI ACTGGG 1 cut(s) 670
BpiI GAAGAC 2 cut(s) 15, 830
BpmI CTGGAG 2 cut(s) 226, 972
Bpu14I TTCGAA 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 599
BsaJI CCNNGG 3 cut(s) 103, 751, 841
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 757, 1048
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 3 cut(s) 209, 665, 832
BseBI CCWGG 3 cut(s) 182, 753, 842
BseDI CCNNGG 3 cut(s) 103, 751, 841
BseGI GGATG 5 cut(s) 177, 262, 376, 695, 837
BseLI CCNNNNNNNGG 3 cut(s) 367, 757, 1048
BseMII CTCAG 2 cut(s) 696, 1047
BseNI ACTGG 3 cut(s) 209, 665, 832
BsePI GCGCGC 1 cut(s) 617
BseRI GAGGAG 4 cut(s) 260, 263, 416, 861
BseSI GKGCMC 2 cut(s) 33, 1020
BseXI GCAGC 1 cut(s) 759
BsgI GTGCAG 2 cut(s) 909, 918
Bsh1236I CGCG 2 cut(s) 449, 619
BshFI GGCC 3 cut(s) 108, 788, 852
BshTI ACCGGT 1 cut(s) 146
BsiHKAI GWGCWC 4 cut(s) 33, 360, 958, 1020
BsiSI CCGG 1 cut(s) 147
BslI CCNNNNNNNGG 3 cut(s) 367, 757, 1048
BsmAI GTCTC 4 cut(s) 128, 685, 714, 1052
BsmBI CGTCTC 1 cut(s) 128
BsnI GGCC 3 cut(s) 108, 788, 852
Bsp119I TTCGAA 1 cut(s) 308
Bsp1286I GDGCHC 4 cut(s) 33, 360, 958, 1020
Bsp143I GATC 5 cut(s) 406, 540, 556, 647, 834
BspACI CCGC 4 cut(s) 240, 455, 461, 745
BspANI GGCC 3 cut(s) 108, 788, 852
BspCNI CTCAG 2 cut(s) 695, 1048
BspFNI CGCG 2 cut(s) 449, 619
BspLI GGNNCC 1 cut(s) 507
BspMI ACCTGC 2 cut(s) 565, 803
BspPI GGATC 2 cut(s) 414, 548
BspT104I TTCGAA 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 3 cut(s) 209, 665, 832
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 103, 751, 841
BssHII GCGCGC 1 cut(s) 617
BssMI GATC 5 cut(s) 406, 540, 556, 647, 834
BssNI GRCGYC 1 cut(s) 599
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 3 cut(s) 182, 753, 842
Bst4CI ACNGT 4 cut(s) 14, 40, 692, 1009
Bst6I CTCTTC 2 cut(s) 131, 1050
BstACI GRCGYC 1 cut(s) 599
BstAPI GCANNNNNTGC 3 cut(s) 589, 905, 1034
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 6 cut(s) 25, 29, 213, 453, 619, 854
BstDEI CTNAG 6 cut(s) 329, 606, 682, 769, 1056, 1068
BstF5I GGATG 5 cut(s) 177, 262, 376, 695, 837
BstFNI CGCG 2 cut(s) 449, 619
BstHHI GCGC 4 cut(s) 427, 451, 619, 621
BstKTI GATC 5 cut(s) 409, 543, 559, 650, 837
BstMAI GTCTC 4 cut(s) 128, 685, 714, 1052
BstMBI GATC 5 cut(s) 406, 540, 556, 647, 834
BstMWI GCNNNNNNNGC 7 cut(s) 221, 580, 589, 598, 809, 905, 1034
BstNI CCWGG 3 cut(s) 182, 753, 842
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 3 cut(s) 180, 751, 840
BstSFI CTRYAG 1 cut(s) 225
BstSLI GKGCMC 2 cut(s) 33, 1020
BstUI CGCG 2 cut(s) 449, 619
BstV1I GCAGC 1 cut(s) 759
BstV2I GAAGAC 2 cut(s) 15, 830
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 3 cut(s) 108, 788, 852
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 5 cut(s) 177, 262, 376, 695, 837
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 2 cut(s) 45, 1018
BveI ACCTGC 2 cut(s) 565, 803
Cac8I GCNNGC 6 cut(s) 25, 29, 213, 453, 619, 854
CfoI GCGC 4 cut(s) 427, 451, 619, 621
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 471, 786
CseI GACGC 1 cut(s) 607
Csp6I GTAC 2 cut(s) 346, 730
CspAI ACCGGT 1 cut(s) 146
CviAII CATG 2 cut(s) 724, 978
CviQI GTAC 2 cut(s) 346, 730
DdeI CTNAG 6 cut(s) 329, 606, 682, 769, 1056, 1068
DpnI GATC 5 cut(s) 408, 542, 558, 649, 836
DpnII GATC 5 cut(s) 406, 540, 556, 647, 834
Eam1104I CTCTTC 2 cut(s) 131, 1050
EarI CTCTTC 2 cut(s) 131, 1050
EciI GGCGGA 1 cut(s) 476
Ecl136II GAGCTC 1 cut(s) 956
Eco130I CCWWGG 1 cut(s) 103
Eco147I AGGCCT 2 cut(s) 108, 852
Eco24I GRGCYC 1 cut(s) 958
Eco47I GGWCC 1 cut(s) 471
Eco53kI GAGCTC 1 cut(s) 956
EcoICRI GAGCTC 1 cut(s) 956
EcoRII CCWGG 3 cut(s) 180, 751, 840
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 2 cut(s) 727, 981
FatI CATG 2 cut(s) 723, 977
FauI CCCGC 1 cut(s) 448
FbaI TGATCA 1 cut(s) 834
Fnu4HI GCNGC 2 cut(s) 241, 748
FokI GGATG 5 cut(s) 164, 269, 383, 682, 824
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 2 cut(s) 241, 748
FspBI CTAG 1 cut(s) 200
GlaI GCGC 4 cut(s) 426, 450, 618, 620
GluI GCNGC 2 cut(s) 241, 748
GsuI CTGGAG 2 cut(s) 226, 972
HaeIII GGCC 3 cut(s) 108, 788, 852
HapII CCGG 1 cut(s) 147
HgaI GACGC 1 cut(s) 607
HhaI GCGC 4 cut(s) 427, 451, 619, 621
Hin1I GRCGYC 1 cut(s) 599
Hin1II CATG 2 cut(s) 727, 981
Hin6I GCGC 4 cut(s) 425, 449, 617, 619
HinP1I GCGC 4 cut(s) 425, 449, 617, 619
HincII GTYRAC 2 cut(s) 190, 766
HindII GTYRAC 2 cut(s) 190, 766
HinfI GANTC 4 cut(s) 50, 58, 484, 947
HpaII CCGG 1 cut(s) 147
HphI GGTGA 2 cut(s) 133, 809
Hpy166II GTNNAC 7 cut(s) 31, 190, 597, 688, 766, 808, 1018
Hpy188I TCNGA 2 cut(s) 170, 561
Hpy188III TCNNGA 3 cut(s) 951, 962, 1081
Hpy8I GTNNAC 7 cut(s) 31, 190, 597, 688, 766, 808, 1018
HpyAV CCTTC 3 cut(s) 562, 612, 622
HpyCH4III ACNGT 4 cut(s) 14, 40, 692, 1009
HpyCH4V TGCA 7 cut(s) 31, 75, 775, 899, 926, 1018, 1028
HpyF10VI GCNNNNNNNGC 7 cut(s) 221, 580, 589, 598, 809, 905, 1034
HpyF3I CTNAG 6 cut(s) 329, 606, 682, 769, 1056, 1068
Hsp92I GRCGYC 1 cut(s) 599
Hsp92II CATG 2 cut(s) 727, 981
HspAI GCGC 4 cut(s) 425, 449, 617, 619
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 5 cut(s) 406, 540, 556, 647, 834
LmnI GCTCC 3 cut(s) 355, 571, 953
Lsp1109I GCAGC 1 cut(s) 759
LweI GCATC 3 cut(s) 247, 876, 1015
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 379
MalI GATC 5 cut(s) 408, 542, 558, 649, 836
MboI GATC 5 cut(s) 406, 540, 556, 647, 834
MboII GAAGA 5 cut(s) 17, 20, 118, 835, 1037
MfeI CAATTG 1 cut(s) 1032
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 4 cut(s) 33, 360, 958, 1020
MluCI AATT 5 cut(s) 310, 464, 496, 517, 1032
MroXI GAANNNNTTC 1 cut(s) 804
MseI TTAA 1 cut(s) 1096
MslI CAYNNNNRTG 1 cut(s) 1023
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 3 cut(s) 182, 753, 842
MunI CAATTG 1 cut(s) 1032
MvaI CCWGG 3 cut(s) 182, 753, 842
MvnI CGCG 2 cut(s) 449, 619
MwoI GCNNNNNNNGC 7 cut(s) 221, 580, 589, 598, 809, 905, 1034
NdeII GATC 5 cut(s) 406, 540, 556, 647, 834
NlaIII CATG 2 cut(s) 727, 981
NlaIV GGNNCC 1 cut(s) 507
NspI RCATGY 1 cut(s) 727
NspV TTCGAA 1 cut(s) 308
PauI GCGCGC 1 cut(s) 617
PceI AGGCCT 2 cut(s) 108, 852
PciI ACATGT 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 4 cut(s) 50, 58, 484, 947
PflMI CCANNNNNTGG 1 cut(s) 367
PinAI ACCGGT 1 cut(s) 146
PkrI GCNGC 2 cut(s) 242, 749
PscI ACATGT 1 cut(s) 723
PsiI TTATAA 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 958
Psp6I CCWGG 3 cut(s) 180, 751, 840
PspGI CCWGG 3 cut(s) 180, 751, 840
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 2 cut(s) 471, 786
PsuI RGATCY 1 cut(s) 406
PteI GCGCGC 1 cut(s) 617
RsaI GTAC 2 cut(s) 347, 731
RsaNI GTAC 2 cut(s) 346, 730
RseI CAYNNNNRTG 1 cut(s) 1023
SacI GAGCTC 1 cut(s) 958
SaqAI TTAA 1 cut(s) 1096
SatI GCNGC 2 cut(s) 241, 748
Sau3AI GATC 5 cut(s) 406, 540, 556, 647, 834
Sau96I GGNCC 2 cut(s) 471, 786
ScrFI CCNGG 3 cut(s) 182, 753, 842
SduI GDGCHC 4 cut(s) 33, 360, 958, 1020
SfaNI GCATC 3 cut(s) 247, 876, 1015
SfcI CTRYAG 1 cut(s) 225
SfuI TTCGAA 1 cut(s) 308
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 1023
Sse9I AATT 5 cut(s) 310, 464, 496, 517, 1032
SseBI AGGCCT 2 cut(s) 108, 852
SsiI CCGC 4 cut(s) 240, 455, 461, 745
SspMI CTAG 1 cut(s) 200
SstI GAGCTC 1 cut(s) 958
StuI AGGCCT 2 cut(s) 108, 852
StyD4I CCNGG 3 cut(s) 180, 751, 840
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 4 cut(s) 14, 40, 692, 1009
TaqI TCGA 3 cut(s) 111, 308, 555
TasI AATT 5 cut(s) 310, 464, 496, 517, 1032
TatI WGTACW 1 cut(s) 729
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 4 cut(s) 50, 58, 484, 947
Tru1I TTAA 1 cut(s) 1096
Tru9I TTAA 1 cut(s) 1096
TscAI CASTG 2 cut(s) 45, 1025
TseI GCWGC 1 cut(s) 747
TspDTI ATGAA 2 cut(s) 725, 904
TspRI CASTG 2 cut(s) 45, 1025
Van91I CCANNNNNTGG 1 cut(s) 367
VneI GTGCAC 2 cut(s) 29, 1016
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 727
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.