FvH4_1g10590

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004833
Physical Location & Seq
Reverse (-)
5810989 .. 5811620
632 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10590.t1

Sequence Viewer

Length: 483 bp
ATGACTGATGGTTTTATGCAGTGCTCAGCAAACTGCATACCCTTGTCACCGATAAGCTTCTTGGAGCGAGCAGCTGTCGTCAATGGCGATAAGCCTTCCGTCGTCTACGGCGGCGTAAGGTATTCGTGGAAGGAGACGCATCAAAGATGCCTCAACTTCGCTTCAGCTTTGTTCCGGTTAGGAGTATCCCGCCATGATGTTGTAAGTGCGGACGTCCGCACGGCTCTTAAAGTGCGGACGTCCCTCCTTTCGCCATCGTACGACGCCGACGAGGGCGCGCCTCCACTCACGGACTCCAGCAGCTTCTCCGACCACTTTTCTGATATTGGGCTCTACTTAACCTGTTTTGGGTTACAGCCATATCCATCTGTCCTCGAGAGAGTCCAATGGTTTGGGCCAATGACTAACAGTAGAATAGAAACTGATGAAGGGATCAAATCATCTCAGAGCTTCAGAACAATCTTCTGGGACAGAAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

161

Amino Acids

17.88

Weight (kDa)

5.89

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 69 4.1e-07 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 216, 242
AccI GTMKAC 1 cut(s) 105
AccII CGCG 1 cut(s) 278
AciI CCGC 5 cut(s) 111, 190, 209, 217, 235
AclWI GGATC 1 cut(s) 440
AcuI CTGAAG 2 cut(s) 147, 436
AcyI GRCGYC 3 cut(s) 213, 239, 264
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 348
AluBI AGCT 5 cut(s) 57, 74, 167, 303, 450
AluI AGCT 5 cut(s) 57, 74, 167, 303, 450
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 2 cut(s) 128, 471
AlwI GGATC 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 2 cut(s) 71, 300
AscI GGCGCGCC 1 cut(s) 276
AspLEI GCGC 2 cut(s) 278, 280
AspS9I GGNCC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 374
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 83, 312
BccI CCATC 3 cut(s) 2, 262, 373
BceAI ACGGC 2 cut(s) 124, 237
BciVI GTATCC 1 cut(s) 196
BcoDI GTCTC 2 cut(s) 128, 471
BfuI GTATCC 1 cut(s) 196
BisI GCNGC 3 cut(s) 72, 112, 301
BlpI GCTNAGC 1 cut(s) 25
BlsI GCNGC 3 cut(s) 73, 113, 302
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 395
BmsI GCATC 2 cut(s) 137, 148
BpmI CTGGAG 1 cut(s) 280
Bpu1102I GCTNAGC 1 cut(s) 25
BsaHI GRCGYC 3 cut(s) 213, 239, 264
BsaWI WCCGGW 1 cut(s) 174
Bsc4I CCNNNNNNNGG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 39, 458
BsePI GCGCGC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 83, 312
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 175
BsiWI CGTACG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 348
BsmAI GTCTC 2 cut(s) 128, 471
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 26, 333
Bsp143I GATC 1 cut(s) 432
Bsp1720I GCTNAGC 1 cut(s) 25
BspACI CCGC 5 cut(s) 111, 190, 209, 217, 235
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 2 cut(s) 38, 457
BspFNI CGCG 1 cut(s) 278
BspPI GGATC 1 cut(s) 440
BssHII GCGCGC 1 cut(s) 276
BssMI GATC 1 cut(s) 432
BssNI GRCGYC 3 cut(s) 213, 239, 264
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 469
BstACI GRCGYC 3 cut(s) 213, 239, 264
BstC8I GCNNGC 2 cut(s) 69, 278
BstDEI CTNAG 2 cut(s) 25, 444
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 2 cut(s) 278, 280
BstKTI GATC 1 cut(s) 435
BstMAI GTCTC 2 cut(s) 128, 471
BstMBI GATC 1 cut(s) 432
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 83, 312
BstXI CCANNNNNNTGG 1 cut(s) 392
BsuI GTATCC 1 cut(s) 196
BsuRI GGCC 1 cut(s) 397
BtsI GCAGTG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 69, 278
CfoI GCGC 2 cut(s) 278, 280
Cfr13I GGNCC 1 cut(s) 395
CseI GACGC 2 cut(s) 145, 272
Csp6I GTAC 1 cut(s) 259
CviAII CATG 1 cut(s) 194
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 2 cut(s) 25, 444
DpnI GATC 1 cut(s) 434
DpnII GATC 1 cut(s) 432
Eam1104I CTCTTC 1 cut(s) 469
EarI CTCTTC 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 333
Eco57I CTGAAG 2 cut(s) 147, 436
Eco88I CYCGRG 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 1 cut(s) 197
FaiI YATR 4 cut(s) 17, 38, 195, 361
FaqI GGGAC 1 cut(s) 226
FatI CATG 1 cut(s) 193
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 72, 112, 301
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 3 cut(s) 72, 112, 301
GlaI GCGC 2 cut(s) 277, 279
GluI GCNGC 3 cut(s) 72, 112, 301
GsuI CTGGAG 1 cut(s) 280
HaeIII GGCC 1 cut(s) 397
HapII CCGG 1 cut(s) 175
HgaI GACGC 2 cut(s) 145, 272
HhaI GCGC 2 cut(s) 278, 280
Hin1I GRCGYC 3 cut(s) 213, 239, 264
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 2 cut(s) 276, 278
HinP1I GCGC 2 cut(s) 276, 278
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 2 cut(s) 293, 381
HpaII CCGG 1 cut(s) 175
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 310, 322, 447, 455
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 3 cut(s) 104, 266, 272
HpyAV CCTTC 3 cut(s) 105, 124, 422
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 2 cut(s) 213, 239
HpyCH4V TGCA 2 cut(s) 19, 36
HpyF3I CTNAG 2 cut(s) 25, 444
HpySE526I ACGT 2 cut(s) 213, 239
Hsp92I GRCGYC 3 cut(s) 213, 239, 264
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 2 cut(s) 276, 278
Kzo9I GATC 1 cut(s) 432
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 4 cut(s) 188, 310, 355, 451
Lsp1109I GCAGC 2 cut(s) 83, 312
LweI GCATC 2 cut(s) 137, 148
MaeII ACGT 2 cut(s) 213, 239
MaeIII GTNAC 2 cut(s) 45, 351
MalI GATC 1 cut(s) 434
MboI GATC 1 cut(s) 432
MboII GAAGA 1 cut(s) 454
MhlI GDGCHC 2 cut(s) 26, 333
MlyI GAGTC 2 cut(s) 287, 390
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 5 cut(s) 161, 254, 265, 291, 383
MseI TTAA 2 cut(s) 228, 338
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 278
NdeII GATC 1 cut(s) 432
NlaIII CATG 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 45
PaeR7I CTCGAG 1 cut(s) 374
PalAI GGCGCGCC 1 cut(s) 276
PauI GCGCGC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 267
Pfl23II CGTACG 1 cut(s) 258
PkrI GCNGC 3 cut(s) 73, 113, 302
PleI GAGTC 2 cut(s) 287, 389
PpsI GAGTC 2 cut(s) 287, 389
PspLI CGTACG 1 cut(s) 258
PspPI GGNCC 1 cut(s) 395
PteI GCGCGC 1 cut(s) 276
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 2 cut(s) 228, 338
SatI GCNGC 3 cut(s) 72, 112, 301
Sau3AI GATC 1 cut(s) 432
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 2 cut(s) 287, 390
SduI GDGCHC 2 cut(s) 26, 333
SetI ASST 9 cut(s) 59, 76, 122, 169, 216, 242, 305, 344, 452
SfaNI GCATC 2 cut(s) 137, 148
Sfr274I CTCGAG 1 cut(s) 374
SgsI GGCGCGCC 1 cut(s) 276
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SsiI CCGC 5 cut(s) 111, 190, 209, 217, 235
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 2 cut(s) 216, 242
TaqI TCGA 1 cut(s) 375
TauI GCSGC 1 cut(s) 114
Tru1I TTAA 2 cut(s) 228, 338
Tru9I TTAA 2 cut(s) 228, 338
TscAI CASTG 1 cut(s) 26
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 2 cut(s) 71, 300
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 1 cut(s) 441
TspGWI ACGGA 2 cut(s) 88, 305
TspRI CASTG 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 105
ZraI GACGTC 2 cut(s) 214, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.