RLG00000016328

Deoxyhypusine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002811
Physical Location & Seq
Forward (+)
5696627 .. 5698715
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016328

Sequence Viewer

Length: 1275 bp
ATGTCTTCTTCTGTCAGTGTAGAACCTCACAACCGCCGGTTCCTTTTCCTTGCTCTTCGTTCCAAAGCATCACTCTGTCAGCTCCACTATATAGCCCGACCACTCTTTGCCGCCGCATCTTGCGCCACCGCCGTCAGCCGTAATTTCAGAGATTCTACTTCCAAAACCCTAGCGAAAAAAGCAATGGAAGACGGTAAAATCGCAAGCGTGCACTCCACAGTGTTCAAAGAATCAGAGAATTTACAAGGCAAATGCAGCGAAATCAGAGGCTACGATTTCAACGAGGGACTCGATTACTCTCGTCTCTTCGGTTCGATGATCACCACTGGTTTCCAAGCCACCAATCTCGGAGAAGCCATCGAAGTCGTCAACGAAATGCTAGATTGGAGGCTGGCAGATGAGGCTGTTGCTGAGGATTGCGGTGAGGAGGAGAGGGATGGGAAGTTTAGGGAGTCTGTGAAGTGTAAAGTGTTTTTGGGGTTCACTTCGAATTTGGTTTCGTCTGGGCTTAGAGATGTGTTTAGGTACTTGGTGGAGCGCCATGTGGTGGATGTGGTGGTCACAACTGCTGGTGGCATTGAGGAGGATCTTATCAAATGCCTTGCGCCGACTTATAAGGGTGATTTCGCTCTAGCAGGGGCGTATTTGAGGTCCAAGGGATTGAATCGTATCGGTAATTTACTGGTTCCTAATGATAACTATTGCAAATTTGAGGATTGGATCATACCGATTTTCGATCAGATGTTGAAGGAACAGATTGAACAGAATGTGCTGTGGACGCCATCTAAATTGATTGCACGCTTGGGGAAGGAAATAAATGATGAAAGATCATATCTTTACTGGGCATACAAGAACAATGTCCCAGTTTACTGTCCATCCATAACCGATGGCTCATTGGGAGACATGTTGTACTTCCATTCTTTCCGCAGCCCAGGTCTGGTTGTTGACATAGTGCAAGATATTAGGGCCATGAATGGTGAAGCTGTTCATGCAGGTCTGAGGAAGACTGGAATGATCATCCTCGGAGGAGGCCTGCCCAAACATCACATTTGCAATGCTAATATGATGCGCAATGGTGCGGATTATGCTGTGTTTATTAACACCGCACAGGAGTATGATGGTAGTGATTCTGGAGCTCGTCCTGATGAGGCTGTCTCCTGGGGGAAAATACGGGGTTCTGCCAAAACTGTTAAGGTGCACTGTGATGCAACAATTGCTTTCCCTTTTCTGGTTGCTGAGACATTTGCAAAGAAAGTTGGCAAGACTACGGCCTAG

Protein Analysis

425

Amino Acids

46.97

Weight (kDa)

6.53

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DS PF01916 102 - 413 6.3e-123 Deoxyhypusine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
Acc16I TGCGCA 1 cut(s) 1070
Acc36I ACCTGC 1 cut(s) 983
AccB7I CCANNNNNTGG 1 cut(s) 547
AciI CCGC 8 cut(s) 34, 111, 114, 129, 420, 925, 1079, 1104
AclWI GGATC 2 cut(s) 594, 728
AcsI RAATTY 3 cut(s) 238, 490, 707
AcyI GRCGYC 1 cut(s) 779
AfaI GTAC 2 cut(s) 527, 911
AfiI CCNNNNNNNGG 3 cut(s) 547, 937, 1228
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 5 cut(s) 226, 280, 664, 748, 761
AjnI CCWGG 2 cut(s) 931, 1157
AluBI AGCT 3 cut(s) 82, 983, 1136
AluI AGCT 3 cut(s) 82, 983, 1136
Alw21I GWGCWC 3 cut(s) 213, 1138, 1200
Alw26I GTCTC 4 cut(s) 308, 894, 1159, 1232
Alw44I GTGCAC 2 cut(s) 209, 1196
AlwI GGATC 2 cut(s) 594, 728
AoxI GGCC 3 cut(s) 966, 1030, 1269
ApaLI GTGCAC 2 cut(s) 209, 1196
ApeKI GCWGC 2 cut(s) 255, 927
ApoI RAATTY 3 cut(s) 238, 490, 707
Asp700I GAANNNNTTC 1 cut(s) 984
AspLEI GCGC 4 cut(s) 125, 540, 607, 1071
AspS9I GGNCC 2 cut(s) 651, 966
AsuHPI GGTGA 4 cut(s) 313, 434, 632, 989
AsuII TTCGAA 1 cut(s) 488
AvaII GGWCC 1 cut(s) 651
BaeGI GKGCMC 2 cut(s) 213, 1200
BanII GRGCYC 1 cut(s) 1138
BbsI GAAGAC 2 cut(s) 195, 1010
Bbv12I GWGCWC 3 cut(s) 213, 1138, 1200
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 267, 939
BccI CCATC 6 cut(s) 365, 431, 790, 881, 883, 1112
BceAI ACGGC 2 cut(s) 116, 123
BciT130I CCWGG 2 cut(s) 933, 1159
BclI TGATCA 2 cut(s) 318, 1014
BcoDI GTCTC 4 cut(s) 308, 894, 1159, 1232
BfaI CTAG 4 cut(s) 170, 380, 632, 1273
BfoI RGCGCY 1 cut(s) 541
BfuAI ACCTGC 1 cut(s) 983
BisI GCNGC 4 cut(s) 111, 114, 256, 928
BlsI GCNGC 4 cut(s) 112, 115, 257, 929
Bme1390I CCNGG 2 cut(s) 933, 1159
Bme18I GGWCC 1 cut(s) 651
BmgT120I GGNCC 2 cut(s) 651, 966
BmiI GGNNCC 2 cut(s) 41, 687
BmrFI CCNGG 2 cut(s) 933, 1159
BmrI ACTGGG 2 cut(s) 850, 857
BmsI GCATC 4 cut(s) 77, 125, 1056, 1195
BmuI ACTGGG 2 cut(s) 850, 857
BpiI GAAGAC 2 cut(s) 195, 1010
BplI GAGNNNNNCTC 2 cut(s) 1139, 1171
BpmI CTGGAG 1 cut(s) 1152
Bpu10I CCTNAGC 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 779
BsaJI CCNNGG 4 cut(s) 654, 931, 1021, 1158
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 937, 1228
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 5 cut(s) 331, 687, 845, 863, 1012
Bse3DI GCAATG 3 cut(s) 189, 1060, 1078
BseBI CCWGG 2 cut(s) 933, 1159
BseDI CCNNGG 4 cut(s) 654, 931, 1021, 1158
BseGI GGATG 4 cut(s) 442, 556, 875, 1017
BseLI CCNNNNNNNGG 3 cut(s) 547, 937, 1228
BseMI GCAATG 3 cut(s) 189, 1060, 1078
BseMII CTCAG 3 cut(s) 402, 989, 1227
BseNI ACTGG 5 cut(s) 331, 687, 845, 863, 1012
BseRI GAGGAG 4 cut(s) 440, 443, 596, 1041
BseSI GKGCMC 2 cut(s) 213, 1200
BseXI GCAGC 2 cut(s) 267, 939
BshFI GGCC 3 cut(s) 968, 1032, 1271
BsiHKAI GWGCWC 3 cut(s) 213, 1138, 1200
BsiSI CCGG 1 cut(s) 37
BslFI GGGAC 2 cut(s) 300, 845
BslI CCNNNNNNNGG 3 cut(s) 547, 937, 1228
BsmAI GTCTC 4 cut(s) 308, 894, 1159, 1232
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 300, 845
BsnI GGCC 3 cut(s) 968, 1032, 1271
Bsp119I TTCGAA 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 213, 1138, 1200
Bsp143I GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
BspACI CCGC 8 cut(s) 34, 111, 114, 129, 420, 925, 1079, 1104
BspANI GGCC 3 cut(s) 968, 1032, 1271
BspCNI CTCAG 3 cut(s) 403, 990, 1228
BspLI GGNNCC 2 cut(s) 41, 687
BspMI ACCTGC 1 cut(s) 983
BspPI GGATC 2 cut(s) 594, 728
BspQI GCTCTTC 1 cut(s) 60
BspT104I TTCGAA 1 cut(s) 488
BsrDI GCAATG 3 cut(s) 189, 1060, 1078
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 5 cut(s) 331, 687, 845, 863, 1012
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 4 cut(s) 654, 931, 1021, 1158
BssMI GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
BssNI GRCGYC 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 2 cut(s) 933, 1159
Bst4CI ACNGT 5 cut(s) 194, 220, 872, 1189, 1202
Bst6I CTCTTC 2 cut(s) 60, 311
BstACI GRCGYC 1 cut(s) 779
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 5 cut(s) 205, 209, 393, 799, 1034
BstDEI CTNAG 4 cut(s) 411, 509, 998, 1236
BstF5I GGATG 4 cut(s) 442, 556, 875, 1017
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 4 cut(s) 125, 540, 607, 1071
BstKTI GATC 6 cut(s) 321, 589, 723, 739, 830, 1017
BstMAI GTCTC 4 cut(s) 308, 894, 1159, 1232
BstMBI GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
BstMWI GCNNNNNNNGC 8 cut(s) 122, 179, 255, 401, 778, 989, 1085, 1214
BstNI CCWGG 2 cut(s) 933, 1159
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 2 cut(s) 931, 1157
BstSLI GKGCMC 2 cut(s) 213, 1200
BstV1I GCAGC 2 cut(s) 267, 939
BstV2I GAAGAC 2 cut(s) 195, 1010
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 3 cut(s) 968, 1032, 1271
BtsCI GGATG 4 cut(s) 442, 556, 875, 1017
BtsIMutI CAGTG 4 cut(s) 22, 225, 324, 1198
BveI ACCTGC 1 cut(s) 983
Cac8I GCNNGC 5 cut(s) 205, 209, 393, 799, 1034
CfoI GCGC 4 cut(s) 125, 540, 607, 1071
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 651, 966
CseI GACGC 1 cut(s) 787
Csp6I GTAC 2 cut(s) 526, 910
CviAII CATG 4 cut(s) 542, 904, 970, 989
CviQI GTAC 2 cut(s) 526, 910
DdeI CTNAG 4 cut(s) 411, 509, 998, 1236
DpnI GATC 6 cut(s) 320, 588, 722, 738, 829, 1016
DpnII GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
Eam1104I CTCTTC 2 cut(s) 60, 311
EarI CTCTTC 2 cut(s) 60, 311
Ecl136II GAGCTC 1 cut(s) 1136
Eco130I CCWWGG 1 cut(s) 654
Eco147I AGGCCT 1 cut(s) 1032
Eco24I GRGCYC 1 cut(s) 1138
Eco47I GGWCC 1 cut(s) 651
Eco53kI GAGCTC 1 cut(s) 1136
EcoICRI GAGCTC 1 cut(s) 1136
EcoRII CCWGG 2 cut(s) 931, 1157
EcoT14I CCWWGG 1 cut(s) 654
EcoT38I GRGCYC 1 cut(s) 1138
ErhI CCWWGG 1 cut(s) 654
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 4 cut(s) 545, 907, 973, 992
FaqI GGGAC 2 cut(s) 300, 845
FatI CATG 4 cut(s) 541, 903, 969, 988
FbaI TGATCA 2 cut(s) 318, 1014
Fnu4HI GCNGC 4 cut(s) 111, 114, 256, 928
FokI GGATG 4 cut(s) 449, 563, 862, 1004
FriOI GRGCYC 1 cut(s) 1138
Fsp4HI GCNGC 4 cut(s) 111, 114, 256, 928
FspBI CTAG 4 cut(s) 170, 380, 632, 1273
FspI TGCGCA 1 cut(s) 1070
GlaI GCGC 4 cut(s) 124, 539, 606, 1070
GluI GCNGC 4 cut(s) 111, 114, 256, 928
GsuI CTGGAG 1 cut(s) 1152
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 3 cut(s) 968, 1032, 1271
HapII CCGG 1 cut(s) 37
HgaI GACGC 1 cut(s) 787
HhaI GCGC 4 cut(s) 125, 540, 607, 1071
Hin1I GRCGYC 1 cut(s) 779
Hin1II CATG 4 cut(s) 545, 907, 973, 992
Hin6I GCGC 4 cut(s) 123, 538, 605, 1069
HinP1I GCGC 4 cut(s) 123, 538, 605, 1069
HincII GTYRAC 2 cut(s) 370, 946
HindII GTYRAC 2 cut(s) 370, 946
HinfI GANTC 6 cut(s) 152, 230, 288, 452, 664, 1127
HpaII CCGG 1 cut(s) 37
HphI GGTGA 4 cut(s) 313, 434, 632, 989
Hpy166II GTNNAC 7 cut(s) 211, 370, 483, 777, 868, 946, 1198
Hpy188I TCNGA 7 cut(s) 149, 235, 266, 350, 741, 999, 1025
Hpy188III TCNNGA 2 cut(s) 1131, 1142
Hpy8I GTNNAC 7 cut(s) 211, 370, 483, 777, 868, 946, 1198
HpyAV CCTTC 2 cut(s) 742, 802
HpyCH4III ACNGT 5 cut(s) 194, 220, 872, 1189, 1202
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 179, 255, 401, 778, 989, 1085, 1214
HpyF3I CTNAG 4 cut(s) 411, 509, 998, 1236
Hsp92I GRCGYC 1 cut(s) 779
Hsp92II CATG 4 cut(s) 545, 907, 973, 992
HspAI GCGC 4 cut(s) 123, 538, 605, 1069
Ksp22I TGATCA 2 cut(s) 318, 1014
Kzo9I GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 3 cut(s) 87, 535, 1133
Lsp1109I GCAGC 2 cut(s) 267, 939
LweI GCATC 4 cut(s) 77, 125, 1056, 1195
MaeI CTAG 4 cut(s) 170, 380, 632, 1273
MaeIII GTNAC 1 cut(s) 559
MalI GATC 6 cut(s) 320, 588, 722, 738, 829, 1016
MboI GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
MboII GAAGA 4 cut(s) 47, 200, 298, 1015
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 3 cut(s) 213, 1138, 1200
MluCI AATT 7 cut(s) 142, 238, 490, 676, 707, 788, 1212
MlyI GAGTC 2 cut(s) 282, 461
MroXI GAANNNNTTC 1 cut(s) 984
MseI TTAA 2 cut(s) 1098, 1191
MslI CAYNNNNRTG 1 cut(s) 1203
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 933, 1159
MunI CAATTG 1 cut(s) 1212
MvaI CCWGG 2 cut(s) 933, 1159
MwoI GCNNNNNNNGC 8 cut(s) 122, 179, 255, 401, 778, 989, 1085, 1214
NdeII GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
NlaIII CATG 4 cut(s) 545, 907, 973, 992
NlaIV GGNNCC 2 cut(s) 41, 687
NmuCI GTSAC 1 cut(s) 559
NsbI TGCGCA 1 cut(s) 1070
NspI RCATGY 1 cut(s) 907
NspV TTCGAA 1 cut(s) 488
PceI AGGCCT 1 cut(s) 1032
PciI ACATGT 1 cut(s) 903
PciSI GCTCTTC 1 cut(s) 60
PcsI WCGNNNNNNNCGW 2 cut(s) 279, 288
PdmI GAANNNNTTC 1 cut(s) 984
PfeI GAWTC 4 cut(s) 152, 230, 664, 1127
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 4 cut(s) 112, 115, 257, 929
PleI GAGTC 2 cut(s) 282, 460
PpsI GAGTC 2 cut(s) 282, 460
PscI ACATGT 1 cut(s) 903
PsiI TTATAA 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 1138
Psp6I CCWGG 2 cut(s) 931, 1157
PspGI CCWGG 2 cut(s) 931, 1157
PspN4I GGNNCC 2 cut(s) 41, 687
PspPI GGNCC 2 cut(s) 651, 966
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 2 cut(s) 527, 911
RsaNI GTAC 2 cut(s) 526, 910
RseI CAYNNNNRTG 1 cut(s) 1203
SacI GAGCTC 1 cut(s) 1138
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 1098, 1191
SatI GCNGC 4 cut(s) 111, 114, 256, 928
Sau3AI GATC 6 cut(s) 318, 586, 720, 736, 827, 1014
Sau96I GGNCC 2 cut(s) 651, 966
SchI GAGTC 2 cut(s) 282, 461
ScrFI CCNGG 2 cut(s) 933, 1159
SduI GDGCHC 3 cut(s) 213, 1138, 1200
SetI ASST 9 cut(s) 28, 84, 527, 653, 937, 985, 997, 1138, 1197
SfaNI GCATC 4 cut(s) 77, 125, 1056, 1195
SfuI TTCGAA 1 cut(s) 488
SinI GGWCC 1 cut(s) 651
SmiMI CAYNNNNRTG 1 cut(s) 1203
Sse9I AATT 7 cut(s) 142, 238, 490, 676, 707, 788, 1212
SseBI AGGCCT 1 cut(s) 1032
SsiI CCGC 8 cut(s) 34, 111, 114, 129, 420, 925, 1079, 1104
SspMI CTAG 4 cut(s) 170, 380, 632, 1273
SstI GAGCTC 1 cut(s) 1138
StuI AGGCCT 1 cut(s) 1032
StyD4I CCNGG 2 cut(s) 931, 1157
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 5 cut(s) 194, 220, 872, 1189, 1202
TaqI TCGA 5 cut(s) 291, 314, 360, 488, 735
TasI AATT 7 cut(s) 142, 238, 490, 676, 707, 788, 1212
TatI WGTACW 1 cut(s) 909
TauI GCSGC 2 cut(s) 113, 116
TfiI GAWTC 4 cut(s) 152, 230, 664, 1127
Tru1I TTAA 2 cut(s) 1098, 1191
Tru9I TTAA 2 cut(s) 1098, 1191
TscAI CASTG 4 cut(s) 22, 225, 331, 1205
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 255, 927
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 837, 977, 986
TspRI CASTG 4 cut(s) 22, 225, 331, 1205
Van91I CCANNNNNTGG 1 cut(s) 547
VneI GTGCAC 2 cut(s) 209, 1196
VpaK11BI GGWCC 1 cut(s) 651
XapI RAATTY 3 cut(s) 238, 490, 707
XceI RCATGY 1 cut(s) 907
XmnI GAANNNNTTC 1 cut(s) 984
XspI CTAG 4 cut(s) 170, 380, 632, 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.