Rorug02G0253400

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004833
Physical Location & Seq
Reverse (-)
26557697 .. 26562230
4534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0253400.1

Sequence Viewer

Length: 3189 bp
ATGGTGCGTTTTCCAAAACTCTCAACCGCTGCTGCATGGAATTCCATTATACGAGAATCTGTAAACCAAAGCCATCCCCAAAAAGCCCTCCTTCTCTTTTGTCAGATGAAGCAAAATGGTTTGGAACCTAACAACTTTACCTTTCCCTTCTTGGCTAAAGCATGTGCCAAGCTCTCCAACCTCAAATTATCCCAGACCATTCACACCCATGTTGTAAAATCACCATTTCAGTCTGATATCTTTGTGCAAACAGCTATTCTAGATATGTATGTCAAGTGTAATCAATTGTGTGATGCTTACAACCTGTTCGAGAAAATTACCATGAAAGACACGGCTTCCTGCAATGTGATGCTTATGGGTTTTGTGCAGTCGGGTTTTCTTGATAAAGTGTTATGTTTGGTCCATGACATGAGGTTTGCCGGGATTCAGCCTGATACAATTACGGTTATGGGGTTGATTCAGGCGAGTTTGGCTACAAAGAATTTGGAATTGGTGAAATCAATTCATGCGTTTGGAATTCAAATTGGGATAGAATGCGATGTTTCAATGGCTAATACTTGGATCTCTGCCTATTCCAAATGTGCTGATTTGGAGTCAGCCAAGGCTATATTTGATAGGATTGACATGGGTGTGAGGACTGTTGTCTCATGGAATTCGATGATAGCGGGATATTCAAACTCAAATAAATTTCTTGATGTTCTAAATGTTTACAAACAGATGTTGTCTGATGGATACAGACCTGATATAAGCACTATTTTAAGCGTCCTTTCTTCCTGCAATCAATCCAATAAATTGGTCCAAGGTATGCTGATTCATTGTCAAGGAATTCAACTGGGCTGTGATTCTGATATTTTTGTTATCAATGACCTTATATCTATGTATTCCAAGTGTGGTGATATACTTTCTGCTAGGTTTTTATTCGATGGCATGCATGATAGAACTTGTGTCTCATGGACTGCTATGATCAGCGGGTATGCTGAAAATGGGAACCTGAATGAGGCGCTTGAGTTGTTTCATTTTATGGAAGCAGCTGGTGAGAAACCTGATTTGGTTACTGTTCTTTCTCTCATTTCAGGCTGTGGTCATACAGGAGCACTTGATCATGGAAAATGGATCCATAACTATGCCTTTTCAAATGGTTTAAGAGACAATACAGTAGTTTGCAATGCACTAATTGACATGTATGCAAAATGTGGAAATATAAACAATGCTCGAGAGCTCTTCTACACCTTGCCAGTGACAACTGTTGTTTCCTGGACATCTATGATCGCGGGGTATGCTCTGAATGGTCAATACAAGGAAGCTCTGGACCTTTTCTATTTGATGCTGGAGACAGGCATGAAACCAAACCGCTTAACATTTCTTGCTATTCTTCAAGCTTGTACTCATGCAGGTCTGCTTGAGAAAGGAATGGAGTTCTTTAACAAGATGACAGAAGTTTATTATATGAATCCTGGGGTAGATCATTATTCGTGTATGGTCAATCTTCTTGGACGTAAAGGAAAATTAGAAGAAGCAGTGGAGCTTGTTAAAAGTATGCCAATGAAACCTGATGCTGGCATATGGGGTGCGTTACTTAGTGCTTGCAGGATTCACCATAACATGGAGATTGGTGAATATGCATTTAGCCGTCTCCTTGAGTTAGAGCCCCGGGTTGCAGTTCCATATGTGGAGATGGCCAACATATATGCATCACAGAGAAGGTGGGATGAAGTTGCTGCATTAAGAACAGTGATGAAATTTAACAAAGTGAAAAAGCTTCCTGGGCAGAGCCTTGTTCATGTTAATGGGAAGCCTCATGTATTTACAGTTGAAGATAGTGGTCATCCTGAAGCCTTATTTATATATGCAATGATAGGAATTGCTCTGGAAGCCAACACCATGCTGAGCTACCAAGAAGATTGTGGAGATCTCAATATCACACTTCCATACCAAAATGATTTTGAGGTAGCAAAACTTTCTTCCACCCATCTAAGTGATTTAACAATCTTGGACACTCCAAACAAGGTTACAGTCATAGGTTGTGAATACTCCTTTGATTTTGTGAGAGGGACAAACAAGGCACCACCCAACAACAACACCAACAATAGTCTGACTCATGCATCTTGTTCTAGTAGTATTGGCTGTTGCAGTTCTTCCATTCAGACCATTCCCAGAGGCTTCAGGTCTTTCTTTACTATGGAAGAAATGAGCATTACAGGAAACACCAAACCATTTAGCAGTGAAGCAGAAAACCTTCTTAAACTTGGTACAGTGAGAAAACCGGAAGCTTATGATGGAATTTCAAAGACAAGACGGGAATTGTACCACATTAAAAATGGTAAAGGTTCCATTCCATACATCTCAGTCTTTCCAGTAAATCTGATTCTTGGTCTTGGCTCTTGTTTCTCAATTCTTTTGTTTGTGATATCTGGGGTTTATTCGAGTGTGAAGAATCTAAGGTTTATTAAGCTCAAAACCAATTTCTTCAAGAAAAATGGGGGCATATTGCTAGAGCAGCATATTGCTTCACATGGAGATGGAATGACAACAATATTTACAGCTGAGCAGCTAGAAATGGCTACTAACTGTTACCAAAGAATTTTACGCGATAAAGTAGTGGAGGGCCAAGTCAGGAGTTTTATAAATGAGATTATTGCTCTTGCCCAAATGAATCATGAGAATTTGGTGAAGTTCATTGGCTGCTGTTTAGAGACTGATGCTCCGATTTTGTTTATGAACTTGCCTCTAATGGAACCCTTTTCGACTGCATTAGTCACTCCAATGGGAAAGTTACGAGTTCACTCAAAACACATCATCCATGGCAATGTCAAGTCTTCCAACATATTGTTAACTGATAATTTCACAGCCAAAGTCTCTGGTTTTTGGCCTTCGAGGTTAGTTCCTGCTAATGAATCTCAGATGGGCACATTGCTTCTAAGGACACTAGGTTATATGGACCCAGGGTATCTTTGCATAGGCCAACTGACAGATAAGAGTGATGTTTATAGCTTCGGAGTCGTTTTGGTAGAGATCTTAACCCGGGAAAAGCCTATTTCATTTGACAGACCAGAAAGTCAAAGAATCATTGCTTCTCATTTTGTTTCATCCATTGAATTAAAAGATGGTTTCTTTCATGTTGTTGATCCACAAGTTTTAAACGAGGGAAATCGAGAACAAGTAAGAGCAGTTGCAGAGCTTGCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

1062

Amino Acids

118.16

Weight (kDa)

6.66

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 11 - 57 2.2e-09 PPR repeat family
PPR_2 PF13041 213 - 259 4.8e-08 PPR repeat family
PPR_1 PF12854 313 - 342 9.3e-06 PPR repeat
PPR_2 PF13041 316 - 361 1.4e-10 PPR repeat family
PPR PF01535 316 - 345 8.6e-08 PPR repeat
PPR_2 PF13041 415 - 462 1.4e-09 PPR repeat family
PPR_3 PF13812 416 - 461 2.4e-06 Pentatricopeptide repeat domain
PPR PF01535 417 - 446 2.6e-07 PPR repeat
E_motif PF20431 532 - 593 6.5e-17 E motif
Pkinase PF00069 863 - 1019 2.8e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 927 - 1010 1.1e-11 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2624
AasI GACNNNNNNGTC 1 cut(s) 3054
Acc36I ACCTGC 1 cut(s) 1382
AccB1I GGYRCC 1 cut(s) 2062
AccB7I CCANNNNNTGG 1 cut(s) 1603
AccII CGCG 2 cut(s) 1271, 2589
AciI CCGC 5 cut(s) 27, 665, 969, 1271, 1351
AclWI GGATC 4 cut(s) 569, 1108, 1121, 3119
AcoI YGGCCR 1 cut(s) 1677
AcuI CTGAAG 2 cut(s) 1851, 2146
AfaI GTAC 3 cut(s) 1384, 2251, 2306
AfiI CCNNNNNNNGG 3 cut(s) 997, 1556, 1603
AflIII ACRYGT 1 cut(s) 1179
AjnI CCWGG 4 cut(s) 1253, 1453, 1762, 2941
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 1031, 1063
Alw21I GWGCWC 2 cut(s) 1096, 1221
Alw26I GTCTC 7 cut(s) 649, 952, 1140, 1325, 1637, 2687, 2860
AlwI GGATC 4 cut(s) 569, 1108, 1121, 3119
Ama87I CYCGRG 3 cut(s) 1212, 1650, 3021
AoxI GGCC 4 cut(s) 1677, 2605, 2867, 2959
ApeKI GCWGC 7 cut(s) 29, 32, 1028, 1718, 2497, 2548, 2682
ArsI GACNNNNNNTTYG 2 cut(s) 398, 430
Asp700I GAANNNNTTC 1 cut(s) 2235
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 5 cut(s) 400, 796, 1309, 2605, 2938
AsuC2I CCSGG 5 cut(s) 421, 1651, 1652, 3022, 3023
AsuHPI GGTGA 7 cut(s) 213, 505, 905, 1046, 1586, 1625, 2680
AvaI CYCGRG 3 cut(s) 1212, 1650, 3021
AvaII GGWCC 4 cut(s) 400, 796, 1309, 2938
BaeGI GKGCMC 1 cut(s) 2909
BalI TGGCCA 1 cut(s) 1679
BamHI GGATCC 1 cut(s) 1113
BanI GGYRCC 1 cut(s) 2062
BanII GRGCYC 2 cut(s) 1221, 1650
BbsI GAAGAC 1 cut(s) 2808
Bbv12I GWGCWC 2 cut(s) 1096, 1221
BbvI GCAGC 7 cut(s) 16, 19, 1040, 1705, 2509, 2560, 2669
BccI CCATC 9 cut(s) 81, 722, 917, 1669, 1977, 2270, 2513, 2896, 3098
BceAI ACGGC 2 cut(s) 348, 1614
BciT130I CCWGG 4 cut(s) 1255, 1455, 1764, 2943
BciVI GTATCC 1 cut(s) 725
BclI TGATCA 2 cut(s) 963, 1099
BcnI CCSGG 5 cut(s) 421, 1651, 1652, 3022, 3023
BcoDI GTCTC 7 cut(s) 649, 952, 1140, 1325, 1637, 2687, 2860
BfaI CTAG 6 cut(s) 260, 909, 2112, 2492, 2552, 2927
BfoI RGCGCY 1 cut(s) 1004
BfuAI ACCTGC 1 cut(s) 1382
BfuI GTATCC 1 cut(s) 725
BglII AGATCT 2 cut(s) 1909, 3012
BisI GCNGC 7 cut(s) 30, 33, 1029, 1719, 2498, 2549, 2683
BlpI GCTNAGC 2 cut(s) 1886, 2544
BlsI GCNGC 7 cut(s) 31, 34, 1030, 1720, 2499, 2550, 2684
Bme1390I CCNGG 9 cut(s) 421, 1255, 1455, 1651, 1652, 1764, 2943, 3022, 3023
Bme18I GGWCC 4 cut(s) 400, 796, 1309, 2938
BmeT110I CYCGRG 3 cut(s) 1212, 1650, 3021
BmgT120I GGNCC 5 cut(s) 400, 796, 1309, 2605, 2938
BmiI GGNNCC 7 cut(s) 126, 989, 1115, 2064, 2329, 2736, 2940
BmrFI CCNGG 9 cut(s) 421, 1255, 1455, 1651, 1652, 1764, 2943, 3022, 3023
BmrI ACTGGG 1 cut(s) 842
BmsI GCATC 7 cut(s) 283, 339, 1314, 1543, 1700, 2111, 2689
BmuI ACTGGG 1 cut(s) 842
BoxI GACNNNNGTC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 2808
BpmI CTGGAG 1 cut(s) 1349
Bpu1102I GCTNAGC 2 cut(s) 1886, 2544
BpuEI CTTGAG 3 cut(s) 1025, 1421, 1658
BpuMI CCSGG 5 cut(s) 421, 1651, 1652, 3022, 3023
BsaWI WCCGGW 1 cut(s) 2263
BsaXI ACNNNNNCTCC 2 cut(s) 2686, 2716
Bsc4I CCNNNNNNNGG 3 cut(s) 997, 1556, 1603
Bse1I ACTGG 3 cut(s) 837, 1235, 2354
Bse3DI GCAATG 6 cut(s) 349, 1171, 1857, 2812, 2909, 3066
BseBI CCWGG 4 cut(s) 1255, 1455, 1764, 2943
BseGI GGATG 5 cut(s) 73, 1714, 1825, 2796, 3086
BseLI CCNNNNNNNGG 3 cut(s) 997, 1556, 1603
BseMI GCAATG 6 cut(s) 349, 1171, 1857, 2812, 2909, 3066
BseMII CTCAG 4 cut(s) 1877, 2358, 2535, 2912
BseNI ACTGG 3 cut(s) 837, 1235, 2354
BseSI GKGCMC 1 cut(s) 2909
BseXI GCAGC 7 cut(s) 16, 19, 1040, 1705, 2509, 2560, 2669
BsgI GTGCAG 1 cut(s) 386
Bsh1236I CGCG 2 cut(s) 1271, 2589
BshFI GGCC 4 cut(s) 1679, 2607, 2869, 2961
BshNI GGYRCC 1 cut(s) 2062
BsiHKAI GWGCWC 2 cut(s) 1096, 1221
BsiHKCI CYCGRG 3 cut(s) 1212, 1650, 3021
BsiSI CCGG 4 cut(s) 420, 1651, 2264, 3022
BslFI GGGAC 1 cut(s) 2065
BslI CCNNNNNNNGG 3 cut(s) 997, 1556, 1603
BsmAI GTCTC 7 cut(s) 649, 952, 1140, 1325, 1637, 2687, 2860
BsmBI CGTCTC 1 cut(s) 1637
BsmFI GGGAC 1 cut(s) 2065
BsmI GAATGC 1 cut(s) 539
BsnI GGCC 4 cut(s) 1679, 2607, 2869, 2961
BsoBI CYCGRG 3 cut(s) 1212, 1650, 3021
Bsp1286I GDGCHC 4 cut(s) 1096, 1221, 1650, 2909
Bsp143I GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
Bsp1720I GCTNAGC 2 cut(s) 1886, 2544
Bsp19I CCATGG 1 cut(s) 2800
BspACI CCGC 5 cut(s) 27, 665, 969, 1271, 1351
BspANI GGCC 4 cut(s) 1679, 2607, 2869, 2961
BspCNI CTCAG 4 cut(s) 1878, 2357, 2536, 2911
BspFNI CGCG 2 cut(s) 1271, 2589
BspHI TCATGA 1 cut(s) 2656
BspLI GGNNCC 7 cut(s) 126, 989, 1115, 2064, 2329, 2736, 2940
BspMI ACCTGC 1 cut(s) 1382
BspPI GGATC 4 cut(s) 569, 1108, 1121, 3119
BspQI GCTCTTC 1 cut(s) 1226
BspT107I GGYRCC 1 cut(s) 2062
BsrDI GCAATG 6 cut(s) 349, 1171, 1857, 2812, 2909, 3066
BsrI ACTGG 3 cut(s) 837, 1235, 2354
BssMI GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
BssT1I CCWWGG 3 cut(s) 600, 799, 2800
Bst2UI CCWGG 4 cut(s) 1255, 1455, 1764, 2943
Bst6I CTCTTC 1 cut(s) 1226
BstAPI GCANNNNNTGC 1 cut(s) 3179
BstC8I GCNNGC 4 cut(s) 929, 1558, 1585, 3180
BstDEI CTNAG 8 cut(s) 1577, 1886, 1973, 2344, 2438, 2544, 2898, 2918
BstDSI CCRYGG 1 cut(s) 2800
BstENI CCTNNNNNAGG 1 cut(s) 995
BstF5I GGATG 5 cut(s) 73, 1714, 1825, 2796, 3086
BstFNI CGCG 2 cut(s) 1271, 2589
BstH2I RGCGCY 1 cut(s) 1004
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 9 cut(s) 564, 966, 1102, 1116, 1269, 1465, 1912, 3015, 3127
BstMAI GTCTC 7 cut(s) 649, 952, 1140, 1325, 1637, 2687, 2860
BstMBI GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
BstMWI GCNNNNNNNGC 6 cut(s) 470, 1277, 1765, 1871, 2497, 3179
BstNI CCWGG 4 cut(s) 1255, 1455, 1764, 2943
BstNSI RCATGY 3 cut(s) 165, 931, 1183
BstPAI GACNNNNGTC 1 cut(s) 641
BstSCI CCNGG 9 cut(s) 419, 1253, 1453, 1649, 1650, 1762, 2941, 3020, 3021
BstSLI GKGCMC 1 cut(s) 2909
BstUI CGCG 2 cut(s) 1271, 2589
BstV1I GCAGC 7 cut(s) 16, 19, 1040, 1705, 2509, 2560, 2669
BstV2I GAAGAC 1 cut(s) 2808
BstX2I RGATCY 4 cut(s) 561, 1113, 1909, 3012
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 4 cut(s) 561, 1113, 1909, 3012
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 4 cut(s) 1679, 2607, 2869, 2961
BtgI CCRYGG 1 cut(s) 2800
BtgZI GCGATG 1 cut(s) 552
BtsCI GGATG 5 cut(s) 73, 1714, 1825, 2796, 3086
BtsI GCAGTG 2 cut(s) 1524, 2227
BtsIMutI CAGTG 5 cut(s) 1242, 1524, 1737, 2227, 2259
BveI ACCTGC 1 cut(s) 1382
Cac8I GCNNGC 4 cut(s) 929, 1558, 1585, 3180
CciI TCATGA 1 cut(s) 2656
CfoI GCGC 1 cut(s) 1003
Cfr13I GGNCC 5 cut(s) 400, 796, 1309, 2605, 2938
Cfr9I CCCGGG 2 cut(s) 1650, 3021
CseI GACGC 1 cut(s) 751
Csp6I GTAC 3 cut(s) 1383, 2250, 2305
CviQI GTAC 3 cut(s) 1383, 2250, 2305
DdeI CTNAG 8 cut(s) 1577, 1886, 1973, 2344, 2438, 2544, 2898, 2918
DpnI GATC 9 cut(s) 563, 965, 1101, 1115, 1268, 1464, 1911, 3014, 3126
DpnII GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
DraI TTTAAA 1 cut(s) 3138
DrdI GACNNNNNNGTC 1 cut(s) 3054
DseDI GACNNNNNNGTC 1 cut(s) 3054
EaeI YGGCCR 1 cut(s) 1677
Eam1104I CTCTTC 1 cut(s) 1226
EarI CTCTTC 1 cut(s) 1226
Ecl136II GAGCTC 1 cut(s) 1219
Eco130I CCWWGG 3 cut(s) 600, 799, 2800
Eco24I GRGCYC 2 cut(s) 1221, 1650
Eco32I GATATC 2 cut(s) 238, 2409
Eco47I GGWCC 4 cut(s) 400, 796, 1309, 2938
Eco53kI GAGCTC 1 cut(s) 1219
Eco57I CTGAAG 2 cut(s) 1851, 2146
Eco88I CYCGRG 3 cut(s) 1212, 1650, 3021
EcoICRI GAGCTC 1 cut(s) 1219
EcoNI CCTNNNNNAGG 1 cut(s) 995
EcoRI GAATTC 4 cut(s) 40, 516, 652, 825
EcoRII CCWGG 4 cut(s) 1253, 1453, 1762, 2941
EcoRV GATATC 2 cut(s) 238, 2409
EcoT14I CCWWGG 3 cut(s) 600, 799, 2800
EcoT22I ATGCAT 4 cut(s) 933, 1624, 1693, 2104
EcoT38I GRGCYC 2 cut(s) 1221, 1650
ErhI CCWWGG 3 cut(s) 600, 799, 2800
Esp3I CGTCTC 1 cut(s) 1637
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 2065
FauI CCCGC 3 cut(s) 658, 962, 1264
FauNDI CATATG 2 cut(s) 1562, 1666
FbaI TGATCA 2 cut(s) 963, 1099
Fnu4HI GCNGC 7 cut(s) 30, 33, 1029, 1719, 2498, 2549, 2683
FokI GGATG 5 cut(s) 60, 1721, 1812, 2783, 3073
FriOI GRGCYC 2 cut(s) 1221, 1650
Fsp4HI GCNGC 7 cut(s) 30, 33, 1029, 1719, 2498, 2549, 2683
FspBI CTAG 6 cut(s) 260, 909, 2112, 2492, 2552, 2927
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 7 cut(s) 30, 33, 1029, 1719, 2498, 2549, 2683
GsuI CTGGAG 1 cut(s) 1349
HaeII RGCGCY 1 cut(s) 1004
HaeIII GGCC 4 cut(s) 1679, 2607, 2869, 2961
HapII CCGG 4 cut(s) 420, 1651, 2264, 3022
HgaI GACGC 1 cut(s) 751
HhaI GCGC 1 cut(s) 1003
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HincII GTYRAC 1 cut(s) 2832
HindII GTYRAC 1 cut(s) 2832
HindIII AAGCTT 3 cut(s) 1377, 1757, 2268
HpaI GTTAAC 1 cut(s) 2832
HpaII CCGG 4 cut(s) 420, 1651, 2264, 3022
HphI GGTGA 7 cut(s) 213, 505, 905, 1046, 1586, 1625, 2680
Hpy166II GTNNAC 4 cut(s) 64, 709, 2782, 2832
Hpy8I GTNNAC 4 cut(s) 64, 709, 2782, 2832
HpyAV CCTTC 5 cut(s) 101, 157, 1695, 2246, 2880
HpyCH4IV ACGT 1 cut(s) 1495
HpyF10VI GCNNNNNNNGC 6 cut(s) 470, 1277, 1765, 1871, 2497, 3179
HpyF3I CTNAG 8 cut(s) 1577, 1886, 1973, 2344, 2438, 2544, 2898, 2918
HpySE526I ACGT 1 cut(s) 1495
HspAI GCGC 1 cut(s) 1001
Ksp22I TGATCA 2 cut(s) 963, 1099
KspAI GTTAAC 1 cut(s) 2832
Kzo9I GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
LguI GCTCTTC 1 cut(s) 1226
LmnI GCTCC 3 cut(s) 1091, 1522, 2707
Lsp1109I GCAGC 7 cut(s) 16, 19, 1040, 1705, 2509, 2560, 2669
LweI GCATC 7 cut(s) 283, 339, 1314, 1543, 1700, 2111, 2689
MaeI CTAG 6 cut(s) 260, 909, 2112, 2492, 2552, 2927
MaeII ACGT 1 cut(s) 1495
MaeIII GTNAC 7 cut(s) 1051, 1237, 1572, 2008, 2570, 2755, 2772
MalI GATC 9 cut(s) 563, 965, 1101, 1115, 1268, 1464, 1911, 3014, 3126
MboI GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 4 cut(s) 561, 1113, 1909, 3012
MhlI GDGCHC 4 cut(s) 1096, 1221, 1650, 2909
MlsI TGGCCA 1 cut(s) 1679
MluNI TGGCCA 1 cut(s) 1679
MlyI GAGTC 3 cut(s) 602, 2089, 3006
MmeI TCCRAC 2 cut(s) 201, 2844
Mox20I TGGCCA 1 cut(s) 1679
Mph1103I ATGCAT 4 cut(s) 933, 1624, 1693, 2104
MroXI GAANNNNTTC 1 cut(s) 2235
MscI TGGCCA 1 cut(s) 1679
MslI CAYNNNNRTG 8 cut(s) 207, 629, 1122, 1785, 1974, 2517, 2762, 2805
Msp20I TGGCCA 1 cut(s) 1679
MspA1I CMGCKG 4 cut(s) 29, 969, 1031, 2543
MspI CCGG 4 cut(s) 420, 1651, 2264, 3022
MspR9I CCNGG 9 cut(s) 421, 1255, 1455, 1651, 1652, 1764, 2943, 3022, 3023
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 539
MvaI CCWGG 4 cut(s) 1255, 1455, 1764, 2943
MvnI CGCG 2 cut(s) 1271, 2589
MwoI GCNNNNNNNGC 6 cut(s) 470, 1277, 1765, 1871, 2497, 3179
NciI CCSGG 5 cut(s) 421, 1651, 1652, 3022, 3023
NcoI CCATGG 1 cut(s) 2800
NdeI CATATG 2 cut(s) 1562, 1666
NdeII GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
NlaIV GGNNCC 7 cut(s) 126, 989, 1115, 2064, 2329, 2736, 2940
NmuCI GTSAC 2 cut(s) 1237, 2755
NsiI ATGCAT 4 cut(s) 933, 1624, 1693, 2104
NspI RCATGY 3 cut(s) 165, 931, 1183
PaeI GCATGC 1 cut(s) 931
PaeR7I CTCGAG 1 cut(s) 1212
PagI TCATGA 1 cut(s) 2656
PasI CCCWGGG 1 cut(s) 2942
PciI ACATGT 1 cut(s) 1179
PciSI GCTCTTC 1 cut(s) 1226
PctI GAATGC 1 cut(s) 539
PdmI GAANNNNTTC 1 cut(s) 2235
PflMI CCANNNNNTGG 1 cut(s) 1603
PfoI TCCNGGA 1 cut(s) 1253
PkrI GCNGC 7 cut(s) 31, 34, 1030, 1720, 2499, 2550, 2684
PleI GAGTC 3 cut(s) 601, 2089, 3005
PpsI GAGTC 3 cut(s) 601, 2089, 3005
PscI ACATGT 1 cut(s) 1179
PshAI GACNNNNGTC 1 cut(s) 641
PsiI TTATAA 1 cut(s) 2624
Psp124BI GAGCTC 1 cut(s) 1221
Psp6I CCWGG 4 cut(s) 1253, 1453, 1762, 2941
PspGI CCWGG 4 cut(s) 1253, 1453, 1762, 2941
PspN4I GGNNCC 7 cut(s) 126, 989, 1115, 2064, 2329, 2736, 2940
PspPI GGNCC 5 cut(s) 400, 796, 1309, 2605, 2938
PsuI RGATCY 4 cut(s) 561, 1113, 1909, 3012
PvuII CAGCTG 2 cut(s) 1031, 2543
RsaI GTAC 3 cut(s) 1384, 2251, 2306
RsaNI GTAC 3 cut(s) 1383, 2250, 2305
RseI CAYNNNNRTG 8 cut(s) 207, 629, 1122, 1785, 1974, 2517, 2762, 2805
SacI GAGCTC 1 cut(s) 1221
SapI GCTCTTC 1 cut(s) 1226
SatI GCNGC 7 cut(s) 30, 33, 1029, 1719, 2498, 2549, 2683
Sau3AI GATC 9 cut(s) 561, 963, 1099, 1113, 1266, 1462, 1909, 3012, 3124
Sau96I GGNCC 5 cut(s) 400, 796, 1309, 2605, 2938
SchI GAGTC 3 cut(s) 602, 2089, 3006
ScrFI CCNGG 9 cut(s) 421, 1255, 1455, 1651, 1652, 1764, 2943, 3022, 3023
SduI GDGCHC 4 cut(s) 1096, 1221, 1650, 2909
SfaNI GCATC 7 cut(s) 283, 339, 1314, 1543, 1700, 2111, 2689
Sfr274I CTCGAG 1 cut(s) 1212
SinI GGWCC 4 cut(s) 400, 796, 1309, 2938
SlaI CTCGAG 1 cut(s) 1212
SmaI CCCGGG 2 cut(s) 1652, 3023
SmiMI CAYNNNNRTG 8 cut(s) 207, 629, 1122, 1785, 1974, 2517, 2762, 2805
SmlI CTYRAG 4 cut(s) 1004, 1212, 1400, 1637
SmoI CTYRAG 4 cut(s) 1004, 1212, 1400, 1637
SphI GCATGC 1 cut(s) 931
SsiI CCGC 5 cut(s) 27, 665, 969, 1271, 1351
SspI AATATT 1 cut(s) 2535
SspMI CTAG 6 cut(s) 260, 909, 2112, 2492, 2552, 2927
SstI GAGCTC 1 cut(s) 1221
StyD4I CCNGG 9 cut(s) 419, 1253, 1453, 1649, 1650, 1762, 2941, 3020, 3021
StyI CCWWGG 3 cut(s) 600, 799, 2800
TaiI ACGT 1 cut(s) 1498
TaqI TCGA 8 cut(s) 309, 656, 921, 1213, 2423, 2744, 2873, 3151
TatI WGTACW 1 cut(s) 1382
TscAI CASTG 5 cut(s) 1242, 1524, 1737, 2227, 2259
TseFI GTSAC 2 cut(s) 1237, 2755
TseI GCWGC 7 cut(s) 29, 32, 1028, 1718, 2497, 2548, 2682
Tsp45I GTSAC 2 cut(s) 1237, 2755
TspMI CCCGGG 2 cut(s) 1650, 3021
TspRI CASTG 5 cut(s) 1242, 1524, 1737, 2227, 2259
Van91I CCANNNNNTGG 1 cut(s) 1603
VpaK11BI GGWCC 4 cut(s) 400, 796, 1309, 2938
XagI CCTNNNNNAGG 1 cut(s) 995
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 3 cut(s) 165, 931, 1183
XcmI CCANNNNNNNNNTGG 2 cut(s) 1901, 2315
XhoI CTCGAG 1 cut(s) 1212
XmaI CCCGGG 2 cut(s) 1650, 3021
XmnI GAANNNNTTC 1 cut(s) 2235
XspI CTAG 6 cut(s) 260, 909, 2112, 2492, 2552, 2927
Zsp2I ATGCAT 4 cut(s) 933, 1624, 1693, 2104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.