Rw0G011160

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00483
OG0004833
Physical Location & Seq
Forward (+)
5998 .. 7433
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G011160.1

Sequence Viewer

Length: 357 bp
CCGCTGTCCCTAGAAGCCTCCGACCCCCTCCACGATGCCATTGAAGTGAACGACGAGTTGTGCGTCGCGGACGCGATTAGGAACCTCTGCCAGATCTTTCCGTTAAGCCCTTCATCAATGGCGGAGTCTCTGGTATGCTCGCTCCCTGCGTCATCCAACCCATCGATACGATCAAGCTTCCTGGAGCGCTCAGCCATAGGCTACAGGGACAGACCCTCTATTATGTATGGAGACATCGTCTACACTTGGAGACAGACACTTGAACGATGCACCAGACTTGCTTCTTCTCTTGCCCAACTTGGAATTTCTCAAGGCGATGTGAATGCATTTGAGCTCGCCTTCTTCGTTTGGGTTCCA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

119

Amino Acids

13.11

Weight (kDa)

4.53

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 61 - 107 3.7e-06 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 68, 74
AciI CCGC 3 cut(s) 2, 68, 122
AcsI RAATTY 1 cut(s) 303
AfeI AGCGCT 1 cut(s) 188
AgsI TTSAA 2 cut(s) 44, 263
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 2 cut(s) 177, 334
AluI AGCT 2 cut(s) 177, 334
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 3 cut(s) 132, 225, 244
Aor51HI AGCGCT 1 cut(s) 188
ApoI RAATTY 1 cut(s) 303
AspLEI GCGC 1 cut(s) 189
BanII GRGCYC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 336
BccI CCATC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 182
BcoDI GTCTC 3 cut(s) 132, 225, 244
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 202
BfoI RGCGCY 1 cut(s) 190
BglII AGATCT 1 cut(s) 93
BlpI GCTNAGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 83, 354
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 2 cut(s) 25, 257
BpmI CTGGAG 1 cut(s) 203
Bpu1102I GCTNAGC 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 164
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
BseBI CCWGG 1 cut(s) 182
BseCI ATCGAT 1 cut(s) 164
BseGI GGATG 1 cut(s) 152
BseMII CTCAG 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 68, 74
BshVI ATCGAT 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 336
BslFI GGGAC 1 cut(s) 221
BsmAI GTCTC 3 cut(s) 132, 225, 244
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 2 cut(s) 93, 170
Bsp1720I GCTNAGC 1 cut(s) 190
BspACI CCGC 3 cut(s) 2, 68, 122
BspCNI CTCAG 1 cut(s) 203
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 2 cut(s) 68, 74
BspLI GGNNCC 2 cut(s) 83, 354
BssMI GATC 2 cut(s) 93, 170
Bst2UI CCWGG 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 140, 336
BstDEI CTNAG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 2 cut(s) 68, 74
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 2 cut(s) 96, 173
BstMAI GTCTC 3 cut(s) 132, 225, 244
BstMBI GATC 2 cut(s) 93, 170
BstNI CCWGG 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 202
BstUI CGCG 2 cut(s) 68, 74
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
Bsu15I ATCGAT 1 cut(s) 164
BsuTUI ATCGAT 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 140, 336
CfoI GCGC 1 cut(s) 189
ClaI ATCGAT 1 cut(s) 164
CseI GACGC 3 cut(s) 52, 80, 138
CviJI RGCY 6 cut(s) 17, 108, 177, 194, 201, 334
CviKI_1 RGCY 6 cut(s) 17, 108, 177, 194, 201, 334
DdeI CTNAG 1 cut(s) 190
DpnI GATC 2 cut(s) 95, 172
DpnII GATC 2 cut(s) 93, 170
EciI GGCGGA 1 cut(s) 137
Ecl136II GAGCTC 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 336
Eco47III AGCGCT 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 334
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 336
FaiI YATR 4 cut(s) 136, 197, 224, 228
FaqI GGGAC 1 cut(s) 221
FblI GTMKAC 1 cut(s) 240
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 336
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 188
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 190
HgaI GACGC 3 cut(s) 52, 80, 138
HhaI GCGC 1 cut(s) 189
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 49, 241
Hpy188I TCNGA 1 cut(s) 22
Hpy8I GTNNAC 2 cut(s) 49, 241
Hpy99I CGWCG 2 cut(s) 56, 68
HpyAV CCTTC 2 cut(s) 120, 349
HpyCH4V TGCA 2 cut(s) 270, 326
HpyF3I CTNAG 1 cut(s) 190
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 2 cut(s) 93, 170
LmnI GCTCC 2 cut(s) 147, 184
LpnPI CCDG 7 cut(s) 104, 116, 159, 167, 190, 194, 286
LweI GCATC 2 cut(s) 25, 257
MaeI CTAG 1 cut(s) 11
MalI GATC 2 cut(s) 95, 172
MboI GATC 2 cut(s) 93, 170
MboII GAAGA 2 cut(s) 276, 334
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 1 cut(s) 303
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 45, 180
MnlI CCTC 4 cut(s) 28, 38, 95, 226
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 4
MspR9I CCNGG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 2 cut(s) 68, 74
NdeII GATC 2 cut(s) 93, 170
NlaIV GGNNCC 2 cut(s) 83, 354
NsiI ATGCAT 1 cut(s) 328
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 146, 342
PctI GAATGC 1 cut(s) 328
PflFI GACNNNGTC 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 180
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 2 cut(s) 83, 354
PsuI RGATCY 1 cut(s) 93
PsyI GACNNNGTC 1 cut(s) 236
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 104
Sau3AI GATC 2 cut(s) 93, 170
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 336
SetI ASST 3 cut(s) 87, 179, 336
SfaNI GCATC 2 cut(s) 25, 257
SfcI CTRYAG 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
Sse9I AATT 1 cut(s) 303
SsiI CCGC 3 cut(s) 2, 68, 122
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 180
TaqI TCGA 1 cut(s) 164
TasI AATT 1 cut(s) 303
Tru1I TTAA 1 cut(s) 104
Tru9I TTAA 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 1 cut(s) 90
Tth111I GACNNNGTC 1 cut(s) 236
XapI RAATTY 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.