RLG00000016206

Deoxyhypusine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002811
Physical Location & Seq
Forward (+)
4745762 .. 4747510
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016206

Sequence Viewer

Length: 1143 bp
ATGGAAGACGGTAAAATCGCAAGCGTGCACTCCACAGTGTTCAAAGAATCAGAGAATTTACAAGGCAAATGCAGCGAAATCAGAGGCTACGATTTCAACGAAGGACTCGATTACTCGCGTCTCTTCGGTTCGATGATCACTACTGGTTTCCGAGCCACAAATCTCGGAGAAGCCATCGAAGTCGTCAACCAAATGTGGGACTGTGCAGCTAGATTGGAGGCTGCAGCTGAATATTGCGGTGAGGAGGAGAGGGATGGGAAGTTCAGGGAGTCTGTGAAGTGTAAAGTGTTTTTGGGTTTCACTTCGAATTTGGTTTCGTCTGGGCTTAGAGATGTGTTTAGGTACTTGGTGGAGCACCATGTGGTGGATGTGGTGGTTACAACTGCTGGTGGCATTGAGGAGGATCTTATCAAATGCCTTGCGCCGACTTATAAGGGTGATTTCACACCAGCAGGGGCGTATTTGAGGTCGAAGGGATTGAATGGTATCGGTAATTTGCTGGTTCCTAATGATAACTATTGCAAGTTTGAGGATTGGATCATACAGATTTTCGATCAGATGTTGAAGGAACAGATTGAACAGAATGTACTGTGGACGCCATCGAAATTGATTGCACGCTTGGGGAAGGAAATAAATGATGACAGATCGTACCTTGACTGGGCATACAAGTTAATAGGGGTTGTCTGTGCAGTAAGAAGTGATTATTTTTCTTTTGGATGTGTTCCAGAACAATGTCCCAGTTTACTGTCCATCCATTCTTTGGGAGACATGTTGAACTTCCATTCTTTTTGCAGCCCAGGTCTGGTTGTTGACATAGTGCAAGATATTAGGGCCATGAATGGTGAAGCTGTTCATTCAGGTCTGAGGAAGACTGGAGTGATCATCCTTGGAGGAGGCCTGCCCAAGCATCACATTTGCAATGCTAATATGATGCGCAATGGTGCGGATTATGCTGTGTTTATTAACACTGCACAGGAGTATGATGGTAGTGATTCTGGAGCTCGTCCTGATGAGGCTGTCTCCTGGGGGACAATACGGGGTTCTGCCAAAACTGTTAGGTGTGTGCACTGTGATGCAACAATCGCTTTCCCTTTTCTGGTTGCTGAGACATTTGCAAAGAAAGTTAGCAAGACTACGACCTAG

Protein Analysis

381

Amino Acids

41.94

Weight (kDa)

5.37

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DS PF01916 41 - 369 7e-98 Deoxyhypusine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
Acc16I TGCGCA 1 cut(s) 935
AccB7I CCANNNNNTGG 2 cut(s) 364, 760
AccII CGCG 1 cut(s) 118
AciI CCGC 2 cut(s) 237, 944
AclWI GGATC 2 cut(s) 411, 545
AcsI RAATTY 2 cut(s) 55, 307
AcyI GRCGYC 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 361
AfaI GTAC 3 cut(s) 344, 588, 650
AfiI CCNNNNNNNGG 6 cut(s) 196, 364, 658, 760, 802, 1096
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 6 cut(s) 43, 97, 481, 565, 578, 775
AjnI CCWGG 2 cut(s) 796, 1022
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 4 cut(s) 209, 227, 848, 1001
AluI AGCT 4 cut(s) 209, 227, 848, 1001
Alw21I GWGCWC 4 cut(s) 30, 357, 1003, 1068
Alw26I GTCTC 4 cut(s) 125, 759, 1024, 1100
Alw44I GTGCAC 2 cut(s) 26, 1064
AlwI GGATC 2 cut(s) 411, 545
AoxI GGCC 2 cut(s) 831, 895
ApaLI GTGCAC 2 cut(s) 26, 1064
ApeKI GCWGC 5 cut(s) 72, 206, 221, 224, 792
ApoI RAATTY 2 cut(s) 55, 307
Asp700I GAANNNNTTC 1 cut(s) 849
AspLEI GCGC 2 cut(s) 424, 936
AspS9I GGNCC 1 cut(s) 831
AsuHPI GGTGA 3 cut(s) 251, 449, 854
AsuII TTCGAA 1 cut(s) 305
BaeGI GKGCMC 2 cut(s) 30, 1068
BanII GRGCYC 1 cut(s) 1003
BbsI GAAGAC 2 cut(s) 12, 875
Bbv12I GWGCWC 4 cut(s) 30, 357, 1003, 1068
BbvI GCAGC 5 cut(s) 84, 208, 218, 236, 804
BccI CCATC 5 cut(s) 182, 248, 607, 758, 977
BciT130I CCWGG 2 cut(s) 798, 1024
BclI TGATCA 2 cut(s) 135, 879
BcoDI GTCTC 4 cut(s) 125, 759, 1024, 1100
BfaI CTAG 2 cut(s) 210, 1141
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 5 cut(s) 73, 207, 222, 225, 793
BlsI GCNGC 5 cut(s) 74, 208, 223, 226, 794
Bme1390I CCNGG 2 cut(s) 798, 1024
BmgT120I GGNCC 1 cut(s) 831
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 2 cut(s) 798, 1024
BmrI ACTGGG 2 cut(s) 667, 732
BmsI GCATC 3 cut(s) 916, 921, 1063
BmuI ACTGGG 2 cut(s) 667, 732
BpiI GAAGAC 2 cut(s) 12, 875
BplI GAGNNNNNCTC 2 cut(s) 1004, 1036
BpmI CTGGAG 2 cut(s) 894, 1017
Bpu14I TTCGAA 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 596
BsaJI CCNNGG 3 cut(s) 796, 886, 1023
Bsc4I CCNNNNNNNGG 6 cut(s) 196, 364, 658, 760, 802, 1096
Bse1I ACTGG 4 cut(s) 148, 662, 738, 877
Bse3DI GCAATG 2 cut(s) 925, 943
BseBI CCWGG 2 cut(s) 798, 1024
BseDI CCNNGG 3 cut(s) 796, 886, 1023
BseGI GGATG 5 cut(s) 259, 373, 722, 750, 882
BseLI CCNNNNNNNGG 6 cut(s) 196, 364, 658, 760, 802, 1096
BseMI GCAATG 2 cut(s) 925, 943
BseMII CTCAG 2 cut(s) 854, 1095
BseNI ACTGG 4 cut(s) 148, 662, 738, 877
BseRI GAGGAG 4 cut(s) 257, 260, 413, 906
BseSI GKGCMC 2 cut(s) 30, 1068
BseXI GCAGC 5 cut(s) 84, 208, 218, 236, 804
BsgI GTGCAG 3 cut(s) 225, 708, 954
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 833, 897
BsiHKAI GWGCWC 4 cut(s) 30, 357, 1003, 1068
BslFI GGGAC 3 cut(s) 212, 720, 1042
BslI CCNNNNNNNGG 6 cut(s) 196, 364, 658, 760, 802, 1096
BsmAI GTCTC 4 cut(s) 125, 759, 1024, 1100
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 3 cut(s) 212, 720, 1042
BsnI GGCC 2 cut(s) 833, 897
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 4 cut(s) 30, 357, 1003, 1068
Bsp143I GATC 6 cut(s) 135, 403, 537, 553, 644, 879
BspACI CCGC 2 cut(s) 237, 944
BspANI GGCC 2 cut(s) 833, 897
BspCNI CTCAG 2 cut(s) 855, 1096
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 504
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 2 cut(s) 411, 545
BspT104I TTCGAA 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 925, 943
BsrI ACTGG 4 cut(s) 148, 662, 738, 877
BssECI CCNNGG 3 cut(s) 796, 886, 1023
BssMI GATC 6 cut(s) 135, 403, 537, 553, 644, 879
BssNI GRCGYC 1 cut(s) 596
BssT1I CCWWGG 1 cut(s) 886
Bst2UI CCWGG 2 cut(s) 798, 1024
Bst4CI ACNGT 7 cut(s) 11, 37, 203, 591, 747, 1054, 1070
Bst6I CTCTTC 1 cut(s) 128
BstACI GRCGYC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 22, 26, 616, 899
BstDEI CTNAG 3 cut(s) 326, 863, 1104
BstF5I GGATG 5 cut(s) 259, 373, 722, 750, 882
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 2 cut(s) 424, 936
BstKTI GATC 6 cut(s) 138, 406, 540, 556, 647, 882
BstMAI GTCTC 4 cut(s) 125, 759, 1024, 1100
BstMBI GATC 6 cut(s) 135, 403, 537, 553, 644, 879
BstMWI GCNNNNNNNGC 3 cut(s) 72, 950, 1082
BstNI CCWGG 2 cut(s) 798, 1024
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 2 cut(s) 796, 1022
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 2 cut(s) 30, 1068
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 5 cut(s) 84, 208, 218, 236, 804
BstV2I GAAGAC 2 cut(s) 12, 875
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 2 cut(s) 833, 897
BtsCI GGATG 5 cut(s) 259, 373, 722, 750, 882
BtsI GCAGTG 1 cut(s) 966
BtsIMutI CAGTG 3 cut(s) 42, 966, 1066
Cac8I GCNNGC 4 cut(s) 22, 26, 616, 899
CfoI GCGC 2 cut(s) 424, 936
Cfr13I GGNCC 1 cut(s) 831
CseI GACGC 2 cut(s) 107, 604
Csp6I GTAC 3 cut(s) 343, 587, 649
CviAII CATG 3 cut(s) 359, 769, 835
CviQI GTAC 3 cut(s) 343, 587, 649
DdeI CTNAG 3 cut(s) 326, 863, 1104
DpnI GATC 6 cut(s) 137, 405, 539, 555, 646, 881
DpnII GATC 6 cut(s) 135, 403, 537, 553, 644, 879
DraIII CACNNNGTG 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Ecl136II GAGCTC 1 cut(s) 1001
Eco130I CCWWGG 1 cut(s) 886
Eco147I AGGCCT 1 cut(s) 897
Eco24I GRGCYC 1 cut(s) 1003
Eco53kI GAGCTC 1 cut(s) 1001
EcoICRI GAGCTC 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 796, 1022
EcoT14I CCWWGG 1 cut(s) 886
EcoT38I GRGCYC 1 cut(s) 1003
ErhI CCWWGG 1 cut(s) 886
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 3 cut(s) 362, 772, 838
FaqI GGGAC 3 cut(s) 212, 720, 1042
FatI CATG 3 cut(s) 358, 768, 834
FbaI TGATCA 2 cut(s) 135, 879
Fnu4HI GCNGC 5 cut(s) 73, 207, 222, 225, 793
FokI GGATG 5 cut(s) 266, 380, 729, 737, 869
FriOI GRGCYC 1 cut(s) 1003
Fsp4HI GCNGC 5 cut(s) 73, 207, 222, 225, 793
FspBI CTAG 2 cut(s) 210, 1141
FspI TGCGCA 1 cut(s) 935
GlaI GCGC 2 cut(s) 423, 935
GluI GCNGC 5 cut(s) 73, 207, 222, 225, 793
GsuI CTGGAG 2 cut(s) 894, 1017
HaeIII GGCC 2 cut(s) 833, 897
HgaI GACGC 2 cut(s) 107, 604
HhaI GCGC 2 cut(s) 424, 936
Hin1I GRCGYC 1 cut(s) 596
Hin1II CATG 3 cut(s) 362, 772, 838
Hin6I GCGC 2 cut(s) 422, 934
HinP1I GCGC 2 cut(s) 422, 934
HincII GTYRAC 2 cut(s) 187, 811
HindII GTYRAC 2 cut(s) 187, 811
HinfI GANTC 4 cut(s) 47, 105, 269, 992
HphI GGTGA 3 cut(s) 251, 449, 854
Hpy166II GTNNAC 6 cut(s) 28, 187, 594, 743, 811, 1066
Hpy188I TCNGA 6 cut(s) 52, 83, 152, 167, 558, 864
Hpy188III TCNNGA 3 cut(s) 725, 996, 1007
Hpy8I GTNNAC 6 cut(s) 28, 187, 594, 743, 811, 1066
HpyAV CCTTC 4 cut(s) 95, 466, 559, 619
HpyCH4III ACNGT 7 cut(s) 11, 37, 203, 591, 747, 1054, 1070
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 950, 1082
HpyF3I CTNAG 3 cut(s) 326, 863, 1104
Hsp92I GRCGYC 1 cut(s) 596
Hsp92II CATG 3 cut(s) 362, 772, 838
HspAI GCGC 2 cut(s) 422, 934
Ksp22I TGATCA 2 cut(s) 135, 879
Kzo9I GATC 6 cut(s) 135, 403, 537, 553, 644, 879
LmnI GCTCC 2 cut(s) 352, 998
Lsp1109I GCAGC 5 cut(s) 84, 208, 218, 236, 804
LweI GCATC 3 cut(s) 916, 921, 1063
MaeI CTAG 2 cut(s) 210, 1141
MaeIII GTNAC 1 cut(s) 376
MalI GATC 6 cut(s) 137, 405, 539, 555, 646, 881
MboI GATC 6 cut(s) 135, 403, 537, 553, 644, 879
MboII GAAGA 3 cut(s) 17, 115, 880
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 4 cut(s) 30, 357, 1003, 1068
MluCI AATT 4 cut(s) 55, 307, 493, 605
MlyI GAGTC 2 cut(s) 99, 278
MroXI GAANNNNTTC 1 cut(s) 849
MseI TTAA 2 cut(s) 671, 963
MslI CAYNNNNRTG 1 cut(s) 1071
MspA1I CMGCKG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 798, 1024
MvaI CCWGG 2 cut(s) 798, 1024
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 3 cut(s) 72, 950, 1082
NdeII GATC 6 cut(s) 135, 403, 537, 553, 644, 879
NlaIII CATG 3 cut(s) 362, 772, 838
NlaIV GGNNCC 1 cut(s) 504
NsbI TGCGCA 1 cut(s) 935
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 1 cut(s) 305
PceI AGGCCT 1 cut(s) 897
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 105
PdmI GAANNNNTTC 1 cut(s) 849
PfeI GAWTC 2 cut(s) 47, 992
PflMI CCANNNNNTGG 2 cut(s) 364, 760
PkrI GCNGC 5 cut(s) 74, 208, 223, 226, 794
PleI GAGTC 2 cut(s) 99, 277
PpsI GAGTC 2 cut(s) 99, 277
PscI ACATGT 1 cut(s) 768
PsiI TTATAA 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 1003
Psp6I CCWGG 2 cut(s) 796, 1022
PspGI CCWGG 2 cut(s) 796, 1022
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 1 cut(s) 831
PsrI GAACNNNNNNTAC 2 cut(s) 570, 602
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 3 cut(s) 344, 588, 650
RsaNI GTAC 3 cut(s) 343, 587, 649
RseI CAYNNNNRTG 1 cut(s) 1071
SacI GAGCTC 1 cut(s) 1003
SaqAI TTAA 2 cut(s) 671, 963
SatI GCNGC 5 cut(s) 73, 207, 222, 225, 793
Sau3AI GATC 6 cut(s) 135, 403, 537, 553, 644, 879
Sau96I GGNCC 1 cut(s) 831
SchI GAGTC 2 cut(s) 99, 278
ScrFI CCNGG 2 cut(s) 798, 1024
SduI GDGCHC 4 cut(s) 30, 357, 1003, 1068
SfaNI GCATC 3 cut(s) 916, 921, 1063
SfcI CTRYAG 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 1071
Sse9I AATT 4 cut(s) 55, 307, 493, 605
SseBI AGGCCT 1 cut(s) 897
SsiI CCGC 2 cut(s) 237, 944
SspI AATATT 1 cut(s) 233
SspMI CTAG 2 cut(s) 210, 1141
SstI GAGCTC 1 cut(s) 1003
StuI AGGCCT 1 cut(s) 897
StyD4I CCNGG 2 cut(s) 796, 1022
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 7 cut(s) 11, 37, 203, 591, 747, 1054, 1070
TaqI TCGA 7 cut(s) 108, 131, 177, 305, 470, 552, 602
TasI AATT 4 cut(s) 55, 307, 493, 605
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 2 cut(s) 47, 992
Tru1I TTAA 2 cut(s) 671, 963
Tru9I TTAA 2 cut(s) 671, 963
TscAI CASTG 3 cut(s) 42, 973, 1073
TseI GCWGC 5 cut(s) 72, 206, 221, 224, 792
TspDTI ATGAA 2 cut(s) 842, 851
TspRI CASTG 3 cut(s) 42, 973, 1073
Van91I CCANNNNNTGG 2 cut(s) 364, 760
VneI GTGCAC 2 cut(s) 26, 1064
XapI RAATTY 2 cut(s) 55, 307
XceI RCATGY 1 cut(s) 772
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
XmnI GAANNNNTTC 1 cut(s) 849
XspI CTAG 2 cut(s) 210, 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.