Rorug02G0068200

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004833
Physical Location & Seq
Forward (+)
5325515 .. 5327559
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0068200.1

Sequence Viewer

Length: 1164 bp
ATGATGATCGGCGAACCTCACCGTTCGAATCCGACCATCCACGTCCCCCCGTGGCTCACCCTCGACGATCCAACGGCCGAGATGTACTCCCCCTACCCCCTGAGCGGCGTCTCCGTCAACTCTGACGTCACCAATGCCAACAGCCCCTACCACCTCAACGAGGCTCTCGCCACTCTCCAGCGCTACTTGCCTTCCAACGAGGTTTCCGACCCGGACCCGGACTCCGACTCCGAGATTTCGGGTATGGACTCGGACTCTCCGGTCGACGCCTACTCGTGCGACCATTTTCGGATGTTTGAGTTCAAGGTCCGTCGCTGCGCACGTGGCAGGTCCCATGACTGGACCGACTGCCCCTTCGCCCACCCGGGCGAGAAGGCCCGCCGCCGCGACCCGAGGAAGTACCACTATTCGGGTTCCGCCTGCCCCGATTTCCGAAAGGGCCACTGCAAGAAGGGAGACTCCTGCGAGTTCGCTCACGGCGTTTTCGAGTGCTGGCTCCACCCTGCGCGTTATCGGACTCAGCCCTGTAAAGATGGTACTAGCTGCAAGAGGAGAGTGTGCTTCTTCGCTCACACGCCGGAGCAGCTTAGGGTTTTGCCCCAGCAGAGTCCCCGAGGGTCAACCTCGGCCGAGTCGTACGACGGGTCGCCTCTGAGGCAGGCCATGGAGGCGGCGTCGCTGGGGAAAGGTATGCCGTTCATGTCGTCGCCGCCGCCGTCGATTTCGCCGGATGATTCGCCGCCGGTGTCTCCGATGACCAGGTCGGTGAGCCGGTCTCTGGGTTCGAGCTCGATTAATGAAATGGTGGCGTCGCTGAGGAACTTGCAGCTGGGGAAGGTCAAGTCTCTGCCCACTTCATGGAATGTTCAAGTCGGGTCGCCCGGGTTCGTTTCTCCACGCGGGTCGATGCTCCGACCCGGGTTCTGTAGCCTGCCGTCGACTCCGACCCGGATTCCGACCCGCTCTGCAATTGGGTTTCTGGATTTCTGTGACGAGGGTTGCGAGGAGGAGCCGCCGATGGAGAGGGTGGAGTCTGGGAGGGACTTGAGGGCCAAGATGTTCGAGAGGTTGAGGGAGGAGAATTCTCTGGAGCGGGTCGACCCGACCCAGGCACAGGCTTCCGGTGCTCCGGATGTTGGATGGGTTTCTGAGCTGGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

387

Amino Acids

42.86

Weight (kDa)

6.15

Isoelectric Point (pI)

68.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_AtC3H23 PF25512 95 - 128 2.3e-20 AtC3H23-like, CCCH zinc finger
zf-CCCH PF00642 139 - 160 6.2e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 129
Acc16I TGCGCA 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 318
AccB7I CCANNNNNTGG 1 cut(s) 858
AccBSI CCGCTC 3 cut(s) 105, 963, 1093
AccI GTMKAC 3 cut(s) 264, 938, 1098
AccII CGCG 3 cut(s) 387, 508, 900
AccIII TCCGGA 1 cut(s) 1129
AclWI GGATC 1 cut(s) 62
AcoI YGGCCR 2 cut(s) 75, 627
AcsI RAATTY 1 cut(s) 1081
AcvI CACGTG 1 cut(s) 323
AcyI GRCGYC 5 cut(s) 108, 126, 267, 674, 809
AfaI GTAC 4 cut(s) 86, 401, 538, 638
AfeI AGCGCT 1 cut(s) 182
AgsI TTSAA 2 cut(s) 304, 869
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 2 cut(s) 758, 1107
AluBI AGCT 5 cut(s) 543, 586, 789, 829, 1153
AluI AGCT 5 cut(s) 543, 586, 789, 829, 1153
Alw21I GWGCWC 2 cut(s) 791, 1129
Alw26I GTCTC 5 cut(s) 115, 450, 753, 780, 849
AlwI GGATC 1 cut(s) 62
Ama87I CYCGRG 5 cut(s) 364, 391, 612, 881, 917
Aor13HI TCCGGA 1 cut(s) 1129
Aor51HI AGCGCT 1 cut(s) 182
AoxI GGCC 6 cut(s) 75, 375, 439, 627, 660, 1050
ApeKI GCWGC 4 cut(s) 315, 543, 583, 826
ApoI RAATTY 1 cut(s) 1081
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AseI ATTAAT 1 cut(s) 795
AspLEI GCGC 3 cut(s) 183, 320, 508
AspS9I GGNCC 7 cut(s) 214, 307, 330, 342, 376, 439, 1050
AsuC2I CCSGG 9 cut(s) 212, 218, 365, 366, 882, 883, 918, 919, 949
AsuHPI GGTGA 4 cut(s) 11, 49, 121, 778
AsuII TTCGAA 1 cut(s) 26
AvaI CYCGRG 5 cut(s) 364, 391, 612, 881, 917
AvaII GGWCC 4 cut(s) 214, 307, 330, 342
BanII GRGCYC 1 cut(s) 791
BauI CACGAG 1 cut(s) 274
BbrPI CACGTG 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 791, 1129
BbvCI CCTCAGC 1 cut(s) 815
BbvI GCAGC 4 cut(s) 302, 530, 595, 838
BccI CCATC 4 cut(s) 44, 527, 1012, 1134
BceAI ACGGC 5 cut(s) 90, 493, 679, 700, 919
BciT130I CCWGG 2 cut(s) 760, 1109
BcnI CCSGG 9 cut(s) 212, 218, 365, 366, 882, 883, 918, 919, 949
BcoDI GTCTC 5 cut(s) 115, 450, 753, 780, 849
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 925
BfoI RGCGCY 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 318
BglI GCCNNNNNGGC 2 cut(s) 655, 668
Bme18I GGWCC 4 cut(s) 214, 307, 330, 342
BmeT110I CYCGRG 5 cut(s) 364, 391, 612, 881, 917
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 7 cut(s) 214, 307, 330, 342, 376, 439, 1050
BmiI GGNNCC 5 cut(s) 216, 332, 415, 497, 1011
BmsI GCATC 1 cut(s) 897
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 760, 792
BpmI CTGGAG 2 cut(s) 161, 1109
Bpu10I CCTNAGC 3 cut(s) 101, 587, 815
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 1066
BpuMI CCSGG 9 cut(s) 212, 218, 365, 366, 882, 883, 918, 919, 949
BsaAI YACGTR 1 cut(s) 323
BsaHI GRCGYC 5 cut(s) 108, 126, 267, 674, 809
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 9 cut(s) 50, 364, 392, 613, 624, 663, 881, 917, 1107
BsaWI WCCGGW 3 cut(s) 259, 1121, 1129
BsaXI ACNNNNNCTCC 6 cut(s) 206, 212, 236, 242, 659, 689
Bse118I RCCGGY 2 cut(s) 742, 771
Bse1I ACTGG 1 cut(s) 344
BseAI TCCGGA 1 cut(s) 1129
BseBI CCWGG 2 cut(s) 760, 1109
BseDI CCNNGG 9 cut(s) 50, 364, 392, 613, 624, 663, 881, 917, 1107
BseGI GGATG 5 cut(s) 36, 297, 736, 1138, 1145
BseMII CTCAG 5 cut(s) 92, 533, 644, 806, 1140
BseNI ACTGG 1 cut(s) 344
BseRI GAGGAG 4 cut(s) 565, 1019, 1022, 1091
BseX3I CGGCCG 2 cut(s) 75, 627
BseXI GCAGC 4 cut(s) 302, 530, 595, 838
BseYI CCCAGC 3 cut(s) 600, 679, 829
Bsh1236I CGCG 3 cut(s) 387, 508, 900
Bsh1285I CGRYCG 3 cut(s) 78, 264, 630
BshFI GGCC 6 cut(s) 77, 377, 441, 629, 662, 1052
BsiEI CGRYCG 3 cut(s) 78, 264, 630
BsiHKAI GWGCWC 2 cut(s) 791, 1129
BsiHKCI CYCGRG 5 cut(s) 364, 391, 612, 881, 917
BsiWI CGTACG 1 cut(s) 636
BslFI GGGAC 4 cut(s) 29, 316, 594, 1055
BsmAI GTCTC 5 cut(s) 115, 450, 753, 780, 849
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 4 cut(s) 29, 316, 594, 1055
BsnI GGCC 6 cut(s) 77, 377, 441, 629, 662, 1052
Bso31I GGTCTC 1 cut(s) 780
BsoBI CYCGRG 5 cut(s) 364, 391, 612, 881, 917
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 791, 1129
Bsp13I TCCGGA 1 cut(s) 1129
Bsp143I GATC 2 cut(s) 6, 67
Bsp19I CCATGG 1 cut(s) 663
BspANI GGCC 6 cut(s) 77, 377, 441, 629, 662, 1052
BspCNI CTCAG 5 cut(s) 93, 532, 645, 807, 1141
BspEI TCCGGA 1 cut(s) 1129
BspFNI CGCG 3 cut(s) 387, 508, 900
BspLI GGNNCC 5 cut(s) 216, 332, 415, 497, 1011
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 1 cut(s) 62
BspT104I TTCGAA 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 780
BsrBI CCGCTC 3 cut(s) 105, 963, 1093
BsrFI RCCGGY 2 cut(s) 742, 771
BsrI ACTGG 1 cut(s) 344
BssAI RCCGGY 2 cut(s) 742, 771
BssECI CCNNGG 9 cut(s) 50, 364, 392, 613, 624, 663, 881, 917, 1107
BssMI GATC 2 cut(s) 6, 67
BssNI GRCGYC 5 cut(s) 108, 126, 267, 674, 809
BssSI CACGAG 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 760, 1109
Bst4CI ACNGT 1 cut(s) 23
BstACI GRCGYC 5 cut(s) 108, 126, 267, 674, 809
BstBAI YACGTR 1 cut(s) 323
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 5 cut(s) 379, 421, 494, 660, 932
BstDEI CTNAG 6 cut(s) 101, 519, 587, 653, 815, 1149
BstDSI CCRYGG 2 cut(s) 50, 663
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 5 cut(s) 36, 297, 736, 1138, 1145
BstFNI CGCG 3 cut(s) 387, 508, 900
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 3 cut(s) 183, 320, 508
BstKTI GATC 2 cut(s) 9, 70
BstMAI GTCTC 5 cut(s) 115, 450, 753, 780, 849
BstMBI GATC 2 cut(s) 6, 67
BstMCI CGRYCG 3 cut(s) 78, 264, 630
BstMWI GCNNNNNNNGC 6 cut(s) 187, 324, 583, 655, 668, 1124
BstNI CCWGG 2 cut(s) 760, 1109
BstSFI CTRYAG 1 cut(s) 925
BstUI CGCG 3 cut(s) 387, 508, 900
BstV1I GCAGC 4 cut(s) 302, 530, 595, 838
BstZI CGGCCG 2 cut(s) 75, 627
BsuRI GGCC 6 cut(s) 77, 377, 441, 629, 662, 1052
BtgI CCRYGG 2 cut(s) 50, 663
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 5 cut(s) 36, 297, 736, 1138, 1145
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 442
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 5 cut(s) 379, 421, 494, 660, 932
CfoI GCGC 3 cut(s) 183, 320, 508
Cfr10I RCCGGY 2 cut(s) 742, 771
Cfr13I GGNCC 7 cut(s) 214, 307, 330, 342, 376, 439, 1050
Cfr9I CCCGGG 3 cut(s) 364, 881, 917
CseI GACGC 4 cut(s) 97, 275, 663, 798
CsiI ACCWGGT 1 cut(s) 758
Csp6I GTAC 4 cut(s) 85, 400, 537, 637
CviAII CATG 4 cut(s) 335, 664, 700, 858
CviQI GTAC 4 cut(s) 85, 400, 537, 637
DdeI CTNAG 6 cut(s) 101, 519, 587, 653, 815, 1149
DpnI GATC 2 cut(s) 8, 69
DpnII GATC 2 cut(s) 6, 67
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 2 cut(s) 75, 627
EagI CGGCCG 2 cut(s) 75, 627
EciI GGCGGA 1 cut(s) 406
Ecl136II GAGCTC 1 cut(s) 789
EclXI CGGCCG 2 cut(s) 75, 627
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 4 cut(s) 214, 307, 330, 342
Eco47III AGCGCT 1 cut(s) 182
Eco52I CGGCCG 2 cut(s) 75, 627
Eco53kI GAGCTC 1 cut(s) 789
Eco72I CACGTG 1 cut(s) 323
Eco88I CYCGRG 5 cut(s) 364, 391, 612, 881, 917
EcoICRI GAGCTC 1 cut(s) 789
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 330
EcoRI GAATTC 1 cut(s) 1081
EcoRII CCWGG 2 cut(s) 758, 1107
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 1 cut(s) 663
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 4 cut(s) 338, 667, 703, 861
FaiI YATR 6 cut(s) 245, 336, 665, 692, 701, 859
FaqI GGGAC 4 cut(s) 29, 316, 594, 1055
FatI CATG 4 cut(s) 334, 663, 699, 857
FauI CCCGC 4 cut(s) 386, 893, 968, 1086
FblI GTMKAC 3 cut(s) 264, 938, 1098
FokI GGATG 5 cut(s) 23, 304, 743, 1145, 1152
FriOI GRGCYC 1 cut(s) 791
FspBI CTAG 1 cut(s) 540
FspI TGCGCA 1 cut(s) 319
GlaI GCGC 3 cut(s) 182, 319, 507
GsaI CCCAGC 3 cut(s) 604, 683, 833
GsuI CTGGAG 2 cut(s) 161, 1109
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 6 cut(s) 77, 377, 441, 629, 662, 1052
HgaI GACGC 4 cut(s) 97, 275, 663, 798
HhaI GCGC 3 cut(s) 183, 320, 508
Hin1I GRCGYC 5 cut(s) 108, 126, 267, 674, 809
Hin1II CATG 4 cut(s) 338, 667, 703, 861
Hin6I GCGC 3 cut(s) 181, 318, 506
HinP1I GCGC 3 cut(s) 181, 318, 506
HincII GTYRAC 5 cut(s) 118, 265, 621, 939, 1099
HindII GTYRAC 5 cut(s) 118, 265, 621, 939, 1099
HphI GGTGA 4 cut(s) 11, 49, 121, 778
Hpy166II GTNNAC 5 cut(s) 118, 265, 621, 939, 1099
Hpy188III TCNNGA 4 cut(s) 980, 1063, 1088, 1130
Hpy8I GTNNAC 5 cut(s) 118, 265, 621, 939, 1099
Hpy99I CGWCG 9 cut(s) 68, 269, 315, 644, 679, 709, 721, 814, 940
HpyAV CCTTC 5 cut(s) 201, 364, 367, 445, 829
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4IV ACGT 3 cut(s) 42, 126, 322
HpyCH4V TGCA 4 cut(s) 447, 546, 826, 968
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 324, 583, 655, 668, 1124
HpyF3I CTNAG 6 cut(s) 101, 519, 587, 653, 815, 1149
HpySE526I ACGT 3 cut(s) 42, 126, 322
Hsp92I GRCGYC 5 cut(s) 108, 126, 267, 674, 809
Hsp92II CATG 4 cut(s) 338, 667, 703, 861
HspAI GCGC 3 cut(s) 181, 318, 506
Kpn2I TCCGGA 1 cut(s) 1129
Kzo9I GATC 2 cut(s) 6, 67
LmnI GCTCC 6 cut(s) 501, 580, 915, 1009, 1090, 1132
Lsp1109I GCAGC 4 cut(s) 302, 530, 595, 838
LweI GCATC 1 cut(s) 897
MabI ACCWGGT 1 cut(s) 758
MaeI CTAG 1 cut(s) 540
MaeII ACGT 3 cut(s) 42, 126, 322
MaeIII GTNAC 2 cut(s) 127, 989
MalI GATC 2 cut(s) 8, 69
MbiI CCGCTC 3 cut(s) 105, 963, 1093
MboI GATC 2 cut(s) 6, 67
MboII GAAGA 1 cut(s) 556
MfeI CAATTG 1 cut(s) 969
MhlI GDGCHC 2 cut(s) 791, 1129
MluCI AATT 3 cut(s) 969, 1081, 1159
MmeI TCCRAC 9 cut(s) 56, 95, 219, 231, 249, 937, 968, 980, 1117
MroI TCCGGA 1 cut(s) 1129
MseI TTAA 1 cut(s) 795
MspA1I CMGCKG 1 cut(s) 829
MunI CAATTG 1 cut(s) 969
MvaI CCWGG 2 cut(s) 760, 1109
MvnI CGCG 3 cut(s) 387, 508, 900
MwoI GCNNNNNNNGC 6 cut(s) 187, 324, 583, 655, 668, 1124
NciI CCSGG 9 cut(s) 212, 218, 365, 366, 882, 883, 918, 919, 949
NcoI CCATGG 1 cut(s) 663
NdeII GATC 2 cut(s) 6, 67
NlaIII CATG 4 cut(s) 338, 667, 703, 861
NlaIV GGNNCC 5 cut(s) 216, 332, 415, 497, 1011
NmeAIII GCCGAG 3 cut(s) 103, 605, 655
NmuCI GTSAC 2 cut(s) 127, 989
NsbI TGCGCA 1 cut(s) 319
NspV TTCGAA 1 cut(s) 26
PcsI WCGNNNNNNNCGW 7 cut(s) 204, 319, 477, 483, 632, 713, 999
PfeI GAWTC 3 cut(s) 28, 734, 952
Pfl23II CGTACG 1 cut(s) 636
PflFI GACNNNGTC 1 cut(s) 760
PflMI CCANNNNNTGG 1 cut(s) 858
PmaCI CACGTG 1 cut(s) 323
PmlI CACGTG 1 cut(s) 323
Ppu21I YACGTR 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 330
PshBI ATTAAT 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 791
Psp5II RGGWCCY 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 758, 1107
PspCI CACGTG 1 cut(s) 323
PspFI CCCAGC 3 cut(s) 600, 679, 829
PspGI CCWGG 2 cut(s) 758, 1107
PspLI CGTACG 1 cut(s) 636
PspN4I GGNNCC 5 cut(s) 216, 332, 415, 497, 1011
PspPI GGNCC 7 cut(s) 214, 307, 330, 342, 376, 439, 1050
PspPPI RGGWCCY 1 cut(s) 330
PsyI GACNNNGTC 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 829
RsaI GTAC 4 cut(s) 86, 401, 538, 638
RsaNI GTAC 4 cut(s) 85, 400, 537, 637
SacI GAGCTC 1 cut(s) 791
SalI GTCGAC 3 cut(s) 263, 937, 1097
SaqAI TTAA 1 cut(s) 795
Sau3AI GATC 2 cut(s) 6, 67
Sau96I GGNCC 7 cut(s) 214, 307, 330, 342, 376, 439, 1050
SduI GDGCHC 2 cut(s) 791, 1129
SexAI ACCWGGT 1 cut(s) 758
SfaNI GCATC 1 cut(s) 897
SfcI CTRYAG 1 cut(s) 925
SfuI TTCGAA 1 cut(s) 26
SgrAI CRCCGGYG 1 cut(s) 742
SinI GGWCC 4 cut(s) 214, 307, 330, 342
SmaI CCCGGG 3 cut(s) 366, 883, 919
SmlI CTYRAG 1 cut(s) 1045
SmoI CTYRAG 1 cut(s) 1045
Sse9I AATT 3 cut(s) 969, 1081, 1159
SspMI CTAG 1 cut(s) 540
SstI GAGCTC 1 cut(s) 791
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 1 cut(s) 23
TaiI ACGT 3 cut(s) 45, 129, 325
TaqII GACCGA 1 cut(s) 359
TasI AATT 3 cut(s) 969, 1081, 1159
TatI WGTACW 1 cut(s) 84
TauI GCSGC 8 cut(s) 108, 384, 387, 674, 712, 715, 742, 1015
TfiI GAWTC 3 cut(s) 28, 734, 952
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 2 cut(s) 127, 989
TseI GCWGC 4 cut(s) 315, 543, 583, 826
Tsp45I GTSAC 2 cut(s) 127, 989
TspDTI ATGAA 3 cut(s) 688, 813, 846
TspGWI ACGGA 2 cut(s) 103, 299
TspMI CCCGGG 3 cut(s) 364, 881, 917
TspRI CASTG 1 cut(s) 449
Tth111I GACNNNGTC 1 cut(s) 760
Van91I CCANNNNNTGG 1 cut(s) 858
VpaK11BI GGWCC 4 cut(s) 214, 307, 330, 342
VspI ATTAAT 1 cut(s) 795
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 1081
XmaI CCCGGG 3 cut(s) 364, 881, 917
XmiI GTMKAC 3 cut(s) 264, 938, 1098
XspI CTAG 1 cut(s) 540
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.