Rh2AG114700

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004833
Physical Location & Seq
Reverse (-)
10251589 .. 10258826
7238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG114700.1

Sequence Viewer

Length: 1401 bp
ATGGCCGGAGCAATTCTTTGTGCCCTCAACATTAGGCTGGACTCACCCACATTATCATTGATATTACAACAGTTGGAGGCCAAAGTCATTCTCGTAGACTATCAGTATCTTCAGGTTGTCCTCCAAGCACTCGAAAAATTGTCCCCAAAAATATGCAAGCCACCTCTACTCATTCTCATCCCAGAGTGCGACCAACTCTCATCTAATGGTACCATAGATCATGACCTTCCACCAGGTAGCTTGAATTATAACGACCTGCAGGGTAGTACAGCGGAACCCGACTTTGAGATTCGAAGACCCCTCAATGAATGTGACCCAATTTCAGTGAACTACACTTCTGGTTCAACTGGGAATCCCAAAGGAGTAGTGTACAGCCACAGAGCTACGTACCTGAATTCACTAGGAGCAATTTTTCAGAGTGAAATGGCAAAAATGCCAGTGTTCCTATGGACAGTTGACATGTTTCGCTGCAATGGATGGTGCTTCCCTTGGGCTGTTGCTGCTCTTGGGGGAACCAATATCTTCCTCAGAGAAAATCACTCCGCAAAGCTCATTTTCGAAGCGATTGATCTTCACAAGGTAATTCATTTGTGTGGATCACCCTGCATTTTAAACATTCTAGCACATCATGCCTCAAAAAGTGACCAAATTAGGCCATTGAAATCAACGGTGAATATTCTTGTTGCCGGTGCATTGCCACCGCCGCAGATTCTTAATCAAGTTGCCAAATTGGGTTTCAATGTGAGTCATGGATATGGTATGACAGAGGCTCTTGGTCCAGCAATTGTTACACCGTGTAAACCAGAACCGGAGTCCCAGTACAATTTGATGATGGAAGGGGTTGATGTGAAGGACCCAAGCACCATGGAAAGTGTTCCATATGATGGTAAAACAATGGGGGAGATAATGTTTAGGGGCAACACTTTGATGTTGGGGTACCTCAAAAATTCAAAAGCAATGATTGAGCCTTTTCAGGGTGGGTGGTACAGAACTGGTGACCTAGCAATCAGACACTCAGATGGGTACATTCAAATGAAGGATAGGGCAAGGGACATGATCATTTCTGGGGGGGAGGCTATAAGTACACTTGAGGTAGAGGCAGTTTTGTTGACTCATCCTTGTGTTTTGGAGGCTGCTGTTGTGGGGAGAAATGATGATTGCTTGGGGAAGACTCCTTGTGCTTTTGTGAAGTTGAAGGAGGGATTTTGCAGTGGTGGAAATTCAAGTACTTCAGAGGAGATTATGAAGTTTTGTGGGGAGAAATTGCCTGCTTATATGGTTCCAAAAGCTGTGGTTTTCGGGGATCTGCCATTGAATTCTACTGGGAAGATACAAAAGTTTGTTCTGAGGGAGAAGGCAAATAAACAGGTTTGTGCTGAGACCACTATGTTACATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

466

Amino Acids

50.98

Weight (kDa)

5.92

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 314 1.3e-46 AMP-binding enzyme
AMP-binding_C PF13193 364 - 443 3.4e-18 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 264
Acc65I GGTACC 2 cut(s) 209, 936
AccB1I GGYRCC 2 cut(s) 209, 936
AccB7I CCANNNNNTGG 1 cut(s) 884
AccI GTMKAC 1 cut(s) 96
AciI CCGC 4 cut(s) 272, 543, 701, 704
AclWI GGATC 2 cut(s) 604, 1311
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 394, 946, 1219, 1315
AcuI CTGAAG 2 cut(s) 95, 1215
AfiI CCNNNNNNNGG 2 cut(s) 884, 974
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 9 cut(s) 244, 345, 661, 739, 951, 1031, 1195, 1224, 1315
AjnI CCWGG 1 cut(s) 232
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
AluBI AGCT 4 cut(s) 240, 383, 550, 1289
AluI AGCT 4 cut(s) 240, 383, 550, 1289
Alw26I GTCTC 1 cut(s) 1373
AlwI GGATC 2 cut(s) 604, 1311
AoxI GGCC 3 cut(s) 3, 78, 653
ApeKI GCWGC 3 cut(s) 468, 500, 1133
ApoI RAATTY 4 cut(s) 394, 946, 1219, 1315
Asp700I GAANNNNTTC 1 cut(s) 873
Asp718I GGTACC 2 cut(s) 209, 936
AspS9I GGNCC 2 cut(s) 776, 853
AsuHPI GGTGA 4 cut(s) 36, 591, 682, 1007
AsuII TTCGAA 2 cut(s) 292, 558
AvaII GGWCC 2 cut(s) 776, 853
BaeGI GKGCMC 1 cut(s) 25
BaeI ACNNNNGTAYC 2 cut(s) 1322, 1355
BanI GGYRCC 2 cut(s) 209, 936
BbsI GAAGAC 2 cut(s) 301, 1175
BbvI GCAGC 3 cut(s) 455, 487, 1120
BccI CCATC 4 cut(s) 471, 826, 878, 1013
BciT130I CCWGG 1 cut(s) 234
BclI TGATCA 1 cut(s) 1056
BcoDI GTCTC 1 cut(s) 1373
BfaI CTAG 3 cut(s) 401, 620, 1001
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 264
BisI GCNGC 4 cut(s) 469, 501, 704, 1134
BlsI GCNGC 4 cut(s) 470, 502, 705, 1135
BmcAI AGTACT 1 cut(s) 1228
Bme1390I CCNGG 1 cut(s) 234
Bme18I GGWCC 2 cut(s) 776, 853
BmgT120I GGNCC 2 cut(s) 776, 853
BmiI GGNNCC 6 cut(s) 211, 276, 514, 855, 938, 1281
BmrFI CCNGG 1 cut(s) 234
BmrI ACTGGG 3 cut(s) 357, 811, 1332
BmuI ACTGGG 3 cut(s) 357, 811, 1332
BpiI GAAGAC 2 cut(s) 301, 1175
Bpu14I TTCGAA 2 cut(s) 292, 558
BpuEI CTTGAG 1 cut(s) 1109
BsaAI YACGTR 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 1373
BsaJI CCNNGG 2 cut(s) 488, 864
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 4 cut(s) 803, 833, 893, 923
Bsc4I CCNNNNNNNGG 2 cut(s) 884, 974
Bse118I RCCGGY 1 cut(s) 686
Bse1I ACTGG 5 cut(s) 352, 437, 817, 997, 1327
Bse3DI GCAATG 3 cut(s) 478, 692, 963
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 488, 864
BseGI GGATG 3 cut(s) 177, 482, 1114
BseLI CCNNNNNNNGG 2 cut(s) 884, 974
BseMI GCAATG 3 cut(s) 478, 692, 963
BseMII CTCAG 4 cut(s) 541, 1029, 1337, 1368
BseNI ACTGG 5 cut(s) 352, 437, 817, 997, 1327
BseRI GAGGAG 1 cut(s) 1250
BseSI GKGCMC 1 cut(s) 25
BseXI GCAGC 3 cut(s) 455, 487, 1120
BshFI GGCC 3 cut(s) 5, 80, 655
BshNI GGYRCC 2 cut(s) 209, 936
BsiSI CCGG 3 cut(s) 6, 687, 809
BslFI GGGAC 3 cut(s) 127, 799, 1064
BslI CCNNNNNNNGG 2 cut(s) 884, 974
BsmAI GTCTC 1 cut(s) 1373
BsmFI GGGAC 3 cut(s) 127, 799, 1064
BsnI GGCC 3 cut(s) 5, 80, 655
Bso31I GGTCTC 1 cut(s) 1373
Bsp119I TTCGAA 2 cut(s) 292, 558
Bsp1286I GDGCHC 1 cut(s) 25
Bsp1407I TGTACA 1 cut(s) 369
Bsp143I GATC 5 cut(s) 217, 568, 596, 1056, 1303
Bsp19I CCATGG 1 cut(s) 864
BspACI CCGC 4 cut(s) 272, 543, 701, 704
BspANI GGCC 3 cut(s) 5, 80, 655
BspCNI CTCAG 4 cut(s) 540, 1028, 1338, 1369
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 6 cut(s) 211, 276, 514, 855, 938, 1281
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 2 cut(s) 604, 1311
BspT104I TTCGAA 2 cut(s) 292, 558
BspT107I GGYRCC 2 cut(s) 209, 936
BspTNI GGTCTC 1 cut(s) 1373
BsrDI GCAATG 3 cut(s) 478, 692, 963
BsrFI RCCGGY 1 cut(s) 686
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 5 cut(s) 352, 437, 817, 997, 1327
BssAI RCCGGY 1 cut(s) 686
BssECI CCNNGG 2 cut(s) 488, 864
BssMI GATC 5 cut(s) 217, 568, 596, 1056, 1303
BssT1I CCWWGG 2 cut(s) 488, 864
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 4 cut(s) 72, 454, 670, 795
BstAPI GCANNNNNTGC 1 cut(s) 629
BstAUI TGTACA 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 387
BstBI TTCGAA 2 cut(s) 292, 558
BstC8I GCNNGC 2 cut(s) 158, 1269
BstDEI CTNAG 4 cut(s) 527, 1015, 1346, 1377
BstDSI CCRYGG 1 cut(s) 864
BstEII GGTNACC 1 cut(s) 995
BstF5I GGATG 3 cut(s) 177, 482, 1114
BstKTI GATC 5 cut(s) 220, 571, 599, 1059, 1306
BstMAI GTCTC 1 cut(s) 1373
BstMBI GATC 5 cut(s) 217, 568, 596, 1056, 1303
BstMWI GCNNNNNNNGC 3 cut(s) 500, 629, 703
BstNI CCWGG 1 cut(s) 234
BstNSI RCATGY 1 cut(s) 463
BstPI GGTNACC 1 cut(s) 995
BstSCI CCNGG 1 cut(s) 232
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 25
BstSNI TACGTA 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 455, 487, 1120
BstV2I GAAGAC 2 cut(s) 301, 1175
BstX2I RGATCY 1 cut(s) 1303
BstYI RGATCY 1 cut(s) 1303
BsuRI GGCC 3 cut(s) 5, 80, 655
BtgI CCRYGG 1 cut(s) 864
BtsCI GGATG 3 cut(s) 177, 482, 1114
BtsI GCAGTG 1 cut(s) 1216
BtsIMutI CAGTG 3 cut(s) 330, 444, 1216
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 2 cut(s) 158, 1269
CciI TCATGA 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 776, 853
CsiI ACCWGGT 1 cut(s) 232
CspCI CAANNNNNGTGG 2 cut(s) 1272, 1307
CviAII CATG 7 cut(s) 221, 460, 629, 749, 865, 1054, 1394
DdeI CTNAG 4 cut(s) 527, 1015, 1346, 1377
DpnI GATC 5 cut(s) 219, 570, 598, 1058, 1305
DpnII GATC 5 cut(s) 217, 568, 596, 1056, 1303
DraI TTTAAA 1 cut(s) 612
EaeI YGGCCR 1 cut(s) 3
Eco105I TACGTA 1 cut(s) 387
Eco130I CCWWGG 2 cut(s) 488, 864
Eco31I GGTCTC 1 cut(s) 1373
Eco47I GGWCC 2 cut(s) 776, 853
Eco57I CTGAAG 2 cut(s) 95, 1215
Eco91I GGTNACC 1 cut(s) 995
EcoO109I RGGNCCY 1 cut(s) 853
EcoO65I GGTNACC 1 cut(s) 995
EcoRI GAATTC 2 cut(s) 394, 1315
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 488, 864
ErhI CCWWGG 2 cut(s) 488, 864
FaeI CATG 7 cut(s) 224, 463, 632, 752, 868, 1057, 1397
FaqI GGGAC 3 cut(s) 127, 799, 1064
FatI CATG 7 cut(s) 220, 459, 628, 748, 864, 1053, 1393
FauNDI CATATG 1 cut(s) 880
FbaI TGATCA 1 cut(s) 1056
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 4 cut(s) 469, 501, 704, 1134
FokI GGATG 3 cut(s) 164, 489, 1101
Fsp4HI GCNGC 4 cut(s) 469, 501, 704, 1134
FspBI CTAG 3 cut(s) 401, 620, 1001
GluI GCNGC 4 cut(s) 469, 501, 704, 1134
HaeIII GGCC 3 cut(s) 5, 80, 655
HapII CCGG 3 cut(s) 6, 687, 809
Hin1II CATG 7 cut(s) 224, 463, 632, 752, 868, 1057, 1397
HincII GTYRAC 2 cut(s) 457, 1110
HindII GTYRAC 2 cut(s) 457, 1110
HinfI GANTC 8 cut(s) 41, 289, 352, 709, 745, 812, 1111, 1171
HpaII CCGG 3 cut(s) 6, 687, 809
HphI GGTGA 4 cut(s) 36, 591, 682, 1007
Hpy166II GTNNAC 7 cut(s) 97, 328, 370, 457, 800, 1085, 1110
Hpy188I TCNGA 6 cut(s) 417, 530, 1010, 1018, 1234, 1347
Hpy188III TCNNGA 1 cut(s) 221
Hpy8I GTNNAC 7 cut(s) 97, 328, 370, 457, 800, 1085, 1110
HpyAV CCTTC 6 cut(s) 236, 830, 844, 1030, 1189, 1348
HpyCH4III ACNGT 4 cut(s) 72, 454, 670, 795
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 6 cut(s) 156, 259, 471, 606, 692, 1209
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 629, 703
HpyF3I CTNAG 4 cut(s) 527, 1015, 1346, 1377
HpySE526I ACGT 1 cut(s) 386
Hsp92II CATG 7 cut(s) 224, 463, 632, 752, 868, 1057, 1397
KpnI GGTACC 2 cut(s) 213, 940
Ksp22I TGATCA 1 cut(s) 1056
Kzo9I GATC 5 cut(s) 217, 568, 596, 1056, 1303
LmnI GCTCC 2 cut(s) 8, 404
Lsp1109I GCAGC 3 cut(s) 455, 487, 1120
MabI ACCWGGT 1 cut(s) 232
MaeI CTAG 3 cut(s) 401, 620, 1001
MaeII ACGT 1 cut(s) 386
MaeIII GTNAC 5 cut(s) 311, 641, 787, 995, 1389
MalI GATC 5 cut(s) 219, 570, 598, 1058, 1305
MboI GATC 5 cut(s) 217, 568, 596, 1056, 1303
MboII GAAGA 6 cut(s) 101, 306, 514, 563, 1180, 1339
MfeI CAATTG 1 cut(s) 783
MflI RGATCY 1 cut(s) 1303
MhlI GDGCHC 1 cut(s) 25
MlyI GAGTC 5 cut(s) 35, 754, 821, 1105, 1165
MmeI TCCRAC 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 873
MseI TTAA 2 cut(s) 611, 714
MslI CAYNNNNRTG 6 cut(s) 591, 753, 926, 1017, 1031, 1119
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 3 cut(s) 6, 687, 809
MspR9I CCNGG 1 cut(s) 234
MunI CAATTG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 500, 629, 703
NcoI CCATGG 1 cut(s) 864
NdeI CATATG 1 cut(s) 880
NdeII GATC 5 cut(s) 217, 568, 596, 1056, 1303
NlaIII CATG 7 cut(s) 224, 463, 632, 752, 868, 1057, 1397
NlaIV GGNNCC 6 cut(s) 211, 276, 514, 855, 938, 1281
NmuCI GTSAC 3 cut(s) 311, 641, 995
NspI RCATGY 1 cut(s) 463
NspV TTCGAA 2 cut(s) 292, 558
PagI TCATGA 1 cut(s) 220
PciI ACATGT 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 873
PfeI GAWTC 3 cut(s) 289, 352, 709
PflMI CCANNNNNTGG 1 cut(s) 884
PkrI GCNGC 4 cut(s) 470, 502, 705, 1135
PleI GAGTC 5 cut(s) 35, 753, 820, 1105, 1165
PpsI GAGTC 5 cut(s) 35, 753, 820, 1105, 1165
Ppu21I YACGTR 1 cut(s) 387
PpuMI RGGWCCY 1 cut(s) 853
PscI ACATGT 1 cut(s) 459
PsiI TTATAA 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 232
PspEI GGTNACC 1 cut(s) 995
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 6 cut(s) 211, 276, 514, 855, 938, 1281
PspPI GGNCC 2 cut(s) 776, 853
PspPPI RGGWCCY 1 cut(s) 853
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 1303
RseI CAYNNNNRTG 6 cut(s) 591, 753, 926, 1017, 1031, 1119
SaqAI TTAA 2 cut(s) 611, 714
SatI GCNGC 4 cut(s) 469, 501, 704, 1134
Sau3AI GATC 5 cut(s) 217, 568, 596, 1056, 1303
Sau96I GGNCC 2 cut(s) 776, 853
SbfI CCTGCAGG 1 cut(s) 261
ScaI AGTACT 1 cut(s) 1228
SchI GAGTC 5 cut(s) 35, 754, 821, 1105, 1165
ScrFI CCNGG 1 cut(s) 234
SdaI CCTGCAGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 25
SexAI ACCWGGT 1 cut(s) 232
SfcI CTRYAG 1 cut(s) 257
SfuI TTCGAA 2 cut(s) 292, 558
SinI GGWCC 2 cut(s) 776, 853
SmiMI CAYNNNNRTG 6 cut(s) 591, 753, 926, 1017, 1031, 1119
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
SnaBI TACGTA 1 cut(s) 387
Sse8387I CCTGCAGG 1 cut(s) 261
SsiI CCGC 4 cut(s) 272, 543, 701, 704
SspI AATATT 1 cut(s) 676
SspMI CTAG 3 cut(s) 401, 620, 1001
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 2 cut(s) 488, 864
TaaI ACNGT 4 cut(s) 72, 454, 670, 795
TaiI ACGT 1 cut(s) 389
TaqI TCGA 3 cut(s) 132, 292, 558
TatI WGTACW 5 cut(s) 266, 369, 819, 1082, 1226
TauI GCSGC 1 cut(s) 706
TfiI GAWTC 3 cut(s) 289, 352, 709
Tru1I TTAA 2 cut(s) 611, 714
Tru9I TTAA 2 cut(s) 611, 714
TscAI CASTG 3 cut(s) 330, 444, 1216
TseFI GTSAC 3 cut(s) 311, 641, 995
TseI GCWGC 3 cut(s) 468, 500, 1133
Tsp45I GTSAC 3 cut(s) 311, 641, 995
TspDTI ATGAA 4 cut(s) 321, 575, 1049, 1259
TspRI CASTG 3 cut(s) 330, 444, 1216
Van91I CCANNNNNTGG 1 cut(s) 884
VpaK11BI GGWCC 2 cut(s) 776, 853
XapI RAATTY 4 cut(s) 394, 946, 1219, 1315
XceI RCATGY 1 cut(s) 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 444
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 873
XspI CTAG 3 cut(s) 401, 620, 1001
ZrmI AGTACT 1 cut(s) 1228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.