Rh6DG148000

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004833
Physical Location & Seq
Forward (+)
21475186 .. 21475635
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG148000.1

Sequence Viewer

Length: 450 bp
ATGGCTATGAACCAGTTGCTGCTCAGGAACTCATGGACTCGCCGCCTGGTTTCCACTCTCAGTCCTCCTCTCTCTCACTTGATCACTCTCGCTCATTCTTACCGTGGATACTGCTCTGACTCTTCTTCTGGGATTAATAGTAACAACTCTGAGCTTGAAGAGTTATGGAGTTCAAAGCAGGGTTTAGTTCGGTGCTCGGCAAATCACGCCCCTTTATCTCCAATAGGATTCTTGGAGCGAGCTGCAAAGGCCTACAGGGACAGGACCTCTGTAATCTATGGCTCTCTGAAATACACTTGGGCGCAAACCCATCAACGCTGCCTTAAATTAGCTTCCGCTCTCACCCAGTTGGGGATTTCTCGCGGTGATGTGGTGAGTGATTACTTCTTCATCTCCATTACTTGTGTCATGATGTTATGTTTTCAAGATAGATCTTCAAAACCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

149

Amino Acids

16.7

Weight (kDa)

9.43

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 78 - 133 5.6e-09 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 338
AccII CGCG 1 cut(s) 363
AciI CCGC 3 cut(s) 43, 336, 363
AfiI CCNNNNNNNGG 1 cut(s) 351
AgsI TTSAA 4 cut(s) 158, 174, 425, 438
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 3 cut(s) 154, 242, 332
AluI AGCT 3 cut(s) 154, 242, 332
Alw21I GWGCWC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 19
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 3 cut(s) 19, 242, 318
AseI ATTAAT 1 cut(s) 135
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 264
AsuHPI GGTGA 3 cut(s) 334, 377, 385
AvaII GGWCC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 3 cut(s) 6, 229, 305
BccI CCATC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 47
BciVI GTATCC 1 cut(s) 101
BclI TGATCA 1 cut(s) 81
BfmI CTRYAG 1 cut(s) 253
BfuI GTATCC 1 cut(s) 101
BglII AGATCT 1 cut(s) 431
BisI GCNGC 4 cut(s) 20, 43, 243, 319
BlsI GCNGC 4 cut(s) 21, 44, 244, 320
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 1 cut(s) 340
BmuI ACTGGG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 103
Bsc4I CCNNNNNNNGG 1 cut(s) 351
Bse1I ACTGG 2 cut(s) 13, 346
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 103
BseLI CCNNNNNNNGG 1 cut(s) 351
BseMII CTCAG 3 cut(s) 37, 73, 141
BseNI ACTGG 2 cut(s) 13, 346
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 6, 229, 305
Bsh1236I CGCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 197
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 2 cut(s) 81, 431
BspACI CCGC 3 cut(s) 43, 336, 363
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 3 cut(s) 36, 72, 142
BspFNI CGCG 1 cut(s) 363
BspHI TCATGA 1 cut(s) 408
BsrBI CCGCTC 1 cut(s) 338
BsrI ACTGG 2 cut(s) 13, 346
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 2 cut(s) 81, 431
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 2 cut(s) 127, 153
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 3 cut(s) 23, 59, 150
BstDSI CCRYGG 1 cut(s) 103
BstFNI CGCG 1 cut(s) 363
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 2 cut(s) 84, 434
BstMBI GATC 2 cut(s) 81, 431
BstMWI GCNNNNNNNGC 2 cut(s) 206, 248
BstNI CCWGG 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 253
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 3 cut(s) 6, 229, 305
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BsuI GTATCC 1 cut(s) 101
BsuRI GGCC 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 240
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 408
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 264
CviAII CATG 2 cut(s) 33, 409
CviJI RGCY 6 cut(s) 5, 154, 242, 251, 282, 332
CviKI_1 RGCY 6 cut(s) 5, 154, 242, 251, 282, 332
DdeI CTNAG 3 cut(s) 23, 59, 150
DpnI GATC 2 cut(s) 83, 433
DpnII GATC 2 cut(s) 81, 431
Eam1104I CTCTTC 2 cut(s) 127, 153
EarI CTCTTC 2 cut(s) 127, 153
Eco147I AGGCCT 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 36, 412
FaiI YATR 6 cut(s) 8, 34, 166, 279, 410, 418
FaqI GGGAC 1 cut(s) 272
FatI CATG 2 cut(s) 32, 408
FbaI TGATCA 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 20, 43, 243, 319
Fsp4HI GCNGC 4 cut(s) 20, 43, 243, 319
GlaI GCGC 1 cut(s) 303
GluI GCNGC 4 cut(s) 20, 43, 243, 319
HaeIII GGCC 1 cut(s) 251
HhaI GCGC 1 cut(s) 304
Hin1II CATG 2 cut(s) 36, 412
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HinfI GANTC 3 cut(s) 37, 119, 228
HphI GGTGA 3 cut(s) 334, 377, 385
Hpy188I TCNGA 3 cut(s) 118, 151, 288
Hpy188III TCNNGA 3 cut(s) 25, 409, 425
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 248
HpyF3I CTNAG 3 cut(s) 23, 59, 150
Hsp92II CATG 2 cut(s) 36, 412
HspAI GCGC 1 cut(s) 302
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 2 cut(s) 81, 431
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 9 cut(s) 10, 26, 32, 59, 114, 164, 241, 247, 359
Lsp1109I GCAGC 3 cut(s) 6, 229, 305
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 83, 433
MbiI CCGCTC 1 cut(s) 338
MboI GATC 2 cut(s) 81, 431
MboII GAAGA 5 cut(s) 114, 117, 170, 379, 426
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 2 cut(s) 326, 445
MlyI GAGTC 2 cut(s) 31, 113
MnlI CCTC 3 cut(s) 75, 78, 277
MseI TTAA 2 cut(s) 135, 324
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 206, 248
NdeII GATC 2 cut(s) 81, 431
NlaIII CATG 2 cut(s) 36, 412
NmeAIII GCCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 408
PceI AGGCCT 1 cut(s) 251
PfeI GAWTC 1 cut(s) 228
PkrI GCNGC 4 cut(s) 21, 44, 244, 320
PleI GAGTC 2 cut(s) 31, 113
PpsI GAGTC 2 cut(s) 31, 113
PpuMI RGGWCCY 1 cut(s) 264
PshBI ATTAAT 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspPI GGNCC 1 cut(s) 264
PspPPI RGGWCCY 1 cut(s) 264
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 431
SaqAI TTAA 2 cut(s) 135, 324
SatI GCNGC 4 cut(s) 20, 43, 243, 319
Sau3AI GATC 2 cut(s) 81, 431
Sau96I GGNCC 1 cut(s) 264
SchI GAGTC 2 cut(s) 31, 113
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 197
SetI ASST 4 cut(s) 156, 244, 269, 334
SfcI CTRYAG 1 cut(s) 253
SinI GGWCC 1 cut(s) 264
Sse9I AATT 2 cut(s) 326, 445
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 3 cut(s) 43, 336, 363
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 104
TasI AATT 2 cut(s) 326, 445
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 2 cut(s) 135, 324
Tru9I TTAA 2 cut(s) 135, 324
TseI GCWGC 3 cut(s) 19, 242, 318
TspDTI ATGAA 2 cut(s) 23, 379
VpaK11BI GGWCC 1 cut(s) 264
VspI ATTAAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.