FvH4_1g12491

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002146
Physical Location & Seq
Forward (+)
6865602 .. 6871628
6027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12491.t1

Sequence Viewer

Length: 1059 bp
ATGAGCTTAAGCTTTTTCATCCAATTTGCTTGCTTAGTGTCGTCCTTGCTTGTATCCGTTACCCTAGTTCATGCTCAGACAAGGGTTTTCGATGTGACAAACTATGGTGGTCGTGGAGACGGAATAACAGATAACAGCAAGGCCTTCATTGATGCATGGGACGGAGCTTGTCAAAACATAGGAGGAGGTATGGTGCTGTTCCCACAAGGCATCTTCATGGTGGCTCCGGTGGTTCTTCTAGGTCCATGTAAGGGATCAATGGAATTGCGAATTCAGGGTACCCTACTGGCTTCTAGGAAGCTCAGTGATTTCCTTGGTATTGACCATTGGATCACCATACAATACGTTAACGGGTTGCTGATTAGTGGTGGAGGAACCCTAGATGGCGTCGGAGCCGATGGTTGGCCCTATAATGATTGCCTCCAACATCCAGGCAGTTGCAAGCGACTCCCTGCTGACGTGCTTGTTAGCAACGTCAACATATTAGCTCCGGGAAACAGTCCCAACACCGATGGAATCCACATTGGCAGCTCACAGAACATCTATATCGTGCATACAAACATATCGACCGGAGACGATTGCGTCTCCATCTCCTCCGGATCACAATACATAAACGTCACCGGTGTCCAATGCGGTCCAGGCCATGGAATAAGCATCGGAAGCCTCGGCAAAGATGCCGGTGACACTGTTAACGACTTGTATGTAACACACTGCAGATTCGTGGGTACTCAGAATGGTGTGAGAATCAAAACATGGGCAGATGCTAAACCGGGAACTGTTTCGCGTGTTAGCTTTGAGCACCTTGAAATAGTTAATGTGGGCAATCCCATCATTATTGATCAAGAATATTGCCCTGGTGGTGGTTGTAGTACACAGGTGTCCTCCAAAGTCCAGATTGGGGATGTTAAGTTCAATGACATTAGAGGCAGTTCTGCCACAAACGAGGCAGTTGTCCTGAAATGCAGCCAAAGTGTACCTTGTCAAGATATCGAGCTGACGGACATCGACCTAATTAAAGAACGGAGGACCTGGAGGACCGGCGGTTTCATTGTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

353

Amino Acids

37.37

Weight (kDa)

5.41

Isoelectric Point (pI)

28.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 57 - 152 8.9e-09 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 153 - 339 4.5e-65 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 278
AccB7I CCANNNNNTGG 1 cut(s) 644
AccII CGCG 1 cut(s) 784
AccIII TCCGGA 1 cut(s) 596
AciI CCGC 2 cut(s) 633, 1041
AclWI GGATC 3 cut(s) 262, 338, 607
AcsI RAATTY 1 cut(s) 270
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 4 cut(s) 280, 727, 871, 975
AfiI CCNNNNNNNGG 5 cut(s) 251, 402, 644, 860, 898
AflII CTTAAG 1 cut(s) 7
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 2 cut(s) 806, 913
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 4 cut(s) 430, 637, 853, 1028
AloI GAACNNNNNNTCC 2 cut(s) 893, 925
AluBI AGCT 8 cut(s) 6, 12, 167, 301, 488, 531, 792, 994
AluI AGCT 8 cut(s) 6, 12, 167, 301, 488, 531, 792, 994
Alw21I GWGCWC 1 cut(s) 801
Alw26I GTCTC 3 cut(s) 111, 567, 589
AlwI GGATC 3 cut(s) 262, 338, 607
Aor13HI TCCGGA 1 cut(s) 596
AoxI GGCC 3 cut(s) 141, 404, 640
ApeKI GCWGC 2 cut(s) 528, 963
ApoI RAATTY 1 cut(s) 270
AsiGI ACCGGT 1 cut(s) 620
Asp700I GAANNNNTTC 1 cut(s) 778
Asp718I GGTACC 1 cut(s) 278
AspS9I GGNCC 5 cut(s) 242, 405, 635, 1026, 1035
AsuC2I CCSGG 2 cut(s) 492, 771
AsuHPI GGTGA 3 cut(s) 325, 610, 692
AvaII GGWCC 4 cut(s) 242, 635, 1026, 1035
BanI GGYRCC 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 801
BbvI GCAGC 2 cut(s) 540, 975
BccI CCATC 5 cut(s) 377, 392, 506, 596, 836
BciT130I CCWGG 4 cut(s) 432, 639, 855, 1030
BciVI GTATCC 1 cut(s) 64
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 2 cut(s) 492, 771
BcoDI GTCTC 3 cut(s) 111, 567, 589
BfaI CTAG 4 cut(s) 65, 239, 294, 380
BfmI CTRYAG 1 cut(s) 712
BfrI CTTAAG 1 cut(s) 7
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 2 cut(s) 529, 964
BlsI GCNGC 2 cut(s) 530, 965
Bme1390I CCNGG 6 cut(s) 432, 492, 639, 771, 855, 1030
Bme18I GGWCC 4 cut(s) 242, 635, 1026, 1035
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 5 cut(s) 242, 405, 635, 1026, 1035
BmiI GGNNCC 4 cut(s) 225, 280, 376, 394
BmrFI CCNGG 6 cut(s) 432, 492, 639, 771, 855, 1030
BmsI GCATC 5 cut(s) 142, 219, 663, 664, 751
BpmI CTGGAG 1 cut(s) 1051
BpuMI CCSGG 2 cut(s) 492, 771
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 4 cut(s) 313, 643, 664, 853
BsaWI WCCGGW 4 cut(s) 226, 569, 596, 620
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 5 cut(s) 251, 402, 644, 860, 898
Bse118I RCCGGY 3 cut(s) 620, 677, 1037
Bse1I ACTGG 1 cut(s) 291
BseAI TCCGGA 1 cut(s) 596
BseBI CCWGG 4 cut(s) 432, 639, 855, 1030
BseDI CCNNGG 4 cut(s) 313, 643, 664, 853
BseGI GGATG 3 cut(s) 18, 427, 907
BseLI CCNNNNNNNGG 5 cut(s) 251, 402, 644, 860, 898
BseMII CTCAG 3 cut(s) 89, 316, 743
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 2 cut(s) 198, 583
BseXI GCAGC 2 cut(s) 540, 975
Bsh1236I CGCG 1 cut(s) 784
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 3 cut(s) 143, 406, 642
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 570
BsiHKAI GWGCWC 1 cut(s) 801
BsiSI CCGG 8 cut(s) 227, 491, 570, 597, 621, 678, 770, 1038
BslFI GGGAC 2 cut(s) 173, 486
BslI CCNNNNNNNGG 5 cut(s) 251, 402, 644, 860, 898
BsmAI GTCTC 3 cut(s) 111, 567, 589
BsmBI CGTCTC 3 cut(s) 111, 567, 589
BsmFI GGGAC 2 cut(s) 173, 486
BsnI GGCC 3 cut(s) 143, 406, 642
Bsp1286I GDGCHC 1 cut(s) 801
Bsp13I TCCGGA 1 cut(s) 596
Bsp143I GATC 4 cut(s) 254, 330, 599, 838
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 2 cut(s) 633, 1041
BspANI GGCC 3 cut(s) 143, 406, 642
BspCNI CTCAG 3 cut(s) 88, 315, 742
BspEI TCCGGA 1 cut(s) 596
BspFNI CGCG 1 cut(s) 784
BspLI GGNNCC 4 cut(s) 225, 280, 376, 394
BspMAI CTGCAG 1 cut(s) 716
BspPI GGATC 3 cut(s) 262, 338, 607
BspT107I GGYRCC 1 cut(s) 278
BspTI CTTAAG 1 cut(s) 7
BsrFI RCCGGY 3 cut(s) 620, 677, 1037
BsrI ACTGG 1 cut(s) 291
BssAI RCCGGY 3 cut(s) 620, 677, 1037
BssECI CCNNGG 4 cut(s) 313, 643, 664, 853
BssMI GATC 4 cut(s) 254, 330, 599, 838
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 2 cut(s) 313, 643
Bst2UI CCWGG 4 cut(s) 432, 639, 855, 1030
Bst4CI ACNGT 3 cut(s) 500, 688, 778
BstACI GRCGYC 1 cut(s) 387
BstAFI CTTAAG 1 cut(s) 7
BstC8I GCNNGC 2 cut(s) 31, 443
BstDEI CTNAG 4 cut(s) 34, 75, 302, 729
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 3 cut(s) 18, 427, 907
BstFNI CGCG 1 cut(s) 784
BstKTI GATC 4 cut(s) 257, 333, 602, 841
BstMAI GTCTC 3 cut(s) 111, 567, 589
BstMBI GATC 4 cut(s) 254, 330, 599, 838
BstMCI CGRYCG 1 cut(s) 570
BstMWI GCNNNNNNNGC 2 cut(s) 639, 660
BstNI CCWGG 4 cut(s) 432, 639, 855, 1030
BstSCI CCNGG 6 cut(s) 430, 490, 637, 769, 853, 1028
BstSFI CTRYAG 1 cut(s) 712
BstUI CGCG 1 cut(s) 784
BstV1I GCAGC 2 cut(s) 540, 975
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 3 cut(s) 143, 406, 642
BtgI CCRYGG 1 cut(s) 643
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 3 cut(s) 18, 427, 907
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 3 cut(s) 310, 684, 709
Cac8I GCNNGC 2 cut(s) 31, 443
Cfr10I RCCGGY 3 cut(s) 620, 677, 1037
Cfr13I GGNCC 5 cut(s) 242, 405, 635, 1026, 1035
CseI GACGC 2 cut(s) 376, 571
Csp6I GTAC 4 cut(s) 279, 726, 870, 974
CspAI ACCGGT 1 cut(s) 620
CviAII CATG 6 cut(s) 71, 156, 217, 246, 644, 753
CviQI GTAC 4 cut(s) 279, 726, 870, 974
DdeI CTNAG 4 cut(s) 34, 75, 302, 729
DpnI GATC 4 cut(s) 256, 332, 601, 840
DpnII GATC 4 cut(s) 254, 330, 599, 838
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
Eco130I CCWWGG 2 cut(s) 313, 643
Eco147I AGGCCT 1 cut(s) 143
Eco32I GATATC 1 cut(s) 988
Eco47I GGWCC 4 cut(s) 242, 635, 1026, 1035
EcoO109I RGGNCCY 1 cut(s) 1026
EcoRI GAATTC 1 cut(s) 270
EcoRII CCWGG 4 cut(s) 430, 637, 853, 1028
EcoRV GATATC 1 cut(s) 988
EcoT14I CCWWGG 2 cut(s) 313, 643
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 313, 643
Esp3I CGTCTC 3 cut(s) 111, 567, 589
FaeI CATG 6 cut(s) 74, 159, 220, 249, 647, 756
FalI AAGNNNNNCTT 2 cut(s) 961, 993
FaqI GGGAC 2 cut(s) 173, 486
FatI CATG 6 cut(s) 70, 155, 216, 245, 643, 752
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 2 cut(s) 529, 964
FokI GGATG 3 cut(s) 5, 414, 914
Fsp4HI GCNGC 2 cut(s) 529, 964
FspBI CTAG 4 cut(s) 65, 239, 294, 380
GluI GCNGC 2 cut(s) 529, 964
GsuI CTGGAG 1 cut(s) 1051
HaeIII GGCC 3 cut(s) 143, 406, 642
HapII CCGG 8 cut(s) 227, 491, 570, 597, 621, 678, 770, 1038
HgaI GACGC 2 cut(s) 376, 571
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 6 cut(s) 74, 159, 220, 249, 647, 756
HincII GTYRAC 3 cut(s) 349, 478, 691
HindII GTYRAC 3 cut(s) 349, 478, 691
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 4 cut(s) 447, 516, 717, 744
HpaI GTTAAC 2 cut(s) 349, 691
HpaII CCGG 8 cut(s) 227, 491, 570, 597, 621, 678, 770, 1038
HphI GGTGA 3 cut(s) 325, 610, 692
Hpy166II GTNNAC 5 cut(s) 349, 478, 691, 872, 974
Hpy188I TCNGA 4 cut(s) 78, 392, 659, 732
Hpy188III TCNNGA 5 cut(s) 597, 842, 892, 955, 983
Hpy8I GTNNAC 5 cut(s) 349, 478, 691, 872, 974
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 3 cut(s) 500, 688, 778
HpyCH4IV ACGT 4 cut(s) 345, 459, 474, 615
HpyCH4V TGCA 5 cut(s) 155, 441, 553, 714, 963
HpyF10VI GCNNNNNNNGC 2 cut(s) 639, 660
HpyF3I CTNAG 4 cut(s) 34, 75, 302, 729
HpySE526I ACGT 4 cut(s) 345, 459, 474, 615
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 6 cut(s) 74, 159, 220, 249, 647, 756
Kpn2I TCCGGA 1 cut(s) 596
KpnI GGTACC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 838
KspAI GTTAAC 2 cut(s) 349, 691
Kzo9I GATC 4 cut(s) 254, 330, 599, 838
LmnI GCTCC 4 cut(s) 164, 229, 392, 493
Lsp1109I GCAGC 2 cut(s) 540, 975
LweI GCATC 5 cut(s) 142, 219, 663, 664, 751
MaeI CTAG 4 cut(s) 65, 239, 294, 380
MaeII ACGT 4 cut(s) 345, 459, 474, 615
MaeIII GTNAC 5 cut(s) 58, 94, 616, 680, 703
MalI GATC 4 cut(s) 256, 332, 601, 840
MboI GATC 4 cut(s) 254, 330, 599, 838
MboII GAAGA 2 cut(s) 205, 227
MhlI GDGCHC 1 cut(s) 801
MluCI AATT 4 cut(s) 23, 263, 270, 1011
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 2 cut(s) 370, 448
Mph1103I ATGCAT 1 cut(s) 157
MroI TCCGGA 1 cut(s) 596
MroXI GAANNNNTTC 1 cut(s) 778
MseI TTAA 6 cut(s) 8, 348, 690, 813, 906, 1014
MslI CAYNNNNRTG 1 cut(s) 215
MspCI CTTAAG 1 cut(s) 7
MspI CCGG 8 cut(s) 227, 491, 570, 597, 621, 678, 770, 1038
MspR9I CCNGG 6 cut(s) 432, 492, 639, 771, 855, 1030
MvaI CCWGG 4 cut(s) 432, 639, 855, 1030
MvnI CGCG 1 cut(s) 784
MwoI GCNNNNNNNGC 2 cut(s) 639, 660
NciI CCSGG 2 cut(s) 492, 771
NcoI CCATGG 1 cut(s) 643
NdeII GATC 4 cut(s) 254, 330, 599, 838
NlaIII CATG 6 cut(s) 74, 159, 220, 249, 647, 756
NlaIV GGNNCC 4 cut(s) 225, 280, 376, 394
NmeAIII GCCGAG 1 cut(s) 645
NmuCI GTSAC 3 cut(s) 94, 616, 680
NsiI ATGCAT 1 cut(s) 157
PceI AGGCCT 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 3 cut(s) 516, 717, 744
PflMI CCANNNNNTGG 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 490
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 2 cut(s) 530, 965
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
PpuMI RGGWCCY 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 1026
Psp6I CCWGG 4 cut(s) 430, 637, 853, 1028
PspGI CCWGG 4 cut(s) 430, 637, 853, 1028
PspN4I GGNNCC 4 cut(s) 225, 280, 376, 394
PspPI GGNCC 5 cut(s) 242, 405, 635, 1026, 1035
PspPPI RGGWCCY 1 cut(s) 1026
PstI CTGCAG 1 cut(s) 716
RsaI GTAC 4 cut(s) 280, 727, 871, 975
RsaNI GTAC 4 cut(s) 279, 726, 870, 974
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 6 cut(s) 8, 348, 690, 813, 906, 1014
SatI GCNGC 2 cut(s) 529, 964
Sau3AI GATC 4 cut(s) 254, 330, 599, 838
Sau96I GGNCC 5 cut(s) 242, 405, 635, 1026, 1035
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 6 cut(s) 432, 492, 639, 771, 855, 1030
SduI GDGCHC 1 cut(s) 801
SfaNI GCATC 5 cut(s) 142, 219, 663, 664, 751
SfcI CTRYAG 1 cut(s) 712
SgrAI CRCCGGYG 1 cut(s) 620
SinI GGWCC 4 cut(s) 242, 635, 1026, 1035
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 4 cut(s) 23, 263, 270, 1011
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 2 cut(s) 633, 1041
SspI AATATT 1 cut(s) 848
SspMI CTAG 4 cut(s) 65, 239, 294, 380
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 6 cut(s) 430, 490, 637, 769, 853, 1028
StyI CCWWGG 2 cut(s) 313, 643
TaaI ACNGT 3 cut(s) 500, 688, 778
TaiI ACGT 4 cut(s) 348, 462, 477, 618
TaqI TCGA 4 cut(s) 90, 566, 990, 1005
TasI AATT 4 cut(s) 23, 263, 270, 1011
TatI WGTACW 1 cut(s) 869
TfiI GAWTC 3 cut(s) 516, 717, 744
Tru1I TTAA 6 cut(s) 8, 348, 690, 813, 906, 1014
Tru9I TTAA 6 cut(s) 8, 348, 690, 813, 906, 1014
TscAI CASTG 3 cut(s) 310, 691, 716
TseFI GTSAC 3 cut(s) 94, 616, 680
TseI GCWGC 2 cut(s) 528, 963
Tsp45I GTSAC 3 cut(s) 94, 616, 680
TspDTI ATGAA 5 cut(s) 7, 59, 136, 205, 1036
TspGWI ACGGA 5 cut(s) 46, 135, 177, 1013, 1036
TspRI CASTG 3 cut(s) 310, 691, 716
Van91I CCANNNNNTGG 1 cut(s) 644
Vha464I CTTAAG 1 cut(s) 7
VpaK11BI GGWCC 4 cut(s) 242, 635, 1026, 1035
XapI RAATTY 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 4 cut(s) 65, 239, 294, 380
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.