MD02G1120100.v1.1

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004833
Physical Location & Seq
Reverse (-)
9763125 .. 9767456
4332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1120100.v1.1.491

Sequence Viewer

Length: 759 bp
ATGAATAAGGCTAGTCTTGTTCAGTGCTCGGCAAACTACATACCCTTGTCACCTATAAGCTTCTTAGAGCGAGCTGCTGTTGTCCACGGCGATAAGATTTCGATCGTTTACGGTGGAGTGAGATTTTCATGGCAGCAGACGCTTGAAAGATGTCGCAAGGTCGCCTCAGCTTTCGTCCACTTAGGCATATGTCGCCATGATATTGTTGCAGCTTTTGCACCAAACATTCCAGCACTTTATGAGCTACATTTCAGCGTTCCAATGGCTGGAGCAGTTCTTTGTGCACTTAACATTAAGCTGGACGCACCCGCATTAGCGTTGATATTACAACAACTGGAAGCCAAAGTCATTATTGTAGATCATCAGTACCTTAAAGTTGTCCTCCAATCACTTGACATTTTGTCCCAAACAAAGTCCAACTCCAAGCCACCTCTCCTCATTCAAATCCCAAAGTGTGGCCAATTATCATCATCTTCTCCTACTGATCTTGATCTTCCACCAGATGCCCTTAATTACAATGACCTGCAGGAATCAGCAGAACCCAGTTTTCAGATTCTCAGACCCAACAATGAATGTGATCCAATTTCGGTGAGTTACACTTCTGGGTCAACAGGTGATCCCAAGGGAGTTGTGTACAGCCACAGAGCGGTGTACCTAAATTCACTGGGAGCAATTTTTCAGAGTGACATGAGAAAAATGCCGGTTTTTCTATGGACGGTCGAAATGTTTCGGTGCAATGGCTGCCAAAGCCATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

253

Amino Acids

27.72

Weight (kDa)

7.02

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 22 - 248 8.6e-37 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 531
AccB7I CCANNNNNTGG 2 cut(s) 266, 455
AccBSI CCGCTC 1 cut(s) 647
AciI CCGC 2 cut(s) 309, 647
AclWI GGATC 2 cut(s) 572, 611
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 1 cut(s) 658
AfaI GTAC 3 cut(s) 368, 635, 653
AfiI CCNNNNNNNGG 3 cut(s) 266, 455, 646
AgsI TTSAA 2 cut(s) 146, 443
AhdI GACNNNNNGTC 1 cut(s) 400
AluBI AGCT 6 cut(s) 60, 74, 170, 212, 244, 298
AluI AGCT 6 cut(s) 60, 74, 170, 212, 244, 298
Alw21I GWGCWC 2 cut(s) 29, 286
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 2 cut(s) 572, 611
AoxI GGCC 1 cut(s) 457
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 4 cut(s) 74, 133, 209, 741
ApoI RAATTY 1 cut(s) 658
Asp700I GAANNNNTTC 1 cut(s) 726
AsuHPI GGTGA 3 cut(s) 42, 601, 626
BaeGI GKGCMC 1 cut(s) 286
BalI TGGCCA 1 cut(s) 459
Bbv12I GWGCWC 2 cut(s) 29, 286
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 4 cut(s) 61, 145, 221, 728
BceAI ACGGC 1 cut(s) 103
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 531
BisI GCNGC 4 cut(s) 75, 134, 210, 742
BlsI GCNGC 4 cut(s) 76, 135, 211, 743
BmeRI GACNNNNNGTC 1 cut(s) 400
BmrI ACTGGG 2 cut(s) 537, 674
BmsI GCATC 1 cut(s) 493
BmuI ACTGGG 2 cut(s) 537, 674
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 166
BsaBI GATNNNNATC 2 cut(s) 101, 489
BsaJI CCNNGG 2 cut(s) 85, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 455, 646
Bse118I RCCGGY 1 cut(s) 700
Bse1I ACTGG 3 cut(s) 339, 543, 669
Bse3DI GCAATG 1 cut(s) 742
Bse8I GATNNNNATC 2 cut(s) 101, 489
BseDI CCNNGG 2 cut(s) 85, 621
BseJI GATNNNNATC 2 cut(s) 101, 489
BseLI CCNNNNNNNGG 3 cut(s) 266, 455, 646
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 2 cut(s) 180, 571
BseNI ACTGG 3 cut(s) 339, 543, 669
BseRI GAGGAG 1 cut(s) 425
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 4 cut(s) 61, 145, 221, 728
Bsh1285I CGRYCG 2 cut(s) 105, 720
BshFI GGCC 1 cut(s) 459
BsiEI CGRYCG 2 cut(s) 105, 720
BsiHKAI GWGCWC 2 cut(s) 29, 286
BsiSI CCGG 1 cut(s) 701
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 3 cut(s) 266, 455, 646
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 1 cut(s) 459
Bsp1286I GDGCHC 2 cut(s) 29, 286
Bsp1407I TGTACA 1 cut(s) 633
Bsp143I GATC 6 cut(s) 102, 358, 484, 490, 577, 616
BspACI CCGC 2 cut(s) 309, 647
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 2 cut(s) 179, 570
BspMAI CTGCAG 1 cut(s) 528
BspMI ACCTGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 572, 611
BsrBI CCGCTC 1 cut(s) 647
BsrDI GCAATG 1 cut(s) 742
BsrFI RCCGGY 1 cut(s) 700
BsrGI TGTACA 1 cut(s) 633
BsrI ACTGG 3 cut(s) 339, 543, 669
BssAI RCCGGY 1 cut(s) 700
BssECI CCNNGG 2 cut(s) 85, 621
BssMI GATC 6 cut(s) 102, 358, 484, 490, 577, 616
BssT1I CCWWGG 1 cut(s) 621
Bst4CI ACNGT 2 cut(s) 113, 718
BstAPI GCANNNNNTGC 2 cut(s) 215, 741
BstAUI TGTACA 1 cut(s) 633
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 4 cut(s) 64, 166, 181, 557
BstDSI CCRYGG 1 cut(s) 85
BstKTI GATC 6 cut(s) 105, 361, 487, 493, 580, 619
BstMBI GATC 6 cut(s) 102, 358, 484, 490, 577, 616
BstMCI CGRYCG 2 cut(s) 105, 720
BstMWI GCNNNNNNNGC 5 cut(s) 139, 192, 215, 741, 747
BstSFI CTRYAG 1 cut(s) 524
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 4 cut(s) 61, 145, 221, 728
BsuRI GGCC 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 85
BtsIMutI CAGTG 2 cut(s) 29, 662
BveI ACCTGC 1 cut(s) 531
Cac8I GCNNGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 700
CseI GACGC 2 cut(s) 148, 311
Csp6I GTAC 3 cut(s) 367, 634, 652
CviAII CATG 3 cut(s) 129, 197, 688
CviQI GTAC 3 cut(s) 367, 634, 652
DdeI CTNAG 4 cut(s) 64, 166, 181, 557
DpnI GATC 6 cut(s) 104, 360, 486, 492, 579, 618
DpnII GATC 6 cut(s) 102, 358, 484, 490, 577, 616
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 457
Eam1105I GACNNNNNGTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 621
EcoT14I CCWWGG 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 3 cut(s) 132, 200, 691
FaiI YATR 9 cut(s) 41, 56, 130, 188, 190, 198, 240, 689, 712
FaqI GGGAC 1 cut(s) 388
FatI CATG 3 cut(s) 128, 196, 687
FauI CCCGC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 188
Fnu4HI GCNGC 4 cut(s) 75, 134, 210, 742
Fsp4HI GCNGC 4 cut(s) 75, 134, 210, 742
FspBI CTAG 1 cut(s) 12
GluI GCNGC 4 cut(s) 75, 134, 210, 742
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 1 cut(s) 459
HapII CCGG 1 cut(s) 701
HgaI GACGC 2 cut(s) 148, 311
Hin1II CATG 3 cut(s) 132, 200, 691
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 2 cut(s) 530, 553
HpaII CCGG 1 cut(s) 701
HphI GGTGA 3 cut(s) 42, 601, 626
Hpy166II GTNNAC 7 cut(s) 85, 109, 178, 284, 609, 634, 652
Hpy188I TCNGA 3 cut(s) 552, 560, 681
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 7 cut(s) 85, 109, 178, 284, 609, 634, 652
HpyCH4III ACNGT 2 cut(s) 113, 718
HpyCH4V TGCA 5 cut(s) 209, 218, 284, 526, 735
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 192, 215, 741, 747
HpyF3I CTNAG 4 cut(s) 64, 166, 181, 557
Hsp92II CATG 3 cut(s) 132, 200, 691
Kzo9I GATC 6 cut(s) 102, 358, 484, 490, 577, 616
LmnI GCTCC 2 cut(s) 269, 668
Lsp1109I GCAGC 4 cut(s) 61, 145, 221, 728
LweI GCATC 1 cut(s) 493
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 3 cut(s) 48, 593, 683
MalI GATC 6 cut(s) 104, 360, 486, 492, 579, 618
MbiI CCGCTC 1 cut(s) 647
MboI GATC 6 cut(s) 102, 358, 484, 490, 577, 616
MboII GAAGA 2 cut(s) 465, 485
MhlI GDGCHC 2 cut(s) 29, 286
MlsI TGGCCA 1 cut(s) 459
MluCI AATT 5 cut(s) 461, 511, 582, 658, 672
MluNI TGGCCA 1 cut(s) 459
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 4 cut(s) 175, 392, 441, 446
Mox20I TGGCCA 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 726
MscI TGGCCA 1 cut(s) 459
MseI TTAA 4 cut(s) 288, 294, 372, 510
Msp20I TGGCCA 1 cut(s) 459
MspI CCGG 1 cut(s) 701
MwoI GCNNNNNNNGC 5 cut(s) 139, 192, 215, 741, 747
NdeI CATATG 1 cut(s) 188
NdeII GATC 6 cut(s) 102, 358, 484, 490, 577, 616
NlaIII CATG 3 cut(s) 132, 200, 691
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 48, 683
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 2 cut(s) 530, 553
PflMI CCANNNNNTGG 2 cut(s) 266, 455
PkrI GCNGC 4 cut(s) 76, 135, 211, 743
Ple19I CGATCG 1 cut(s) 105
PstI CTGCAG 1 cut(s) 528
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 3 cut(s) 368, 635, 653
RsaNI GTAC 3 cut(s) 367, 634, 652
SaqAI TTAA 4 cut(s) 288, 294, 372, 510
SatI GCNGC 4 cut(s) 75, 134, 210, 742
Sau3AI GATC 6 cut(s) 102, 358, 484, 490, 577, 616
SbfI CCTGCAGG 1 cut(s) 528
SdaI CCTGCAGG 1 cut(s) 528
SduI GDGCHC 2 cut(s) 29, 286
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 1 cut(s) 524
Sse8387I CCTGCAGG 1 cut(s) 528
Sse9I AATT 5 cut(s) 461, 511, 582, 658, 672
SsiI CCGC 2 cut(s) 309, 647
SspMI CTAG 1 cut(s) 12
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 2 cut(s) 113, 718
TaqI TCGA 2 cut(s) 101, 720
TasI AATT 5 cut(s) 461, 511, 582, 658, 672
TatI WGTACW 1 cut(s) 633
TfiI GAWTC 2 cut(s) 530, 553
Tru1I TTAA 4 cut(s) 288, 294, 372, 510
Tru9I TTAA 4 cut(s) 288, 294, 372, 510
TscAI CASTG 2 cut(s) 29, 669
TseFI GTSAC 2 cut(s) 48, 683
TseI GCWGC 4 cut(s) 74, 133, 209, 741
Tsp45I GTSAC 2 cut(s) 48, 683
TspDTI ATGAA 3 cut(s) 17, 117, 585
TspRI CASTG 2 cut(s) 29, 669
Van91I CCANNNNNTGG 2 cut(s) 266, 455
VneI GTGCAC 1 cut(s) 282
XapI RAATTY 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.